Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,579,532	T→C	N99D (AAT→GAT)	SAUSA300_RS07730 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,579,532	0	T	C	100.0%	17.0 / NA	8	N99D (AAT→GAT)	SAUSA300_RS07730	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (0/8);  total (0/8)

ACAACTGCGTCTTGAGCAAATTCTTTATCTTTAAAATTAAACATGTTGTGAAATTCACTATTTTTAAAACTTGATTCAATCGCTTCTTTTATCTCTTCTGATGACACTCCTACTTGATTCGCAATACTCAATCCAAACGCCAACATGTC  >  NC_007793/1579471‑1579619                                                              |                                                                                        gatctcgCGTCTTGAGCAAATTCTTTATCTTTAAAATTAAACATTTTGTGAAATTCTGTATCTTTAAAACTTGATTCAATCGCTTCTTTTATctctcaca                                                   <  1:1498403/94‑5 (MQ=255) cgatcTGCGTCTTGAGCAAATTCTTTATCTTTAAAATTAAACATTTTGTGAAATTCTGTATCTTTAAAACTTGATTCAATCGCTTCTTTTATCTCTTCtg                                                   <  1:4507278/96‑1 (MQ=255) acaacTGCGTCTTGAGCAAATTCTTTATCTTTAAAATTAAACATTTTGTGAAATTCTGTATCTTTAAAACTTGATTCAATCGCTTCTTTTATCTCTTCtg                                                   <  1:1751356/100‑1 (MQ=255)    ctgcggTCTTGAGCAAATTCTTTATCTTTAAAATTAAACATTTTGTGAAATTCTGTATCTTTAAAACTTGATTCAATCGCTTCTTTTATCTCTTCtgatg                                                <  1:6548275/95‑1 (MQ=255)                       ctttatctttaAAATTAAACATTTTGTGAAATTCTGTATCTTTAAAACTTGATTCAATCGCTTCTTTTATCTCTTCTGATGACACTCCTACTTGATTCgt                             <  1:2438980/100‑2 (MQ=255)                        tttatctttaAAATTAAACATTTTGTGAAATTCTGTATCTTTAAAACTTGATTCAATCGCTTCTTTTATCTCTTCTGATGACACTCCTACTTGATTCGCa                            <  1:920560/100‑1 (MQ=255)                                                  gAAATTCTGTATCTTTAAAACTTGATTCAATCGCTTCTTTTATCTCTTCTGATGACACTCCTACTTGATTCGCAATACTCAATCCAAACGCCAACATGta  <  1:4266220/100‑2 (MQ=255)                                                  gAAATTCTGTATCTTTAAAACTTGATTCAATCGCTTCTTTTATCTCTTCTGATGACACTCCTACTTGATTCGCAATACTCAATCCAAACGCCAACATGTc  <  1:6201679/100‑1 (MQ=255)                                                              |                                                                                        ACAACTGCGTCTTGAGCAAATTCTTTATCTTTAAAATTAAACATGTTGTGAAATTCACTATTTTTAAAACTTGATTCAATCGCTTCTTTTATCTCTTCTGATGACACTCCTACTTGATTCGCAATACTCAATCCAAACGCCAACATGTC  >  NC_007793/1579471‑1579619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑