Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,579,515	G→T	N104K (AAC→AAA)	SAUSA300_RS07730 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,579,515	0	G	T	100.0%	16.8 / NA	8	N104K (AAC→AAA)	SAUSA300_RS07730	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (0/8);  total (0/8)

CAGGATAAAATTCTTTGAATATTATCTGTGGTGTGCTAACAACTGCGTCTTGAGCAAATTCTTTATCTTTAAAATTAAACATGTTGTGAAATTCACTATTTTTAAAACTTGATTCAATCGCTTCTTTTATCTCTTCTGATGACACTCCTACTTGATTCGCA  >  NC_007793/1579433‑1579593                                                                                   |                                                                               cgggATAAAATTCTTTGAATATTATCTGTGGTGTACTAACAACTGCGTCTTGAGCAAATTCTTTATCTTTAAAATTAAACATTTTGTGAAATTctgtatg                                                               <  1:863011/98‑7 (MQ=255)   ggATAAAATTCTTTGAATATTATCTGTGGTGTACTAACAACTGCGTCTTGAGCAAATTCTTTATCTTTAAAATTAAACATTTTGTGAAATTctgtatctt                                                             <  1:1205299/100‑9 (MQ=255)                                       gatctcgCGTCTTGAGCAAATTCTTTATCTTTAAAATTAAACATTTTGTGAAATTCTGTATCTTTAAAACTTGATTCAATCGCTTCTTTTATctctcaca                         <  1:1498403/94‑5 (MQ=255)                                       cgatcTGCGTCTTGAGCAAATTCTTTATCTTTAAAATTAAACATTTTGTGAAATTCTGTATCTTTAAAACTTGATTCAATCGCTTCTTTTATCTCTTCtg                         <  1:4507278/96‑1 (MQ=255)                                       acaacTGCGTCTTGAGCAAATTCTTTATCTTTAAAATTAAACATTTTGTGAAATTCTGTATCTTTAAAACTTGATTCAATCGCTTCTTTTATCTCTTCtg                         <  1:1751356/100‑1 (MQ=255)                                          ctgcggTCTTGAGCAAATTCTTTATCTTTAAAATTAAACATTTTGTGAAATTCTGTATCTTTAAAACTTGATTCAATCGCTTCTTTTATCTCTTCtgatg                      <  1:6548275/95‑1 (MQ=255)                                                             ctttatctttaAAATTAAACATTTTGTGAAATTCTGTATCTTTAAAACTTGATTCAATCGCTTCTTTTATCTCTTCTGATGACACTCCTACTTGATTCgt   <  1:2438980/100‑2 (MQ=255)                                                              tttatctttaAAATTAAACATTTTGTGAAATTCTGTATCTTTAAAACTTGATTCAATCGCTTCTTTTATCTCTTCTGATGACACTCCTACTTGATTCGCa  <  1:920560/100‑1 (MQ=255)                                                                                   |                                                                               CAGGATAAAATTCTTTGAATATTATCTGTGGTGTGCTAACAACTGCGTCTTGAGCAAATTCTTTATCTTTAAAATTAAACATGTTGTGAAATTCACTATTTTTAAAACTTGATTCAATCGCTTCTTTTATCTCTTCTGATGACACTCCTACTTGATTCGCA  >  NC_007793/1579433‑1579593

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑