Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,579,416	T→A	V137V (GTA→GTT)	SAUSA300_RS07730 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,579,416	0	T	A	100.0%	50.4 / NA	19	V137V (GTA→GTT)	SAUSA300_RS07730	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (1/18);  total (1/18)

TCTTTCATGATTCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGCTGGTCGATAGAATATAAGTTGAAAGCTATGTCTATCGCTATTACAATTGCCAATTGGTCAGGATAAAATTCTTTGAATATTATCTGTGGTGTGCTAACAACTGCGTCTTGAGCAAATTCTTTATCTTTAAAAT  >  NC_007793/1579329‑1579507                                                                                        |                                                                                            tCTTTCGTGGTTCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGAATATAAGTTGTAAGCTATGTCTATCACTATAACAATTGcttgt                                                                                 <  1:4233818/100‑5 (MQ=255) ggcttCGTGGTTCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGAATATAAGTTGTAAGCTATGTCTATCACTATAACAATTGcttgt                                                                                 <  1:3104356/97‑5 (MQ=255) ccTTTCGTGGTTCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGAATATAAGTTGTAAGCTATGTCTATCACTATAACAATTGcttgt                                                                                 <  1:3510153/99‑5 (MQ=255)         ggttCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGAATATAAGTTGTAAGCTATGTCTATCACTATAACAATTGCTTGTTGGTCggg                                                                         <  1:487095/98‑1 (MQ=255)            tCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGAATATAAGTTGTAAGCTATGTCTATCACTATAACAATTGCTTGTTGGTCggggat                                                                      <  1:1151692/100‑4 (MQ=255)                                      tCAATGAGTTGGTCGATAGAATATAAGTTGTAAGCTATGTCTATCACTATAACAATTGCTTGTTGGTCGGGATAAAATTCTTTGAATATTATCTgtggtg                                            <  1:6186487/100‑1 (MQ=255)                                      tCAATGAGTTGGTCGATAGAATATAAGTTGTAAGCTATGTCTATCACTATAACAATTGCTTGTTGGTCGGGATAAAATTCTTTGAATATTATCTgtggtg                                            <  1:1467623/100‑1 (MQ=255)                                      tCAATGAGTTGGTCGATAGAATATAAGTTGTAAGCTATGTCTATCACTATAACAATTGCTTGTTGGTCGGGATAAAATTCTTTGAATATTATCTgtggtg                                            <  1:3404193/100‑1 (MQ=255)                                       cAATGAGTTGGTCGATAGAATATAAGTTGTAAGCTATGTCTATCACTATAACAATTGCTTGTTGGTCGGGATAAAATTCTTTGAATATTATCTgtggtgc                                           <  1:3122245/100‑2 (MQ=255)                                       cAATGAGTTGGTCGATAGAATATAAGTTGTAAGCTATGTCTATCACTATAACAATTGCTTGTTGGTCGGGATAAAATTCTTTGAATATTATCTGTGgtgt                                           <  1:339699/100‑1 (MQ=255)                                       cAATGAGTTGGTCGATAGAATATAAGTTGTAAGCTATGTCTATCACTATAACAATTGCTTGTTGGTCGGGATAAAATTCTTTGAATATTATCTGTGgtgt                                           <  1:4976794/100‑1 (MQ=255)                                       cAATGAGTTGGTCGATAGAATATAAGGTGTAAGCTATGTCTATCACTATAACAATTGCTTGTTGGTCGGGATAAAATTCTTTGAATATTATCTGTGgtgt                                           <  1:300032/100‑1 (MQ=255)                                       cAATGAGTTGGTCGATAGAATATAAGATGTAAGCTATGTCTATCACTATAACAATTGCTTGTTGGTCGGGATAAAATTCTTTGAATATTATCTGTGgtgt                                           <  1:2504517/100‑1 (MQ=255)                                         aTGAGTTGGTCGATAGAATATAAGTTGTAAGCTATGTCTATCACTATAACAATTGCTTGTTGGTCGGGATAAAATTCTTTGAATATTATCTGTGgtgtat                                         <  1:4380195/100‑3 (MQ=255)                                                         aaTATAAGTTGTAAGCTATGTCTATCACTATAACAATTGCTTGTTGGTCGGGATAAAATTCTTTGAATATTATCTGTGGTGTACTAACAACTGCGTCTTg                         <  1:3785607/100‑1 (MQ=255)                                                                 ttGTAAGCTATGTCTATCACTATAACAATTGCTTGTTGGTCGGGATAAAATTCTTTGAATATTATCTGTGGTGTACTAACAACTGCGTCTTGAGCAAAtt                 <  1:4064357/100‑1 (MQ=255)                                                                    taaGCTATGTCTATCACTATAACAATTGCTTGTTGGTCGGGATAAAATTCTTTGAATATTATCTGTGGTGTACTAACAACTGCGTCTTGAGCAAATtctt              <  1:2282958/99‑1 (MQ=255)                                                                     aaGCTATGTCTATCACTATAACAATTGCTTGTTGGTCGGGATAAAATTCTTTGAATATTATCTGTGGTGTACTAACAACTGCGTCTTGAGCAAATtcttt             <  1:1415878/100‑1 (MQ=255)                                                                                aTCACTATAACAATTGCTTGTTGGTCGGGTTAAAATTCTTTGAATATTATCTGTGGTGTACTAACAACTGCGTCTTGAGCAAATTCTTTATCTTTAAAAt  >  1:1553790/1‑100 (MQ=255)                                                                                        |                                                                                            TCTTTCATGATTCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGCTGGTCGATAGAATATAAGTTGAAAGCTATGTCTATCGCTATTACAATTGCCAATTGGTCAGGATAAAATTCTTTGAATATTATCTGTGGTGTGCTAACAACTGCGTCTTGAGCAAATTCTTTATCTTTAAAAT  >  NC_007793/1579329‑1579507

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑