Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,579,411	G→A	A139V (GCG→GTG)	SAUSA300_RS07730 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,579,411	0	G	A	100.0%	49.1 / NA	20	A139V (GCG→GTG)	SAUSA300_RS07730	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (1/19);  total (1/19)

CATCTTGTCTTTCATGATTCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGCTGGTCGATAGAATATAAGTTGAAAGCTATGTCTATCGCTATTACAATTGCCAATTGGTCAGGATAAAATTCTTTGAATATTATCTGTGGTGTGCTAACAACTGCGTCTTGAGCAAATTCTTTATCTTTAAAAT  >  NC_007793/1579322‑1579507                                                                                          |                                                                                                 cATCTTGTCTTTCGTGGTTCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGAATATAAGTTGTAAGCTATGTCTATCACTagctgggc                                                                                        <  1:3300474/100‑8 (MQ=255)        tCTTTCGTGGTTCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGAATATAAGTTGTAAGCTATGTCTATCACTATAACAATTGcttgt                                                                                 <  1:4233818/100‑5 (MQ=255)        ggcttCGTGGTTCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGAATATAAGTTGTAAGCTATGTCTATCACTATAACAATTGcttgt                                                                                 <  1:3104356/97‑5 (MQ=255)        ccTTTCGTGGTTCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGAATATAAGTTGTAAGCTATGTCTATCACTATAACAATTGcttgt                                                                                 <  1:3510153/99‑5 (MQ=255)                ggttCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGAATATAAGTTGTAAGCTATGTCTATCACTATAACAATTGCTTGTTGGTCggg                                                                         <  1:487095/98‑1 (MQ=255)                   tCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGAATATAAGTTGTAAGCTATGTCTATCACTATAACAATTGCTTGTTGGTCggggat                                                                      <  1:1151692/100‑4 (MQ=255)                                             tCAATGAGTTGGTCGATAGAATATAAGTTGTAAGCTATGTCTATCACTATAACAATTGCTTGTTGGTCGGGATAAAATTCTTTGAATATTATCTgtggtg                                            <  1:6186487/100‑1 (MQ=255)                                             tCAATGAGTTGGTCGATAGAATATAAGTTGTAAGCTATGTCTATCACTATAACAATTGCTTGTTGGTCGGGATAAAATTCTTTGAATATTATCTgtggtg                                            <  1:1467623/100‑1 (MQ=255)                                             tCAATGAGTTGGTCGATAGAATATAAGTTGTAAGCTATGTCTATCACTATAACAATTGCTTGTTGGTCGGGATAAAATTCTTTGAATATTATCTgtggtg                                            <  1:3404193/100‑1 (MQ=255)                                              cAATGAGTTGGTCGATAGAATATAAGTTGTAAGCTATGTCTATCACTATAACAATTGCTTGTTGGTCGGGATAAAATTCTTTGAATATTATCTgtggtgc                                           <  1:3122245/100‑2 (MQ=255)                                              cAATGAGTTGGTCGATAGAATATAAGTTGTAAGCTATGTCTATCACTATAACAATTGCTTGTTGGTCGGGATAAAATTCTTTGAATATTATCTGTGgtgt                                           <  1:339699/100‑1 (MQ=255)                                              cAATGAGTTGGTCGATAGAATATAAGTTGTAAGCTATGTCTATCACTATAACAATTGCTTGTTGGTCGGGATAAAATTCTTTGAATATTATCTGTGgtgt                                           <  1:4976794/100‑1 (MQ=255)                                              cAATGAGTTGGTCGATAGAATATAAGGTGTAAGCTATGTCTATCACTATAACAATTGCTTGTTGGTCGGGATAAAATTCTTTGAATATTATCTGTGgtgt                                           <  1:300032/100‑1 (MQ=255)                                              cAATGAGTTGGTCGATAGAATATAAGATGTAAGCTATGTCTATCACTATAACAATTGCTTGTTGGTCGGGATAAAATTCTTTGAATATTATCTGTGgtgt                                           <  1:2504517/100‑1 (MQ=255)                                                aTGAGTTGGTCGATAGAATATAAGTTGTAAGCTATGTCTATCACTATAACAATTGCTTGTTGGTCGGGATAAAATTCTTTGAATATTATCTGTGgtgtat                                         <  1:4380195/100‑3 (MQ=255)                                                                aaTATAAGTTGTAAGCTATGTCTATCACTATAACAATTGCTTGTTGGTCGGGATAAAATTCTTTGAATATTATCTGTGGTGTACTAACAACTGCGTCTTg                         <  1:3785607/100‑1 (MQ=255)                                                                        ttGTAAGCTATGTCTATCACTATAACAATTGCTTGTTGGTCGGGATAAAATTCTTTGAATATTATCTGTGGTGTACTAACAACTGCGTCTTGAGCAAAtt                 <  1:4064357/100‑1 (MQ=255)                                                                           taaGCTATGTCTATCACTATAACAATTGCTTGTTGGTCGGGATAAAATTCTTTGAATATTATCTGTGGTGTACTAACAACTGCGTCTTGAGCAAATtctt              <  1:2282958/99‑1 (MQ=255)                                                                            aaGCTATGTCTATCACTATAACAATTGCTTGTTGGTCGGGATAAAATTCTTTGAATATTATCTGTGGTGTACTAACAACTGCGTCTTGAGCAAATtcttt             <  1:1415878/100‑1 (MQ=255)                                                                                       aTCACTATAACAATTGCTTGTTGGTCGGGTTAAAATTCTTTGAATATTATCTGTGGTGTACTAACAACTGCGTCTTGAGCAAATTCTTTATCTTTAAAAt  >  1:1553790/1‑100 (MQ=255)                                                                                          |                                                                                                 CATCTTGTCTTTCATGATTCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGCTGGTCGATAGAATATAAGTTGAAAGCTATGTCTATCGCTATTACAATTGCCAATTGGTCAGGATAAAATTCTTTGAATATTATCTGTGGTGTGCTAACAACTGCGTCTTGAGCAAATTCTTTATCTTTAAAAT  >  NC_007793/1579322‑1579507

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑