Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,579,396	A→T	F144Y (TTC→TAC)	SAUSA300_RS07730 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,579,396	0	A	T	100.0%	50.2 / NA	20	F144Y (TTC→TAC)	SAUSA300_RS07730	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (1/19);  total (1/19)

TTCCTGCGTCTGCTGTTCC‑ATCTTGTCTTTCATGATTCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGCTGGTCGATAGAATATAAGTTGAAAGCTATGTCTATCGCTATTACAATTGCCAATTGGTCAGGATAAAATTCTTTGAATATTATCTGTGGTGTGCTAACAACTGCGTCTTGAGCAAATT  >  NC_007793/1579304‑1579492                                                                                              |                                                                                                 ttCCTGCGTCTGCTGTTCC‑ATCTTGTCTTTCGTGGTTCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGAATATAAGTTGTAAGCTAt                                                                                           >  1:3056748/1‑100 (MQ=255)   ccTGCGTCGGCTGTTCC‑ATCTTGTCTTTCGTGGTTCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGAATATAAGTTGTAAGCTATGt                                                                                         <  1:963394/100‑1 (MQ=255)   cagacGTGTGCTCTTCCGATC‑TGTCTTTCGTGGTTCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGAATATAAGTTGTAAGCTATGt                                                                                         <  1:3852389/96‑1 (MQ=255)                   c‑ATCTTGTCTTTCGTGGTTCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGAATATAAGTTGTAAGCTATGTCTATCACTagctgggc                                                                         <  1:3300474/100‑8 (MQ=255)                           tCTTTCGTGGTTCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGAATATAAGTTGTAAGCTATGTCTATCACTATAACAATTGcttgt                                                                  <  1:4233818/100‑5 (MQ=255)                           ggcttCGTGGTTCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGAATATAAGTTGTAAGCTATGTCTATCACTATAACAATTGcttgt                                                                  <  1:3104356/97‑5 (MQ=255)                           ccTTTCGTGGTTCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGAATATAAGTTGTAAGCTATGTCTATCACTATAACAATTGcttgt                                                                  <  1:3510153/99‑5 (MQ=255)                                   ggttCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGAATATAAGTTGTAAGCTATGTCTATCACTATAACAATTGCTTGTTGGTCggg                                                          <  1:487095/98‑1 (MQ=255)                                      tCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGTTGGTCGATAGAATATAAGTTGTAAGCTATGTCTATCACTATAACAATTGCTTGTTGGTCggggat                                                       <  1:1151692/100‑4 (MQ=255)                                                                tCAATGAGTTGGTCGATAGAATATAAGTTGTAAGCTATGTCTATCACTATAACAATTGCTTGTTGGTCGGGATAAAATTCTTTGAATATTATCTgtggtg                             <  1:3404193/100‑1 (MQ=255)                                                                tCAATGAGTTGGTCGATAGAATATAAGTTGTAAGCTATGTCTATCACTATAACAATTGCTTGTTGGTCGGGATAAAATTCTTTGAATATTATCTgtggtg                             <  1:6186487/100‑1 (MQ=255)                                                                tCAATGAGTTGGTCGATAGAATATAAGTTGTAAGCTATGTCTATCACTATAACAATTGCTTGTTGGTCGGGATAAAATTCTTTGAATATTATCTgtggtg                             <  1:1467623/100‑1 (MQ=255)                                                                 cAATGAGTTGGTCGATAGAATATAAGTTGTAAGCTATGTCTATCACTATAACAATTGCTTGTTGGTCGGGATAAAATTCTTTGAATATTATCTgtggtgc                            <  1:3122245/100‑2 (MQ=255)                                                                 cAATGAGTTGGTCGATAGAATATAAGTTGTAAGCTATGTCTATCACTATAACAATTGCTTGTTGGTCGGGATAAAATTCTTTGAATATTATCTGTGgtgt                            <  1:339699/100‑1 (MQ=255)                                                                 cAATGAGTTGGTCGATAGAATATAAGTTGTAAGCTATGTCTATCACTATAACAATTGCTTGTTGGTCGGGATAAAATTCTTTGAATATTATCTGTGgtgt                            <  1:4976794/100‑1 (MQ=255)                                                                 cAATGAGTTGGTCGATAGAATATAAGGTGTAAGCTATGTCTATCACTATAACAATTGCTTGTTGGTCGGGATAAAATTCTTTGAATATTATCTGTGgtgt                            <  1:300032/100‑1 (MQ=255)                                                                 cAATGAGTTGGTCGATAGAATATAAGATGTAAGCTATGTCTATCACTATAACAATTGCTTGTTGGTCGGGATAAAATTCTTTGAATATTATCTGTGgtgt                            <  1:2504517/100‑1 (MQ=255)                                                                   aTGAGTTGGTCGATAGAATATAAGTTGTAAGCTATGTCTATCACTATAACAATTGCTTGTTGGTCGGGATAAAATTCTTTGAATATTATCTGTGgtgtat                          <  1:4380195/100‑3 (MQ=255)                                                                                   aaTATAAGTTGTAAGCTATGTCTATCACTATAACAATTGCTTGTTGGTCGGGATAAAATTCTTTGAATATTATCTGTGGTGTACTAACAACTGCGTCTTg          <  1:3785607/100‑1 (MQ=255)                                                                                           ttGTAAGCTATGTCTATCACTATAACAATTGCTTGTTGGTCGGGATAAAATTCTTTGAATATTATCTGTGGTGTACTAACAACTGCGTCTTGAGCAAAtt  <  1:4064357/100‑1 (MQ=255)                                                                                              |                                                                                                 TTCCTGCGTCTGCTGTTCC‑ATCTTGTCTTTCATGATTCCTTTTCATTTTCTTTTTGTATGCGTCAATGAGCTGGTCGATAGAATATAAGTTGAAAGCTATGTCTATCGCTATTACAATTGCCAATTGGTCAGGATAAAATTCTTTGAATATTATCTGTGGTGTGCTAACAACTGCGTCTTGAGCAAATT  >  NC_007793/1579304‑1579492

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑