| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_007793 | 1,579,043 | T→A | E3V (GAG→GTG) | SAUSA300_RS07720 ← | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_007793 | 1,579,043 | 0 | T | A | 92.9% | 24.1 / ‑4.6 | 14 | E3V (GAG→GTG) | SAUSA300_RS07720 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base A (0/13); minor base G (0/1); total (0/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.11e-01 | |||||||||||
AATCACTAGATTCATAACAAGATTAATATAATCGTCAGAAGCTAGATCTGTTGTTGTGTAATAAACTCTATCTGAAATAGTTTTAATTTTAACCTCCGTCATTTCCCACACTCCCTTATATTTTCAAATAACTGACCCACTTTAATAACTGCATCTCTTTTAACTTGTGCCTCGTATT > NC_007793/1578949‑1579126 | aaacACTAGGTTCATAACAAGATTAATAGAATCGTCAGAAGCTAAATCTGTTGTTGTGTAATAAACTCTATCTGAAATAGTTTTAATTTTAACCACCgcc < 1:6269197/97‑3 (MQ=255) acACTAGGTTCATAACAAGATTAATATAATCGTCAGAAGCTAAATCTGTTGTTGTGTAATAAACTCTATCTGAAATAGTTTTAATTTTAACCACCGTCAt < 1:2691460/99‑1 (MQ=255) cACTAGGTTCATAACAAGATTAATATAATCGTCAGAAGCTAAATCTTTTGTTGTGTAACAAACTCTATCTGAAATAGTTTTAATTTTAACCACCGTCAtg < 1:805665/100‑2 (MQ=255) aCTAGGTTCATAACAAGATTAATATAATCGTCAGAAGCTAAATCTGTTGTTGTGTAATAAACTCTATCTGAAATAGTTTTAATTTTAACCACCATCAttg < 1:5856423/100‑2 (MQ=255) gttCATAACAAGATTAATATAATCGTCAGAAGCTAAATCTGTTGTTGTGTAATAAACTCTATCTGAAATAGTTTTAATTTTAACCACCGTCATTTCCcac < 1:174955/99‑1 (MQ=255) gttCATAACAAGATTAATATAATCGTCAGAAGCTAAATCTGTTGTTGTGTAATAAACTCTATCTGAAATAGTTTTAATTTTAACCACCGTCATTTCCcac < 1:360057/99‑1 (MQ=255) tCATAACAAGATTAATATAATCGTCAGAAGCTAAATCTGTTGTTGTGTAATAAACTCTATCTGAAATAGTTTTAATTTTAACCACCGTCATTTCCcacag < 1:4161458/100‑2 (MQ=255) aaGATTAATATAATCGTCAGAAGCTAAATCTGTTGTTGTGTAATAAACTCTATCTGAAATAGTTTTAATTTTAACCACCGTCATTTCCCACACTCCCtgt < 1:4748137/100‑3 (MQ=255) tataATCGTCAGAAGCTAAATCTGTTGTTGTGTAATAAACTCTATCTGAAATAGTTTTAATTTTAACCACCGTCATTTCCCACACTCCCTTATATTTTCa < 1:2903441/100‑1 (MQ=255) ccgATCTGTTGTTGTGTAATAAACTCTATCTGAAATAGTTTTAATTTTAACCACCGTCATTTCCCACACTCCCTTATATTTTCAAACAACTGACTCACta < 1:4121431/98‑2 (MQ=37) aaTCTGTTGTTGTGTAATAAACTCTATCTGAAATAGTTTTAATTTTAACCACCGTCATTTCCCACACTCCCTTATATTTTCAAACAACTGACTCACTTta < 1:2626933/99‑1 (MQ=255) gaTCTAAATTAGTTTAAATTTAAGCCGCCGTCATTTCCCACACTCCCTTATATTTTCAAACAACTGACTCACTTTAATAATTGCATCCCTTTTAACTtgt < 1:2037209/99‑1 (MQ=11) gAAATAGTTTTAATTTTAACCACCGTCATTTCCCACACTCCCTTATATTTTCAAACAACTGACTCACTTTAATAATTGCATCCCTTTTAACTtgccacat < 1:5426100/100‑7 (MQ=17) aGTTTTAATTTTAACCACCGTCATTTCCTACACTCCCTTATATTTTCAAACAACTGACTCACTTTAATAATTGCATCCCTTTTAACTTGCGCCTCGTact < 1:5664810/100‑3 (MQ=17) | AATCACTAGATTCATAACAAGATTAATATAATCGTCAGAAGCTAGATCTGTTGTTGTGTAATAAACTCTATCTGAAATAGTTTTAATTTTAACCTCCGTCATTTCCCACACTCCCTTATATTTTCAAATAACTGACCCACTTTAATAACTGCATCTCTTTTAACTTGTGCCTCGTATT > NC_007793/1578949‑1579126 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |