Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,578,951	T→A	I34F (ATT→TTT)	SAUSA300_RS07720 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,578,951	0	T	A	100.0%	37.6 / NA	15	I34F (ATT→TTT)	SAUSA300_RS07720	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (4/11);  total (4/11)

GTTTGCCTCCTTAATAAATGTAAATGATTCAATCTCATCTCTTTTAACCCATACTTCATTGTTGAACACATCTTTGACCGGAAGAAAATCCTCAATCACTAGATTCATAACAAGATTAATATAATCGTCAGAAGCTAGATCTGTTGTTGTGTAATAAACTCTATCTGAAATAG  >  NC_007793/1578856‑1579028                                                                                                |                                                                              gTTTGCCTCCTTAATAAATGTAAATGATTCAATCTCATCTCTTTTAACCCATACTTCATTGTTGAACACATCTTTGACCGGAAGAAAATCCTCAAACACt                                                                           <  1:6262122/100‑1 (MQ=255)       ctcctTAATAAATGTAAATGATTCAATCTCATCTCTTTTAACCCATACTTCATTGTTGAACACATCTTTGACCGGAAGAAAATCCTCAAACACTAggtgt                                                                     <  1:3833079/100‑5 (MQ=255)       ctcctTAATAAATGTAAATGATTCAATCTCATCTCTTTTAACCCATACTTCATTGTTGAACACATCTTTGACCGGAAGAAAATCCTCAAACACTAGGTTc                                                                     <  1:987903/100‑1 (MQ=255)        tcctTAATAAATGTAAATGATTCAATCTCATCTCTTTTAACCCATACTTCATTGTTGAACACATCTTTGACCGGAAGAAAATCCTCAAACACTAGGTTCa                                                                    <  1:184235/100‑1 (MQ=255)          ctTAATAAATGTAAATGATTCAATCTCATCTCTTTTAACCCATACTTCATTGTTGAACACATCTTTGACCGGAAGAAAATCCTCAAACACTAGGTTCAtg                                                                  <  1:5106245/100‑2 (MQ=255)                     taaatgATTCAATCTCATCTCTTTTAACCCATACTTCATTGTTGAACACATCTTTGACCGGAAGAAAATCCTCAAACACTAGGTTCATAACAAGATTAat                                                       <  1:2258866/100‑1 (MQ=255)                     taaatgATTCAATCTCATCTCTTTTAACCCATACTTCATTGTTGAACACATCTTTGACCGGAAGAAAATCCTCAAACACTAGGTTCATAACAAGATTAat                                                       <  1:6588081/100‑1 (MQ=255)                              cAATCTCATCTCTTTTAACCCATACTTCATTGTTGAACACATCTTTGACCGGAAGAAAATCCTCAAACACTAGGTTCATAACAAGATTAATATAATCGTc                                              <  1:247867/100‑1 (MQ=255)                              cAATCTCATCTCTTTTAACCCATACTTCATTGTTGAACACATCTTTGACCGGAAGAAAATCCTCAAACACTAGGTTCATAACAAGATTAATATAATCGTc                                              <  1:2723360/100‑1 (MQ=255)                                  ctcatctcTTTTAACCCATACTTCATTGTTGAACACATCTTTGACCGGAAGAAAATCCTCAAACACTAGGTTCATAACAAGATTAATATAATCGTCAGat                                          <  1:3304512/100‑2 (MQ=255)                                    catctcTTTTAACTCATACTTCATTGTTGAACACATCTTTGACCGGAAGAAAATCCTCAAACACTAGGTTCATAACAAGATTAATATAATCGTCAGAAGc                                        >  1:5126384/1‑100 (MQ=255)                                    catctcTTTTAACCCATACTTCATTGTTGAACACATCTTTGACCGGAAGAAAATCCTCAAACACTAGGTTCATAACAAGATTAATATAATCGTCAGAAGc                                        >  1:5170450/1‑100 (MQ=255)                                           tttAACCCATACTTCATTGTTGAACACATCTTTGACCGGAAGAAAATCCTCAAACACTAGGTTCATAACAAGATTAATATAATCGTCAGAAGCTAAATCt                                 >  1:6387324/1‑100 (MQ=255)                                                      cTTCATTGTTGAACACATCTTTGACCGGAAGAAAATCCTCAAACACTAGGTTCATAACAAGATTAATATAATCGTCAGAAGCTAAATCTGTTGTTGTGta                      <  1:6564458/100‑1 (MQ=255)                                                                          ttGACCGGAAGAAAATCCTCAAACACTAGGTTCATAACAAGATTAATATAATCGTCAGAAGCTAAATCTGTTGTTGTGTAATAAACTCTATCTGAAATAg  >  1:4552620/1‑100 (MQ=255)                                                                                                |                                                                              GTTTGCCTCCTTAATAAATGTAAATGATTCAATCTCATCTCTTTTAACCCATACTTCATTGTTGAACACATCTTTGACCGGAAGAAAATCCTCAATCACTAGATTCATAACAAGATTAATATAATCGTCAGAAGCTAGATCTGTTGTTGTGTAATAAACTCTATCTGAAATAG  >  NC_007793/1578856‑1579028

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑