Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,578,853	A→G	M1T (ATG→ACG) †	SAUSA300_RS07715 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,578,853	0	A	G	100.0%	68.8 / NA	28	M1T (ATG→ACG) †	SAUSA300_RS07715	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/19);  total (9/19)

TCTTCAATCTTAGCCTTTTCTTCAGGTGCTTTATTTTTATATTTTTTGATTATTTCAGTGTACTTTTTCGGGAATTTCATTTTAGGTATGTTAATCATCGTTTGCCTCCTTAATAAATGTAAATGATTCAATCTCATCTCTTTTAACCCATACTTCATTGTTGAACACATCTT  >  NC_007793/1578757‑1578929                                                                                                 |                                                                             cttccgaTCTTAGCTTTTTCTTCAGGTGTTTTATTTTTATATTTCTTGATTATTTCAGTGTACTTTTTCGGGAATTTCATTTTAGGTATGTTAatcgtca                                                                           <  1:602314/94‑2 (MQ=255)   tccgaTCTTAGCTCTTTCTTCAGGTGTTTTATCTTTATATTTCTTGATTATTTCAGTGTACTTTTTCGGGAATTTCATTTTAGGTATGTTAATCGTCGtt                                                                         <  1:331796/96‑1 (MQ=255)    tCAATCTTAGCTTTTTCTTCAGGTGTTTTATTTTTATATTTCTTGATTATTTCAGTGTACTTTTTCGGGAATTTCATTTTAGGTATGTTAATCGTCGttt                                                                        <  1:769779/100‑1 (MQ=255)        ctatAGCTTTTTCTTCAGGTGTTTTATTTTTATATTTCTTGATTATTTCAGTGTACTTTTTCGGGAATTTCATTTTAGGTATGTTAATCGTCGTTTGcct                                                                    <  1:5756763/97‑1 (MQ=255)        ccTTAGCTCTTTCTTCAGGTGTTTTATTTTTATATTGCTTGATTAATTCAGTGTACTTTTTCGGGAATGTCATTTTATGTATGTTAATCGTCGTTTGcct                                                                    <  1:3136575/99‑1 (MQ=255)          ctAGCTTTTTCTTCAGGTGTTTTATTTTTATATTTCTTGATTATTTCAGTGTACTTTTTCGGGAATTTCATTTTAGGTATGTTAATCGTCGTTTGcctcc                                                                  >  1:2253350/2‑100 (MQ=255)           tggcgttttCTTCAGGTGTTTTATTTTTATATTTCTTGATTATTTCAGTGTACTTTTTCGGGAATTTCATTTTAGGTATGTTAATCGTCGTTTGcctcct                                                                 >  1:5618552/6‑100 (MQ=255)           tAGCTTTTTCTTCAGGTGTTTTATTTTTATATTTCTTGATTATTTCAGTGTACTTTTTCGGGAATTTCATTTTAGGTATGTTAATCGTCGTTTGcctcct                                                                 >  1:6168786/1‑100 (MQ=255)           tAGCTTTTTCTTCAGGTGTTTTATTTTTATATTTATTGATTATTTCAGTGTACTTTTTCGGGAATTTCATTTTAGGTATGTTAATCGTCGTTTGcctcct                                                                 >  1:4335275/1‑100 (MQ=255)           acgctttttCTTCAGGTGTTTTATTTTTATATTTCTTGATTATTTCAGTGTACTTTTTCGGGAATTTCATTTTAGGTATGTTAATCGTCGTTTGcctcct                                                                 >  1:6395019/6‑100 (MQ=255)            ggctttttCTTCAGGTGTTTTATTTTTATATTTCTTGATTATTTCAGTGTACTTTTTCGGGAATTTCATTTTAGGTATGTTAATCGTCGTTTGCCTCCtt                                                                <  1:1892265/96‑1 (MQ=255)             gctttttCTTCAGGTGTTTTATTTTTATATTTCTTGATTATTTCAGTGTACTTTTTCGGGAATTTCATTTTAGGTATGTTAATCGTCGTTTGCCTCCTta                                                               <  1:5063034/97‑1 (MQ=255)                     ctcAGGTGTTTTATTTTTATATTTCTTGATTATTTCAGTGTACTTTTTCGGGAATTTCATTTTAGGTATGTTAATCGTCGTTTGCCTCCTTAAtaaatgt                                                       >  1:5422518/2‑100 (MQ=255)                      tttggTGTTTTATTTTTATATTTCTTGATTATTTCAGTGTACTTTTTCGGGAATTTCATCTTAGGTATGTTAATCGTCGTTTGCCTCCTTAAtaaatgta                                                      >  1:6271426/4‑100 (MQ=255)                      tcAGGTGTTTTATTTTTATATTTCTTGATTATTTCAGTGTACTTTTTCGGGAATTTCATTTTAGGTATGTTAATCGTCGTTTGCCTCCTTAAtaaatgta                                                      <  1:444880/100‑1 (MQ=255)                                tATTTTTATATTTCTTGATTATTTCAGTGTACTTTTTCGGGAATTTCATTTTAGGTATGTTAATCGTCGTTTGCCTCCTTAATAAATGTAAATGATTCac                                            <  1:6180366/100‑2 (MQ=255)                                          tttcttGATTATTTCAGTGTACTTTTTCGGGAATTTCATTTTAGGTATGTTAATCGTCGTTTGCCTCCTTAATAAATGTAAATGATTCAatctcatctcg                                  <  1:3764962/100‑2 (MQ=255)                                               tGATTATTTCAGTGTACTTTTTCGGGAATTTCGTTTTAGGTATGTTAATCGTCGTTTGCCTCCTTAATAAATGCAAATGATTCAATCTCATCTCTTTTaa                             >  1:5894823/1‑100 (MQ=255)                                                    attTCAGTGTACTTTTTCGGGAATTTCATTTTAGGTATGTTAATCGTCGTTTGCCTCCTTAATAAATGTAAATGATTCAATCTCATCTCTTTTAACCCag                        <  1:217842/100‑2 (MQ=255)                                                    attTCAGTGTACTTTTTCGGGAATTTCATTTTAGGTATGTTAATCGTCGTTTGCCTCCTTAATAAATGTAAATGATTCAATCTCATCTCTTTTAACCCac                        <  1:720242/100‑2 (MQ=255)                                                     tttCAGTGTACTTTTTCGGGAATTTCATTTTAGGTATGTTAATCGTCGTTTGCCTCCTTAATAAATGTAAATGATTCAATCTCATCTCTTTTAACCCAtt                       <  1:754532/100‑2 (MQ=255)                                                     tttCAGAGTACTTTTTCGGGAATTTCATTTTAGGTATGTTAATCGTCGTTTGCCTCCTTAATAAATGTAAATGATTCAATCTCATCTCTTTTAACCCAtg                       <  1:4419934/100‑2 (MQ=255)                                                      ttCAGTGTACTTTTTCGGGAATTTCATTTTAGGTATGTTAATCGTCGTTTGCCTCCTTAATAAATGTAAATGATTCAATCTCATCTCTTTTAACCCATag                      <  1:1393879/100‑2 (MQ=255)                                                       tCAGTGTACTTTTTCGGGAATTTCATTTTAGGTATGTTAATCGTCGTTTGCCTCCTTAATAAATGTAAATGATTCAATCTCATCTCTTTTAACCCATACt                     <  1:903617/100‑1 (MQ=255)                                                        cAGTGTACTTTTTCGGGAATTTCATTTTAGGTATGTTAATCGTCGTTTGCCTCCTTAATAAATGTAAATGATTCAATCTCATCTCTTTTAACCCATACtt                    <  1:5831967/100‑1 (MQ=255)                                                           tgtACTTTTTCGGGAATTTCATTTTAGGTATGTTAATCGTCGTTTGCCTCCTTAATAAATGTAAATGATTCAATCTCATCTCTTTTAACCCATACTTCAt                 >  1:6157818/1‑100 (MQ=255)                                                             tACTTTTGCGGGAATTTCATTTTAAGTATGTTAATCGTCGTTTGCCTCCTTAATAAATGTAAATGATTCAATCTCATCTCTTTTAACCCATACTTCAttg               <  1:711861/100‑1 (MQ=255)                                                                tttttCGGGAATTTCATTTTAGGTATGTTAATCGTCGTTTGCCTCCTTAATAAATGTAAATGATTCAATCTCATCTCTTTTAACCCATACTTCAttgttg            <  1:5377224/100‑1 (MQ=255)                                                                          aTTTCATTTTAGGTATGTTAATCGTCGTTTGCCTCCTTAATAAATGTAAATGATTCAGTCGCATCTCTTTTAACCCATACTTCATTGTTGAACACATCtt  <  1:507853/100‑1 (MQ=255)                                                                                                 |                                                                             TCTTCAATCTTAGCCTTTTCTTCAGGTGCTTTATTTTTATATTTTTTGATTATTTCAGTGTACTTTTTCGGGAATTTCATTTTAGGTATGTTAATCATCGTTTGCCTCCTTAATAAATGTAAATGATTCAATCTCATCTCTTTTAACCCATACTTCATTGTTGAACACATCTT  >  NC_007793/1578757‑1578929

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑