Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,578,785	C→T	A24T (GCA→ACA)	SAUSA300_RS07715 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,578,785	0	C	T	100.0%	39.1 / NA	16	A24T (GCA→ACA)	SAUSA300_RS07715	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/8);  total (8/8)

TTATCTTTAATTTCTTTAATAAAATCATCTTCAATCTTAGCCTTTTCTTCAGGTGCTTTATTTTTATATTTTTTGATTATTTCAGTGTACTTTTTCGGGAATTTCATTTTAGGTATGTTAATCATCGTTTGCCTCCTTAATAAATGTA  >  NC_007793/1578730‑1578877                                                        |                                                                                             ttatcattaATTTCTTTAATGAAATCATCTTCAATCTTAGCTTTTTCTTCAGGTGTTTTATTTTTATATTTCTTGATTATTTCAGTGTACTTTTTCGGGa                                                  >  1:1740296/1‑100 (MQ=255)                            cttccgaTCTTAGCTTTTTCTTCAGGTGTTTTATTTTTATATTTCTTGATTATTTCAGTGTACTTTTTCGGGAATTTCATTTTAGGTATGTTAatcgtca                       <  1:602314/94‑2 (MQ=255)                              tccgaTCTTAGCTCTTTCTTCAGGTGTTTTATCTTTATATTTCTTGATTATTTCAGTGTACTTTTTCGGGAATTTCATTTTAGGTATGTTAATCGTCGtt                     <  1:331796/96‑1 (MQ=255)                               tCAATCTTAGCTTTTTCTTCAGGTGTTTTATTTTTATATTTCTTGATTATTTCAGTGTACTTTTTCGGGAATTTCATTTTAGGTATGTTAATCGTCGttt                    <  1:769779/100‑1 (MQ=255)                                   ctatAGCTTTTTCTTCAGGTGTTTTATTTTTATATTTCTTGATTATTTCAGTGTACTTTTTCGGGAATTTCATTTTAGGTATGTTAATCGTCGTTTGcct                <  1:5756763/97‑1 (MQ=255)                                   ccTTAGCTCTTTCTTCAGGTGTTTTATTTTTATATTGCTTGATTAATTCAGTGTACTTTTTCGGGAATGTCATTTTATGTATGTTAATCGTCGTTTGcct                <  1:3136575/99‑1 (MQ=255)                                     ctAGCTTTTTCTTCAGGTGTTTTATTTTTATATTTCTTGATTATTTCAGTGTACTTTTTCGGGAATTTCATTTTAGGTATGTTAATCGTCGTTTGcctcc              >  1:2253350/2‑100 (MQ=255)                                      tggcgttttCTTCAGGTGTTTTATTTTTATATTTCTTGATTATTTCAGTGTACTTTTTCGGGAATTTCATTTTAGGTATGTTAATCGTCGTTTGcctcct             >  1:5618552/6‑100 (MQ=255)                                      tAGCTTTTTCTTCAGGTGTTTTATTTTTATATTTCTTGATTATTTCAGTGTACTTTTTCGGGAATTTCATTTTAGGTATGTTAATCGTCGTTTGcctcct             >  1:6168786/1‑100 (MQ=255)                                      tAGCTTTTTCTTCAGGTGTTTTATTTTTATATTTATTGATTATTTCAGTGTACTTTTTCGGGAATTTCATTTTAGGTATGTTAATCGTCGTTTGcctcct             >  1:4335275/1‑100 (MQ=255)                                      acgctttttCTTCAGGTGTTTTATTTTTATATTTCTTGATTATTTCAGTGTACTTTTTCGGGAATTTCATTTTAGGTATGTTAATCGTCGTTTGcctcct             >  1:6395019/6‑100 (MQ=255)                                       ggctttttCTTCAGGTGTTTTATTTTTATATTTCTTGATTATTTCAGTGTACTTTTTCGGGAATTTCATTTTAGGTATGTTAATCGTCGTTTGCCTCCtt            <  1:1892265/96‑1 (MQ=255)                                        gctttttCTTCAGGTGTTTTATTTTTATATTTCTTGATTATTTCAGTGTACTTTTTCGGGAATTTCATTTTAGGTATGTTAATCGTCGTTTGCCTCCTta           <  1:5063034/97‑1 (MQ=255)                                                ctcAGGTGTTTTATTTTTATATTTCTTGATTATTTCAGTGTACTTTTTCGGGAATTTCATTTTAGGTATGTTAATCGTCGTTTGCCTCCTTAAtaaatgt   >  1:5422518/2‑100 (MQ=255)                                                 tttggTGTTTTATTTTTATATTTCTTGATTATTTCAGTGTACTTTTTCGGGAATTTCATCTTAGGTATGTTAATCGTCGTTTGCCTCCTTAAtaaatgta  >  1:6271426/4‑100 (MQ=255)                                                 tcAGGTGTTTTATTTTTATATTTCTTGATTATTTCAGTGTACTTTTTCGGGAATTTCATTTTAGGTATGTTAATCGTCGTTTGCCTCCTTAAtaaatgta  <  1:444880/100‑1 (MQ=255)                                                        |                                                                                             TTATCTTTAATTTCTTTAATAAAATCATCTTCAATCTTAGCCTTTTCTTCAGGTGCTTTATTTTTATATTTTTTGATTATTTCAGTGTACTTTTTCGGGAATTTCATTTTAGGTATGTTAATCATCGTTTGCCTCCTTAATAAATGTA  >  NC_007793/1578730‑1578877

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑