Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,578,771	C→T	K28K (AAG→AAA)	SAUSA300_RS07715 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,578,771	0	C	T	100.0%	20.7 / NA	10	K28K (AAG→AAA)	SAUSA300_RS07715	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/6);  total (4/6)

TTCATTCATATTAGCCATCGTAGGACTGTAAAATTCACTGTCTTTATCTTTAATTTCTTTAATAAAATCATCTTCAATCTTAGCCTTTTCTTCAGGTGCTTTATTTTTATATTTTTTGATTATTTCAGTGTACTTTTTCGGGAATTTCATTTTAGGTATGTTAATCATCGTTTGCCTCCT  >  NC_007793/1578687‑1578866                                                                                     |                                                                                                ttcattcaTATTAGCCATCATAGGACTGTAAAATTCACTGTCTTTATCATTAATTTCTTTAATGAAATCATCTTCAATCTTAGCTTTTTCTTCAGGTgtt                                                                                  <  1:5168944/100‑3 (MQ=255)                                            ttatcattaATTTCTTTAATGAAATCATCTTCAATCTTAGCTTTTTCTTCAGGTGTTTTATTTTTATATTTCTTGATTATTTCAGTGTACTTTTTCGGGa                                       >  1:1740296/1‑100 (MQ=255)                                                                       cttccgaTCTTAGCTTTTTCTTCAGGTGTTTTATTTTTATATTTCTTGATTATTTCAGTGTACTTTTTCGGGAATTTCATTTTAGGTATGTTAatcgtca            <  1:602314/94‑2 (MQ=255)                                                                         tccgaTCTTAGCTCTTTCTTCAGGTGTTTTATCTTTATATTTCTTGATTATTTCAGTGTACTTTTTCGGGAATTTCATTTTAGGTATGTTAATCGTCGtt          <  1:331796/96‑1 (MQ=255)                                                                          tCAATCTTAGCTTTTTCTTCAGGTGTTTTATTTTTATATTTCTTGATTATTTCAGTGTACTTTTTCGGGAATTTCATTTTAGGTATGTTAATCGTCGttt         <  1:769779/100‑1 (MQ=255)                                                                              ctatAGCTTTTTCTTCAGGTGTTTTATTTTTATATTTCTTGATTATTTCAGTGTACTTTTTCGGGAATTTCATTTTAGGTATGTTAATCGTCGTTTGcct     <  1:5756763/97‑1 (MQ=255)                                                                              ccTTAGCTCTTTCTTCAGGTGTTTTATTTTTATATTGCTTGATTAATTCAGTGTACTTTTTCGGGAATGTCATTTTATGTATGTTAATCGTCGTTTGcct     <  1:3136575/99‑1 (MQ=255)                                                                                ctAGCTTTTTCTTCAGGTGTTTTATTTTTATATTTCTTGATTATTTCAGTGTACTTTTTCGGGAATTTCATTTTAGGTATGTTAATCGTCGTTTGcctcc   >  1:2253350/2‑100 (MQ=255)                                                                                 tAGCTTTTTCTTCAGGTGTTTTATTTTTATATTTCTTGATTATTTCAGTGTACTTTTTCGGGAATTTCATTTTAGGTATGTTAATCGTCGTTTGcctcct  >  1:6168786/1‑100 (MQ=255)                                                                                 tAGCTTTTTCTTCAGGTGTTTTATTTTTATATTTATTGATTATTTCAGTGTACTTTTTCGGGAATTTCATTTTAGGTATGTTAATCGTCGTTTGcctcct  >  1:4335275/1‑100 (MQ=255)                                                                                     |                                                                                                TTCATTCATATTAGCCATCGTAGGACTGTAAAATTCACTGTCTTTATCTTTAATTTCTTTAATAAAATCATCTTCAATCTTAGCCTTTTCTTCAGGTGCTTTATTTTTATATTTTTTGATTATTTCAGTGTACTTTTTCGGGAATTTCATTTTAGGTATGTTAATCATCGTTTGCCTCCT  >  NC_007793/1578687‑1578866

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑