Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,578,686	A→G	Y57H (TAT→CAT)	SAUSA300_RS07715 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,578,686	0	A	G	100.0%	11.6 / NA	7	Y57H (TAT→CAT)	SAUSA300_RS07715	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (0/7);  total (0/7)

ATCCATATTTTTAAGTTTTTTAATCATCATTGTCATCTCCAGTATCAATTAAACTAGGCATCATTCTTAACATAGCCCTTAATTCATATTCATTCATATTAGCCATCGTAGGACTGTAAAATTCACTGTCTTTATCTTTAATTTCTTTAATAAAATCATCTTCAATCTTAGCCT  >  NC_007793/1578599‑1578772                                                                                        |                                                                                       atccatATTTTTAAGTTTTTTAATCATCATTGTCATCTCCAGTATCAATTAAACTAGGCATCATTCTTAACATAGCCCTTAATTCATGTTCATTCATAtt                                                                            <  1:684331/100‑1 (MQ=255)     atatTTTTAAGTTTTTTAATCATCATTGTCATCTCCAGTATCAATTAAACTAGGCATCATTCTTAACATAGCCCTTAATTCATGTTCATTCATATTAGcc                                                                        <  1:1448204/100‑1 (MQ=255)     atatTTTTAAGTTTTTTAATCATCATTGTCATCTCCAGTATCAATTAAACTAGGCATCATTCTTAACATAGCCCTTAATTCATGTTCATTCATATTAGcc                                                                        <  1:2234828/100‑1 (MQ=255)          tttAAGTTTTTTAATCATCATTGTCATCTCCAGTATCAATTAAACTAGGCATCATTCTTAACATAGCCCTTAATTCATGTTCATTCATATTAGCCATcat                                                                   <  1:502141/100‑3 (MQ=255)          tttAAGTTTTTTAATCATCATTGTCATCTCCAGTATCAATTAAACTAGGCATCATTCTTAACATAGCCCTTAATTCATGTTCATTCATATTAGCCATcat                                                                   <  1:5648829/100‑3 (MQ=255)                                                                        ctggCCCTTAATTCATGTTCATTCATATTAGCCATCATAGGATTGTAAAATTCACTGTCTTTATCATTAATTTCTTTAATGAAATCATCTTCAATCTTAg     <  1:6131967/97‑1 (MQ=255)                                                                           gCCCATAATTCATGTTCATTCATATTAGCCATCATAGGACTGTAAAATTCACTGTCTTTAACATTAATTTCTTTAATGAAATCATCTTCAATCTTAGctt  <  1:1808081/100‑3 (MQ=255)                                                                                        |                                                                                       ATCCATATTTTTAAGTTTTTTAATCATCATTGTCATCTCCAGTATCAATTAAACTAGGCATCATTCTTAACATAGCCCTTAATTCATATTCATTCATATTAGCCATCGTAGGACTGTAAAATTCACTGTCTTTATCTTTAATTTCTTTAATAAAATCATCTTCAATCTTAGCCT  >  NC_007793/1578599‑1578772

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑