Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	240,358	T→A	N358K (AAT→AAA)	SAUSA300_RS01070 →	peptide ABC transporter substrate‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	240,358	0	T	A	100.0%	33.7 / NA	15	N358K (AAT→AAA)	SAUSA300_RS01070	peptide ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (14/1);  total (14/1)

AAGATGAGGACATCGATCAGTTGCGTAAAGAGAAAGACAAGGACGATAAATCAATCTATGATAAGTTGATTAAAGCTTATAACGTCAATTCGTTAACGACAGATGGTCAAAAAGTAAATAATAAAGACGGTATTTATCAAATTGTTAAAATTACGACAGATCAA  >  NC_007793/240270‑240433                                                                                         |                                                                            aaGATGAGGACATCGATCAGTTGCGTAAAGAGAAAGACAAGGACGATAAATCAATCTATGATAAGTTGATTAAAGCTTATAACGTCAAATCGTTAACGAc                                                                  >  1:1962324/1‑100 (MQ=255)                  cAGTTGCGTAAAGAGAAAGACAAGGACGATAAATCAATCTATGATAAGTTGATTAAAGCTTATAACGTCAAATCGTTAACGACAGATGGTCAAAAAGTaa                                                 >  1:2645247/1‑100 (MQ=255)                  cAGTTGCGTAAAGAGAAAGACAAGGACGATAAATCAATCTATGATAAGTTGATTAAAGCTTATAACGTCAAATCGTTAACGACAGATGGTCAAAAAGTaa                                                 >  1:3330799/1‑100 (MQ=255)                  cAGTTGCGTAAAGAGAAAGACAAGGACGATAAATCAATCTATGATAAGTTGATTAAAGCTTATAACGTCAAATCGTTAACGACAGATGGTCAAAAAGTaa                                                 >  1:6480806/1‑100 (MQ=255)                    gTTGCGTAAAGAGGAAGACAAGGACGATAAATCAATCTATGATAAGTTGATTAAAGCTTATAACGTCAAATCGTTAACGACAGATGGTCAAAAAGTAaat                                               >  1:1860858/1‑100 (MQ=255)                                   aGACAAGGACGATAAATCAATCTATGATAAGTTGATTAAAGCTTATAACGTCAAATCGTTAACGACAGATGGTCAAAAAGTAAATAATAAAGACGGTAtt                                >  1:2765294/1‑100 (MQ=255)                                    gACAAGGACGATAAATCAATCTATGATAAGTTGATTAAAGCTTATAACGTCAAATCGTTAACGACAGATGGTCAAAAAGTAAATAATAAAGACGGTAttt                               <  1:6578320/100‑1 (MQ=255)                                                          aTGATAAGTTGATTAAAGCTTATAACGTCAAATCGTTAACGACAGATGGTCAAAAAGTAAATAATAAAGGCGGTATTTATCAAATTGTTAAAATTACGAc         >  1:2733052/1‑100 (MQ=255)                                                          aTGATAAGTTGATTAAAGCTTATAACGTCAAATCGTTAACGACAGATGGTCAAAAAGTAAATAATAAAGACGGTATTTATCAAATTGTTAAAATTACGAc         >  1:3167118/1‑100 (MQ=255)                                                          aTGATAAGTTGATTAAAGCTTATAACGTCAAATCGTTAACGACAGATGGTCAAAAAGTAAATAATAAAGACGGTATTTATCAAATTGTTAAAATTACGAc         >  1:3685009/1‑100 (MQ=255)                                                          aTGATAAGTTGATTAAAGCTTATAACGTCAAATCGTTAACGACAGATGGTCAAAAAGTAAATAATAAAGACGGTATTTATCAAATTGTTAAAATTACGAc         >  1:5543391/1‑100 (MQ=255)                                                          aTGATAAGTTGATTAAAGCTTATAACGTCAAATCGTTAACGACAGATGGTCAAAAAGTAAATAATAAAGACGGTATTTATCAAATTGTTAAAATTACGAc         >  1:5791844/1‑100 (MQ=255)                                                             aTAAGTTGATTAAAGCTTATAACGTCAAATCGTTAACGACAGATGGCCAAAAAGTAAATAATAAAGACGGTATTTATCAAATTGTTAAAATTACGACAGa      >  1:4216977/1‑100 (MQ=255)                                                                aGTTGATTAAAGCTTATAACGTCAAATCGTTAACGACAGATGGTCAAAAAGTAAATAATAAAGACGGTATTTATCAAATTGTTAAAATAACGACAGATcg   >  1:2535847/1‑99 (MQ=255)                                                                 cctGATTAAAGCTTATAACGTCAAATCGTTAACGACAGATGGTCAAAAAGTAAATAATAAAGACGGTATTTATCAAATTGTTAAAATTACGACAGATCaa  >  1:6358306/3‑100 (MQ=255)                                                                                         |                                                                            AAGATGAGGACATCGATCAGTTGCGTAAAGAGAAAGACAAGGACGATAAATCAATCTATGATAAGTTGATTAAAGCTTATAACGTCAATTCGTTAACGACAGATGGTCAAAAAGTAAATAATAAAGACGGTATTTATCAAATTGTTAAAATTACGACAGATCAA  >  NC_007793/240270‑240433

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑