Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,578,557	A→G	G23G (GGT→GGC)	SAUSA300_RS07710 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,578,557	0	A	G	100.0%	44.2 / NA	24	G23G (GGT→GGC)	SAUSA300_RS07710	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (1/23);  total (1/23)

TATTTATCTGTAATAGTTCCTTGATGTAATTCTTTGTGTTGGTAACTAGCCACTGTATAGATAGGCAATGTGATAACAAGTAGCAATGCGAATATACCGAATAATGACAGTATTCCAACAATAAAGATGTCGAACCCATCCATATTTTTAAGTTTTTTAATCATCATTGTCATCTCCAGTATCAATT  >  NC_007793/1578462‑1578648                                                                                                |                                                                                            tatTTATCTGTAATTGTTCCTTGGTGCGTTTCTTTGTGTTGGTAACTAGCCACTGTATAGATAGGAAATGTGACAACAAGTAACAATGCGAATATGCCgg                                                                                         <  1:3316740/100‑2 (MQ=14)               tgTTCCTTGGTGCGTTTCTTTGTGTTGGTAACTAGCCACTGTATAGATAGGAAATGTGACAACAAGTAACAATGCGAATATGCCGAATAATGACAGTact                                                                           <  1:5489666/99‑3 (MQ=14)                         tgcgtttCTTTGTGTTGGTAACTAGCCACTGTATAGATAGGAAATGTGACAACAAGTAACAATGCGAATATGCCGAATAATGACAGTACTCCAACAATaa                                                                 <  1:2252707/95‑1 (MQ=14)                         tgcgtttCTTTGTGTTGGTAACTAGCCACTGTATAGATAGGAAATGTGACAACAAGTAACAATGCGAATATGCCGAATAATGACAGTACTCCAACAATaa                                                                 <  1:5817031/95‑1 (MQ=14)                         tgcgtttCTTTGTGTTGGTAACTAGCCACTGTATAGATAGGAAATGTGACAACAAGTAACAATGCGAATATGCCGAATAATGACAGTACTCCAACAATaa                                                                 <  1:3221420/95‑1 (MQ=14)                         tgcgtttCTTTGTGTTGGTAACTAGCCACTGTATAGATAGGAAATGTGACAACAAGTAACAATGCGAATATGCCGAATAATGACAGTACTCCAACAATaa                                                                 <  1:4634423/95‑1 (MQ=14)                           cgtttCTTTGTGTTGGTAACTAGCCACTGTATAGATAGGAAATGTGACAACAAGTAACAATGCGAATATGCCGAATAATGACAGTACTCCAACAATAAAg                                                               <  1:5513359/97‑1 (MQ=14)                                                                        gacaaCAAGTAACAATGCGAATATGCCGAATAATGACAGTTCTCCAACAATAAAGATATCGAACCAATCCATATTTTTAAGTTTTTTAATCATCATTGTc                  <  1:827633/97‑1 (MQ=14)                                                                        gacaaCAAGTAACAATGCGAATATGCCGAATAATGACAGTACTCCAACAATAAAGATATCGAACCAATCCATATTTTTAAGTTTTTTAATCATCATTGTc                  <  1:2655141/97‑1 (MQ=14)                                                                        gacaaCAAGTAACAATGCGAATATGCCGAATAATGACAGTACTCCAACAATAAAGATATCGAACCAATCCATATTTTTAAGTTTTTTAATCATCATTGTc                  <  1:1074854/97‑1 (MQ=14)                                                                          caaCAAGTAACAATGCGAATATGCCGAATAATGACAGTACTCCAACAATAAAGATATCGAACCAATCCATATTTTTAAGTTTTTTAATCATCATTGTCaa                <  1:1597321/99‑2 (MQ=14)                                                                          caaCAAGTAACAATGCGAATATGCCGAATAATGACAGTACTCCAACAATAAAGATATCGAACCAATCCATATTTTTAAGTTTTTTAATCATCATTGTCAt                <  1:4967706/99‑1 (MQ=14)                                                                          caaCAAGTAACAATGCGAATATGCCGAATAATGACAGTACTCCAACAATAAAGATATCGAACCAATCCATATTTTTAAGTTTTTTAATCATCATTGTCAt                >  1:3260378/2‑100 (MQ=14)                                                                          caaCAAGTAACAATGCGAATATGCCGAATAATGACAGTACTCCAACAATAAAGATATCGAACCAATCCATATTTTTAAGTTTTTTAATCATCATTGTCAt                <  1:2765804/99‑1 (MQ=14)                                                                                gTAACAATGCGAACATGCCGAATAATGACAGTACTCCAACAATAAAGATATCGAACCAATCCATATTTTTAAGTTTTTTAATCATCATTGTCATCTCCAg          <  1:6025373/100‑1 (MQ=17)                                                                                  aacAATGCGAATATGCCGAATAATGACAGTTCTACAACAATAAAGATATCGAACCAATCCATATTTTTAAGTTTTTTAATCATCATTGTCATCTCCAGtc        <  1:5448165/98‑2 (MQ=17)                                                                                  aacAATGCGAATATGCCGAATAATGACAGTACTCCAACAATAAAGATATCGAACCAATCCATATTTTTAAGTTTTTTAATCATCATTGTCATCTCCAGTa        <  1:843059/98‑1 (MQ=18)                                                                                   acAATGCGAATATGCCGAATAATGACAGTACTCCAACAATAAAGATATCGAACCAATCCATATTTTTAAGTTTTTTAATCATCATTGTCATCTCCAGTaa       <  1:2325338/99‑2 (MQ=18)                                                                                    cAATGCGAATATGCCGAATAATGACAGTACTCCAACAATAAAGATATCGAACCAATCCATATTTTTAAGTTTTTTAATCATCATTGTCATCTCCAGTATc      <  1:3855946/100‑1 (MQ=18)                                                                                    cAATGCGAATATGCCGAATAATGACAGTACTCCAACAATAAAGATATCGAACCAATCCATATTTTTAAGTTTTTTAATCATCATTGTCATCTCCAGTATc      <  1:5869015/100‑1 (MQ=18)                                                                                     aaTGCGAATATGCCGAATAATTACAGTACTCCAACAATAAAGATATCGAACCAATCCATATTTTTAAGTTTTTTAATCACCATTGTCATCTCCAGTATCa     <  1:6053243/100‑1 (MQ=18)                                                                                     aaTGCGAATATGCCGAATAATGACAGTACTCCAACA‑‑AAAGATATCGAACCAATCCATATTTTTAAGTTTTTTAATCATCATTGTCATCTCCAGTATCaag   <  1:3162413/100‑2 (MQ=18)                                                                                      aTGCGAATATGCCGAATAATGACAGTACTCCAACAATAAAGATATCGAACCAATCCATATTTTTAAGTTTTTTAATCATCATTGTCATCTCCAGTATCag    <  1:6596744/100‑2 (MQ=18)                                                                                        gCGAATATGCCGAATAATGACAGTACTCCAACAATAAAGATATCGAACCAATCCATATTTTTAAGTTTTTTAATCATCATTGTCATCTCCAGTATCaact  <  1:5265046/100‑3 (MQ=18)                                                                                                |                                                                                            TATTTATCTGTAATAGTTCCTTGATGTAATTCTTTGTGTTGGTAACTAGCCACTGTATAGATAGGCAATGTGATAACAAGTAGCAATGCGAATATACCGAATAATGACAGTATTCCAACAATAAAGATGTCGAACCCATCCATATTTTTAAGTTTTTTAATCATCATTGTCATCTCCAGTATCAATT  >  NC_007793/1578462‑1578648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑