Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,578,362	G→A	S88S (AGC→AGT)	SAUSA300_RS07710 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,578,362	0	G	A	100.0%	28.4 / NA	13	S88S (AGC→AGT)	SAUSA300_RS07710	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (2/11);  total (2/11)

TAAAAAGTGTATTCTATAACCGATTGTTTTAACTTCTACCTTATCGCCTACTTTTAACCTAGCTTGTATATCTGCGCTATCAAATTTCTTTTTGAATAATAAG‑TCGGAATTTTCAATGACTTGTTTGTTGTCTAATACAATATAGAACTTGTCTTCTTTATCTTG  >  NC_007793/1578287‑1578451                                                                            |                                                                                           tAAAAAGTGTATTCTATATCCAATTGTCTTAACTTCTACTTTATCGCCTACTTTTAACCTAGCTTGTATATCTGCACTATCAAATTTCTTTTTGaataat                                                                    <  1:4127046/100‑1 (MQ=17)                atatCCAATTGTCTTAACTTCTACTTTATCGCCTACTTTTAACCTAGCTTGTATATCTGCACTATCAAATTTCTTTTTGAATAATAAGATCGGaagagca                                                     >  1:2938619/1‑95 (MQ=17)                       cttGTCTTAACTTCTACTTTATCGCCTACTTTTAACCTAGCTTGTATATCTGCACTATCAAATTTCTTTTTGAATAATAAG‑TCGGAATTTTCAATGAcag                                             <  1:2262112/99‑3 (MQ=18)                             cgatcTTCTACTTTATCGCCTACTTTTAACCTAGCTTGTATATCTGCACTATCAAATTTCTTTTTGAATAATAAG‑TCGGAATTTTCAATGActtgcttgg                                       <  1:3456511/96‑2 (MQ=21)                                  ttCTACTTTATCGCCTACTTTTAACCTAGCTTGTATATCTGCACTATCAAATTTCTTTTTGAATAATAAG‑TCGGAATTTTCAATGACTTGCTTGTTGTCt                                  <  1:3883195/100‑1 (MQ=255)                                       ctttATCGCCTGCTTTTAACCTAGCTTGTATATCTGCACTATCAAATTTCATTTTGAATAATAAG‑TCGGAATTTTCAATGACTTGCTTGTTGTCTAATAc                             <  1:3441368/98‑1 (MQ=21)                                            tCGCCTGCTTTTAACCTAGCTTGTATATCTGCACTATCAAATTTCTTTTTGAATAATAAG‑TCGGAATTTTCAATGACTTGCTTGTTGTCTAATACAatat                        <  1:3651335/100‑1 (MQ=255)                                            tCGCCTACTTTTAACCTAGCTTGTATATCTGCACTATCAAATTTCTTTTTGAATAATAAG‑TCGGAATTTTCAATGACTTGCTTGTTGTCTAATACAatat                        <  1:891206/100‑1 (MQ=255)                                                    ttttAACCTAGCTTGTATATCTGCACTATCAAATTTCTTTTTGAATAATAAG‑TCGGAATTTTCAATGACTTGCTTGTTGTCTAATACAATATAGAACTTg                <  1:4090214/100‑1 (MQ=255)                                                    ttttAACCTAGCTTGTATATCTGCACTATCAAATTTCTTTTTGAATAATAAG‑TCGGAATTTTCAATGACTTGCTTGTTGTCTAATACAATATAGAACTTg                <  1:5097385/100‑1 (MQ=255)                                                     tttAACCTAGCTTGTATATCTGCACTATCAAATTTCTTTTTGAATAATAAG‑TCGGAATTTTCAATGACTTGCTTGTTGTCTAATACAATATAGAACTTgg               <  1:5760792/100‑2 (MQ=255)                                                          ccTAGCTTGTATATCTGCACTATCAAATTTCTTTTTGAATAATAAG‑TCGGAATTTTCAATGACTTGCTTGTTGTCTAATACAATACAGAACTTGtcttct          >  1:2450825/1‑100 (MQ=255)                                                                  gTATATCTGCACTATCAAATTTCTTTTTGAATAATAAG‑TCGGAATTTTCAATGACTTGCTTGTTGTCTAATACAATATAGAACTTGTCTTCTTTATCTTg  <  1:412345/100‑1 (MQ=255)                                                                            |                                                                                           TAAAAAGTGTATTCTATAACCGATTGTTTTAACTTCTACCTTATCGCCTACTTTTAACCTAGCTTGTATATCTGCGCTATCAAATTTCTTTTTGAATAATAAG‑TCGGAATTTTCAATGACTTGTTTGTTGTCTAATACAATATAGAACTTGTCTTCTTTATCTTG  >  NC_007793/1578287‑1578451

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑