Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,578,244	G→A	Q128* (CAA→TAA)	SAUSA300_RS07710 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,578,244	0	G	A	100.0%	19.7 / NA	12	Q128* (CAA→TAA)	SAUSA300_RS07710	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/7);  total (5/7)

TTAGCCGTCATCATAATATACACTTGCTCAGTTACATACTTACCTAACTCATACATTGCTAGTAAGAATAATAGTCTTAGTATTTGTTTAATCATTGTTTATCTACCTTCTTTACTTCGTATAAGACCGGATATAAATTTAAAAAGTGTATTCTA  >  NC_007793/1578148‑1578302                                                                                                 |                                                           cttgCCGTCATCATAATATATACTTGCTCCGTTACATACTTACCTAGCTCATACATTGCTAGCAAGAATAATAGTCTTAATATTTGTTTAATCATTAttt                                                         >  1:5527914/4‑100 (MQ=11)           tcatATTATATACTTGCTCCGTTACATACTTACCTAGCTCATACATTGCTAGCAAGAATAATAGTCTTAATATTTGTTTAATCATTATTTATCTACcttc                                               >  1:4805120/1‑100 (MQ=11)            catAATATATACTTGCTCCGTTACATACTTACCTAGCTCATACATTGCTAGCAAGAATAATAGTCTTAATATTTGTTTAATCATTATTTATCTACcttat                                              <  1:2892762/100‑3 (MQ=11)                        ttGCTCCGTTACATACTTACCTAGCTCATACATTGCTAGCAAGAATAATAGTCTTAATATTTGTTTAATCATTATTTATCTACCTTCTTTACTTGGtata                                  <  1:3814432/100‑1 (MQ=14)                        ttGCTCCGTTACATACTTACCTAGCTCATACATTGCTAGCAAGAATAATAGTCTTAATATTTGTTTAATCATTATTTATCTACCTTCTTTACTTCGtata                                  <  1:4572208/100‑1 (MQ=14)                        ttGCTCCGTTACATACTTACCTAGCTCATACATTGCTAGCAAGAATAATAGTCTTAATATTTGTTTAATCATTATTTATCTACCTTCTTTACTTCGtata                                  <  1:5755090/100‑1 (MQ=14)                         tGCTCTGTTACATACTTACCTAGCTCATACATTGCTAGCAAGAATAATAGTCTTAATATTTGTTTAATCATTATTTATCTACCTTCTTTACTTCGTACaa                                 >  1:3984072/1‑100 (MQ=14)                         tGCTCCGTTACATACTTACCTAGCTCATACATTGCTAGCAAGAATAATAGTCTTAATATTTGTTTAATCATTATTTATCTACCTTCTTTACTTCGTATaa                                 >  1:1897309/1‑100 (MQ=14)                                                    tACATTGCTAGCAAGAATAATAGTCTTAATATTTGTTTAATCATTATTTATCTACCTTCTTTACTTCGTATAAGACCGGATATAAATTTAAAAAGTGTAt      >  1:5921590/1‑100 (MQ=255)                                                     aCATTGCTAGCAAGAATAATAGTCTTAATATTTGTTTAATCATTATTTATCTACCTTCTTTTCTTCGTATAAGACCGGATATAAATTTAAAAAGTGTAta     <  1:5623273/100‑2 (MQ=14)                                                     aCATTGCTAGCAAGAATAATAGTCTTAATATTTGTTTAATCATTATTTATCTACCTTCTTTACTTCGTATAAGACCGGATATAAATTTAAAAAGTGTAtc     <  1:5917526/100‑2 (MQ=255)                                                        ctGCTAGCAAGAATAATAGTCTTAATATTTGTTTAATCATTATTTATCTACCTTCTTTACTTCGTATAAGACCGGATATAAATTTAAAAAGTGTATTCta  <  1:3174198/99‑1 (MQ=255)                                                                                                 |                                                           TTAGCCGTCATCATAATATACACTTGCTCAGTTACATACTTACCTAACTCATACATTGCTAGTAAGAATAATAGTCTTAGTATTTGTTTAATCATTGTTTATCTACCTTCTTTACTTCGTATAAGACCGGATATAAATTTAAAAAGTGTATTCTA  >  NC_007793/1578148‑1578302

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑