Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,578,227	G→A	L6L (CTA→TTA)	SAUSA300_RS07705 ←	transcriptional activator RinB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,578,227	0	G	A	100.0%	21.3 / NA	13	L6L (CTA→TTA)	SAUSA300_RS07705	transcriptional activator RinB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/7);  total (6/7)

CTACATCATCATTAGCCGTCATCATAATATACACTTGCTCAGTTACATACTTACCTAACTCATACATTGCTAGTAAGAATAATAGTCTTAGTATTTGTTTAATCATTGTTTATCTACCTTCTTTACTTCGTATAAGACCGGATATAAATTTAAAAAGTGTATTCTA  >  NC_007793/1578137‑1578302                                                                                           |                                                                            ctACATCATCATTAGCCGTCATCATAATATATACTTGCTCCGTTACATACTTACCTAGCTCATACATTGCTAGCAAGAATAATAGTCTTAATATTTGttt                                                                    >  1:353338/1‑100 (MQ=11)            cttgCCGTCATCATAATATATACTTGCTCCGTTACATACTTACCTAGCTCATACATTGCTAGCAAGAATAATAGTCTTAATATTTGTTTAATCATTAttt                                                         >  1:5527914/4‑100 (MQ=11)                      tcatATTATATACTTGCTCCGTTACATACTTACCTAGCTCATACATTGCTAGCAAGAATAATAGTCTTAATATTTGTTTAATCATTATTTATCTACcttc                                               >  1:4805120/1‑100 (MQ=11)                       catAATATATACTTGCTCCGTTACATACTTACCTAGCTCATACATTGCTAGCAAGAATAATAGTCTTAATATTTGTTTAATCATTATTTATCTACcttat                                              <  1:2892762/100‑3 (MQ=11)                                   ttGCTCCGTTACATACTTACCTAGCTCATACATTGCTAGCAAGAATAATAGTCTTAATATTTGTTTAATCATTATTTATCTACCTTCTTTACTTGGtata                                  <  1:3814432/100‑1 (MQ=14)                                   ttGCTCCGTTACATACTTACCTAGCTCATACATTGCTAGCAAGAATAATAGTCTTAATATTTGTTTAATCATTATTTATCTACCTTCTTTACTTCGtata                                  <  1:4572208/100‑1 (MQ=14)                                   ttGCTCCGTTACATACTTACCTAGCTCATACATTGCTAGCAAGAATAATAGTCTTAATATTTGTTTAATCATTATTTATCTACCTTCTTTACTTCGtata                                  <  1:5755090/100‑1 (MQ=14)                                    tGCTCTGTTACATACTTACCTAGCTCATACATTGCTAGCAAGAATAATAGTCTTAATATTTGTTTAATCATTATTTATCTACCTTCTTTACTTCGTACaa                                 >  1:3984072/1‑100 (MQ=14)                                    tGCTCCGTTACATACTTACCTAGCTCATACATTGCTAGCAAGAATAATAGTCTTAATATTTGTTTAATCATTATTTATCTACCTTCTTTACTTCGTATaa                                 >  1:1897309/1‑100 (MQ=14)                                                               tACATTGCTAGCAAGAATAATAGTCTTAATATTTGTTTAATCATTATTTATCTACCTTCTTTACTTCGTATAAGACCGGATATAAATTTAAAAAGTGTAt      >  1:5921590/1‑100 (MQ=255)                                                                aCATTGCTAGCAAGAATAATAGTCTTAATATTTGTTTAATCATTATTTATCTACCTTCTTTTCTTCGTATAAGACCGGATATAAATTTAAAAAGTGTAta     <  1:5623273/100‑2 (MQ=14)                                                                aCATTGCTAGCAAGAATAATAGTCTTAATATTTGTTTAATCATTATTTATCTACCTTCTTTACTTCGTATAAGACCGGATATAAATTTAAAAAGTGTAtc     <  1:5917526/100‑2 (MQ=255)                                                                   ctGCTAGCAAGAATAATAGTCTTAATATTTGTTTAATCATTATTTATCTACCTTCTTTACTTCGTATAAGACCGGATATAAATTTAAAAAGTGTATTCta  <  1:3174198/99‑1 (MQ=255)                                                                                           |                                                                            CTACATCATCATTAGCCGTCATCATAATATACACTTGCTCAGTTACATACTTACCTAACTCATACATTGCTAGTAAGAATAATAGTCTTAGTATTTGTTTAATCATTGTTTATCTACCTTCTTTACTTCGTATAAGACCGGATATAAATTTAAAAAGTGTATTCTA  >  NC_007793/1578137‑1578302

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑