Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	234,362	G→A	S223N (AGC→AAC)	SAUSA300_RS01050 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	234,362	0	G	A	100.0%	103.7 / NA	39	S223N (AGC→AAC)	SAUSA300_RS01050	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (38/1);  total (38/1)

TCTAGAATAGTTAAGTTCATTACAGAGAAAAGTGAACTAATGTCACCACCAAAGGGATTTGCAACGGCAGGAAGATTGAATTCTAAATGGATAAGCGTATTATACCTTAGTACAAAAGAACAGGTCAGTATAGAAGAAGTTAAACCTAAGCATAATGACATTATTTATATAGGTAAGGTTAAGT  >  NC_007793/234268‑234451                                                                                               |                                                                                          gcTAGAATAGTTAAGTTCATTACAGAGAAAAGTGAACTAATGTCACCACCAAAGGGATTTGCAACGGCAGGAAGATTGAATTCTAAATGGATAAACGtat                                                                                      >  1:2739988/2‑100 (MQ=255)    aGAATAGTTAAGTTCATTACAGAGAAAAGTGAACTAATGTCGCCACCAAAGGGATTTGCAACGGCAGGAAGATTGAATTCTAAATGGATAAACGtattat                                                                                   >  1:116514/1‑100 (MQ=255)    aGAATAGTTAAGTTCATTACAGAGAAAAGTGAACTAATGTCACCACCAAAGGGATTTGCAACGGCAGGAAGATTGAATTTTAAATGGATAAACGtattat                                                                                   >  1:5009828/1‑100 (MQ=255)    aGAATAGTTAAGTTCATTACAGAGAAAAGTGAACTAATGTCACCACCAAAGGGATTTGCAACGGCAGGAAGATTGAATTCTAAATGGATAAACGtattat                                                                                   >  1:3694609/1‑100 (MQ=255)    aGAATAGTTAAGTTCATTACAGAGAAAAGTGAACTAATGTCACCACCAAAGGGATTTGCAACGGCAGGAAGATTGAATTCTAAATGGATAAACGtattat                                                                                   >  1:4469367/1‑100 (MQ=255)    aGAATAGTTAAGTTCATTACAGAGAAAAGTGAACTAATGTCACCACCAAAGGGATTTGCAACGGCAGGAAGATTGAATTCTAAATGGATAAACGtattat                                                                                   >  1:1035107/1‑100 (MQ=255)    aGAATAGTTAAGTTCATTACAGAGAAAAGTGAACTAATGTCACCACCAAAGGGATTTGCAACGGCAGGAAGATTGAATTCTAAATGGATAAACGtattat                                                                                   >  1:6605689/1‑100 (MQ=255)    aGAATAGTTAAGTTCATTACAGAGAAAAGTGAACTAATGTCACCACCAAAGGGATTTGCAACGGCAGGAAGATTGAATTCTAAATGGATAAACGtattat                                                                                   >  1:6600622/1‑100 (MQ=255)    aGAATAGTTAAGTTCATTACAGAGAAAAGTGAACTAATGTCACCACCAAAGGGATTTGCAACGGCAGGAAGATTGAATTCTAAATGGATAAACGtattat                                                                                   >  1:6149558/1‑100 (MQ=255)    aGAATAGTTAAGTTCATTACAGAGAAAAGTGAACTAATGTCACCACCAAAGGGATTTGCAACGGCAGGAAGATTGAATTCTAAATGGATAAACGtattat                                                                                   >  1:6129717/1‑100 (MQ=255)    aGAATAGTTAAGTTCATTACAGAGAAAAGTGAACTAATGTCACCACCAAAGGGATTTGCAACGGCAGGAAGATTGAATTCTAAATGGATAAACGtattat                                                                                   >  1:5640140/1‑100 (MQ=255)    aGAATAGTTAAGTTCATTACAGAGAAAAGTGAACTAATGTCACCACCAAAGGGATTTGCAACGGCAGGAAGATTGAATTCTAAATGGATAAACGtattat                                                                                   >  1:5934578/1‑100 (MQ=255)    aGAATAGTTAAGTTCATTACAGAGAAAAGTGAACTAATGTCACCACCAAAGGGATTTGCAACGGCAGGAAGATTGAATTCTAAATGGATAAACGtattat                                                                                   >  1:573917/1‑100 (MQ=255)    aGAATAGTTAAGTTCATTACAGAGAAAAGTGAACTAATGTCACCACCAAAGGGATTTGCAACGGCAGGAAGATTGAATTCTAAATGGATAAACGtattac                                                                                   >  1:3118243/1‑99 (MQ=255)    aGAATAGTTAAGTTCATTACAGAGAAAAGTGAACTAATGTCACCACCAAAGGGATTTGCAACGGCAGGAAGATTGAATTCCAAATGGATAAACGtattat                                                                                   >  1:688784/1‑100 (MQ=255)                  cATTACAGAGAAAAGTGAACTAATGTCACCACCAAAGGGATTTGCAACGGCAGGAAGATTGAATTCTAAATGGATAAACGTATTATACCTTAGTACaaaa                                                                     >  1:6037256/1‑100 (MQ=255)                   aTTACAGAGAAAAGTGAACTAATGTCACCACCAAAGGGATTTGCAACGGCAGGAAGATTGAATTCTAAATGGATAAACGTATTATACCTTAGTACAAAAg                                                                    >  1:3136018/1‑100 (MQ=255)                     tACAGAGAAAAATGTACTAATGTCACCGCCAAAGGGCTTTGCTACGGCAGGAAGATTGAAGTCTAAATGGTTAAACGTATTATACCTTAGTACAATAGaa                                                                  >  1:6289827/1‑100 (MQ=255)                      aCAGAGAAAAGTGAACTAATGTCACCACCAAAGGGATTTGCAACGGCAGGAAGATTGAATTCTAAATGGATAAACGTATTATACCTTAGTACAAAAGAAc                                                                 <  1:113224/100‑1 (MQ=255)                        agagAAAAGTGAACTAATGTCACCACCAAAGGGATTTGCAACGGCAGGAAGATTGAATTCTAAATGGATAAACGTATTATACCTTAGTACAAAAGAACAg                                                               >  1:1175685/1‑100 (MQ=255)                                       aaTGTCACCACCAAAGGGATTTGCAACGGCAGGAAGATTGAATTCTAAATGGATAAACGTATTATATCTTAGTACAAAAGAACAGGCCAgatcggaagag                                                >  1:1741137/1‑90 (MQ=255)                                         gcccaccacCAAAGGGATTTGCAACGGCAGGAAGATTGAATTCTAAATGGATAAACGTATTATACCTTAGTACAAAAGAACAGGTCAGTATAGAAGAAGt                                              >  1:3477712/4‑100 (MQ=255)                                                 ccaAAGGGATTTGCAACGGCAGGAAGATTGAATTCTAAATGGATAAACGTATTATACCTTAGTACAAAAGAACAGGTCAGTATAGAAGAAGTTAAACCTa                                      >  1:2651386/1‑100 (MQ=255)                                                        aaTTTGCAACGGCAGGAAGATTGAATTCTAAATGGATAAACGTATTATACCTTAGTACAAAAGAACAGGTCAGTATAGAAGAAGTTAAACCTAAGCATaa                               >  1:268967/2‑100 (MQ=255)                                                           ttGCAACGGCAGGAAGATTGAATTCTAAATGGATAAACGTATTATACCTTAGTACAAAAGAACAGGTCAGTATAGAAGAAGTTAAACCTAAGCATAATGa                            >  1:2285022/1‑100 (MQ=255)                                                           ttGCAACGGCAGGAAGATTGAATTCTAAATGGATAAACGTATTATACCTTAGTACAAAAGAACAGGTCAGTATAGAAGAAGTTAAACCTAAGCATAATGa                            >  1:234628/1‑100 (MQ=255)                                                            tGCAACGGCAGGAAGATTGAATTCTAAATGGATAAACGTATTATACCTTAGTACAAAAGAACAGGTCAGTATAGAAGAAGTTAAACCTAAGCagatcgga                           >  1:5362138/1‑93 (MQ=255)                                                             gCAACGGCAGGAAGATTGAATTCTAAATGGATAAACGTATTATACCTTAGTACAAAAGAACAGGTCAGTATAGAAGAAGTTAAACCTAAGCATAATGACa                          >  1:299887/1‑100 (MQ=255)                                                             gCAACGGCAGGAAGATTGAATTCTAAATGGATAAACGTATTATACCTTAGTACAAAAGAACAGGTCAGTATAGAAGAAGTTAAACCTAAGCATAATGACa                          >  1:5967155/1‑100 (MQ=255)                                                              ataCGGCAGGAAGATTGAATTCTAAATGGATAAACGTATTATACCTTAGTACAAAAGAACAGGTCAGTATAGAAGAAGTTAAACCTAAGCATAATGACat                         >  1:3236610/3‑100 (MQ=255)                                                                gcGGCAGGAAGATTGAATTCTAAATGGATAAACGTATTATACCTTAGTACAAAAGCACAGGTCAGTATAGAAGAAGTTAAACCTAAGCATAATGACatta                       >  1:2590563/2‑100 (MQ=255)                                                                aCGGCAGGAAGATTGAATTCTAAATGGATAAACGTATTATACCTTAGTACAAAAGAACAGGTCAGTATAGAAGAAGTTAAACCTAAGCATAATGACatta                       >  1:1082300/1‑100 (MQ=255)                                                                aCGGCAGGAAGATTGAATTCTAAATGGATAAACGTATTATACCTTAGTACAAAAGAACAGGTCAGTATAGAAGAAGTTAAACCTAAGCATAATGACatta                       >  1:2149112/1‑100 (MQ=255)                                                                aCGGCAGGAAGATTGAATTCTAAATGGATAAACGTATTATACCTTAGTACAAAAGAACAGGTCAGTATAGAAGAAGTTAAACCTAAGCATAATGACatta                       >  1:2779351/1‑100 (MQ=255)                                                                   tcAGGAAGATTGAATTCTAAATGGATAAACGTATTATACCTTAGTACAAAAGAACAGGTCAGTATAGAAGAAGTTAAAACTAAGCATAATGACAttattt                    >  1:4103117/2‑100 (MQ=255)                                                                    cAGGAAGATTGAATTCTAAATGGATAGACGTATTATACCTTAGTACAAAAGAACAGGTCAGTATAGAAGAAGTTAAACCTAAGCATAATGACAttattta                   >  1:5732377/1‑100 (MQ=255)                                                                             tGAATTCTAAATGGATAAACGTATTATACCTTAGTACAAAAGAACAGGTCAGTATAGAAGAAGTTAAACCTAAGCATAATGACATTATTTATAAAGGTaa          >  1:6349279/1‑100 (MQ=255)                                                                                aTTCTAAATGGATAAACGTATTATACCTTAGTACAAAAGAACAGGTCAGTATAGAAGAAGTTAAACCTAAGCATAATGACATTATTTATATAGGTAAGGt       >  1:354101/1‑100 (MQ=255)                                                                                     aaaTGGATAAACGTATTATACCTTAGTACAAAAGAACAGGTCAGTATAGAAGAAGTTAAACCTAAGCATAATGACATTATTTATATAGGTAAGGTTAAGt  >  1:1614433/1‑100 (MQ=255)                                                                                               |                                                                                          TCTAGAATAGTTAAGTTCATTACAGAGAAAAGTGAACTAATGTCACCACCAAAGGGATTTGCAACGGCAGGAAGATTGAATTCTAAATGGATAAGCGTATTATACCTTAGTACAAAAGAACAGGTCAGTATAGAAGAAGTTAAACCTAAGCATAATGACATTATTTATATAGGTAAGGTTAAGT  >  NC_007793/234268‑234451

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑