Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,578,194	A→G	L17L (TTA→CTA)	SAUSA300_RS07705 ←	transcriptional activator RinB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,578,194	0	A	G	100.0%	10.9 / NA	10	L17L (TTA→CTA)	SAUSA300_RS07705	transcriptional activator RinB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (5/5);  total (5/5)

TCTGATTTTTTCGTAATCACTCGGCGCCTCTACATCATCATTAGCCGTCATCATAATATACACTTGCTCAGTTACATACTTACCTAACTCATACATTGCTAGTAAGAATAATAGTCTTAGTATTTGTTTAATCATTGTTTATCTACCTTCTTTACTTCGTATAA  >  NC_007793/1578108‑1578271                                                                                       |                                                                              cgctcgccaatCGTAATCACCCGTTGCCCCTACATCATCATTAGCCTTCCCCATAATTTATACTTGCTCCGTTCCATTCTTACCGAGCTCATTCATTGCt                                                                  <  1:6266295/90‑1 (MQ=2)                              ctACATCATCATTAGCCGTCATCATAATATATACTTGCTCCGTTACATACTTACCTAGCTCATACATTGCTAGCAAGAATAATAGTCTTAATATTTGttt                                     >  1:353338/1‑100 (MQ=11)                                         cttgCCGTCATCATAATATATACTTGCTCCGTTACATACTTACCTAGCTCATACATTGCTAGCAAGAATAATAGTCTTAATATTTGTTTAATCATTAttt                          >  1:5527914/4‑100 (MQ=11)                                                   tcatATTATATACTTGCTCCGTTACATACTTACCTAGCTCATACATTGCTAGCAAGAATAATAGTCTTAATATTTGTTTAATCATTATTTATCTACcttc                >  1:4805120/1‑100 (MQ=11)                                                    catAATATATACTTGCTCCGTTACATACTTACCTAGCTCATACATTGCTAGCAAGAATAATAGTCTTAATATTTGTTTAATCATTATTTATCTACcttat               <  1:2892762/100‑3 (MQ=11)                                                                ttGCTCCGTTACATACTTACCTAGCTCATACATTGCTAGCAAGAATAATAGTCTTAATATTTGTTTAATCATTATTTATCTACCTTCTTTACTTGGtata   <  1:3814432/100‑1 (MQ=14)                                                                ttGCTCCGTTACATACTTACCTAGCTCATACATTGCTAGCAAGAATAATAGTCTTAATATTTGTTTAATCATTATTTATCTACCTTCTTTACTTCGtata   <  1:4572208/100‑1 (MQ=14)                                                                ttGCTCCGTTACATACTTACCTAGCTCATACATTGCTAGCAAGAATAATAGTCTTAATATTTGTTTAATCATTATTTATCTACCTTCTTTACTTCGtata   <  1:5755090/100‑1 (MQ=14)                                                                 tGCTCTGTTACATACTTACCTAGCTCATACATTGCTAGCAAGAATAATAGTCTTAATATTTGTTTAATCATTATTTATCTACCTTCTTTACTTCGTACaa  >  1:3984072/1‑100 (MQ=14)                                                                 tGCTCCGTTACATACTTACCTAGCTCATACATTGCTAGCAAGAATAATAGTCTTAATATTTGTTTAATCATTATTTATCTACCTTCTTTACTTCGTATaa  >  1:1897309/1‑100 (MQ=14)                                                                                       |                                                                              TCTGATTTTTTCGTAATCACTCGGCGCCTCTACATCATCATTAGCCGTCATCATAATATACACTTGCTCAGTTACATACTTACCTAACTCATACATTGCTAGTAAGAATAATAGTCTTAGTATTTGTTTAATCATTGTTTATCTACCTTCTTTACTTCGTATAA  >  NC_007793/1578108‑1578271

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑