Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,534,963	A→T	G123G (GGA→GGT)	SAUSA300_RS07470 →	L‑asparaginase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,534,963	0	A	T	100.0%	32.7 / NA	14	G123G (GGA→GGT)	SAUSA300_RS07470	L‑asparaginase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (14/0);  total (14/0)

AACTGCCTTTTTACTTGATTTAATATTAGGTATCGAGCAACCTGTTGTTATTACTGGCGCAATGCGCTCGTCTAATGAAATTGGTTCTGACGGATTATATAATTATA‑TTTCCGCTATTCGCGTTGCCTCTGATGAAAAGGCCCGTCATAAAGGCGTGATGGTTGTATTTAATGATGAAATTCATACGGC  >  NC_007793/1534870‑1535058                                                                                              |                                                                                                 aaCTGCCTTTTTACTTGATTTAATATTAGGTATCGAGCAACCTGTTGTTATTACTGGCGCAATGCGCTCGTCTAATGAAATTGGTTCTGACGGTTtatat                                                                                            >  1:6153854/1‑100 (MQ=255)  aCTGCCTTTTTACTTGATTTAATATTAGGTATCGAGCAACCTGTTGTTATTACTGGCGCAATGCGCTCGTCTAATGAAATTGGTTCTGACGGTTtatata                                                                                           >  1:1755668/1‑100 (MQ=255)                            aGGTGTCGAGCAACCTGTTGTTATTACTGGCGCAATGCGCTCGTCTAATGAAATTGGTTCTGACGGTTTATATAATTATA‑TTTCCGCTATTCGCGTTGcc                                                                >  1:5960172/1‑100 (MQ=255)                                       taCCTGTTGTTATTACTGGCGCAATGCGCTCGTCTAATGAAATTGGTTCTGACGGTTTATATAATTATA‑TTTCCGCTATTCGCGTTGCCTCTGATGaaaa                                                     >  1:90337/2‑100 (MQ=255)                                       gaCCTGTTGTTATTACTGGCGCAATGCGCTCGTCTAATGAAATTGGTTCTGACGGTTTATATAATTATA‑TTTCCGCTATTCGCGTTGCCTCTGATGaaaa                                                     >  1:1995153/2‑100 (MQ=255)                                       aaCCTGTTGTTATTACTGGCGCAATGCGCTCGTCTAATGAAATTGGTTCTGACGGTTTATATAATTATA‑TTTCCGCTATTCGCGTTGCCTCTGATGaaaa                                                     >  1:949903/1‑100 (MQ=255)                                        aCCTGTTGTTATTACTGGCGCAATGCGCTCGTCTAATGAAATTGGTTCTGACGGTTTATATAATTATA‑TTTCCGCTATTCGCGCTGCCTCTGATGAAAAg                                                    >  1:13707/1‑100 (MQ=255)                                        aCCCGTTGTTATTACTGGCGCAATGCGCTCGTCTAATGAAATTGGTTCTGACGGTTTATATAATTATATTTTCCGCTATTCGCGTTGCCTCTGATGaaaa                                                     >  1:1778838/1‑100 (MQ=255)                                          cTGTTGTTATTACTGGCGCAATGCGCTCGTCTAATGAAATTGGTTCTGACGGTTTATATAATTATA‑TTTCCGCTATTCGCGTTGCCTCTGATGAAAAGGc                                                  >  1:3271914/1‑100 (MQ=255)                                                                          aaTGAAATTGGTTCTGACGGTTTATATAATTATA‑TTTCCGCTATTCGCGTCGCCTCTGATGAAAAGGCCCGTCCTAAAGGCGTGATGGTTGTATTTAatg                  >  1:4498482/1‑100 (MQ=255)                                                                                     gccTGACGGTTTATATAATTATA‑TTTCCGCTATTCGCGTTGCCTCTGATGAAAAGGCCCGTCATAAAGGCGTGATGGTTGTATTTAATGATGAAATTCAt       >  1:635755/3‑100 (MQ=255)                                                                                         gACGGTTTATATAATTATA‑TTTCCGCTATTCGCGTTGCCTCTGATGAAAAGGCCCGTCATAAAGGCGTGATGGTTGTATTTAATGATGAAATTCATACgg   >  1:1539841/1‑100 (MQ=255)                                                                                         gACGGTTTATATAATTATA‑TTTCCGCTATTCGCGTTGCCTCTGATGAAAAGGCCCGTCATAAAGGCGTGATGGTTGTATTTAATGATGAAATTCATACgg   >  1:458593/1‑100 (MQ=255)                                                                                          aCGGTTTATATAATTATA‑TTTCCGCTATTCGCGTTGCCTCTGATGAACAGTCCCGTCATAAAGGCGTGATGGTTGTATTTAATGATGAAATTCATACGgc  >  1:186581/1‑100 (MQ=255)                                                                                              |                                                                                                 AACTGCCTTTTTACTTGATTTAATATTAGGTATCGAGCAACCTGTTGTTATTACTGGCGCAATGCGCTCGTCTAATGAAATTGGTTCTGACGGATTATATAATTATA‑TTTCCGCTATTCGCGTTGCCTCTGATGAAAAGGCCCGTCATAAAGGCGTGATGGTTGTATTTAATGATGAAATTCATACGGC  >  NC_007793/1534870‑1535058

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑