| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_007793 | 1,499,109 | A→T | N122I (AAT→ATT) | SAUSA300_RS07270 → | sulfite reductase subunit alpha |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_007793 | 1,499,109 | 0 | A | T | 93.3% | 31.2 / ‑4.6 | 15 | N122I (AAT→ATT) | SAUSA300_RS07270 | sulfite reductase subunit alpha |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base T (12/2); minor base C (1/0); total (13/2) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.10e-01 | |||||||||||
ACACTGAGTGATATGGCAGGAAATCAACGTTACGTCTTTCTAGATCATAACAATCAATTTGTTGCAGATCAAAATGCGAAACATACGTATTCTAATGTGATGCGAAGTATACATAGTTCTTTGTTTACTGAAAATACTATTATTAATTATTTAGTAGAGTTAACCGCATGTTGGATGATA > NC_007793/1499015‑1499194 | acacTGAGTGATATGGCAGGAAATCAACGTTACGTCTTTCTAGATCATAACAATCAATTTGTTGCAGATCAAAATGCGAAACATACGTATTCTATTGTGa > 1:6411639/1‑100 (MQ=255) tacTGAGTGATATGGCAGGAAATCAACGTTACGTCTTTCTAGATCATAACAATCAATTTGTTGCAGATCAAAATGCGAAACATACGTATTCTATTGTGAt > 1:398226/2‑100 (MQ=255) aGTGATATGGCAGGAAATCAACGTTACGTCTTTCTAGATCATAACAATCAATTTGTTGCAGATCAAAATGCGAAACATACGTATTCTATTGTGATGCGaa > 1:504496/1‑100 (MQ=255) gtatGGCAGGAAATCAACGTTACGTCTTTCTAGATCATAACAATCAATTTGTTGCAGATCAAAATGCGAAACATACGTATTCTATTGTGATGCGAAGtat > 1:1456323/2‑100 (MQ=255) atGGCAGGAAATCAACGTTACGTCTTTCTAGATCATAACAATCAATTTGTTGCAGATCAAAATGCGAAACATACGTATTCTATTGTGATGCGAAGTATAc > 1:3373141/1‑100 (MQ=255) atGGCAGGAAATCAACGTTACGTCTTTCTAGATCATAACAATCAATTTGTTGCAGATCAAAATGCGAAACATACGTATTCTATTGTGATGCGAAGTATAc > 1:5263916/1‑100 (MQ=255) atGGCAGGAAATCAACGTTACGTCTTTCTAGATCATAACAATCAATTTGTTGCAGATCAAAATGCGAAACATACGTATTCTACTGGGATCGGAAgagcac > 1:4946957/1‑95 (MQ=255) ggCAGGAAATCAACGTTACGTCTTTCTAGATCATAACAATCAATTTGTTGCAGATCAAAATGCGAAACATACGTATTCTATTGTGATGCGAAGTATACAt > 1:923441/1‑100 (MQ=255) caTCAACGTTACGTCCTTCTAGATCATAACAATCAATTTGTTGCAGATCAAAATGCGAAACATACGTATTCTATTGTGATGCGAAGTATACATAGTTCtt > 1:4253853/2‑100 (MQ=255) aaTCAACGTTACGTCTTTCTAGATCATAACAATCAATTTGTTGCAGATCAAAATGCGAAACATACGTATTCTATTGTGATGCGAAGTATACATAGTTCtt > 1:5051268/1‑100 (MQ=255) aacaCGTTACGTCTTTCTAGATCATAACAATCAATTTGTTGCAGATCAAAATGCGAAACATACGTATTCTATTGTGATGCGAAGTATACATAGTTCTTTg > 1:3462149/4‑100 (MQ=255) aCGTCTTTCTAGATCATAACAATCAATTTGTTGCAGATCAAAATGCGAAACATACGTATTCTATTGTGATGCGAAGTATACATAGTTCTTTGTTTACTGa > 1:21938/1‑100 (MQ=255) aaTGCGAAACATACGTATTCTATTGTGATGCGAAGTATACATAGTTCTTTGTTTACTGAAAATACTATTATTAGTTATTTAGTAGAGTTAACCGCATGtg < 1:3771793/100‑2 (MQ=255) cGAAACATACGTATTCTATTGTGATGCGAAGTATACATAGTTCTTTGTTTACTGAAAATACTATTATTAATTATTTAGTAGAGTTAACCGCATGTTGgag < 1:1950630/100‑2 (MQ=255) aCATACGTATTCTATTGTGATGCGAAGTATACATAGTTCTTTGTTTACTGAAAATACTATTATTAATTATTTAGTAGAGTTAACCGCATGTTGGATGata > 1:1812728/1‑100 (MQ=255) | ACACTGAGTGATATGGCAGGAAATCAACGTTACGTCTTTCTAGATCATAACAATCAATTTGTTGCAGATCAAAATGCGAAACATACGTATTCTAATGTGATGCGAAGTATACATAGTTCTTTGTTTACTGAAAATACTATTATTAATTATTTAGTAGAGTTAACCGCATGTTGGATGATA > NC_007793/1499015‑1499194 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |