Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,495,206	T→C	E1079G (GAA→GGA)	SAUSA300_RS07265 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,495,206	0	T	C	100.0%	76.7 / NA	30	E1079G (GAA→GGA)	SAUSA300_RS07265	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (0/30);  total (0/30)

ACTTTCAAATTGCGATTCAGCTTCTACAGCCATTTGCTTTACATATAATTCAAGCTGTTGCCTCAATAATCCCATTTGTGGTTGTAATAATTCTAACGTACTTTGACAAATTGATTCATGTATATCTCTTTGCCCATTTGGATTCAAAATTTTACGTTTTGTTAATTGTTTAGGCAATGCATTTAGC  >  NC_007793/1495115‑1495301                                                                                            |                                                                                                aCTTTCAAATTGCGATTCAGCTTCTACAGCCATTTGCTTTACATATAATTCAAGCTGTTGCCTCAATAATCCCATTTGTGGTTGTAATAATCCTAACGTa                                                                                         <  1:1703926/100‑1 (MQ=255)   tttCAAATTGCGATTCAGCTTCTACAGCCATTTGCTTTACATATAATTCAAGCTGTTGCCTCAATAATCCCATTTGTGGTTGTAATAATCCTAACGTAca                                                                                       <  1:5104768/100‑2 (MQ=255)   tttCAAATTGCGATTCAGCTTCTACAGCCATTTGCTTTACATATAATTCAAGCTGTTGCCTCAATAATCCCATTTGTGGTTGTAATAATCCTAACGTACt                                                                                       <  1:252620/100‑1 (MQ=255)   tttCAAATTGCGATTCAGCTTCTACAGCCATTTGCTTTACATATAATTCAAGCTGTTGCCTCAATAATCCCATTTGTGGTTGTAATAATCCTAACGTACt                                                                                       <  1:410275/100‑1 (MQ=255)   tttCAAATTGCGATTCAGCTTCTACAGCCATTTGCTTTACATATAATTCAAGCTGTTGACTCAATAATCCCATTTGTGGTTGTAATAATCCTAACGTACt                                                                                       <  1:906651/100‑1 (MQ=255)      cAAATTGCGATTCAGCTTCTACAGCCATTTGCTTTACATATAATTCAAGCTGTTGCCTCAATAATCCCATTTGTGGTTGTAATAATCCTAACGTACTTTg                                                                                    <  1:6294255/100‑1 (MQ=255)      cAAATTGCGATTCAGCTTCTACAGCCATTTGCTTTACATATAATTCAAGCTGTTGCCTCAATAATCCCATTTGTGGTTGTAATAATCCTAACGTACTTTg                                                                                    <  1:2884873/100‑1 (MQ=255)       aaaTTGCGATTCAGCTTCTACAGCCATTTGCTTTACATATAATTCAAGCTGTTGCCTCAATAATCCCATTTGTGGTTGTAAAAATCCTAACGTACTTTgg                                                                                   <  1:2013788/100‑2 (MQ=255)                       tCTACAGCCATTTGCTTTACATATAATTCAAGCTGTTGCCTCAATAATCCCATTTGTGGTTGTAATAATCCTAACGTACTTTGACAAATTGATTCATGtc                                                                   <  1:339147/100‑2 (MQ=255)                                   tGCTTTACATATAATTCAAGCTGTTGCCTCAATAATCCCATTTGTGGTTGTAATAATCCTAACGTACTTTGACAAATTGATTCATGTATATCTCTTTGcc                                                       <  1:946135/100‑1 (MQ=255)                                    gCTTTACATATAATTCAAGCTGTTGCCTCAATAATCCCATTTGTGGTTGTAATAATCCTAACGTTCTTTGACAAATTGATTCATGTATATCTCTTTGccc                                                      <  1:317241/100‑1 (MQ=255)                                    gCTTTACATATAATTCAAGCTGTTGCCTCAATAATCCCATTTGTGGTTGTAATAATCCTAACGTACTTTGACAAATTGATTCATGTATATCTCTTTGccc                                                      <  1:3993350/100‑1 (MQ=255)                                    gCTTTACATATAATTCAAGCTGTTGCCTCAATAATCCCATTTGTGGTTGTAATAATCCTAACGTACTTTGACAAATTGATTCATGTATATCTCTTTGccc                                                      <  1:6383939/100‑1 (MQ=255)                                    gCTTTACATATAATTCAAGCTGTTGCCTCAATAATCCCATTTGTGGTTGTAATAATCCTAACGTACTTTGACAAATTGATTCATGTATATCTCTTTGccc                                                      <  1:1486792/100‑1 (MQ=255)                                       ttACATATAATTCAAGCTGTTGCCTCAATAATCCCATTTGTGGTTGTAATAATCCTAACGTACTCTGACAAATTGATTCATGTATATCTCTTTGCCCAtt                                                   <  1:5337255/100‑1 (MQ=255)                                        tACATATAATTCAAGCTGTTGCCTCAATAATCCCATTTGTGGTTGTAATAATCCTAACGTACTTTGACAAATTGATTCATGTATATCTCTTTGCCCAtta                                                  <  1:5935303/100‑2 (MQ=255)                                               aaTTCAAGCTGTTGCCTCAATAATCCCATTTGTGGTTGTAATAATCCTAACGTACTTTGACAAATTGATTCATGTATATCTCTTTGCCCATTTGGATTCa                                           <  1:6241842/100‑1 (MQ=255)                                                                        ccATTTGTGGTTGTAATAATCCTAACGTACTTTGACAAATTGATTCATGTATATCTCTTTGCCCATTTGGATTCAAAATTTTACGTTTTGTTAATTGttt                  <  1:1067974/100‑1 (MQ=255)                                                                        atcttcgtgGTTGTAATAATCCTAACGTACTTTGACAAATTGATTCATGTATATCTCTTTGCCCATTTGGATTCAAAATTTTACGTTTTGTTAATTGttt                  <  1:2923386/94‑1 (MQ=255)                                                                            ttGTGGTTGTAATAATCCTAACGTACTTTGACAAATTGATTCATGTATAGCTCTTTGCCCATTTGGATTCAAAATTTTACGTTTTGTTAATTGTTTAGGc              <  1:6105220/100‑1 (MQ=255)                                                                               tgGTTGTAATAATCCTAACGTACTTTGACAAATTGATTCATGTATATCTCTTTGCCCATTTGGATTCAAAATTTTACGTTTTGTTAATTGTTTAGGCagt           <  1:5057800/100‑3 (MQ=255)                                                                                     taataaTCCTAACGTACTTTGACAAATTGATTCATGTATATCTCTTTGCCCATTTGGATTCAAAATTTTACGTTTTGTTAATTGTTTAGGCAATGCAttt     <  1:6245564/100‑1 (MQ=255)                                                                                        taatCCTAACGTACTTTGACAAATTGATTCATGTATATCTCTTTGCCCATTTGGATTCAAAATTTTACGTTTTGTTAATTGTTTAGGCAATGCATTTAGc  <  1:5992/100‑1 (MQ=255)                                                                                        taatCCTAACGTACTTTGACAAATTGATTCATGTATATCTCTTTGCCCATTTGGATTCAAAATTTTACGTTTTGTTAATTGTTTAGGCAATGCATTTAGc  <  1:6206785/100‑1 (MQ=255)                                                                                        taatCCTAACGTACTTTGACAAATTGATTCATGTATATCTCTTTGCCCATTTGGATTCAAAATTTTACGTTTTGTTAATTGTTTAGGCAATGCATTTAGc  <  1:5484609/100‑1 (MQ=255)                                                                                        taatCCTAACGTACTTTGACAAATTGATTCATGTATATCTCTTTGCCCATTTGGATTCAAAATTTTACGTTTTGTTAATTGTTTAGGCAATGCATTTAGc  <  1:5036031/100‑1 (MQ=255)                                                                                        taatCCTAACGTACTTTGACAAATTGATTCATGTATATCTCTTTGCCCATTTGGATTCAAAATTTTACGTTTTGTTAATTGTTTAGGCAATGCATTTAGc  <  1:6611356/100‑1 (MQ=255)                                                                                        taatCCTAACGTACTTTGACAAATTGATTCATGTATATCTCTTTGCCCATTTGGATTCAAAATTTTACGTTTTGTTAATTGTTTAGGCAATGCATTTAGc  <  1:1331588/100‑1 (MQ=255)                                                                                        taatCCTAACGTACTTTGACAAATTGATTCATGTATATCTCTTTGCCCATTTGGATTAAAAATTTTACGTTTTGTTAATTGTTTAGGCAATGCATTTAGc  <  1:3022325/100‑1 (MQ=255)                                                                                        taatCCTAACGTACCTTGACAAATTGATTCATGTATATCTCTTTGCCCATTTGGATTCAAAATTTTACGTTTTGTTAATTGTTTAGGCAATGCATTTAGc  <  1:4858381/100‑1 (MQ=255)                                                                                            |                                                                                                ACTTTCAAATTGCGATTCAGCTTCTACAGCCATTTGCTTTACATATAATTCAAGCTGTTGCCTCAATAATCCCATTTGTGGTTGTAATAATTCTAACGTACTTTGACAAATTGATTCATGTATATCTCTTTGCCCATTTGGATTCAAAATTTTACGTTTTGTTAATTGTTTAGGCAATGCATTTAGC  >  NC_007793/1495115‑1495301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑