Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,490,274	T→C	L357L (TTA→TTG)	SAUSA300_RS07245 ←	amino acid permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,490,274	0	T	C	100.0%	63.8 / NA	24	L357L (TTA→TTG)	SAUSA300_RS07245	amino acid permease
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (0/24);  total (0/24)

CGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATATTTGTAATTGTATCAAATGCTCCCATTGACATCATGATGATAGCGATTATAAGTTGTATAATTGCGCCAAACCATGGTGCGCC  >  NC_007793/1490178‑1490363                                                                                                 |                                                                                          cGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATCaaa                                                                                        <  1:5336219/100‑2 (MQ=255)   gTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATCAACat                                                                                      <  1:5658288/100‑1 (MQ=255)         tataTGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATCAACATATTTGt                                                                                <  1:5288753/100‑1 (MQ=255)                    tCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATCAACATATTTGTAATTGTATCaa                                                                     <  1:6433203/100‑1 (MQ=255)                    tCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATCAACATATTTGTAATTGTATCaa                                                                     <  1:6005292/100‑1 (MQ=255)                    tCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATCAACATATTTGTAATTGTATCaa                                                                     <  1:5951192/100‑1 (MQ=255)                    tCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATCAACATATTTGTAATTGTATCaa                                                                     <  1:5120103/100‑1 (MQ=255)                     ccATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATCAACATATTTGTAATTGTATCaaa                                                                    <  1:2712290/100‑1 (MQ=255)                        tatTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATCAACATATTTGTAATTGTATCAAATGc                                                                 <  1:165205/100‑1 (MQ=255)                         atTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATCAACATATTTGTAATTGTATCAAATGcc                                                                <  1:2074690/100‑2 (MQ=255)                            tGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATCAACATATTTGTAATTGTATCAAATGCTccc                                                             <  1:720610/100‑1 (MQ=255)                             ggTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATCAACATATTTGTAATTGTATCAAATGCTCCCa                                                            <  1:4436148/100‑1 (MQ=255)                               ttCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATCAACATATTTGTAATTGTATCAAATGCTTCCAtt                                                          <  1:2730910/100‑1 (MQ=255)                                tCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATCAACATATTTGTAATTGTATCAAATGCTCCCATTg                                                         <  1:106317/100‑1 (MQ=255)                                ccgcGTTTTCTTAAAATTATTACCGCAACAAATCACGTACAATAGAACAACCAAAAAACAAAGATCAACATATTTGTAATTGTATCAAATGCTCCCAGTg                                                         <  1:2761504/97‑1 (MQ=255)                                     ttttCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATCAACATATTTGGAATTGTATCAAATGCTCCCATTGAcatc                                                    <  1:3027650/100‑1 (MQ=255)                                      tttCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATCAACATATTTGTAATTGTATCAAATGCTCCCATTGAcatca                                                   <  1:384898/100‑1 (MQ=255)                                                      ccGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATCAACATATTTGTAATTGTATCAAATGCTCCCATTGACATCATGATGATAGCGATtat                                   <  1:2167900/100‑1 (MQ=255)                                                                               caacCAAATAACAAAGATCAACATATTTGTAATTGTATCAAATGCTCCCATTGACATCATGATGATAGCGATTATAAGTTGTATAATTGCGCCAAACCag          <  1:1345621/100‑2 (MQ=255)                                                                                      aTAACAAAGATCAATATAGTTGTAATTGTATCAAATGCTCCCATTGACATCATGATGATAGCGATTATAAGTTGTATAATTGCGCCAAACCATGGTgcgt   <  1:4919918/100‑2 (MQ=255)                                                                                       tAACAAAGATCAACATATTTGTAATTGTATCAAATGCTCCCATTGACATCATGATGATAGCGATTATAAGTTGTATAATTGCGCCAAACCATGGTGCGcc  <  1:3904769/100‑1 (MQ=255)                                                                                       tAACAAAGATCAACATATTTGTAATTGTATCAAATGCTCCCATTGACATCATGATGATAGCGATTATAAGTTGTATAATTGCGCCAAACCATGGTGCGcc  <  1:5497519/100‑1 (MQ=255)                                                                                       tAACAAAGATCAACATATTTGTAATTGTATCAAATGCTCCCATTGACATCATGATGATAGCGATTATAAGTTGTATAATTGCGCCAAACCATGGTGCGcc  <  1:2793976/100‑1 (MQ=255)                                                                                       tAACAAAGATCAACATATTTGTAATTGTATCAAATGCTCCCATTGACATCATGATGATAGCGATTATAAGTTGTATAATTGCGCCAAACCATGGTGCGcc  <  1:5857012/100‑1 (MQ=255)                                                                                                 |                                                                                          CGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATATTTGTAATTGTATCAAATGCTCCCATTGACATCATGATGATAGCGATTATAAGTTGTATAATTGCGCCAAACCATGGTGCGCC  >  NC_007793/1490178‑1490363

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑