Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,462,539	G→A	A8513V (GCA→GTA)	SAUSA300_RS07235 ←	hyperosmolarity resistance protein Ebh

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,462,539	0	G	A	100.0%	153.1 / NA	55	A8513V (GCA→GTA)	SAUSA300_RS07235	hyperosmolarity resistance protein Ebh
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (3/52);  total (3/52)

TCCATATGTTTTTGTGCATTGTTTAGATGTGCTAATTGACTAACCGTTTGCTTAGCATGATCTTTATCATTTTGTAACTTCACATCACCATGTAATGCTGCTTTCGTTGTATTCACAGTTGTCGCTGCTTGATTAATCGCATTATTATCTAATGTTGCAGTTTGTTCATTGATAATATTATTTGCGGCTTGA  >  NC_007793/1462443‑1462634                                                                                                 |                                                                                                ttccgatctttttGTGCATTGTTTAGATGTGCTAATTGACTAACCGTTTGCTTAGCATGATCTTTATCATTTTGTAACTTCACATCACCATGTAAtactg                                                                                              <  1:51458/92‑1 (MQ=255)   gatctGTTTTTGCGCATTGTTTAGATGTGCTAATTGACTAACCGTTTGCTTAGCATGATCTTTATCATTTTGTAACTTCACATCACCATGTAATactgct                                                                                            <  1:5551672/96‑1 (MQ=255)     tctGTTTTTGTGCATTGTTTAGATGTGCTAATTGACTGACCGTTTGCTTAGCATGATCTTTATCATTTTGTAACTTCACATCACCATGTAATACTGCttt                                                                                          <  1:358831/98‑1 (MQ=255)       tGTTTTTGTGCATTGTTTAGATGTGCTAATTGACTAACCGTTTGCTTAGCATGATCTTTATCATTTTGTAACTTCACATCACCATGTAATACTGCTTTCg                                                                                        <  1:2884704/100‑1 (MQ=255)       tGTTTTTGTGCATTGTTTAGATGTGCTAATTGACTAACCGTTTGCTTAGCATGATCTTTATCATTTTGTAACTTCACATCACCATGTAATACTGCTTTCg                                                                                        <  1:2257879/100‑1 (MQ=255)            ttGTGCATTGTTTAGATGTGCTAATTGACTAACCGTTTGCTTAGCATGATCTTTATCATTTTGTAACTTCACATCACCATGTAATACTGCTTTCGTTGTa                                                                                   <  1:276702/100‑1 (MQ=255)            atgtgCATTGTTTAGCTGTGCTAATTGACTAACCGTTTGCTTAGCATGATCTTTATCATTTTGTAACTTCACATCACCATGTAATACTGCTTTCGTTGTa                                                                                   >  1:2881387/2‑100 (MQ=255)               tgCATTGTTTAGATGTGCTAATTGACTAACCGTTTGCTTAGCATGATCTTTATCATTTTGTAACTTCACATCACCATGTAATACTGCTTTCGTTGTATTc                                                                                <  1:6057865/100‑1 (MQ=255)                gCATTGTTTAGATGTGCTAATTGACTAACCGTTTGCTTAGCATGATCTTTATCATTTTGTAACTTCACATCACCATGTAATACTGCTTTCGTTGTATTcg                                                                               <  1:2298838/100‑2 (MQ=255)                      tttAGATGTGCTAAGTGACTAACCGTTTGCTTAGCATGATCTTTATCATTTTGTAACTTCACATCACCATGTAATACTGCTTTCGTTGTATTCACAGTTg                                                                         <  1:4979774/100‑1 (MQ=255)                          gATGTGCTAATTGACTAACCGTTTGCTTAGCATGATCTTTATCATTTTGTAACTTCACATCACCATGTAATACTGCTTTCGTTGTATTCACAGTTGTCgg                                                                     <  1:2744241/100‑2 (MQ=255)                           aTGTGCTAATTGACTAACCGTTTGCTTAGCATGATCTTTATCATTTTGTAACTTCACATCACCATGTAATACTGCTTTCGTTGTATTCACAGTTGTCgct                                                                    <  1:155654/100‑1 (MQ=255)                           aTGTGCTAATTGACTAACCGTTTGCTTAGCATGATCTTTATCATTTTGTAACTTCACATCACCATGTAATACTGCTTTCGTTGTATTCACAGTTGTCgcc                                                                    <  1:4338357/100‑2 (MQ=255)                              tgCTAATTGACTAACCGTTTGCTTAGCATGATCTTTATCATTTTGTAACTTCACATCACCATGTAATACTGCTTTCGTTGTATTCACAGTTGTCgctgag                                                                 <  1:3213587/100‑3 (MQ=255)                              ctcttccgatcTAACCGTTTGCTTAGCATGATCTTTATCATTTTGTAACTTCACATCACCATGTAATACTGCTTTCGTTGTATTCACAGTTGTCgctgct                                                                 <  1:4765739/90‑1 (MQ=255)                               tcttccgatcTAACCGCTTGCTTAGCATGATCTTTATCATTTTGTAACTTCACATCACCATGTAATACTGCTTTCGTTGTATTCACAGTTGTCGCTGCtt                                                                <  1:4294492/91‑1 (MQ=255)                                cttccgatcTAACCGCTTGCTTAGCATGATCTTTATCATTTTGTAACTTCACATCACCATGTAATACTGCTTTCGTTGTATTCACAGTTGTCGCTGCTTg                                                               <  1:4127406/92‑1 (MQ=255)                                cTAATTGACTAACCGTTTGCTTAGCATGATCTTTATCATTTTGTAACTTCACATCACCATGTAATACTGCTTTCGTTGTATTCACAGTTGTCGCTGCTTg                                                               <  1:482557/100‑1 (MQ=255)                                cTAATTGACTAACCGTTTGCTTAGCATGATCTTTATCATTTTGTAACTTCACATCACCATGTAATACTGCTTTCGTTGTATTCACAGTTGTCGCTGCTTg                                                               <  1:3146998/100‑1 (MQ=255)                                 ttccgatcTAACCATTTGCTTAGCATGATCTTTATCATTTTGTAACTACACATCACCATGTAATACTGCTTTCGTTGTAGTCACAGTTGTCGCTGCTTGa                                                              <  1:3584154/93‑1 (MQ=255)                                   aTTGACTAACCGTTTGCTTAGCATGATCTTTATCATTTTGTAACTTCACATCACCATGTAATACTGCTTTCGTTGTATTCACAGTTGTCGCTGCTTGAtt                                                            <  1:6059643/100‑1 (MQ=255)                                    cgatcTAACCGTTTGCTTAGCATGATCTTTATCATTTTGTAACTTCACATCACCATGTAATACTGCTTTCGTTGTATTCACAGTTGTCGCTGCTTGATTa                                                           <  1:3062569/96‑1 (MQ=255)                                       ctccaCCGTTTGCTTAGCATGATCTTTATCATTTTGTAACTTCACATCACCATGTAATACTGCTTTCGTTGTATTCACAGTTGTCGCTGCTTGATTaagc                                                        <  1:6312139/96‑3 (MQ=255)                                        cTAACCGTTTGCTTAGCATGATCTTTATCCTTTTGTAACTTCCCATCCCCATGTAATACTGCTTTCGTTGTATTCACAGTTGTCGCTGCTTGATTaagaa                                                       <  1:5677304/100‑4 (MQ=255)                                                    ttAGCATGATCTTTATCATTTTGTAACTTCACATCACCATGTAATACTGCTTTCGTTGTATTCACAGTTGTCGCTGCTTGATTAATCGCATTATTATCTa                                           <  1:6587070/100‑1 (MQ=255)                                                     tAGCATGATCTTTATCATTTTGTAACTTCACATCACCATGTAATACTGCTTTCGTTGTATTCACAGCTGTCGCTGCTTGATTAATCGCATTATTATCTaa                                          >  1:3456057/1‑100 (MQ=255)                                                          tGATCTTTATCATTTTGTAACTTCACATCACCATGTAATACTGCTTTCGTTGTATTCACAGTTGTCGCTGCTTGATTAATCGCATTATTATCTAATGTTg                                     <  1:4297925/100‑1 (MQ=255)                                                          cgATCTTTGT‑ATTTTGTAACTTCACATCACCATGTAATACTGCTTTCGTTGTATTCACAGTTGTCGCTGCTTGATTAATCGCATTATTATCTAATGTTGc                                    <  1:2977288/99‑1 (MQ=255)                                                          cgATCTTTATC‑GTTTGTAACTTCACATCACCATGTAATACTGCTTTCGTTGTATTCACAGTTGTCGCTGCTTGATTAATCGCATTATTATCTAATGTTGc                                    <  1:4163906/99‑1 (MQ=255)                                                          cgATCTTTATC‑CTTGGTAACTTCACATCACCATGTAATACTGCTTTCGTTGTATTCACAGTTGTCGCTGCTTGATTAATCGCATTATTATCTAATGTTGc                                    <  1:4853849/99‑1 (MQ=255)                                                           gATCTTTATCTTTTTGTAACTTCACATCACCATGTAATACTGCTTTCGTTGTATTCAAAGTTGTCGCTGCTTGATTAATCGCATTATTATCTAATGTTGc                                    <  1:6225670/100‑1 (MQ=255)                                                           gATCTTTATCATTTTGTAACTTCACATCACCATGTAATACTGCTTTCGTTGTATTCACAGTTGTCGCTGCTTGATTAATCGCATTATTATCTAATGTTGc                                    <  1:3520700/100‑1 (MQ=255)                                                           gATCTTTATCATTTTGTAACTTCACATCACCATGTAATACTGCTTTCGTTGTATTCACAGTTGTCGCTGCTTGATTAATCGCATTATTATCTAATGTTGc                                    <  1:3481404/100‑1 (MQ=255)                                                           gATCTTTATCATTTTGTAACTTCACATCACCATGTAATACTGCTTTCGTTGTATTCACAGTTGTCGCTGCTTGATTAATCGCATTATTATCTAATGTTGc                                    <  1:5387015/100‑1 (MQ=255)                                                           gATCTTTATCATTTTGTAACTTCACATCACCATGTAATACTGCTTTCGTTGTATTCACAGTTGTCGCTGCTTGATTAATCGCATTATTATCTAATGTTGc                                    <  1:1959159/100‑1 (MQ=255)                                                           gATCTTTATCATTTTGTAACTTCACATCACCATGTAATACTGCTTTCGTTGTATTCACAGTTGTCGCTGCTTGATTAATCGCATTATTATCTAATGTTGc                                    <  1:4056654/100‑1 (MQ=255)                                                           gATCTTTATCATTTTGTAACTTCACATCACCATGTAATACTGCTTTCGTTGTATTCACAGTTGTCGCTGCTTGATTAATCGCATTATTATCTAATGTTGc                                    <  1:6091332/100‑1 (MQ=255)                                                           gATCTTTATCATTTTGTAACTTCACATCACCATGTAATACTGCTTTCGTTGTATTCACAGTTGTCGCTGCTTGATTAATCGCATTATTATCTAATGTTGc                                    <  1:3360709/100‑1 (MQ=255)                                                           gATCTTTATCATTTTGTAACTTCACATCACCATGTAATACTGCTTTCGTTGTATTCACAGTTGTCGCTGCTTGATTAATCGCATTATTATCTAATGTTGc                                    <  1:2857953/100‑1 (MQ=255)                                                           gATCTTTATCATTTTGTAACTTCACATCACCATGTAATACTGCTTTCGTTGTATTCACAGTTGTCGCTGCTTGATTAATCGCATTATTATCTAATGTTGc                                    <  1:1368190/100‑1 (MQ=255)                                                           gATCTTTATCATTTTGTAACTTCACATCACCATGTAATACTGCTTTCGTTGTATTCACAGTTGTCGCTGCTTGATTAATCGCATTATTATCTAATGTTGc                                    <  1:1342978/100‑1 (MQ=255)                                                           gATCTTTATCATTTTGTAACTTCACATCACCATGTAATACTGCTTTCGTTGGATTCACAGTTGTCGCTGCTTGATTAATCGCATTATTATCTAATGTTGc                                    <  1:5667996/100‑1 (MQ=255)                                                           gATCTTTACCATGTTGTAACTTCACATCACCATGTAATACTGCTTTCGTTGTATTCACAGTTGTCGCTGCTTGATTAATCGCATTATTATCTAATGTTGc                                    <  1:873043/100‑1 (MQ=255)                                                           gATCTCTATCTTTTTGTAACTTCACATCACCATGTAATACTGCTTTCGTTGTATTCACAGTTGTCGCTGCTTGATTAATCGCATTATTATCTAATGTTGc                                    <  1:2148698/100‑1 (MQ=255)                                                            aTCTTTATCATTTTGTAACTTCACATCACCATGTAATACTGCTTTCGTTGTATTCACAGTTGTCGCTGCTTGATTAATCGCATTATTATCTAATGTTGCa                                   <  1:3897865/100‑1 (MQ=255)                                                            aTCTTTATCATTTTGTAACTTCACATCACCATGTAATACTGCTTTCGTTGTATTCACAGTTGTCGCTGCTTGATTAATCGCATTATTATCTAATGTTGCa                                   <  1:2767188/100‑1 (MQ=255)                                                             tCTTTATCATTTTGTAACTTCACATCACCATGTAATACTGCTTTCGTTGTATTCACAGTTGTCGCTGCTTGATTAATCGCATTATTATCTAATGTTGCAg                                  <  1:5551244/100‑1 (MQ=255)                                                                ttATCATTTTGTAACTTCACATCACCATGTAATACTGCTTTCGTTGTATTCACAGTTGTCGCTGCTTGATTAATCGCATTATTATCTAATGTTGCAGttt                               >  1:1857752/1‑100 (MQ=255)                                                                    cactttGTAACTTCACATCACCATGTAATACTGCTTTCGTTGTATTCACAGTTGTCGCTGCTTGATTAATCGCATTATTATCTAATGTTGCAGTTTGTTc                           <  1:117122/97‑1 (MQ=255)                                                                    cATTTTGTAACTTCACATCACCATGTAATACTGCTTTCGTTGTATTCACAGTTGTCGCTGCTTGATTAATCGCATTATTGTCTAATGTTGCAGTTTGTTc                           <  1:1234379/100‑1 (MQ=255)                                                                    cATTTTGTAACTTCACATCACCATGTAATACTGCTTTCGTTGTATTCACAGTTGTCGCTGCTTGATTAATCGCATTATTATCTAATGTTGCAGTTTGTTc                           <  1:2288411/100‑1 (MQ=255)                                                                    cATTTTGTAACTTCACATCACCATGTAATACTGCTTTCGTTGTATTCACAGTTGTCGCTGCTTGATTAATCGCATTATTATCTAATGTTGCAGTTTGTTc                           <  1:2188753/100‑1 (MQ=255)                                                                                           tGTAATACTGCTTTCGTTGTATTCACAGTTGTCGCTGCTTGATTAATCGCATTATTATCTAATGTTGCAGTTTGTTCATTGATAATATTATTTGCGGCtt    <  1:6110086/100‑1 (MQ=255)                                                                                            gTAATACTGCTTTCGTTGTATTCACAGTTGTCGCTGCTTGATTAATCGCATTATTATCTAATGTTGCAGTTTGTTCATTGATAATATTATTTGCGGCTTg   <  1:3747086/100‑1 (MQ=255)                                                                                            gTAATACTGCTTTCGTTGTATTCACAGTTGTCGCTGCTTGATTAATCGCATTATTATCTAATGTTGCAGTTTGTTCATTGATAATATTATTTGCGGCTTg   <  1:1393596/100‑1 (MQ=255)                                                                                             tAATACTGCTTTCGTTGTATTCACAGTTGTCGCTGCTTGATTAATCGCATTATTATCTAATGTTGCAGTTTGTTCATTGATAATATTATTTGCGGCTTGa  <  1:1797388/100‑1 (MQ=255)                                                                                             tAATACTGCTTTCGTTGTATTCACAGTTGTCGCTGCTTGATTAATCGCATTATTATCTAATGGTGCAGTTTGTTCATTGATAATATTATTTGCGGCTTgc  <  1:3352520/100‑2 (MQ=255)                                                                                                 |                                                                                                TCCATATGTTTTTGTGCATTGTTTAGATGTGCTAATTGACTAACCGTTTGCTTAGCATGATCTTTATCATTTTGTAACTTCACATCACCATGTAATGCTGCTTTCGTTGTATTCACAGTTGTCGCTGCTTGATTAATCGCATTATTATCTAATGTTGCAGTTTGTTCATTGATAATATTATTTGCGGCTTGA  >  NC_007793/1462443‑1462634

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑