Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,453,525	A→G	V281A (GTT→GCT)	SAUSA300_RS07200 ←	virulence factor C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,453,525	0	A	G	100.0%	119.5 / NA	47	V281A (GTT→GCT)	SAUSA300_RS07200	virulence factor C
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (6/41);  total (6/41)

GTGGATCATCTAATAGTAGCACCATTTCTGGTAGTGCCTCTGGATACCCTAAATCGCTTATGCAATCCCCTGCTGTTCTTCTTACAACAGGACTTTTATCACGAAGCCCCTTATATAAATACGGTAAAATTTCTTTACTTTCAATCATACCTAATAATACAATCGCTTGACGTCTAACCGGT  >  NC_007793/1453439‑1453620                                                                                       |                                                                                                gtgGATCATCTAATAGTAGCACCATTTCTGGTAGTGCCTCTGGATACCCTAAATCGCTTATGCAATCCCCTGCTGTTCTTCTTACAGCAGGACTTTTATc                                                                                    <  1:4069571/100‑1 (MQ=255) gtgGATCATCTAATAGTAGCACCATTTCTGGTAGTGCCTCTGGATACCCTAAATCGCTTATGCAATCCCCTGCTGTTCTTCTTACAGCAGGACTTTTATc                                                                                    <  1:3882687/100‑1 (MQ=255)   ggATCATCTAATAGTAGCACCATTTCTGGTAGTGCCTCTGGATACCCTAAATCGCTTATGCAATCCCCTGCTGTTCTTCTTACAGCAGGACTTTTATCAc                                                                                  <  1:387192/100‑1 (MQ=255)    tccgatcTAATAGTAGCACCATTTCTGGTAGTGCCTCTGGATACCCTAAATCGCTTATGCAATCCCCTGCTGTTCTTCTTACAGCAGGACTTTTATCACg                                                                                 <  1:4287884/96‑1 (MQ=255)    gATCATCTAATAGTAGCCCCCTTTCTGGTAGTGCCTCTGGATACCCTAAATCGCTTATGCAATCCCCTGCTGTTCTTCTTACAGCAGGCCTTTTATCACg                                                                                 <  1:1385849/100‑1 (MQ=255)    gATCATCTAATAGTAGCACCATTTCTGGTAGTGCCTCTGGATACCCTAAATCGCTTATGCAATCCCCTGCTGTTCTTCTTACAGCAGGACTTTTATCAcc                                                                                 <  1:2695726/100‑2 (MQ=255)    gATCATCTAATAGTAGCACCATTTCTGGTAGTGCCTCTGGATACCCTAAATCGCTTATGCAATCCCCTGCTGTTCTTCTTACAGCAGGACTTTTATCACg                                                                                 <  1:4697721/100‑1 (MQ=255)    gATCATCTAATAGTAGCACCATTTCTGGTAGTGCCTCTGGATACCCTAAATCGCTTATGCAATCCCCTGCTGTTCTTCTTACAGCAGGACTTTTATCACg                                                                                 <  1:1515005/100‑1 (MQ=255)    gATCATCTAATAGTAGCACCATTTCTGGTAGTGCCTCTGGATACCCTAAATCGCTTATGCAATCCCCTGCTGTTCTTCTTACAGCAGGACTTTTATCACg                                                                                 <  1:2088186/100‑1 (MQ=255)    gATCATCTAATAGTAGCACCATTTCTGGTAGTGCCTCTGGATACCCTAAATCGCTTATGCAATCCCCTGCTGTTCTTCTTACAGCAGGACTTTTATCACg                                                                                 <  1:2411329/100‑1 (MQ=255)        ttcTAATAGTAGCACCATTTCTGGTAGTGCCTCTGGATACCCTAAATCGCTTATGCAATCCCCTGCTGTTCTTCTTACAGCAGGACTTTTATCTCGagag                                                                             <  1:4617920/99‑4 (MQ=255)            aaTAGTAGCACCATTTCTGGTAGTGCCTCTGGATACCCTAAATCGCTTATGCAATCCCCTGCTGTTCTTCTTACAGCAGGACTTTTATTACGAAGCCCCt                                                                         >  1:3981756/1‑100 (MQ=255)            aaTAGTAGCACCATTTCTGGTAGTGCCTCTGGATACCCTAAATCGCTTATGCAATCCCCTGCTGTTCTTCTTACAGCAGGACTTTTATCACGAAGCCCCt                                                                         >  1:992313/1‑100 (MQ=255)            aaTAGTAGCACCATTTCTGGTAGTGCCTCTGGATACCCTAAATCGCTTATGCAATCCCCTGCTGTTCTTCTTACAGCAGGACTTTTATCACGAAGCCCCt                                                                         <  1:2535592/100‑1 (MQ=255)            aaTAGTAGCACCATTTCTGGTAGTGCCTCTGGATACCCTAAATCGCTTATGCAATCCCCTGCTGATCTTCTTACAGCAGGACTTTTATCACGAAGcccca                                                                         >  1:829250/1‑99 (MQ=255)             aTAGTAGCGCCATTTCTGGTAGTGCCTCTGGATACCCTAAATCGCTTATGCAATCCCCGGCTGTTCTTCTTACAGCAGGACTTTTATCACGAAGCCCCtg                                                                        <  1:882294/100‑2 (MQ=255)             aTAGTAGCACCATTTCTGGTAGTGCCTCTGGATACCCTAAATCGCTTATGCAATCCCCTGCTGTTCTTCTTACAGCAGGACTTTTATCACGAAGCCCCtt                                                                        <  1:867727/100‑1 (MQ=255)             aTAGTAGCACCATTTCTGGTAGTGCCTCTGGATACCCTAAATCGCTTATGCAATCCCCTGCTGTTCTTCTTACAGCAGGACTTTTATCACGAAGCCCCtt                                                                        <  1:375245/100‑1 (MQ=255)             aTAGTAGCACCATTTCTGGTAGTGCCTCTGGATACCCTAAATCGCTTATGCAATCCCCTGCTGTTCTTCTTACAGCAGGACTTTTATCACGAAGCCCCtt                                                                        <  1:2029761/100‑1 (MQ=255)                gtagCACCATTTCTGGTAGTGCCTCTGGATACCCTAAATCGCTTATGCAATCCCCGGCTGTTCTTCTTACAGCAGGACTTTTATCACGAAGCCCCttggc                                                                     <  1:6007053/100‑4 (MQ=255)                          ttCTGGTAGTGCCTCTGGATACCCTAAATCGCTTATGCAATCCCCTGCTGTTCTTCTTACAGCAGGACTTTTATCACGAAGCCCCTTATATAAATACGGt                                                           <  1:6632196/100‑1 (MQ=255)                           tCTGGTAGTGCCTCTGGATACCCTAAATCGCTTATGCAATCCCCTGCTGTTCTTCTTACAGCAGGACTTTTATCACGAAGCCCCTTATATAAATACGGTa                                                          <  1:1869618/100‑1 (MQ=255)                            cTGGTAGTGCCTCTGGATACCCTAAATCGCTTATGCAATCCCCTGCTGTTCTTCTTACAGCAGGACTTTTATCACGAAGCCCCTTATATAAATACGGTat                                                         <  1:916182/100‑2 (MQ=255)                            cTGGTAGTGCCTCTGGATACCCTAAATCGCTTATGCAATCCCCTGCTGTTCTTCTTACAGCAGGACTTTTATCACGAAGCCCCTTATATAAATACGGTaa                                                         <  1:3542434/100‑1 (MQ=255)                             tGGTAGTGCCTCCGGATACCCTAAATCGCTTATGCAATCCCCTGCTGTTCTTCTGACAGCAGGACTTTTATCACGAAGCCCCTTATATAAATACGGTaaa                                                        <  1:4423620/100‑1 (MQ=255)                                 aGTGCCTCTGGATACCCTAAATCGCTTATGCAATCCTCTGCTGTTCTTCTTACAGCAGGACTTTTATCACGAAGCCCCTTATATAAATACGGtcccatgt                                                    <  1:3485279/100‑8 (MQ=255)                                    gCCTCTGGATACCCTAAATCGCTTATGCAATCCCCTGCTGTTCTTCTTACAGCAGGACTTTTATTACGAAGCCCCTTATATAAATACGGTAAAATTTCtt                                                 <  1:1183301/100‑1 (MQ=255)                                          ggATACCCTAAATCGCTTATGCAATCCCCTGCTGTTCTTCTTACAGCAGGACTTTTATCACGAAGCCCCTTATATAAATACGGTAAAATTTCTTTACttt                                           >  1:6599826/1‑100 (MQ=255)                                                 cTAAATCGCTTATGCAATCCCCTGCTGTTCTTCTTACAGCAGGACTTTTATCACGAAGCCCCTTATATAAATACGGTAAAATTTCTTTACTTTCAATCAt                                    <  1:381465/100‑1 (MQ=255)                                                 cTAAATCGCTTATGCAATCCCCTGCTGTTCTTCTTACAGCAGGACTTTTATCACGAAGCCCCTTATATAAATACGGTAAAATTTCTTTACTTTCAATCAt                                    <  1:547413/100‑1 (MQ=255)                                                   aaaTCGCTTATGCAATCCCCTGCTGTTCTTCTTACAGCAGGACTTTTATCACGAAGCCCCTTATATAAATACGGTAAAATTTCTTTACTTTCAATCATAc                                  >  1:1676174/1‑100 (MQ=255)                                                     aTCGCTTATGCAATCCCCTGCTGTTCTTCTTACAGCAGGACTTTTATCACGAAGCCCCTTATATAAATACGGTAAAATTTCTTTACTTTCAATCATACCt                                <  1:488956/100‑1 (MQ=255)                                                      tCGCTTATGCAATCCCCTGCTGTTCTTCTTACAGCAGGACTTTTATCACGAAGCCCCTTATATAAATACGGTAAAATTTCTTTACTTTCAATCATACCta                               <  1:3318160/100‑1 (MQ=255)                                                      tCGCTTATGCAATCCCCTGCTGTTCTTCTTACAGCAGGACTTTTATCACGAAGCCCCTTATATAAATACGGTAAAATTTCTTTACTTTCAATCATACCta                               <  1:5467512/100‑1 (MQ=255)                                                      tCGCTTATGCAATCCCCTGCTGTTCTTCTTACAGCAGGACTTTTATCACGAAGCCCCTTATATAAATACGGTAAAATTTCTTTACTTTCAATCATACCta                               <  1:545791/100‑1 (MQ=255)                                                       cGCTTATGCAATCCCCTGCTGTTCTTCTTACAGCAGGACTTTTATCACGAAGCCCCTTATATAAATACGGTAAAATTTCTTTACTTTCAATCATACCtaa                              <  1:1112602/100‑1 (MQ=255)                                                           tATGCAATCCCCTGCTGTTCTTCTTACAGCAGGACTTTTATCACGAAGCCCCTTATATAAATACGGTAAAATTTCTTTACTTTCAATCATACCTaataat                          >  1:5224892/1‑100 (MQ=255)                                                             tGCAATCCCCTGCTGTTCTTCTTACAGCAGGACTTTTATCACGAAGCCCCTTATATAAATACGGTAAAATTTCTTTACTTTCAATCATACCTAATAATAc                        <  1:530503/100‑1 (MQ=255)                                                             tGCAATCCCCTGCTGTTCTTCTTACAGCAGGACTTTTATCACGAAGCCCCTTATATAAATACGGTAAAATTTCTTTACTTTCAATCATACCTAATAATAc                        <  1:2727050/100‑1 (MQ=255)                                                               cAATCCCCTGCTGTTCTTCTTACAGCAGGACTCTTATCACGAAGCCCCTTATATAAATACGGTAAAATTTCTTTACTTTCAATCATACCTAATAATACaa                      <  1:6490667/100‑1 (MQ=255)                                                                 aTCCCCTGCTGTTCTTCTTACAGCAGGACTTTTATCACGAAGCCCCTTATATAAATACGGTAAAATTTCTTTACTTTCAATCATACCTAATAATACAAta                    <  1:419311/100‑2 (MQ=255)                                                                     ccTGCTGTTCTTCTTACAGCAGGACTTTTATCACGAAGCCCCTTATATAAATACGGTAAAATTTCTTTACTTTCAATCATACCTAATAATACAATCGCta                <  1:1689046/100‑2 (MQ=255)                                                                          tgTTCTTCTTACAGCAGGACTTTTATCACGAAGCCCCTTATATAAATACGGTAAAATTTCTTTACTTTCAATCATACCTAATAATACAATCGCTTGACGt           <  1:2997940/100‑1 (MQ=255)                                                                             tcttctTACAGCAGGACTTTTATCACGAAGCCCCTTATATAAATACGGTAAAATTTCTTTACTTTCAATCATACCTAATAATACAATCGCTTGACGTCTa        <  1:2774761/100‑1 (MQ=255)                                                                              cttcttACAGCAGGACTTTTATCACGAAGCCCCTTATATAAATACGGTAAAATTTCTTTACTTTCAATCATACCTAATAATACAATCGCTTGACGTCTac       <  1:4399655/100‑2 (MQ=255)                                                                                 cttACAGCAGGACTTTTATCACGAAGCCCCTTATATAAATACGGTAAAATTTCTTTACTTTCAATCATACCTAATAATACAATCGCTTGACGTCTAACCg    <  1:4334517/100‑1 (MQ=255)                                                                                   tACAGCAGGACTTTTATCACGAAGCCCCTTATATAAATACGGTAAAATTTCTTTACTTTCAATCATACCTAATAATACAATCGCTTGACGTCTAACCGGt  <  1:1740137/100‑1 (MQ=255)                                                                                       |                                                                                                GTGGATCATCTAATAGTAGCACCATTTCTGGTAGTGCCTCTGGATACCCTAAATCGCTTATGCAATCCCCTGCTGTTCTTCTTACAACAGGACTTTTATCACGAAGCCCCTTATATAAATACGGTAAAATTTCTTTACTTTCAATCATACCTAATAATACAATCGCTTGACGTCTAACCGGT  >  NC_007793/1453439‑1453620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑