Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,433,527	C→T	G189D (GGT→GAT)	SAUSA300_RS07085 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,433,527	0	C	T	100.0%	50.0 / NA	20	G189D (GGT→GAT)	SAUSA300_RS07085	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (0/20);  total (0/20)

CGGATACTAGCGGCTTCTTCAGAAATTTGTCCCTGCTTAGACATAGTACGTAAATCTTCATTAAACTTAATAATTTGTTCGATTTGTTTATCACCTTGTAATAAACTTTGCTCTTTAATCACATTTTTTAAAATGTCCCCATCAATATAATCAACGTGAATAACAACAAAGCGATTTTTTAGTGCTT  >  NC_007793/1433434‑1433620                                                                                              |                                                                                              cGGATACTAGCGGCTTCTTCAGAAATTTGTCCCTGCTTAGACATAGTACGTAAATCTTCATTAAACTTAATAATTTGTTCGATTTGTTTATCATCTTGta                                                                                         <  1:1059619/100‑1 (MQ=255)             gCTTCTTCAGAAATTTGTCCCTGCTTAGACATAGTACGTAAATCTTCATTAAACTTAATAATTTGTTCGATTTGTTTATCATCTTGTAATAAACTTTGcc                                                                             <  1:2121149/100‑2 (MQ=255)                  ttcAGAAATTTGTCCCTGCTTAGACATAGTACGTAAATCTTCATTAAACTTAATAATTTGTTCGATTTGTTTATCATCTTGTAATAAACTTTGCTCTTTa                                                                        <  1:2581531/100‑1 (MQ=255)                         ccgatctccTGCTTAGACATAGTACGTAAATCTTCATTAAACTTAATAATTTGTTCGATTTGTTTATCATCTTGTAATAAACTTTGCTCTTTAATCACAt                                                                 <  1:1293800/93‑1 (MQ=255)                         aTTTGTCCCTGCTTAGACATAGTACGTAAATCTTCATTAAACTTAATAATTTGTTCGATTTGTTTATCATCTTGTAATAAACTTTGCTCTTTAATCACAt                                                                 <  1:5888554/100‑1 (MQ=255)                          tttGTCCCTGCTTAGACATAGTACGTAAATCTTCATTAAACTTAATAATTTGTTCGATTTGTTTATCATCTTGTAATAAACTTTGCTCTTTAATCACAtt                                                                <  1:148307/100‑1 (MQ=255)                          tttGTCCCTGCTTAGACATAGTACGTAAATCTTCATTAAACTTAATAATTTGTTCGATTTGTTTATCATCTTGTAATAAACTTTGCTCTTTAATCACAtc                                                                <  1:1674858/100‑2 (MQ=255)                                          cATAGTACGTAAATCTTCATTAAACTTAATAATTTGTTCGATTTGTTTATCATCTTGTAATAAACTTTGCTCTTTAATCACATTTTTTAAAATGTCCCCa                                                <  1:290449/100‑1 (MQ=255)                                            tAGTACGTAAATCTTCATTAAACTTAATAATTTGTTCGATTTGTTTATCATCTTGTAATAAACTTTGCTCTTTAATCACATTTTTTAAAATGTCCCCAtg                                              <  1:2773600/100‑2 (MQ=255)                                                 cGTAAATCTTCATTAAACTTAATAATTTGTTCGATTTGTTTATCATCTTGTAATAAACTTTGCTCTTTAATCACATTTTTTAAAATGTCCCCATcggctg                                         <  1:280096/100‑6 (MQ=255)                                                      aTCTTCATTAAACTTAATAATTTGTTCGATTTGTTTATCATCTTGTAATAAACTTTGCTCTTTAATCACATTTTTTAAAATGTCCCCATCAATATAATCa                                    <  1:6348904/100‑1 (MQ=255)                                                           cATTAAACTTAATAATTTGTTCGATTTGTTTATCATCTTGTAATAAACTTTGCTCTTTAATCACATTTTTTAAAATGTCCCCATCAATATAATCAACGTg                               <  1:4803639/100‑1 (MQ=255)                                                            aTTAAACTTAATAATTTGTTCGATTTGTTTATCATCTTGTAATAAACTTTGCTCTTTAATCACATTTTTTAAAATGTCCCCATCAATATAATCAACGTGa                              <  1:5389566/100‑1 (MQ=255)                                                            aTTAAACTTAATAATTTGTGCGATTTGTTTATCATCTTGTAATAAACTTTGCTCTTTAATCACATTTTTTAAAATGTCCCCATCAATATAATCAACGTGa                              <  1:348865/100‑1 (MQ=255)                                                             ttAAACTTAATAATTTGTTCGATTTGTTTATCATCTTGTAATAAACTTTGCTCTTTAATCACATTTTTTAAAATGTCCCCATCAATATAATCAACGTgta                             <  1:5879460/100‑3 (MQ=255)                                                              tAAACTTAATAATTTGTTCGATTTGTTTATCATCTTGTAATAAACTTTGCTCTTTAATCACATTTTTTAAAATGTCCCCATCAATATAATCAACGTGAAt                            <  1:2808522/100‑1 (MQ=255)                                                              ctatcTTAATAATTTGTTCGATTTGTTTATCATCTTGTAATAAACTTTGCTCTTTAATCACATTTTTTAAAATGTCCCCATCAATATAATCAACGTGAAt                            <  1:4627280/96‑1 (MQ=255)                                                               aaaCTTAATAATTTGTTCGATTTGTTTATCATCTTGTAATAAACTTTGCTCTTTAATCACATTTTTTAAAATGTCCCCATCAATATAATCAACGTGAATa                           <  1:3733696/100‑1 (MQ=255)                                                               aaaCTTAATAATTTGTTCGATTTGTTTATCATCTTGTAATAAACTTTGCTCTTTAATCACATTTTTTAAAATGTCCCCATCAATATAATCAACGTGAATa                           <  1:1555829/100‑1 (MQ=255)                                                                                        ttATCATCTTGTAATAAACTTTGCTCTTTAATCACATTTTTTAAAATGTCCCCATCAATATAATCAACGTGAATAACAACAAAGCGATTTTTTAGTGCtt  <  1:2484731/100‑1 (MQ=255)                                                                                              |                                                                                              CGGATACTAGCGGCTTCTTCAGAAATTTGTCCCTGCTTAGACATAGTACGTAAATCTTCATTAAACTTAATAATTTGTTCGATTTGTTTATCACCTTGTAATAAACTTTGCTCTTTAATCACATTTTTTAAAATGTCCCCATCAATATAATCAACGTGAATAACAACAAAGCGATTTTTTAGTGCTT  >  NC_007793/1433434‑1433620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑