Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,423,567	G→A	A285T (GCT→ACT)	SAUSA300_RS07020 →	alanine racemase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,423,567	0	G	A	100.0%	33.9 / NA	14	A285T (GCT→ACT)	SAUSA300_RS07020	alanine racemase 2
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/1);  total (13/1)

TGCCTTTGAAGTGACTAAAAACAATACAAAATTAGCTGTAGTTGATATCGGTTATGGCGATGGAATTTTAAGAACTCGTGCTAAACATGAAGCACTTATCAATGGTAAACGCTACCCGATACGTGCATTAATGATGAGCCATATGTTTGTTGAAGTAGATGGCAATGTACAT  >  NC_007793/1423488‑1423659                                                                                |                                                                                             tGCCTCTGAAGTGACTAAAAACAATACAAAATTAGCTGTAGTTGATATCGGTTATGGCGATGGAATTTTAAGAACTCGTACTAAACATGAAGCACTTATc                                                                          >  1:5438455/1‑100 (MQ=255)           gTGACTAAAAACAATACAAAATTAGCTGTAGTTGATATCGGTTATGGCGATGGAATTTTAAGAACTCGTACTAAACATGAAGCACTTATCAATGGTAAAc                                                                >  1:3111627/1‑100 (MQ=255)                          aCAAAATTAGCTGTAGTTGATATCGGTTATGGCGATGGAATTTTAAGAACTCGTACTAAACATGAAGCACTTATCAATGGTAAACGCTACCCGATACGTg                                                 >  1:5108816/1‑100 (MQ=255)                                    cTGTAGTTGATATCGGTTATGGCGATGGAATTTTAAGAACTCGTACTAAACATGAAGCACTTATCAATGGTAAACGCTACCCGATACGTCCATTAatgat                                       >  1:4573692/1‑100 (MQ=255)                                           tGATATCGGTTATGGCGATGGAATTTTAAGAACTCGTACTAAACATGAAGCACTTATCAATGGTAAACGCTACCCGATACGTGCATTAATGATGAGCCat                                >  1:1175534/1‑100 (MQ=255)                                                    ttATGGCGATGGAATTTTAAGAACTCGTACTAAACATGAAGCACTTATCAATGGTAAACGCTACCCGATACGTGCATTAATGATGAGCCATAtgtttgtc                       >  1:1201946/1‑99 (MQ=255)                                                     tATGGCGATGGAATTTTAAGAACTCGTACTAAACATGAAGCACTTATCAATGGTAAACGCTACCCGATACGTGCATTAATGATGAGCCATATGTttgttg                      >  1:3308834/1‑100 (MQ=255)                                                     cctGGCGATGGAATTTTAAGAACTCGTACTAAACATGAAGCACTTATCAATGGTAAACGCTACCCGATACGTGCATTAATGATGAGCCATATGTttgttg                      >  1:5471377/3‑100 (MQ=255)                                                     caTGGCGATGGAATTTTAAGAACTCGTACTAAACATGAAGCACTTATCAATGGTAAACGCTACCCGATACGTGCATTAATGATGAGCCATAtgtttgttc                      >  1:3894845/2‑99 (MQ=255)                                                      aTGGCGATGGAATTTTAAGAACTCGTACTAAACATGAAGCACTTATCAATGGTAAACGCTACCCGATACGTGCATTAATGATGAGCCATATGTTTGTTGa                     >  1:1720720/1‑100 (MQ=255)                                                        ggCGATGGGATTTTAAGAACTCGTACTAAACATGAAGCACTTATCAATGGTAAACGCTACCCGATACGTGCATTAATGATGAGCCATATGTTTATTGAAg                   >  1:3080685/1‑100 (MQ=255)                                                         gCGATGAAATTTTAAGAACTCGTACTAAACATGAAGCACTTATCAATGGTAAACGCTACCCGATACGTGCATTAATGATGAGCCATATGTTTGTTGAAGt                  <  1:4839341/100‑1 (MQ=255)                                                          cGATGGAATTTTAAGAACTCGTACTAAACATGAAGCACTTATCAATGGTAAACGCTACCCGATACGTGCATTAATGATGAGCCATATGTTTGTTAAAGTa                 >  1:3180763/1‑100 (MQ=255)                                                                         gaCTCGTACTAAACATGAAGCACTTATCAATGGTAAACGCTACCCGATACGTGCATTAATGATGAGCCATATGTTTGTTGAAGTAGATGGCAATGTACAt  >  1:6504226/2‑100 (MQ=255)                                                                                |                                                                                             TGCCTTTGAAGTGACTAAAAACAATACAAAATTAGCTGTAGTTGATATCGGTTATGGCGATGGAATTTTAAGAACTCGTGCTAAACATGAAGCACTTATCAATGGTAAACGCTACCCGATACGTGCATTAATGATGAGCCATATGTTTGTTGAAGTAGATGGCAATGTACAT  >  NC_007793/1423488‑1423659

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑