Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	205,448	A→C	R578R (CGA→CGC)	SAUSA300_RS00950 →	non‑ribosomal peptide synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	205,448	0	A	C	100.0%	77.4 / NA	32	R578R (CGA→CGC)	SAUSA300_RS00950	non‑ribosomal peptide synthetase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (30/2);  total (30/2)

TGTAACACGTTAGAAGATCATGCTTATGTTATTTACACGTCGGGGACAACTGGTAACCCTAAAGGGACACTAATTCCGCACCGAGGTATTGTTCGATTGGTCCATCAAAATCATTATGTACCATTAAATGAAGAGACGACGATTTTGTTATCAGGAACTATAGCCTTTGATGCTGCAACATTTGAAATA  >  NC_007793/205353‑205541                                                                                                |                                                                                              tGTAACACGTTAGAAGATCATGCTTATGTTATTTACACGTCGGGGACAACTGGTAACCCTAAAGGGACACTAATTCCGCACCGAGGTATTGTTCGCTTgg                                                                                           >  1:1320886/1‑100 (MQ=255)    aaCACGTTAGAAGATCATGCTTATGTTATTTACACGTCGGGGACAACTGGTAACCCTAAAGGGACACTAATTCCGCACCGAGGTATTGTTCGCTTGGTcc                                                                                        >  1:5565045/1‑100 (MQ=255)    aaCACGTTAGAAGATCATGCTTATGTTATCTACACGTCGGGGACAACTGGTAACCCTAAAGGGACACTAATTCCGCACCGAGGTATTGTTCGCTTGGTcc                                                                                        >  1:5167185/1‑100 (MQ=255)              tagaTCATGCTTATGTTATTTACACGTCGGGGACAACTGGTAACCCTAAAGGGACACTAATTCCGCACCGAGGTATTGTTCGCTTGGTCCATCAAAATCa                                                                              >  1:1172673/2‑100 (MQ=255)                 aTCATGCTTATGTTATTTACACGTCGGGGACAACTGGTAACCCTAAAGGGACACTAATTCCGCACCGAGGTATTGTTCGCTTGGTCCATCAAAATCATTa                                                                           >  1:2500760/1‑100 (MQ=255)                  tCATGCTTATGTTATTTACACGTCGGGGACAACTGGTAACCCTAAAGGGACACTAATTCCGCACCGAGGTATTGTTCGCTTGGTCCATCAAAATCATTAt                                                                          >  1:1715521/1‑100 (MQ=255)                  tCATGCTTATGTTATTTACACGTCGGGGACAACTGGTAACCCTAAAGGGACACTAATTCCGCACCGAGGTATTGTTCGCTTGGTCCATCAAAATCATTAt                                                                          >  1:3019000/1‑100 (MQ=255)                   cATGCTTATGTTATTTACACGTCGGGGACAACTGGTAACCCTAAAGGGACACTAATTCCGCACCGAGGTATTGTTCGCTTGGTCCATCAAAATCATTATg                                                                         >  1:621600/1‑100 (MQ=255)                    aTGCTTATGTTATTTACACGTCGGGGACAACTGGTAACCCTAAAGGGACACTAATTCCGCACCGAGGTATTGTTCGCTTGGTCCATCAAAATCATTATGt                                                                        >  1:2544101/1‑100 (MQ=255)                         tATGTTATTTACACGTCGGGGACAACTGGTAACCCTAAAGGGACACTAATTCCGCACCGAGGTATTGTTCGCTTGGTCCATCAAAATCATTATGTACCag                                                                   >  1:6525741/1‑99 (MQ=255)                          aTGTTATTTACACGTCGGGGACAACTGGTAACCCTAAAGGGACACTAATTCCGCACCGAGGTATTGTTCGCTTGGTCCATCAAAATCATTATGTACCAtt                                                                  >  1:177147/1‑100 (MQ=255)                                 ttACACGTCGGGGACAACTGGTAACCCTAAAGGGACACTAATTCCGCACCGAGGTATTGTTCGCTTGGTCCATCAAAATCATTATGTACCATTAAATGaa                                                           >  1:1073277/1‑100 (MQ=255)                                  tACACGTCGGGGACAACTGGTAACCCTAAAGGGACACTAATTCCGCACCGAGGTATTGTTCGCTTGGTCCATCAAAATCATTATGTACCATTAAATGAag                                                          <  1:6389498/100‑1 (MQ=255)                                  tACACGTCGGGGACAACTGGTAACCCTAAAGGGACACTAATTCCGCACCGAGGTATTGTTCGCTTGGTCCATCAAAATCATTATGTACCATTAAATGAag                                                          >  1:1698035/1‑100 (MQ=255)                                  gacacGTCGGGGACAACTGGTAACCCTAAAGGGACACTAATTCCGCACCGAGGTATTGTTCGCTTGGTCCATCAAAATCATTATGTACCATTAAATGAag                                                          >  1:3858592/2‑100 (MQ=255)                                  cttccgatctggACAACTGGTAACCCTAAAGGGACACTAATTCCGCACCGAGGTATTGTTCGCTTGGTCCATCAAAATCATTATGTACCATTAAATCAag                                                          <  1:5934982/90‑1 (MQ=255)                                   acacGTCGGGGACAACTGGTAACCCTAAAGGGACACTAATTCCGCACCGAGGTATTGTTCGCTTGGTCCATCAAAATCATTATGTGCCATTAGAtcggaa                                                         >  1:5320222/1‑95 (MQ=255)                                   acacGTCGGGGACAACTGGTAACCCTAAAGGGACACTAATTCCGCACCGAGGTATTGTTCGCTTGGTCCATCAAAATCATTATGTACCAttgagatcgga                                                         >  1:2085485/1‑91 (MQ=255)                                   acacGTCGGGGACAACTGGTAACCCTAAAGGGACACTAATTCCGCACCGAGGTATTGTTCGCTTGGTCCATCAAAATCATTATGTACCATTAAATGAaga                                                         >  1:489226/1‑100 (MQ=255)                                   acacGTCGGGGACAACTGGTAACCCTAAAGGGACACTAATTCCGCACCGAGGTATTGTTCGCTTGGTCCATCAAAATCATTATGTACCATTAAATGAaga                                                         >  1:5887297/1‑100 (MQ=255)                                     tgcccGGGGACAACTGGTAACCCTAAAGGGACACTAATTCCGCACCGAGGTATTGTTCGCTTGGTCCATCAAAATCATTATGTACCATTAAATGAAgaga                                                       >  1:2382270/5‑100 (MQ=255)                                      cctCGGGGACAACTGGTAACCCTAAAGGGACACTAATTCCGCACCGAGGTATTGTTCGCTTGGTCCATCAAAATCATTATGTACCATTAAATGAAGAgac                                                      >  1:5785083/3‑100 (MQ=255)                                             gACAACTGGTAACCCTAAAGGGACACTAATTCCGCACCGAGGTATTGTTCGCTTGGTCCATCAGAATCATTATGTACCATTAagagaagagatcggaaga                                               >  1:5230381/1‑92 (MQ=255)                                             gACAACTGGTAACCCTAAAGGGACACTAATTCCGCACCGAGGTATTGTTCGCTTGGTCCATCAAAATCATTATGTACCATTAAATGAAgagatcggaaga                                               >  1:2973217/1‑92 (MQ=255)                                             gACAACTGGTAACCCTAAAGGGACACTAATTCCGCACCGAGGTATTGTTCGCTTGGTCCATCAAAATCATTATGTACCATTAAACAAAgagatcggaaga                                               >  1:6262855/1‑92 (MQ=255)                                               aaaCTGGGAACCCTAAAGGGGCACTAATTCCGCACCGAGGTATTGTTCGCTTGGGCCATCAAAATCATTATGTACAATTAAGTGATGAGACGACGAtttt                                             >  1:3611764/2‑100 (MQ=255)                                                 aCTGGTAACCCTAAAGGGACACTAATTCCGCACCGAGGTATTGTTCGCTTGGTCCATCAAAATCATTATGTACCATTAAATGAAGAGACGACGATTTTGt                                           >  1:273124/1‑100 (MQ=255)                                                       gtgccTAAAGGGACACTAATTCCGCACCGAGGTATTGTTCGCTTGGTCCATCAAAATCATTATGTACCATTAAATGAAGCGACGACGATTTTGTTATCAg                                     >  1:919968/4‑100 (MQ=255)                                                                            ccGCACCGAGGTATTGTTCGCTTGGTCCATCAAAATCATTATGTACCATTAAATGAAGAGACGACGCTTTTGTTATCAGGAACTATAGCCTTTGAtgctg                >  1:1444788/1‑100 (MQ=255)                                                                            ccGCACCGAGGTATTGTTCGCTTGGTCCATCAAAATCATTATGTACCATTAAATGAAGAGACGACGATTTTGTTATCAGGAACTATAGCCTTTGAtgctg                >  1:51568/1‑100 (MQ=255)                                                                                ctcGAGGTATTGTTCGCTTGGTCCATCAAAATCATTATGTACCATTAAATGAAGAGACGACGATTTTGTTATCAGGAACTATAGCCTTTGATGCTGCAAc            >  1:2031393/3‑100 (MQ=255)                                                                                          cactCGCTTGGTCCATCAAACTCATTCTGTACCATTAAATGTTGAGACGACGATTTTGTTATCAGGAACTATAGCCTTTGATGCTGCAACATTTGAAata  >  1:1097948/4‑100 (MQ=255)                                                                                                |                                                                                              TGTAACACGTTAGAAGATCATGCTTATGTTATTTACACGTCGGGGACAACTGGTAACCCTAAAGGGACACTAATTCCGCACCGAGGTATTGTTCGATTGGTCCATCAAAATCATTATGTACCATTAAATGAAGAGACGACGATTTTGTTATCAGGAACTATAGCCTTTGATGCTGCAACATTTGAAATA  >  NC_007793/205353‑205541

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑