Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,374,405	A→C	Y80S (TAC→TCC)	SAUSA300_RS06790 →	DNA topoisomerase 4 subunit A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,374,405	0	A	C	100.0%	149.6 / NA	57	Y80S (TAC→TCC)	SAUSA300_RS06790	DNA topoisomerase 4 subunit A
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (56/1);  total (56/1)

GCAATGTATTCAAGTGGTAATACACACGATAAAAATTTCCGTAAAAGTGCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTACTCAGTGTACGAAGCAATGGTCCGTTTAAGTCAAGACTGGAAGTTACGACATGTCTTAATAGAAATGCATGGTAATAATGGTAGTATCGATAAT  >  NC_007793/1374308‑1374499                                                                                                  |                                                                                               gCAATGTATTCAAGTGGTAATACACACGATAAAAATTTCCGTAAAAGTGCGACAACAGTCGGCGATGTTATTGGTCAATATCATCCACATGGAGactcct                                                                                              >  1:713606/1‑100 (MQ=255) gCAATGTATTCAAGTGGTAATACACACGATAAAAATTTCCGTAAAAGTGCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGCGactcct                                                                                              >  1:788767/1‑100 (MQ=255) gCAATGTATTCAAGTGGTAATACACACGATAAAAATTTCCGTAAAAGTGCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGactcct                                                                                              >  1:5162857/1‑100 (MQ=255) gCAATGTATTCAAGTGGTAATACACACGATAAAAATTTCCGTAAAAGTGCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGactcct                                                                                              >  1:6409053/1‑100 (MQ=255) gCAATGTATTCAAGTGGTAATACACACGATAAAAATTTCCGTAAAAGTGCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGactcct                                                                                              >  1:3633716/1‑100 (MQ=255) gCAATGTATTCAAGTGGTAATACACACGATAAAAATTTCCGTAAAAGTGCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGactcct                                                                                              >  1:5786573/1‑100 (MQ=255) gCAATGTATTCAAGTGGTAATACACACGATAAAAATTTCCGTAAAAGTGCGAAAACAGTCGGCGATGTTATTGGTCAATATCATCCACATGGAGactcct                                                                                              >  1:588224/1‑100 (MQ=255)   gctGTATTCAAGTGGTAATACACACGATAAAAATTTCCGTAAAAGTGCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCa                                                                                            >  1:88678/3‑100 (MQ=255)   gaTGTATTCAAGTGGTAATACACACGATAAAAATTTCCGTAAAAGTGCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCa                                                                                            >  1:4572858/2‑100 (MQ=255)   aaTGTATTCAAGTGGTAATACACACGATAAAAATTTCCGTAAAAGTGCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCa                                                                                            >  1:2928721/1‑100 (MQ=255)    aTGTATTCAAGTGGTAATACACACGATAAAAATTTCCGTAAAAGTGCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAg                                                                                           >  1:3841928/1‑100 (MQ=255)          tCAAGTGGTAATACACACGATAAAAATTTCCGTAAAAGTGCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTACg                                                                                     >  1:4181406/1‑100 (MQ=255)            aaGTGGTAATACACACGATAAAAATTTCCGTAAAAGTGCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTACGaa                                                                                   >  1:5618775/1‑100 (MQ=255)            aaGTGGTAATACACACGATAAAAATTTCCGTAAAAGTGCGAAAACAGTCGGTGATGTTATTGGACAATATCATCCACATGGAGACTCCTCAgatcggaag                                                                                   >  1:3312728/1‑92 (MQ=255)                ggTAATACACACGATAAAAATTTCCGTAAAAGTGCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTACGAAGCaa                                                                               >  1:1841558/1‑100 (MQ=255)                ggTAATACACACGATAAAAATTTCCGTAAAAGTGCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTACGAAGCaa                                                                               >  1:4678497/1‑100 (MQ=255)                   aaTACACACGATAAAAATTTCCGTAAAAGTGCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTACGAAGCAATgg                                                                            >  1:3909570/1‑100 (MQ=255)                   aaTACACACGATAAAAATTTCCGTAAAAGTGCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTACGAAGCAATgg                                                                            >  1:4165830/1‑100 (MQ=255)                      ccacacGATAAAAATTTCCGTAAAAGTGCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTACGAAGCAATGGTcc                                                                         >  1:5802552/2‑100 (MQ=255)                      acccacGATAAAAATTTCCGTAAAAGTGCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTACGAAGCAATGGTcc                                                                         >  1:937090/4‑100 (MQ=255)                      acacacGGTAAAAATTTCCGTAAAAGTGCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTACGAAGCAATGGTcc                                                                         >  1:5179409/1‑100 (MQ=255)                      acacacGATAAAAATTTCCGTAAAAGTGCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTACGAAGCAATGGTcc                                                                         >  1:1246388/1‑100 (MQ=255)                      acacacGATAAAAATTTCCGTAAAAGTGCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTACGAAGCAATGGTcc                                                                         >  1:4053347/1‑100 (MQ=255)                      acacacGATAAAAATTTCCGTAAAAGTGCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTACGAAGCAATGGTcc                                                                         >  1:3082309/1‑100 (MQ=255)                      acacacGATAAAAATTTCCGTAAAAGGACGTAAACAGTCGGTAATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTACGAAGCAATGGTca                                                                         >  1:6077608/1‑99 (MQ=255)                       cacacGATAAAAATTTCCGTAAAAGTGCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTACGAAGCAATGGTCCg                                                                        >  1:1064920/1‑100 (MQ=255)                        acacGATAAAAATTTCCGCAAAAGTGCGAAAACAGTCGGTGATGTTATTGCTCAATATCATCCACACGGAGACTCCTCAGTGTACGAAGCAATGGCCCGt                                                                       >  1:2172601/1‑100 (MQ=255)                          acGATAAAAATTTCCGTAAAAGTGCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTACGAAGCAATGGTCCGttt                                                                     >  1:227999/1‑100 (MQ=255)                                aaaaTTTCCGTAAAAGTGCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGTCa                                                               <  1:5912881/100‑1 (MQ=255)                                    aagccGTAAAAGTGCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGTCAAGAc                                                           >  1:1903645/4‑100 (MQ=255)                                     ttCCGTAAAAGTGCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGTCAAGACt                                                          >  1:5064615/1‑100 (MQ=255)                                      tCCGTAAAAGTGCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGTCAAGACTg                                                         >  1:4271948/1‑100 (MQ=255)                                       ccGTAAAAGTGCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTGAGTCgagatcgg                                                        >  1:4577184/1‑96 (MQ=255)                                       ccGTAAAAGTGCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTATGACAAGatcgg                                                        >  1:5811895/1‑96 (MQ=255)                                       ccGTAAAAGTGCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGTCAAGGCTgg                                                        >  1:5999612/1‑100 (MQ=255)                                       ccGTAAAAGTGCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGTCAAGACTgg                                                        >  1:227869/1‑100 (MQ=255)                                            ttaGTGCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGTCAAGACTGGAAGtt                                                   >  1:1551332/3‑100 (MQ=255)                                            taaGTGCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGCCAAGATCGGAAgag                                                   >  1:4383889/2‑98 (MQ=255)                                            aaaGTGCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGTCAAGACTGGAAGtt                                                   >  1:5897720/1‑100 (MQ=255)                                               gTGCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTACGAGGCAATGGTCCGTTTAAGTCAAGACTGGAAGTTACg                                                >  1:1445370/1‑100 (MQ=255)                                                 gCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGTCAAGACTGGAAGTTACGAc                                              >  1:4379102/1‑100 (MQ=255)                                                 gCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGTCAAGACTGGAAGTTACGAc                                              >  1:424342/1‑100 (MQ=255)                                                  cGAACACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGTCAAGACTGGAAgatcggaag                                             >  1:3020285/1‑92 (MQ=255)                                                                        tGGTCAATATCATCCACATGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGTCAAGACTGGAAGTTACGACATGTCTTAATAGAAATGCATgga                       >  1:4671829/1‑99 (MQ=255)                                                                         ggTCAATATCATTCACATGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGTCAAGACTGGAAGTTACGACATGTCTTAATAGAAATGCATGGta                      >  1:2494546/1‑100 (MQ=255)                                                                         ggTCAATATCATCCACATGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGTCAAGACTGGAAGTTACGACATGTCTTAATAGAAATGCATGGta                      >  1:5182734/1‑100 (MQ=255)                                                                            cAATATCATCCACATGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGTCAAGACTGGAAGTTACGACATGTCTTAATAGAAATGCATaataatg                   >  1:4001004/1‑99 (MQ=255)                                                                                  catcCACATGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGTCAGGACTGGAAGTTACGACATGTCTTAATAGAAATGCATGGTAATAATGgta             >  1:81264/1‑100 (MQ=255)                                                                                  catcCACATGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGTCAAGACTGGAAGTTACGACATGTCTTAATAGAAATGCATGGTAATAATGgta             >  1:1319916/1‑100 (MQ=255)                                                                                  catcCACATGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGTCAAGACTGGAAGATACGACATGTCTTAATAGAAATGCATGGTAATAATGgta             >  1:5928763/1‑100 (MQ=255)                                                                                    gtggcaTGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGTCAAGACTGGAAGTTACGACATGTCTTAATAGAAATGCATGGTAATAATGgtagt           >  1:6555579/5‑100 (MQ=255)                                                                                     ccACATGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGTCAAGACTGGGAGTTACGACATGTCTTAATAGAAATGCATGGTAATAATGgtagta          >  1:29875/1‑100 (MQ=255)                                                                                     ccACATGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGTCAAGACTGGAAGTTACGACATGTCTTAATAGAAATGCATGGTAATAATGgtagta          >  1:5253918/1‑100 (MQ=255)                                                                                     ccACATGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGTCAAGACTGGAAGTTACGACATGTCTTAATAGAAATGCATGGTAATAATGgtagta          >  1:3571517/1‑100 (MQ=255)                                                                                     ccACATGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGTCAAGACTGGAAGTTACGACATGTCTTAATAGAAATGCATGGTAATAATGgtagta          >  1:4031384/1‑100 (MQ=255)                                                                                     ccACATGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGTCAAGACTGGAAGTTACGACATGTCTTAATAGAAATGCATGGTAATAATGgtagta          >  1:6138664/1‑100 (MQ=255)                                                                                     ccACATGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGTCAAGACTGGAAGTTACGACATGTCTCAATAGAAATGCATGGTAATAATGgtagta          >  1:4406452/1‑100 (MQ=255)                                                                                     ccACATGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGTCAAGACTGGAAGTCACGACATGTCTTAATAGAAATGCATGGTAATAATGgtagta          >  1:5247498/1‑100 (MQ=255)                                                                                      cacaTGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGTCAAGACTGGAAGTTACGACATGTCTTAATAGAAATGCATGGTAATAATGGTAGTAt         >  1:265490/1‑100 (MQ=255)                                                                                       acaTGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGTCAAGACTGGAAGTTACGACATGTCTTAATAGAAATGCATGGTAATAATGGTAGTATc        >  1:5917223/1‑100 (MQ=255)                                                                                        caTGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGTCAAGACTGGAAGTTACGACATGTCTTAATAGAAATGCATGGTAATAATGGTAGTATCg       >  1:4062984/1‑100 (MQ=255)                                                                                         ctGGAGACTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGTCAAGACTGGAAGTTACGACATGTCTTAATAGAAATGCATGGTAATAATGGTAGTATCGa      >  1:6153262/2‑100 (MQ=255)                                                                                          ttgagaCTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGTCAAGACTGGAAGTTACGACATGTCTTAATAGAAATGCATGGTAATAATGGTAGTATCGAt     >  1:4374140/3‑100 (MQ=255)                                                                                             agaCTCCTCAGTGTACGAAGCAATGGTCCGTTTAAGTCAAGACTGGAAGTTACGACATGTCTTAATAGAAATGCATGGTAATAATGgtagatcggaagag  >  1:5883171/1‑90 (MQ=255)                                                                                                  |                                                                                               GCAATGTATTCAAGTGGTAATACACACGATAAAAATTTCCGTAAAAGTGCGAAAACAGTCGGTGATGTTATTGGTCAATATCATCCACATGGAGACTACTCAGTGTACGAAGCAATGGTCCGTTTAAGTCAAGACTGGAAGTTACGACATGTCTTAATAGAAATGCATGGTAATAATGGTAGTATCGATAAT  >  NC_007793/1374308‑1374499

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑