Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,363,207	A→T	I761F (ATT→TTT)	SAUSA300_RS06750 →	nuclease SbcCD subunit C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,363,207	0	A	T	100.0%	214.2 / NA	75	I761F (ATT→TTT)	SAUSA300_RS06750	nuclease SbcCD subunit C
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (75/0);  total (75/0)

AACTGATGACAATGATATAGATGAAATAATAGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAAATTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGATAAATTAAAAGATGAATATGAGCTAAAAAAAGGAA  >  NC_007793/1363110‑1363303                                                                                                  |                                                                                                 aaCTGATGACAATGATATAGATGAAATAATAGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCAtgaattt                                                                                                >  1:3701500/1‑100 (MQ=255)     gatgaCAATGATATAGATGAAATAATAGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCAtgaatttgaaa                                                                                            >  1:4434756/1‑100 (MQ=255)     gatgaCAATGATATAGATGAAATAATAGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCAtgaatttgaaa                                                                                            >  1:4721314/1‑100 (MQ=255)        gaCAATGATATAGATGAAATAATAGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATgg                                                                                         >  1:3991367/1‑100 (MQ=255)          cAATGATATAGATGAAATAATAGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGaa                                                                                       >  1:4363069/1‑100 (MQ=255)            ctGATATAGATGAAATAATAGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCAtgaatttgaagatcggaa                                                                                     >  1:5433875/2‑92 (MQ=255)                 ataGATGAAATAATAGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTa                                                                                >  1:4189380/1‑100 (MQ=255)                   aGATGAACTAATAGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAgg                                                                              >  1:1978919/1‑100 (MQ=255)                    gATGAAATAATAGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGt                                                                             >  1:1095035/1‑100 (MQ=255)                            tataGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCa                                                                     >  1:5949098/2‑100 (MQ=255)                            tataGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTCAGatcgg                                                                     >  1:1479209/2‑96 (MQ=255)                            cataGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCa                                                                     >  1:4759307/2‑100 (MQ=255)                            cataGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCa                                                                     >  1:1061784/2‑100 (MQ=255)                            aataGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCa                                                                     >  1:1342330/1‑100 (MQ=255)                            aataGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCa                                                                     >  1:1543545/1‑100 (MQ=255)                             ctaGCCTGGAGGGGCGAGCAAGAGAAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCAt                                                                    >  1:3095652/2‑100 (MQ=255)                             ataGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGCAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCAt                                                                    >  1:4594129/1‑100 (MQ=255)                             ataGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCAt                                                                    >  1:3364096/1‑100 (MQ=255)                             ataGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCAt                                                                    >  1:5562436/1‑100 (MQ=255)                             ataGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCAt                                                                    >  1:1356591/1‑100 (MQ=255)                             ataGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCAt                                                                    >  1:5229674/1‑100 (MQ=255)                              taGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCAtt                                                                   >  1:1213125/1‑100 (MQ=255)                              taGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCAtt                                                                   >  1:1129868/1‑100 (MQ=255)                               aGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTa                                                                  >  1:5960847/1‑100 (MQ=255)                               aGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTa                                                                  >  1:2977281/1‑100 (MQ=255)                               aGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTa                                                                  >  1:3707938/1‑100 (MQ=255)                                  cacagggggCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACt                                                               >  1:5986388/6‑100 (MQ=255)                                   tGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTa                                                              >  1:3642855/1‑100 (MQ=255)                                   atttagggCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTa                                                              >  1:1309090/6‑100 (MQ=255)                                     taGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAg                                                            >  1:1327409/2‑100 (MQ=255)                                     aaGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAg                                                            >  1:1251653/2‑100 (MQ=255)                                       agtgtgcgCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGa                                                          >  1:2031872/8‑100 (MQ=255)                                         agCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATa                                                        >  1:3426060/2‑100 (MQ=255)                                          gCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATaa                                                       >  1:1439731/1‑100 (MQ=255)                                           cGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATaaa                                                      >  1:6075520/1‑100 (MQ=255)                                             aGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAaga                                                    >  1:4527638/1‑100 (MQ=255)                                              gCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATTATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAagag                                                   >  1:4097101/1‑100 (MQ=255)                                              gCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAagag                                                   >  1:4661482/1‑100 (MQ=255)                                                cagagGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGtt                                                 >  1:1485504/2‑100 (MQ=255)                                                 agagGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTa                                                >  1:1221433/1‑100 (MQ=255)                                                 agagGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTa                                                >  1:3209120/1‑100 (MQ=255)                                                          ctcacggAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAGTCATTAACTAAGGATAAAGAGTTATTGGActct                                       >  1:3914876/7‑100 (MQ=255)                                                          actccAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGActct                                       >  1:4704138/5‑100 (MQ=255)                                                           acgCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTg                                      >  1:975229/3‑100 (MQ=255)                                                           aagCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTg                                      >  1:2867657/2‑100 (MQ=255)                                                            ggCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGa                                     >  1:6102844/2‑100 (MQ=255)                                                            agCGGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGa                                     >  1:5377899/1‑100 (MQ=255)                                                            agCAGAAAAGGGATACTTATAAAAAACGTTGTCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGa                                     >  1:1103228/1‑100 (MQ=255)                                                            agCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGa                                     >  1:4208847/1‑100 (MQ=255)                                                             tcggAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGAt                                    >  1:6420046/4‑100 (MQ=255)                                                             gCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGAt                                    >  1:2956724/1‑100 (MQ=255)                                                             gCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGAt                                    >  1:3295389/1‑100 (MQ=255)                                                             gCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGAt                                    >  1:3414312/1‑100 (MQ=255)                                                             gCAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGAt                                    >  1:1326791/1‑100 (MQ=255)                                                             ccAGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGAt                                    >  1:2930442/2‑100 (MQ=255)                                                               aGAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGATaa                                  >  1:1076261/1‑100 (MQ=255)                                                                gAAAAGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGATaaa                                 >  1:4808512/1‑100 (MQ=255)                                                                   taGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGATAAATTa                              >  1:1157214/2‑100 (MQ=255)                                                                   caGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGATAAATTa                              >  1:5009158/2‑100 (MQ=255)                                                                   aaGGGATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGATAAAtag                              >  1:4757629/1‑98 (MQ=255)                                                                      ggATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGATAAATTaaaa                           >  1:4516749/1‑100 (MQ=255)                                                                       gATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGATAAATTAAAAg                          >  1:468351/1‑100 (MQ=255)                                                                       gATACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGATAAATTAAAAg                          >  1:4784078/1‑100 (MQ=255)                                                                       cctACTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGATAAATTAAAAg                          >  1:3994992/3‑100 (MQ=255)                                                                          aCTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGATAAATTAAAAGAta                       >  1:6564574/1‑99 (MQ=255)                                                                          aCTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGATAAATTAAAAGATg                       >  1:4185108/1‑100 (MQ=255)                                                                          aCTTATAAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGCACTCTGATAAATTAAAAGATg                       >  1:4767172/1‑100 (MQ=255)                                                                             tataAAAAACGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGATAAATTAAAAGATGAat                    >  1:5366677/1‑100 (MQ=255)                                                                                aaaaaaCGTTATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGATAAATTAAAAGATGAATATg                 >  1:4018507/1‑100 (MQ=255)                                                                                        ttATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGATAAATTAAAAGATGAATATGAGCTaaaa         >  1:5613630/1‑100 (MQ=255)                                                                                        ttATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGATAAATTAAAAGATGAATATGAGCTaaaa         >  1:6627360/1‑100 (MQ=255)                                                                                         tATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGGGTTATTGGACTCTGATAAATTAAAAGATGAATATGAGCTaaaaa        >  1:2238571/1‑100 (MQ=255)                                                                                         tATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGATACATTAAAAGATGAATATGAGCTaaaaa        >  1:2579769/1‑100 (MQ=255)                                                                                         tATCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGATAAATTAAAAGATGAATATGAGCTaaaaa        >  1:1844426/1‑100 (MQ=255)                                                                                          aTCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGATAAATTAAAAGATGAATATGAGCTaaaaga       >  1:3612267/1‑98 (MQ=255)                                                                                          aTCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGATAAATTAAAAGATGAATATGAGCTaaaaaa       >  1:1203807/1‑100 (MQ=255)                                                                                          aTCATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGATAAATTAAAAGATGAATATGAGCTaaaaaa       >  1:3716552/1‑100 (MQ=255)                                                                                            cATGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGTGATATTGGACTCTGATAAATTAAAAGATGAATATGAGCTaaaaaaat     >  1:1021786/1‑99 (MQ=255)                                                                                             aTGAATTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGATAAATTAAAAGATGAATATGAGCTaaaaaagag    >  1:4491350/1‑97 (MQ=255)                                                                                               gaatttgaaatGGAAATAGCTAGGTTAGAATCATTATCTAAGGATAAAGAGTTATTGGACTCTGATAAATTAAAAGATGAATATGAGCTAAAAAAAGGaa  <  1:5221927/100‑1 (MQ=255)                                                                                               gaatttgaaatGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGATAAATTAAAAGATGAATATGAGCTAAAAAAAGGag  <  1:237252/100‑2 (MQ=255)                                                                                               gaatttgaaatGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGATAAATTAAAAGATGAATATGAGCTAAAAAAAGGaa  >  1:1958517/1‑100 (MQ=255)                                                                                                  |                                                                                                 AACTGATGACAATGATATAGATGAAATAATAGCCTGGAGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAAATTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCTGATAAATTAAAAGATGAATATGAGCTAAAAAAAGGAA  >  NC_007793/1363110‑1363303

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑