D3A :: BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,357,593	C→A	A357D (GCT→GAT)	SAUSA300_RS06725 →	transketolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,357,593	0	C	A	99.3%	411.6 / NA	143	A357D (GCT→GAT)	SAUSA300_RS06725	transketolase
Reads supporting (aligned to +/- strand): ref base C (1/0); major base A (138/3); minor base T (1/0); total (140/3)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 144 total aligned reads displayed.

AGAAGAATTTAAATTAGCGATTAGTGGTAAATTGCCTAAAAATTATAAGGATGAATTACCACGTTTTGAACTGGGTCATAATGGTGCATCTCGTGCTGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATCAGCAGACCTTGCTGGTTCAAACAAATCCAATGTAAA  >  NC_007793/1357498‑1357689

tgaagaaTTTAAATTAGCGATTAGTGGTAAATTGCCTAAAAATTATAAGGATGAATTACCACGTTTTGAACTGGGTCATAATGGTGCATCTCGTgatgat                                                                                              >  1:927616/2‑100 (MQ=255)

   aaggggtAAATTAACGATTAGTGGTAAATTGCCTAAAAATTATAAGGATGAATTACCACGTTTTGAACTGGGTCATAATGGTGCATCTCGTGATGATTCt                                                                                           >  1:6109091/7‑100 (MQ=255)

      aTTTAAATTAGCGATTAGTGGTAAATTGCCTAAAAATTATAAGGATGAATTACCACGTTTTGAACTGGGTCATAATGGTGCGTCTCGTGATGATTCTGGt                                                                                        >  1:90123/1‑100 (MQ=255)

          aaaTTAGCGATTAGTGGTAAATTGCCTAAAAATTATAAGGATGAATTACCACGTTTTGAACTGGGTCATAATGGTGCATCTCGTGATGATTCTGGTACTg                                                                                    >  1:2583600/1‑100 (MQ=255)

          aaaTTAGCGATTAGTGGTAAATTGCCTAAAAATTATAAGGATGAATTACCACGTTTTGAACTGGGTCATAATGGTGCATCTCGTGATGATTCTGGTACTg                                                                                    >  1:5032617/1‑100 (MQ=255)

              tAGCGATTAGTGGTAAATTGCCTAAAAATTATAAGGATGAATTACCACGTTTTGAACTGGGTCATAATGGTGCATCTCGTGATGATTCTGGTACTGTTAt                                                                                >  1:4893352/1‑100 (MQ=255)

              tAGCGATTAGTGGTAAATTGCCTAAAAATTATAAGGATGAATTACCACGTTTTGAACTGGGTCATAATGGTGCATCTCGTGATGATTCTGGTACTGTTAt                                                                                >  1:4898314/1‑100 (MQ=255)

              aaGCGATTAGCGGCAAATTGCCTAAAAATTATAAGGATGAATTACCACGTTTTGAACTGGGCCATAATGGTGCATCTCGTGATGATTCTGGTACTGTTAt                                                                                >  1:6352643/2‑100 (MQ=255)

               aGCGATTAGTGGTAAATTGCCTAAAAATTATAAGGATGAATTACCACGTTTTGAACTGGGTCATAATGGTGCATCTCGTGATGATTCTGGTACTGTTAtt                                                                               >  1:5345618/1‑100 (MQ=255)

               aGCGATTAGTGGTAAATTGCCTAAAAATTATAAGGATGAATTACCACGTTTTGAACTGGGTCATAATGGTGCATCTCGTGATGATTCTGGTACTGTCAtt                                                                               >  1:3514447/1‑100 (MQ=255)

                 cGATTAGTGGTAAATTGCCTAAAAATTATAAGGATGAATTACCACGTTTTGAACTGGGTCATAATGGTGCATCTCGTGATGATTCTGGTACTGTTATTCa                                                                             <  1:921088/100‑1 (MQ=255)

                            aaaTTGCCTAAAAATTATAAGGATGAATTACCACGTTTTGAACTGGGTCATAATGGTGCATCTCGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTa                                                                  >  1:1482631/1‑100 (MQ=255)

                             aaTTGCCTAAAAATTATAAGGATGAATTACCACGTTTTGAACTGGGTCATAATGGTGCATCTCGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTaa                                                                 >  1:252586/1‑100 (MQ=255)

                             aaTTGCCTAAAAATTATAAGGATGAATTACCACGCTTTGAACTGGGTCATAATGGTGCATCTCGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTaa                                                                 >  1:1500602/1‑100 (MQ=255)

                              cctGCCTAAAAATTATAAGGATGAATTACCACGTTTTGAACTGGGTCATAATGGTGCATCTCGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTaaa                                                                >  1:2090064/3‑100 (MQ=255)

                              aTTGCCTAAAAATTATAAGGATGAATTACCACGTTTTGGACTGGGTCATAATGGTGCATCTCGTGATGATTCTGGTACTGTTATTCAAGCAATCAgatcg                                                                >  1:1794513/1‑96 (MQ=255)

                                tGCCTAAAAATTATAAGGATGAATTACCACGTTTTGAACTGGGTCATAATGGTGCATCTCGTGATGATTCTGGTACTGTTATTCAAGCAATCAgatcgga                                                              >  1:6386834/1‑94 (MQ=255)

                                tGCCTAAAAATTATAAGGATGAATTACCACGTTTTGAACTGGGTCATAATGGTGCATCTCGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAAc                                                              >  1:1244199/1‑100 (MQ=255)

                                 gCCTAAAAATTATAAGGATGAATTACCACGTTTTGAACTGGGTCATAATGGTGCATCTCGTGATGATTCTGGTACTGTTATTCAATCAATCAGTAAAACt                                                             >  1:3295855/1‑100 (MQ=255)

                                  ccTAAAAATTATAAGGATGAATTACCACGTTTTGAACTGGGTCATAATGGTGCATCTCGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTg                                                            >  1:3969346/1‑100 (MQ=255)

                                          acataAGGATGAATTACCACGTTTTGAACTGGGTCATAATGGTGCATCTCGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCa                                                    >  1:5964391/3‑100 (MQ=255)

                                            ataAGGATGAATTACCACGTTTTGAACTGGGTCATAATGGTGCATCTCGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCAtt                                                  >  1:333227/1‑100 (MQ=255)

                                              aaGGATGAATTACCACGTTTTGAACTGGGTCATAATGGTGCATCTCGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCt                                                >  1:3151240/1‑100 (MQ=255)

                                                    gAATTACCACGTTTTGAACTGGGTCATAATGGTGCATCTCGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTtggtg                                          >  1:4005753/1‑100 (MQ=255)

                                                        tACCACGTTTTGAACTGGGTCATAATGGTGCATCTCGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATc                                      >  1:5126682/1‑100 (MQ=255)

                                                        tACCACGTTTTGAACTGGGTCATAATGGTGCATCTCGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATc                                      >  1:2322130/1‑100 (MQ=255)

                                                        tACCACGTTTTGAACTGGGTCATAATGGTGCATCTCGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATc                                      >  1:5866115/1‑100 (MQ=255)

                                                        tACCACGTTTTGAACTGGGTCATAATGGTGCATCTCGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTAGGTAGATc                                      >  1:4151746/1‑100 (MQ=255)

                                                        tACCACGTTTTGAACTGGGTCATAATGGTGCATCTCGTGATGATTCTGGTACTGTTATCCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATc                                      >  1:2693050/1‑100 (MQ=255)

                                                        tACCACGTTTTGAACTGGGTCATAATGGAGCACCTCGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGGTc                                      >  1:215426/1‑100 (MQ=255)

                                                         gccACGTTTTGAACTGGGTCATAATGGTGCATCTCGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATca                                     >  1:246103/2‑100 (MQ=255)

                                                         aCCACGTTTTGAACTGGGTCATAATGGTGCATCTCGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATca                                     >  1:2409662/1‑100 (MQ=255)

                                                         aCCACGTTTTGAACTGGGTCATAATGGTGCATCTCGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATca                                     >  1:264278/1‑100 (MQ=255)

                                                          acACGTTTTGAACTGGGTCATAATGGTGCATCTCGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATcag                                    >  1:141588/2‑100 (MQ=255)

                                                                cttGAACTGGGTCATAATGGTGCATCTCGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATCAGCAGAcc                              >  1:2857240/2‑100 (MQ=255)

                                                                 ttGAACTGGGTCATAATGGTGCATCTCGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATCAGCAGACCt                             >  1:1826394/1‑100 (MQ=255)

                                                                  tGAACTGGGTCATAATGGTGCATCTCGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATCAGCAGACCtt                            >  1:2751257/1‑100 (MQ=255)

                                                                    taCTGGGTCATAATGGTGCATCTCGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTAGATCGGAAGagcacac                          >  1:2125819/2‑94 (MQ=255)

                                                                    cgcTGGGTCATAATGGTGCATCTCGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATCAGCAGACCTTGc                          >  1:1337954/3‑100 (MQ=255)

                                                                    aaCTGGGTCATAATGGTGCATCTCGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATCAGCAGACCTTGc                          >  1:3282851/1‑100 (MQ=255)

                                                                    aaCTGGGTCATAATGGTGCATCTCGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATCAGCAGACCTTGc                          >  1:2196826/1‑100 (MQ=255)

                                                                     gcTGGGTCATAATGGTGCATCTCGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATCAGCAGACCTTGCt                         >  1:3091055/2‑100 (MQ=255)

                                                                     ccTGGGTCATAATGGTGCATCTCGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATCAGCAGACCTTGCt                         >  1:6111992/2‑100 (MQ=255)

                                                                     aCTGGGTCATAATGGTGCATCTCGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATCAGCAGACCTTGCt                         >  1:2968465/1‑100 (MQ=255)

                                                                      cTGGGTCATAATGGTGCATCTCGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATCAGCAGACCTTGCTg                        >  1:4026866/1‑100 (MQ=255)

                                                                      cTGGGTCATAATGGTGCATCTCGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATCAGCAGACCTTGCTg                        >  1:1730672/1‑100 (MQ=255)

                                                                       cgggTCATAATGGTGCATCTCGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATCAGCAGACCTTGCTgg                       >  1:4560560/2‑100 (MQ=255)

                                                                        gggTCATAATGGTGCATCTCGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATCAGCAGACCTTGCTGGt                      >  1:4609255/1‑100 (MQ=255)

                                                                        gggTCATAATGGTGCATCTCGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATCAGCAGACCTTGCTGGt                      >  1:1090112/1‑100 (MQ=255)

                                                                        gggTCATAATGGTGCATCTCGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATCAGCAGACCTTGCTGGt                      >  1:1176836/1‑100 (MQ=255)

                                                                        gggTCATAATGGTGCATCTCGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATCAGCAGACCTTGCTGGt                      >  1:3078224/1‑100 (MQ=255)

                                                                        gggTCATAATGGTGCATCTCGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATCAGCAGACCTTGCTGGt                      >  1:1596165/1‑100 (MQ=255)

                                                                        gggTCATAATGGTGCATCTCGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATCAGCAGACCTTGCTGGt                      >  1:2882856/1‑100 (MQ=255)

                                                                         gcgcATAATGGTGCATCTCGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAGACTGTCCCTTCATTCTTTGGTGTATCAGCAGACCTTGCTGGtt                     >  1:1460608/4‑100 (MQ=255)

                                                                           ccATAATGGTGCATCTCGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATCAGCAGACCTTGCTGGTTca                   >  1:599840/2‑100 (MQ=255)

                                                                            cATAATGGTGCATCTCGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATCAGCAGACCTTGCTGGTTcaa                  >  1:5787986/1‑100 (MQ=255)

                                                                             tacctGGTGCATCTCGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATCAGCAGACCTTGCTGGTTcaaa                 >  1:3801310/5‑100 (MQ=255)

                                                                               aaTGGTGCATCTCGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATCAGCAGACCTTGCTGGTTcaaaca               >  1:1894666/1‑100 (MQ=255)

                                                                                ttGGTGCATCTCGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATCAGCAGACCTTGCTGGTTcaaacaa              >  1:4233644/2‑100 (MQ=255)

                                                                                aTGGCGCATCTCGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATCAGCAGACCTTGCTGGTTcaaacaa              >  1:5339977/1‑100 (MQ=255)

                                                                                   tggCATCTCGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATCAGCAGACCTTGCTGGTTCAAACAAATc           >  1:5653873/3‑100 (MQ=255)

                                                                                     gCATCTCGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATCAGCAGACCTTGCTGGTTCAAACAAATCCa         >  1:5868275/1‑100 (MQ=255)

                                                                                         ctcGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATCAGCAGACCTTGCTGGTTCAAACAAATCCAATGt     >  1:3417915/1‑100 (MQ=255)

                                                                                         ctcGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATCAGCAGACCTTGCTAGTTCAAACAAATCCAATGt     >  1:770906/1‑100 (MQ=255)

                                                                                           cGTGTTGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATCAGCAGACCTTGCTGGTTCAAACAAATCCAATGTaa   >  1:6280351/1‑100 (MQ=255)

                                                                                           cGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATCAGCAGACCTTGCTGGTTCAACCAAATCCAATGTaa   >  1:5000902/1‑100 (MQ=255)

                                                                                           cGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATCAGCAGACCTTGCTGGTTCAACCAAATCCAATGTaa   >  1:4789909/1‑100 (MQ=255)

                                                                                           cGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATCAGCAGACCTTGCTGGTTCAAACAAATCCAATGTaa   >  1:2256151/1‑100 (MQ=255)

                                                                                           cGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATCAGCAGACCTTGCTGGTTCAAACAAATCCAATGTaa   >  1:5552179/1‑100 (MQ=255)

                                                                                           cGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATCAGCAGACCTTGCTGGTTCAAACAAATCCAATGTaa   >  1:1632670/1‑100 (MQ=255)

                                                                                           cGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATCAGCAGACCTTGCTGGTTCAAACAAATCCAATGTaa   >  1:1769149/1‑100 (MQ=255)

                                                                                           cGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATCAGCAGACCTTGCTGGTTCAAACAAATCCAATGTaa   >  1:2524483/1‑100 (MQ=255)

                                                                                           cGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATCAGCAGACCTTGCTGGTTCAAACAAATCCAATGTaa   >  1:6256126/1‑100 (MQ=255)

                                                                                           cGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATCAGCAGACCTTGCTGGTTCAAACAAATCCAATGTaa   >  1:6316972/1‑100 (MQ=255)

                                                                                           cGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATCAGCAGACCTTGCTGGTTCAAACAAATCCAATGTaa   >  1:641798/1‑100 (MQ=255)

                                                                                           cGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATCAGCAGACCTTGCTGGTTCAAACAAATCCAATGTaa   >  1:761050/1‑100 (MQ=255)

                                                                                           cGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATCAGCAGACCTTGCTGGTTCAAACAAATCCAATGTaa   >  1:1128692/1‑100 (MQ=255)

                                                                                           cGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATCAGCAGACCTTGCTGGTTCAAACAAATCCAATGTaa   >  1:1014805/1‑100 (MQ=255)

                                                                                           cGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATCAGCAGACCTTGCTGGTTCAAACAAATCCAATGTaa   >  1:4239220/1‑100 (MQ=255)

                                                                                           cGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATCAGCAGACCTTGCTGGTTCAAACAAATCCAATGTaa   >  1:3575042/1‑100 (MQ=255)

                                                                                           cGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATCAGCAGACCTTGCTGGTTCAAACAAATCCAATGTaa   >  1:3651246/1‑100 (MQ=255)

                                                                                           cGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATCAGCAGACCTTGCTGGTTCAAACAAATCCAATGTaa   >  1:3921499/1‑100 (MQ=255)

                                                                                           cGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATCAGCAGACCTTGCTGGTTCAAACAAATCCAATGTaa   >  1:3959160/1‑100 (MQ=255)

                                                                                           cGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATCAGCAGACCTTGCTGGTTCAAACAAATCCAATGTaa   >  1:3413590/1‑100 (MQ=255)

                                                                                           cGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATCAGCAGACCTTGCTGGTTCAAACAAATCCAATGTaa   >  1:3991905/1‑100 (MQ=255)

                                                                                           cGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATCAGCAGACCTTGCTGGTTCAAACAAATCCAATGTaa   >  1:3401383/1‑100 (MQ=255)

                                                                                           cGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATCAGCAGACCTTGCTGGTTCAAACAAATCCAATGTaa   >  1:3259852/1‑100 (MQ=255)

                                                                                           cGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATCAGCAGACCTTGCTGGTTCAAACAAATCCAATGTaa   >  1:412814/1‑100 (MQ=255)

                                                                                           cGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATCAGCAGACCTTGCTGGTTCAAACAAATCCAATGTaa   >  1:5408458/1‑100 (MQ=255)

                                                                                           cGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATCAGCAGACCTTGCTGGTTCAAACAAATCCAATGTaa   >  1:4526289/1‑100 (MQ=255)

                                                                                           cGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATCAGCAGACCTTGCTGGTTCAAACAAATCCAATGTaa   >  1:4587346/1‑100 (MQ=255)

                                                                                           cGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATCAGCAGACCTTGCTGGTTCAAACAAATCCAATGTaa   >  1:3026719/1‑100 (MQ=255)

                                                                                           cGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATCAGCAGACCTTGCTGGTTCAAACAAATCCAATGTaa   >  1:2981945/1‑100 (MQ=255)

                                                                                           cGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATCAGCAGACCTTGCTGGTTCAAACAAATCCAATGTaa   >  1:2540588/1‑100 (MQ=255)

                                                                                           cGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATCAGCAGACCTTGCTGGTTCAAACAAATCCAATGTaa   >  1:5106582/1‑100 (MQ=255)

                                                                                           cGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATCAGCAGACCTTGCTGGTTCAAACAAATCCAATGTaa   >  1:2363325/1‑100 (MQ=255)

                                                                                           cGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATCAGCAGACCTTGCTGGTTCAAACAAATCCAATGTaa   >  1:5351257/1‑100 (MQ=255)

                                                                                           cGTGATGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATCAGCAGACCCTGCTGGTTCAAACAAATCCAATGTaa   >  1:5684815/1‑100 (MQ=255)

                                                                                           cGTGATGATTCTGGTACTGCTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATCAGCAGACCTTGCTGGTTCAAAC‑AATCCAATGTaaa  >  1:3240075/1‑100 (MQ=255)

                                                                                           cGTGATGATCCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATCAGCAGACCTTGCTGGTTCAAACAAATCCAATGTaa   >  1:3573254/1‑100 (MQ=255)

AGAAGAATTTAAATTAGCGATTAGTGGTAAATTGCCTAAAAATTATAAGGATGAATTACCACGTTTTGAACTGGGTCATAATGGTGCATCTCGTGCTGATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATCAGCAGACCTTGCTGGTTCAAACAAATCCAATGTAAA  >  NC_007793/1357498‑1357689

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`