Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,333,945	C→T	S6L (TCA→TTA)	SAUSA300_RS06595 →	cardiolipin synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,333,945	0	C	T	100.0%	74.1 / NA	28	S6L (TCA→TTA)	SAUSA300_RS06595	cardiolipin synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/0);  total (28/0)

GTGAATAAATAATATGACGTGAAAGGTGCAAAATGTTTTTTTGTGCCTTTCTTTATAGGAGAAAAGACTATGCGATTTACATTTTCAAACGATTTAGGAACGTTATTTACTATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGAA  >  NC_007793/1333860‑1334037                                                                                      |                                                                                             gtgAATAAATAATATGACGTGAAAGGTGCAAAATGTTTTTTTGTGCCTTTCTTTATAGGAGAAAAGACTATGCGATTTACATTTTTAAACGATTTAGGaa                                                                                >  1:1572771/1‑100 (MQ=255) gtgAATAAATAATATGACGTGAAAGGTGCAAAATGTTTTTTTGTGCCTTTCTTTATAGGAGAAAAGACTATGCGATTTACATTTTTAAACGATTTAGGaa                                                                                >  1:5600494/1‑100 (MQ=255)           aataTGACTTGAAAGGTGCAAAATGTTTTTTTGTGCCTTTCTTTATAGGAGAAAAGACTATGCGATTTACATTTTTAAACGATTTAGGAACGTTATTTAc                                                                      >  1:6259000/1‑100 (MQ=255)              ctgccGTGAAAGGTGCAAAATGTTTTTTTGTGCCTTTCTTTATAGGAGAAAAGACTATGCGATTTACATTTTTAAACGATTTAGGAACGTTATTTACtat                                                                   >  1:6018003/5‑100 (MQ=255)                      aaaGGTGCAAAATGTTTTTTTGTGCCTTTCTTTATAGGAGAAAAGACTATGCGATTTACATTTTTAAACGATTTAGGAACGTTATTTACTATTATTTTAg                                                           >  1:3170711/1‑100 (MQ=255)                                                     ttATAGGAGAAAAGACTATGCGATTTACATTTTTAAACGATTTAGGAACGTTATTTACTATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGc                            >  1:1552279/1‑100 (MQ=255)                                                     ttATAGGAGAAAAGACTATGCGATTTACATTTTTAAACGATTTAGGAACGTTATTTACTATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGc                            >  1:2098408/1‑100 (MQ=255)                                                      cctaGGAGAAAAGACTATGCGATTTACATTTTTAAACGATTTAGGAACGTTATTTACTATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCt                           >  1:1793871/3‑100 (MQ=255)                                                       ataGGAGAAAAGACTATGCGATTTACATTTTTAAACGATTTAGGAACGTTATTTACTATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCtt                          >  1:1639651/1‑100 (MQ=255)                                                               aaaGACTATGCGATTTACATTTTTAAACGATTTAGGAACGTTATTTACTATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTttattatt                  >  1:1689196/1‑100 (MQ=255)                                                               aaaGACTATGCGATTTACATTTTTAAACGATTTAGGAACGTTATTTACTATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTttattatt                  >  1:5538370/1‑100 (MQ=255)                                                                   aCTATGCGATTTACATTTTTAAACGATTTAGGAACGTTATTTACTATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCt              >  1:4710982/1‑100 (MQ=255)                                                                    cTATGCGATTTACATTTTTAAACGATTTAGGAACGTTATTTACTATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCtt             >  1:5222431/1‑100 (MQ=255)                                                                     catccGATTTACATTTTTAAACGATTTAGGAACGTTATTTACTATTATTTTATCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCttt            >  1:263272/5‑100 (MQ=255)                                                                      aTGCGATTTACATTTTTAAACGATTTGGGAACGTTATTTACTATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCtttt           >  1:2775729/1‑100 (MQ=255)                                                                      aTGCGATTTACATTTTTAAACGATTTAGGAACGTTATTTACTATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTTTTATCtttt           >  1:6432962/1‑100 (MQ=255)                                                                      aTGCGATTTACATTTTTAAACGATTTAGGAACGTTATTTACTATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCtttt           >  1:6324345/1‑100 (MQ=255)                                                                      aTGCGATTTACATTTTTAAACGATTTAGGAACGTTATTTACTATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCtttt           >  1:409881/1‑100 (MQ=255)                                                                      aTGCGATTTACATTTTTAAACGATTTAGGAACGTTATTTACTATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCtttt           >  1:4537403/1‑100 (MQ=255)                                                                      aTGCGATTTACATTTTTAAACGATTTAGGAACGTTATTTACTATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCtttt           >  1:4484877/1‑100 (MQ=255)                                                                      aTGCGATTTACATTTTTAAACGATTTAGGAACGTTATTTACTATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCtttt           >  1:1509269/1‑100 (MQ=255)                                                                      aTGCGATTTACATTTTTAAACGATTTAGGAACGTTATTTACTATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCtttt           >  1:4064835/1‑100 (MQ=255)                                                                      aTGCGATTTACATTTTTAAACGATTTAGGAACGTCATTTACTATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCtttt           >  1:3458959/1‑100 (MQ=255)                                                                               aCATTTTTAAACGATTTAGGAACGTTATTTACTATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTagaaagat  >  1:4812434/1‑99 (MQ=255)                                                                               aCATTTTTAAACGATTTAGGAACGTTATTTACTATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTagaaagaa  >  1:3317462/1‑100 (MQ=255)                                                                               aCATTTTTAAACGATTTAGGAACGTTATTTACTATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTagaaagaa  >  1:6369592/1‑100 (MQ=255)                                                                               aCATTTTTAAACGATTTAGGAACGTTATTTACTATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTagaaagaa  >  1:2455009/1‑100 (MQ=255)                                                                               aCATTTTTAAACGATTTAGGAACGTTATTTACTATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTagaaagaa  >  1:983214/1‑100 (MQ=255)                                                                                      |                                                                                             GTGAATAAATAATATGACGTGAAAGGTGCAAAATGTTTTTTTGTGCCTTTCTTTATAGGAGAAAAGACTATGCGATTTACATTTTCAAACGATTTAGGAACGTTATTTACTATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGAA  >  NC_007793/1333860‑1334037

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑