Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,316,107	A→G	T401T (ACA→ACG)	SAUSA300_RS06445 →	aerobic glycerol‑3‑phosphate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,316,107	0	A	G	100.0%	42.2 / NA	18	T401T (ACA→ACG)	SAUSA300_RS06445	aerobic glycerol‑3‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/1);  total (17/1)

ATTGCAGGTGGTAAATTAACAGGCTATCGTCACATGGCTCAAGACATTGTTGATTTAGTATCTAAACGCTTGAAAAAAGACTACGGTTTAACATTTAGTCCATGTAATACAAAAGGTCTGGCAATTTCAGGTGGCGATGTAGGTGGTAGCAAGAACTTTGATGCGTTTGT  >  NC_007793/1316015‑1316184                                                                                             |                                                                              aTTGCAGGTGGTAAATTAACAGGCTATCGTCACATGGCTCAAGACATTGTTGATTTAGTATCTAAACGCTTGAAAAAAGACTACGGTTTAACGTTTAGTc                                                                        >  1:1825445/1‑100 (MQ=255)   tGCAGGTGGTAAATTAACAGGCTATCGTCACATGGCTCAAGACATTGTTGATTTAGTATCTAAACGCTTGAAAAAAGACTACGGTTTAACGTTTAGTCCa                                                                      >  1:1364953/1‑100 (MQ=255)   tGCAGGTGGTAAATTAACAGGCTATCGTCACATGGCTCAAGACATTGTTGATTTAGTATCTAAACGCTTGAAAAAAGACTACGGTTTAACGTTTAGTCCa                                                                      >  1:4560273/1‑100 (MQ=255)   tGCAGGTGGTAAATTAACAGGCTATCGTCACATGGCTCAAGACATTGTTGATTTAGTATCTAAACGCTTGAAAAAAGACTACGGTTTAACGTTTAGTCCa                                                                      >  1:1711827/1‑100 (MQ=255)   tGCAGGTGGTAAATTAACAGGCTATCGTCACATGGCTCAAGACATTGTTGATTTAGTATCTAAACGCTTGAAAAAAGACTACGGTTTAACGTTTAGTCCa                                                                      >  1:4038375/1‑100 (MQ=255)    gCAGGTGGTAAATTAACAGGCTATCGTCACATGGCTCAAGACATTGTTGATTTAGTATCTAAACGCTTGAAAAAAGACTACGGTTTAACGTTTAGTCCAt                                                                     >  1:1567893/1‑100 (MQ=255)       ggtggtAAATTAACAGGCTATCGTCACATGGCTCAAGACATTGTTGATTTAGTATCTAAACGCTTGAAAAAAGACTACGGTTTAACGTTTAGTCCATGTa                                                                  >  1:5480732/1‑100 (MQ=255)          ggtAAATTAACAGGCTATCGTCACATGGCTCAAGACATTGTTGATTTAGTATCTAAACGCTTGAAAAAAGACTACGGTTTAACGTTTAGTCCATGTAATa                                                               >  1:6555928/1‑100 (MQ=255)               cttAACAGGCTATCGTCACATGGCTCAAGACATTGTTGATTTAGTATCTAAACGCTTGAAAAAAGACTACGGTTTAACGTTTAGTCCATGTAATACaaaa                                                          >  1:4672090/2‑100 (MQ=255)                  taCAGGCTATCGTCACATGGCTCAAGACATTGTTGATTTAGTATCTAAACGCTTGAAAAAAGACTACGGTTTAACGTTTAGTCCATGTAATACAAAAGGt                                                       >  1:2079767/2‑100 (MQ=255)                  aaCAGGCTATCGTCACATGGCTCAAGACATTGTTGATTTAGTATCTAAACGCTTGAAAAAAGACTACGGTTTAACGTTTAGTCCATGTAATACAAAAGGt                                                       >  1:4267365/1‑100 (MQ=255)                  aaCAGGCTATCGTCACATGGCTCAAGACATTGTTGATTTAGTATCTAAACGCTTGAAAAAAGACTACGGTTTAACGTTTAGTCCATGTAATACAAAAGGt                                                       >  1:6233189/1‑100 (MQ=255)                     ttgCTATCGTCACATGGCTCAAGACATTGTTGATTTAGTATCTAAACGCTTGAAAAAAGACTACGGTTTAACGTTTAGTCCATGTAATACAAAAGGTCTg                                                    >  1:3385562/3‑100 (MQ=255)                                ccaTGGCTCAAGACATTGTTGATTTAGTATCTAAACGCTTGAAAAAAGACTACGGTTTAACGTTTAGTCCATGTAATACAAAAGGTCTGGCaagatcgga                                         >  1:5238651/2‑93 (MQ=255)                                acaTGGCTCAAGACATTGTTGATTTAGTATCTAAACGCTTGAAAAAAGACTACGGTTTAACGTTTAGTCCATGTAATACAAAAGGTCTGGCAATTTCAgg                                         >  1:1584767/1‑100 (MQ=255)                                        cAAGACATTGTTGATTTAGTATCTAAACGCTTGAAAAAAGACTACGGTTTAACGTTTAGTCCATGTAATACAAAAGGTCTGGCAATTTCAGGCGGCGaag                                 >  1:6434743/1‑98 (MQ=255)                                                                 aaCGCTTGAAAAAAGACTACGGTTTAACGTTTAGTCCATGTAATACAAAAGGCCTGGCAATTTCAGGTGGCGATGTAGGTGGTAGCAAGAACTTTGATGc        >  1:5775702/1‑100 (MQ=255)                                                                       tGAAAAAAGACTACGGTTTAACGTTTAGTCCATGTAATACAAAAGGTCTGGCAATTTCAGGTGGCGATGTAGGTGGTAGCAAGAACTTTGATGCGTTTGt  <  1:5341415/100‑1 (MQ=255)                                                                                             |                                                                              ATTGCAGGTGGTAAATTAACAGGCTATCGTCACATGGCTCAAGACATTGTTGATTTAGTATCTAAACGCTTGAAAAAAGACTACGGTTTAACATTTAGTCCATGTAATACAAAAGGTCTGGCAATTTCAGGTGGCGATGTAGGTGGTAGCAAGAACTTTGATGCGTTTGT  >  NC_007793/1316015‑1316184

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑