Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,295,497	A→G	A207A (GCA→GCG)	SAUSA300_RS06365 →	competence protein CinA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,295,497	0	A	G	100.0%	87.1 / NA	35	A207A (GCA→GCG)	SAUSA300_RS06365	competence protein CinA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/0);  total (35/0)

GAACTATTAAGATTTGCGGGAATAGGTGAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCACCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACAATCATTGATTCAACCTGTTAAAC  >  NC_007793/1295408‑1295588                                                                                          |                                                                                            gAACTATTAAGATTTGCGGGAATAGGTGAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCgg                                                                                   >  1:5976385/1‑100 (MQ=255) gAACTATTAAGATTTGCGGGAATAGGTGAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGACgg                                                                                   >  1:5812354/1‑100 (MQ=255)      gccaaGATTTGCGGGAATAGGTGAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGt                                                                              >  1:1261162/4‑100 (MQ=255)      aTTAAGATTTGCGGGAATAGGTGAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTAGCGGGagat                                                                              >  1:4845687/1‑97 (MQ=255)             attGCGGGAATAGGTGAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAgatcggaag                                                                       >  1:3127924/2‑92 (MQ=255)             agggCGGGAATAGGTGAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAg                                                                       >  1:3620319/4‑100 (MQ=255)               tGCGGGAATAGGTGAGTCTAAAGCAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGttttgatgca                                                                     >  1:1838787/1‑91 (MQ=255)               tGCGGGAATAGGTGAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAgatcggaagag                                                                     >  1:3747588/1‑90 (MQ=255)               tGCGGGAATAGGTGAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGta                                                                     >  1:6054428/1‑100 (MQ=255)               tGCGGGAATAGGTGAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGta                                                                     >  1:1682983/1‑100 (MQ=255)                ccGGGAATAGGTGAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGcat                                                                    >  1:6517942/2‑100 (MQ=255)                     caTAGGTGAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATAtt                                                               >  1:436781/2‑100 (MQ=255)                        aGGTGAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGa                                                            >  1:5558360/1‑100 (MQ=255)                        aGGTGAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGCCGGGAAGTCATGACGTATATATTAGa                                                            >  1:15819/1‑100 (MQ=255)                                cTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGc                                                    >  1:1652091/1‑100 (MQ=255)                                  aaaGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCaa                                                  >  1:2813780/1‑100 (MQ=255)                                                            tATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACaa                        >  1:2714740/1‑100 (MQ=255)                                                            tATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACaa                        >  1:3458625/1‑100 (MQ=255)                                                            tATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACaa                        >  1:1027807/1‑100 (MQ=255)                                                            tATCGATAAACAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGGATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACaa                        >  1:4122009/1‑100 (MQ=255)                                                                 aatggcAGACTAATCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACAATCAtt                   >  1:3708712/6‑100 (MQ=255)                                                                             aTCCTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACAATCATTGATTCAACCTGt       >  1:3780777/1‑100 (MQ=255)                                                                              cccTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACAATCATTGATTCAACCTGtt      >  1:1295626/2‑100 (MQ=255)                                                                               ccTACGATTGCGCCTTTGGCGGGCAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACAATCATTGATTCAACCTGTTa     >  1:4721710/1‑100 (MQ=255)                                                                               ccTACGATTGCGCCTTTGGCGGGCAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACAATCATTGATTCAACCTGTTa     >  1:5317362/1‑100 (MQ=255)                                                                               ccTACGATTGCGCCTTTGGCGGGAAGTCATGGAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACAATCATTGATTCAACCTGTTa     >  1:547251/1‑100 (MQ=255)                                                                               ccTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACAATCATTGATTCAACCTGTTa     >  1:3836945/1‑100 (MQ=255)                                                                               ccTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACAATCATTGATTCAACCTGTTa     >  1:3819102/1‑100 (MQ=255)                                                                               ccTACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACAATCATTGATTCAACCTGTTa     >  1:5014180/1‑100 (MQ=255)                                                                               ccTACGATTGCGCCTATGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACAATCATTGATTCAACCTGTTa     >  1:3292822/1‑100 (MQ=255)                                                                               ccTACGACTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACAATCATTGATTCAACCTGTTa     >  1:1366045/1‑100 (MQ=255)                                                                                 tACGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACAATCATTGATTCAACCTGTTaaa   >  1:206910/1‑100 (MQ=255)                                                                                  aCGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACAATCATTGATTCAACCTGTTAAAc  >  1:2698943/1‑100 (MQ=255)                                                                                  aCGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACAATCATTGATTCAACCTGTTAAAc  >  1:2269884/1‑100 (MQ=255)                                                                                  aCGATTGCGCCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACAATCATTGATTCAACCTGTTAAAc  >  1:1828371/1‑100 (MQ=255)                                                                                          |                                                                                            GAACTATTAAGATTTGCGGGAATAGGTGAATCTAAAGTAGAAACAATATTAATAGATCTTATCGATAAACAGACTAATCCTACGATTGCACCTTTGGCGGGAAGTCATGAAGTATATATTAGATTGACTGCAAATGCCGACTCAAAAGAACAAGCACAATCATTGATTCAACCTGTTAAAC  >  NC_007793/1295408‑1295588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑