Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,272,003	G→C	E839Q (GAA→CAA)	polC →	DNA polymerase III subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,272,003	0	G	C	100.0%	156.0 / NA	61	E839Q (GAA→CAA)	polC	DNA polymerase III subunit alpha
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (61/0);  total (61/0)

TTCCTATTAAAGATGAATTATACACACCGCGTATGGAAGGTGCTAACGAAGAAATTAGAGAACTAAGTTATGCAAATGCGCGTAAACTGTATGGTGAAGACCTGCCTCAAATCGTAATTGATCGATTAGAAAAAGAATTAAAAAGTATTATCGGTAATGGATTTGCGGTAATTTACTTAATTTCGCAA  >  NC_007793/1271908‑1272095                                                                                                |                                                                                             ttCCTATTAAAGATGAATTATACACACCGCGTATGGAAGGTGCTAACGAAGAAATTAGAGAACTAAGTTATGCAAATGCGCGTAAACTGTATGGTCAAGa                                                                                          >  1:1606490/1‑100 (MQ=255)  tCCTATTAAGGATGAATTATACACACCGCGTATGGAAGGTGCTAACGAAGAAATTAGAGAACTAAGATATGCAAATGCGAGTAATCTGTATGGTCAAGat                                                                                         >  1:5772260/1‑99 (MQ=255)  tCCTATTAAAGATGAATTATACACACCGCGTATGGAAGGTGCTAACGAAGAAATTAGAGAACTAAGTTATGCAAATGCGCGTAAACTGTAAGGTCAAGat                                                                                         >  1:4743036/1‑99 (MQ=255)   ccTATTAAAGATGAATTATACACACCGCGTATGGAAGGTGCTAACGAAGAAATTAGAGAACTAAGTTATGCAAATGCGCGTAAACTGTATGGTCAAGAcc                                                                                        >  1:5534598/1‑100 (MQ=255)   ccTATTAAAGATGAATTATACACACCGCGTATGGAAGGTGCTAACGAAGAAATTAGAGAACTAAGTTATGCAAATGCGCGTAAACTGTACGGTCAAGatc                                                                                        >  1:1826932/1‑98 (MQ=255)      aTTAAAGATGAATTATACACACCGCGTATGGAAGGTGCTAACGAAGAAATTAGAGAACTAAGTTATGCAAATGCGCGTAAACTGTATGGCCAAGatcgga                                                                                     >  1:1910707/1‑95 (MQ=255)                    ataCACACCGCGTATGGAAGGTGCTAACGAAGAAATTAGAGAACTAAGTTATGCAAATGCGCGTAAACTGTATGGTCAAGACCTGCCTCAAATCGTAAtt                                                                       >  1:5699139/1‑100 (MQ=255)                     cggccacCGCGTATGGAAGGTGCTAAAGAAGAAATTAGAGAACTAAGTTATGCAAATGCGCGTAAACTGTATGGGCAAGACCTGCCTCAAATAGTAATTg                                                                      >  1:4562583/5‑100 (MQ=255)                      acacacCGCGTATGGAAGGTGCTAACGAAGAAATTAGAGAACTAAGTTATGCAAATGCGCGTAAACTGTATGGTCAAGGCCTGCCTCAAATCGTAATTGa                                                                     >  1:180255/1‑100 (MQ=255)                      acacacCGCGTATGGAAGGTGCTAACGAAGAAATTAGAGAACTAAGTTATGCAAATGCGCGTAAACTGTATGGTCAAGACCTGCCTCAAATCGTAATTGa                                                                     >  1:3584247/1‑100 (MQ=255)                      acacacCGCGTATGGAAGGTGCTAACGAAGAAATTAGAGAACTAAGTTATGCAAATGCGCGTAAACTGTATGGTCAAGACCTGCCTCAAATCGTAATTGa                                                                     >  1:31246/1‑100 (MQ=255)                      acacacCGCGTATGGAAGGTGCTAACGAAGAAATTAGAGAACTAAGTTATGCAAATGCGCGTAAACTGTATGGTCAAGACCTGCCTCAAATCGTAATTGa                                                                     >  1:1930239/1‑100 (MQ=255)                      acacacCGCGTATGGAAGGTGCTAACGAAGAAATTAGAGAACTAAGTTATGCAAATGCGCGTAAACTGTATGGTCAAGACCTGCCTCAAATCGTAATTGa                                                                     >  1:2540613/1‑100 (MQ=255)                      acacacCGCGTATGGAAGGTGCTAACGAAGAAATTAGAGAACTAAGTTATGCAAATGCGCGTAAACTGTATGGTCAAGACCTACCTCAGATCGGaagagc                                                                     >  1:4147651/1‑96 (MQ=255)                      acacacCGCGTATGGAAGGTGCTAACGAAGAAATTAGAGAACTAAGTTATGCAAATGCGCGTAAACTGTATGGTCAAGACCCGCCTCAGATCGGaagagc                                                                     >  1:1549824/1‑96 (MQ=255)                      acacacCGCGTATGGAAGGAGCTAACGAAGAAATTAGAGAACTAAGTTATGCAAATGCGCGTAAACTGTATGGTCAAGACCTGCCTCAAATCGTAATTGa                                                                     >  1:5634481/1‑100 (MQ=255)                       cacacCGCGTATGGAAGGTGCTAACGAAGAAATTAGTGAACTAAGTTATGCAAATGCGCGTAAACTGTATGGTCAAGACCTGCCTCAAATCGTAATTGAt                                                                    >  1:1458523/1‑100 (MQ=255)                       cacacCGCGTATGGAAGGTGCTAACGAAGAAATTAGAGAACTAAGTTATGCAAATGCGCGTAAACTGTATGGTCAAGACCTGCCTCAAATCGTAATTGAt                                                                    >  1:2824693/1‑100 (MQ=255)                        acgccGCGTATGGAAGGTGCTAACGAAGAAATTAGAGAACTAAGTTATGCAAATGCGCGCAAACTGTATGGTCAAAACCTGCCCCAAATCGTAATTGCTc                                                                   >  1:2229566/4‑100 (MQ=255)                        acacCGCGTATGGAAGGTGCTAACGAAGAAATTAGAGAACTAAGTTATGCAAATGCGCGTAAACTGTATGGTCAAGACCTGCCTCAGATCGGaagagcac                                                                   >  1:2134326/1‑94 (MQ=255)                        acacCGCGTATGGAAGGTGCTAACGAAGAAATTAGAGAACTAAGTTATGCAAATGCGCGTAAACTGTATGGTCAAGACCTGCCTCAAATCGTAATTGATc                                                                   >  1:5782699/1‑100 (MQ=255)                        acacCGCGTATGGAAGGTGCTAACGAAGAAATTAGAGAACTAAGTTATGCAAATGCGCGTAAACTGTATGGTCAAGACCTGCCTCAAATCGTAATTGATc                                                                   >  1:2944702/1‑100 (MQ=255)                        acacCGCGTATGGAAGGTGCTAACGAAGAAATTAGAGAACTAAGTTATGCAAATGCGCGTAAACTGTATGGTCAAGACCTGCCTCAAATCGTAAGAGATc                                                                   >  1:2252591/1‑100 (MQ=255)                         tacCGCGTATGGAAGGTGCTAACGAAGAAATTAGAGAACTAAGTTATGCAAATGCGCGTAAACTGTATGGTCAAGACCTGCCTCAAATCGTAATTGATCg                                                                  >  1:4498320/2‑100 (MQ=255)                         tacCGCGTATGGAAGGTGCTAACGAAGAAATTAGAGAACTAAGTTATGCAAATGCGCGTAAACTGTATGGTCAAGACCTGCCTCAAATCGTAATTGATCg                                                                  >  1:4318907/2‑100 (MQ=255)                         tacCGCGTATGGAAGGCGCTAACGAAGAAATTAGAGAACTAAGTTATGCAAATGCGCGTAAACTGTATGGTCAAGACCTGCCTCAAATCGTAATTGATCg                                                                  >  1:1699277/2‑100 (MQ=255)                         cacCGCGTATGGAAGGTGCTAACGAAGAAATTAGAGAACTAAGTTATGCAAATGCGCGTAAACTGTATGGTCAAGACCTGCCTCAAATCGTAATTGATCg                                                                  >  1:3412103/1‑100 (MQ=255)                         cacCGCGTATGGAAGGTGCTAACGAAGAAATTAGAGAACTAAGTTATGCAAATGCGCGTAAACTGTATGGTCAAGACCTGCCTCAAATCGTAACAGATCg                                                                  >  1:3274854/1‑100 (MQ=255)                         aacCGCGTATGGAAGGTGCTAACGAAGAAATTAGAGAACTAAGTTATGCAAATGCGCGTAAACTGTATGGTCAAGACCTGCCTCAAATCGTAATTGATCg                                                                  >  1:5676627/2‑100 (MQ=255)                          acCGCGTATGGAAGGTGCTAACGAAGAAATTAGAGAACTAAGTTATGCAAATGCGCGTAAACTGTATGGTCAAGACCTGCCTCAAATCGTAATTGATCGa                                                                 >  1:3595456/1‑100 (MQ=255)                          acCGCGTATGGAAGGTGCTAACGAAGAAATTAGAGAACTAAGTTATGCAAATGCGCGTAAACTGTATGGTCAAGACCTGCCTCAAATCGTAATTGATCGa                                                                 >  1:2684070/1‑100 (MQ=255)                          acCGCGTATGGAAGGTGCTAACGAAGAAATTAGAGAACTAAGTTATGCAAATGCGCGTAAACTGTATGGTCAAGACCTGCCTCAAATCGTAATTGATCGa                                                                 >  1:4570816/1‑100 (MQ=255)                           ccGCGTATGGAAGGTGCTAACGAAGAAATTAGAGAACTAAGTTATGCAAATGCGCGTAAACTGTATGGTCAAGACCTGCCTCAAATCGTAATTGATCGAt                                                                >  1:1072272/1‑100 (MQ=255)                           ccGCGTATGGAAGGTGCTAACGAAGAAATTAGAGAACTAAGTTATGCAAATGCGCGTAAACTGTATGGTCAAGACCTGCCTCAAATCGTAATTGATCGAt                                                                >  1:1511896/1‑100 (MQ=255)                           acaggTATGGAAGGTGCTAACGAAGAAATTAGAGAACTAAGTTATGCAAATGCGCGTAAACTGTATGGTCAAGACCTGCCTCAAATCGTAATAGATCgga                                                                >  1:3054334/5‑98 (MQ=255)                                 aTGGAAGGTGCTAACGAAGACATTAGAGAACTAAGTTATGCAAATGCGCGTAAACTGTATGGTCAAGACCTGCCTCAAATCGTAATTGATCGATTAGaaa                                                          >  1:4942872/1‑100 (MQ=255)                                    caaGGTGCTAACGAAGAAATTAGAGCACTAAGTTATGCAAATGCGCGTAAACTGTATGGTCAAGACCTGCCTCAAATCGTAATTGCTCGATTAGAAAAAg                                                       >  1:4967324/2‑100 (MQ=255)                                     taGGTGCTAACGAAGAAATTAGAGAACTAAGTTATGCAAATGCGCGTAAACTGTATGGTCAAGACCTGCCTCAAATCGTAATTGATCGATTAGAAAAAGa                                                      >  1:5142372/2‑100 (MQ=255)                                     caGGTGCTAACGAAGAAATTAGAGAACTAAGTTATGCAAATGCGCGTAAACTGTATGGTCAAGACCTGCCTCAAATCGTAATTGATCGATTAGAAAAAGa                                                      >  1:4277341/2‑100 (MQ=255)                                      aGGTGCTAACGAAGAAATTAGAGAACTAAGTTATGCAAATGCGCTTAAACTGTATGGTCAAGACCTGCCTCAAATCGTAATTGATCGATTAGAAAAAGaa                                                     >  1:969214/1‑100 (MQ=255)                                         tGCTAACGAAGAAATTAGAGAACTAAGTTATGCAAATGCGCGTAAACTGTATGGTCAAGACCTGCCTCAAATCGTAATTGATCGATTAGAAAAAGAATTa                                                  >  1:5174059/1‑100 (MQ=255)                                          gCTAACGAAGAAATTAGAGAACTAAGTTATGCAAATGCGCGTAAACTGTATGGTCAAGACCTGCCTCAAATCGTAATTGATCGATTAGAAAAAGAATTaa                                                 >  1:630239/1‑100 (MQ=255)                                          gCTAACGAAGAAATTAGAGAACTAAGTTATGCAAATGCGCGTAAACTGTATGGTCAAGACCTGCCTCAAATCGTAATTGATCGATTAGAAAAAGAATTaa                                                 >  1:1676599/1‑100 (MQ=255)                                          gCTAACGAAGAAATTAGAGAACTAAGTTATGCAAATGCGCGTAAACTGTATGGTCAAGACCTGCCTCAAATCGTAATTGATCGATTAGAAAAAGAATTaa                                                 >  1:6601926/1‑100 (MQ=255)                                            tAACGAAGAAATTAGAGAACTAAGTTATGCAAATGCGCGTAAACTGTATGGTCAAGACCTGCCTCAAATCGGAATTGATCGATTAGAAAAAGAATTaaaa                                               >  1:5604696/1‑100 (MQ=255)                                             gaCGAAGAAATTAGAGAACTAAGTTATGCAAATGCGCGTAAACTGTATGGTCAAGACCTGCCTCAAATCGTAATTGATCGATTAGAAAAAGAATTaaaaa                                              >  1:695867/2‑100 (MQ=255)                                             aaCGAAGAAATTAGAGAACTAAGTTATGCAAATGCGCGTAAACTGTATGGTCAAGACCTGCCTCAAATCGTAATTGATCGATTAGAAAAAGAATTaaaaa                                              >  1:5358780/1‑100 (MQ=255)                                                   gaaATTAGAGAACTAAGTTATGCAAATGCGCGTAAACTGTATGGTCAAGACCTGCCTCAAATCGTAATTGATCGATTAGAAAAAGAATTAAAAAGtatta                                        >  1:996705/1‑100 (MQ=255)                                                                 aaGTTATGCAAATGCGCGTAAACTGTATGGTCAAGACCTGCCTCAAATCGTAATTGATCGATTAGAAAAAGAATTAAAAAGTAAGATCggaagagcacac                          >  1:2482158/1‑90 (MQ=255)                                                                     tATGCAAATGCGCGTAAACTGTATGGTCAAGACCTGCCTCAAATCGTAATTGATCGATTAGAAAAAGAATTAAAAAGTATTATCGGTAATGGATTTGCgg                      >  1:4579753/1‑100 (MQ=255)                                                                     tATGCAAATGCGCGTAAACTGTATGGTCAAGACCTGCCTCAAATCGTAATTGATCGATTAGAAAAAGAATTAAAAAGGAATATCGGTAATGGATTTGCgg                      >  1:848011/1‑100 (MQ=255)                                                                        gCAAATGCGCGTAAACTGTATGGTCAAGACCTGCCTCAAATCGAAATTGATCGATTAGAAAAAGAATTAAAAAGTATTATCGGTAATGGATTTGCGGTaa                   >  1:2097286/1‑100 (MQ=255)                                                                          caaTGCGCGTAAACTGTATGGTCAAGACCTGCCTCAAATCGTAATTGATCGATTAGAAAAAGAATTAAAAAGTATTATCGGTAATGGATTTGCGGTAAtt                 >  1:974893/2‑100 (MQ=255)                                                                                    aaaCTGTATGGTCAAGACCTGCCTCAAATCGTAATTGATCGATTAGAAAAAGAATTAAAAAGTATTATCGGTAATGGATTTGCGGTAATTTACTTAAttt       >  1:5916776/1‑100 (MQ=255)                                                                                    aaaCTGTATGGTCAAGACCTGCCTCAAATCGTAATTGATCGATTAGAAAAAGAATTAAAAAGTATTATCGGTAATGGATTTGCGGTAATTTACTTAAttt       >  1:6086161/1‑100 (MQ=255)                                                                                     cccTGTATGGTCAAGACCTGCCTCAAATCGTAATTGATCGATTAGAAAAAGAATTAAGAAGTATTATCGGTAATGGATTTGCGGTAATTTACTTAATTTc      >  1:1300010/3‑100 (MQ=255)                                                                                      aCTGTATGGTCAAGACCTGCCTCAAATCGTAATTGATCGATTAGAAAAAGAATTAAAAAGTATTATCGGTAATGGATTTGCGGTAGTTTACTTAATTTCg     >  1:4912702/1‑100 (MQ=255)                                                                                         gTATGGTCAAGACCTGCCTCAAATCGTAATTGATCGATTAGCAAAAGAATTAAAAAGTATTATCGGTAATGGATTTGCGGTAATTTACTTAATTTCGCaa  >  1:5733640/1‑100 (MQ=255)                                                                                         gTATGGTCAAGACCTGCCTCAAATCGTAATTGATCGATTAGAAAAAGAATTAAAAAGTATTATCGGTAATGGATTTGCGGTAATTTACTTAATTTCGCaa  >  1:6212920/1‑100 (MQ=255)                                                                                         gTATGGTCAAGACCTGCCTCAAATCGTAATTGATCGATTAGAAAAAGAATTAAAAAGCATTATCGGTAATGGATTTGCGGTAATTTACTTAATTTCGCaa  >  1:4061391/1‑100 (MQ=255)                                                                                         ccaTGGTCAAGACCTGCCTCAAATCGTAATTGATCGATTAGAAAAAGAATTAAAAAGTATTATCGGTAATGGATTTGCGGTAATTTACTTAATTTCGCaa  >  1:5799441/3‑100 (MQ=255)                                                                                                |                                                                                             TTCCTATTAAAGATGAATTATACACACCGCGTATGGAAGGTGCTAACGAAGAAATTAGAGAACTAAGTTATGCAAATGCGCGTAAACTGTATGGTGAAGACCTGCCTCAAATCGTAATTGATCGATTAGAAAAAGAATTAAAAAGTATTATCGGTAATGGATTTGCGGTAATTTACTTAATTTCGCAA  >  NC_007793/1271908‑1272095

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑