Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,269,542	A→T	I18I (ATA→ATT)	polC →	DNA polymerase III subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,269,542	0	A	T	100.0%	205.3 / NA	71	I18I (ATA→ATT)	polC	DNA polymerase III subunit alpha
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (71/0);  total (71/0)

CGAACACGTAAAAGTAATTTCTATTTAAGGTGGTAATTGTCTTGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATATCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGTATAGATGTTTCTAACAAAAACAGAACATGGGAAT  >  NC_007793/1269448‑1269624                                                                                               |                                                                                   tgAACACGTAAAAGTAATTTCTATTTAAGGTGGTAATTGTCTTGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATttcaaa                                                                               >  1:4784182/2‑100 (MQ=255) cGAACACGTAAAAGTAATTTCTATTTAAGGTGGTAATTGTCTTGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAGTttcaaa                                                                               >  1:2662252/1‑100 (MQ=255)                   atCTATTTAAGGTGGTAATTGTCTTGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAAt                                                             >  1:1371746/2‑100 (MQ=255)                     cTATTTAAGGTGGTAATTGTCTTGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAAttt                                                           >  1:1805027/1‑100 (MQ=255)                       aTTTAAGGTGGTAATTGTCTTGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTa                                                         >  1:6567039/1‑100 (MQ=255)                       aTTTAAGGTGGTAATTGTCTTGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTa                                                         >  1:4360407/1‑100 (MQ=255)                           aaGGTGGTAATTGTCTTGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAAtt                                                     >  1:3216427/1‑100 (MQ=255)                                        tactGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGac                                        >  1:1676825/4‑100 (MQ=255)                                        ccTTGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGac                                        >  1:3367570/2‑100 (MQ=255)                                        agctGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAGCTGac                                        >  1:781212/4‑100 (MQ=255)                                         tttGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGaca                                       >  1:972543/2‑100 (MQ=255)                                         cTTGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGaca                                       >  1:4765967/1‑100 (MQ=255)                                         attGGCAATGACGGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGaca                                       >  1:6377571/2‑100 (MQ=255)                                         atcggCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGaca                                       >  1:2311223/4‑100 (MQ=255)                                          gtGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGacac                                      >  1:4572483/2‑100 (MQ=255)                                          ctGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGacac                                      >  1:1122770/2‑100 (MQ=255)                                          ctGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGacac                                      >  1:2280746/2‑100 (MQ=255)                                           tGGCAATGACAGAGCAACAAAAATTTAACGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACg                                     >  1:6251570/1‑100 (MQ=255)                                           tGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCCGGTGAACTGACACg                                     >  1:4851334/1‑100 (MQ=255)                                           tGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACg                                     >  1:4431542/1‑100 (MQ=255)                                           tGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACg                                     >  1:1416892/1‑100 (MQ=255)                                           tGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACg                                     >  1:4784264/1‑100 (MQ=255)                                           tGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACg                                     >  1:3113650/1‑100 (MQ=255)                                           tGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACg                                     >  1:4285435/1‑100 (MQ=255)                                           tGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACg                                     >  1:5178200/1‑100 (MQ=255)                                           tGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACg                                     >  1:6195769/1‑100 (MQ=255)                                           tGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACg                                     >  1:4510414/1‑100 (MQ=255)                                           tGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACg                                     >  1:3462517/1‑100 (MQ=255)                                           tGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACg                                     >  1:6273745/1‑100 (MQ=255)                                           tGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACg                                     >  1:3347167/1‑100 (MQ=255)                                           tGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACg                                     >  1:1925119/1‑100 (MQ=255)                                           tGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACg                                     >  1:2970474/1‑100 (MQ=255)                                           tGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACg                                     >  1:2893195/1‑100 (MQ=255)                                           tGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACg                                     >  1:6635584/1‑100 (MQ=255)                                           tGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACg                                     >  1:2677671/1‑100 (MQ=255)                                           tGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACg                                     >  1:2533230/1‑100 (MQ=255)                                           tGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACg                                     >  1:2394198/1‑100 (MQ=255)                                           tGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACg                                     >  1:2318910/1‑100 (MQ=255)                                           tGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACg                                     >  1:942006/1‑100 (MQ=255)                                           tGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACg                                     >  1:2028407/1‑100 (MQ=255)                                           tGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACg                                     >  1:1091121/1‑100 (MQ=255)                                           tGGCAATGACAGAGCAACAAAAATTTAAAGGGCTTGCTGAGCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTGAAATTCAGTTGAACTGACACg                                     >  1:4672387/1‑100 (MQ=255)                                           tGGCAATGACAGAGCAACAAAAATTTAAAGCGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGTAATTTTAAATTCAGGTGAACTGACACg                                     >  1:4771142/1‑100 (MQ=255)                                           cggCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACg                                     >  1:5212709/2‑100 (MQ=255)                                            ggCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGt                                    >  1:2010518/1‑100 (MQ=255)                                            ggCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGt                                    >  1:4361167/1‑100 (MQ=255)                                            ggCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGt                                    >  1:2209957/1‑100 (MQ=255)                                            ggCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGt                                    >  1:6427498/1‑100 (MQ=255)                                            ggCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGt                                    >  1:3112192/1‑100 (MQ=255)                                            ggCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAA‑TTTAAATTCAGGTGAACTGACACGta                                   >  1:438374/1‑100 (MQ=255)                                               caTGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGtata                                 >  1:4217986/2‑100 (MQ=255)                                               aaTGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGtata                                 >  1:5413992/1‑100 (MQ=255)                                                aTGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGGACTGACGCGTATAg                                >  1:4955614/1‑100 (MQ=255)                                                aTGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGGACTAACACGTATAg                                >  1:4991548/1‑100 (MQ=255)                                                aTGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGTATAg                                >  1:4050778/1‑100 (MQ=255)                                                aTGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGTATAg                                >  1:5406520/1‑100 (MQ=255)                                                  gACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGTATAGAt                              >  1:1600139/1‑100 (MQ=255)                                                  gACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGTATAGAt                              >  1:5920941/1‑100 (MQ=255)                                                      ctgCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGTATAGATGttt                          >  1:1447414/3‑100 (MQ=255)                                                      atgCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGTATAGATGttt                          >  1:1405786/3‑100 (MQ=255)                                                         tgacaaAAATTTAAAGAGCTTGCTGATCAAATTAAAATTTCAAATCAATTCGATGCTGAACTTTTAAATTCAGGTGAACTGACACGTATAGATGTTTCTa                       >  1:5394417/3‑100 (MQ=255)                                                          aacaaAAATTTAAAGTGATTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGAGAACTGACACGTATAGATGTTTCTaa                      >  1:6595948/1‑100 (MQ=255)                                                           tcaaAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGTATAGATGTTTCTAAc                     >  1:6377622/2‑100 (MQ=255)                                                                          gcttgctGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGTATAGATGTTTCTAACAAAAACAGAACATgg      >  1:3670295/1‑100 (MQ=255)                                                                          gcttgctGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGTATAGATGTTTCTAACAAAAACAGAACATgg      >  1:915036/1‑100 (MQ=255)                                                                           cttgctGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGTATAGATGTTTGTAACAGAAACAGAACagatc     >  1:2001866/1‑96 (MQ=255)                                                                           cttgctGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGTATAGATGTTTCTAACACAAACAGAACagatc     >  1:4819278/1‑96 (MQ=255)                                                                             tgctGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGTATAGATGTTTCTAACAAAAACAGAACATGGGag   >  1:1948644/1‑99 (MQ=255)                                                                             tgctGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGTATAGATGTTTCTAACAAAAACAGAACATGGGaa   >  1:543910/1‑100 (MQ=255)                                                                             tgctGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGTATAGATGTTTCTAACAAAAACAGAACATGGGaa   >  1:5255314/1‑100 (MQ=255)                                                                              gctGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGTATGGATGTTTCTAACAAAAACAGAACATGGGAAt  >  1:4676547/1‑100 (MQ=255)                                                                              gctGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGTATAGATGTTTCTAACAAAAACAGatcggaagagc  >  1:277909/1‑90 (MQ=255)                                                                              gctGATCAAATTAAAATTTCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGTATAGATGTTTCTAACAAAAACAGAACATGGGAAt  >  1:394347/1‑100 (MQ=255)                                                                                               |                                                                                   CGAACACGTAAAAGTAATTTCTATTTAAGGTGGTAATTGTCTTGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATATCAAATCAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGTATAGATGTTTCTAACAAAAACAGAACATGGGAAT  >  NC_007793/1269448‑1269624

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑