Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,251,223	C→T	P308L (CCT→CTT)	SAUSA300_RS06175 →	methicillin resistance protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,251,223	0	C	T	100.0%	11.6 / NA	6	P308L (CCT→CTT)	SAUSA300_RS06175	methicillin resistance protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/0);  total (6/0)

CAAAATTAAATCAGTTAAATATGCAATTATCTAGTATTAATAATAGAATTAGTAAAACCGAAGAACTAATATTTGAAGATGGACCTGTTTTGGATTTAGCTGCTGCTTTATTTATATGTACTGATGATGAAGTTTATTATCTATCAAGTGGATCAAATCCGAAATATAATCAGTATATGG  >  NC_007793/1251139‑1251318                                                                                     |                                                                                                cAAAATTAAATCAGTTAAATATGCAATTATCTAGTATTAATAATAGAATTAGTAAGACCGAAGAACTAATATTTGAAGATGGACTTGTTTTGGATTTAgc                                                                                  >  1:816221/1‑100 (MQ=255)                     atGCAATTATCTAGTATTAATAATAGAATTAGTAAAACCGAAGAACTAATATTTGAAGATGGACTTGTTTTGGATTTAGCTGCTGCTTTATTTATATgaa                                                              >  1:5397583/1‑98 (MQ=255)                                                     gtgtaCCGAAGAACTAATATTTGAAGATGGACTTGTTTTGGATTTAGCTGCTGCTTTATTTATATGTACTGATGATGAAGTTTATTATCTATCAAGTGGa                              >  1:5316014/5‑100 (MQ=255)                                                       aagccGAAGAACTAATATTTGAAGATGGACTTGTTTTGGATTTAGCTGCTGCTTTATTTATATGTACTGATGATGAAGTTTATTATCTATCAAGTGGATc                            >  1:5368839/4‑100 (MQ=255)                                                              agaaCTAATATTTGAAGATGGACTTGTTTTGGATTTAGCTGCTGCTTTATTTATATGTACTGTTGATGAAGTTTATTATCTATCAAGTGGATCAAATCCg                     >  1:811697/1‑100 (MQ=255)                                                                                 ggACTTGTTTTGGATTTAGCTGCTGCTTTATTTATATGTACTGATGATGAAGTTTATTATCTATCAAGTGGATCAAATCCGAAATATAATCAGTATATgg  >  1:917661/1‑100 (MQ=255)                                                                                     |                                                                                                CAAAATTAAATCAGTTAAATATGCAATTATCTAGTATTAATAATAGAATTAGTAAAACCGAAGAACTAATATTTGAAGATGGACCTGTTTTGGATTTAGCTGCTGCTTTATTTATATGTACTGATGATGAAGTTTATTATCTATCAAGTGGATCAAATCCGAAATATAATCAGTATATGG  >  NC_007793/1251139‑1251318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑