Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,185,383	C→T	intergenic (+57/‑26)	SAUSA300_RS05875 → / → SAUSA300_RS05880	cell division protein/S4 RNA‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,185,383	0	C	T	100.0%	83.1 / NA	32	intergenic (+57/‑26)	SAUSA300_RS05875/SAUSA300_RS05880	cell division protein/S4 RNA‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/0);  total (32/0)

TACTCCAAATCTTTAATTGGATTTTAATTCAATTACAATAAATTAAGCGACATAACGAGGCAAGGGTACATGATACTTTAGCCTCGTTTTTGATATGCATTTTTCTGAATATAAGGGCAATAGATGGTATTTTATAATTTTTTTAAGGTAGTGATTAACATAGATATTTATCAACACTTTAGACAGGAAGAATA  >  NC_007793/1185286‑1185479                                                                                                  |                                                                                                 tACTCCAAATCTTTAATTGGATTTTAATTCAATTACAATAAATTAAGCGACATAACGAGGCAAGGGGACATGATATTTTAGCCTCGTTTTTGATATGTAt                                                                                                >  1:1726654/1‑100 (MQ=255) caCTCCAAATCTTTAATTGGATTTTAATTCAATTACAATAAATTAAGCGACATAACGAGGCAAGGGTACATGATACTTTAGCCTCGTTTTTGATATGTAt                                                                                                >  1:6075181/2‑100 (MQ=255)  aCTCCAAATCTTTAATTGGATTTTAATTCAATTACAATAAATTAAGCGACATAACGAGGCAAGGGTACATGATACTTTAGCCTCGTTTTTGATATGTAtt                                                                                               >  1:5691737/1‑100 (MQ=255)     ccAAATCTTTAATTGGATTTTAATTCAATTACAATAAATTAAGCGACATAACGAGGCAAGGGTACATGATACTTTAGCCTCGTTTTTGATATGTAttttt                                                                                            >  1:5615712/1‑100 (MQ=255)             ttAATTGGATTTTAATTCAATTACAATTAATTAAGCGCCATAACGAGGCAAGGGTACATGCTACTTTATCCTCGTTTTTGATATGTATTTTTCTGAatat                                                                                    >  1:3895191/1‑100 (MQ=255)             ttAATTGGATTTTAATTCAATTACAATTAATTAAGCGACATATCGAGGCAAGGGCACATGATACTTTAGCCTTGTTTTTGATATGTATTCTTCTGAatat                                                                                    >  1:1238507/1‑100 (MQ=255)                  tGGATTTTAATTCAATTACAATAAATTAAGCGACATAACGAGGCAAGGGTACATGATACTTTAGCCTCGTTTTTGATATGTATTTTTCTGAATATAAggg                                                                               >  1:2426688/1‑100 (MQ=255)                        ttAATTCAATTACAATAAATTAAGCGACATAACGAGGCAAGGGTACATGATACTTTAGCCTCGTTTTTGATATGTATTTTTCTGAATATAAGGGCAATAg                                                                         >  1:3413436/1‑100 (MQ=255)                                             aaGCGACATAACGAGGCAAGGGTACATGATACTTTAGCCTCGTTTTTGATATGTATTTTTCTGAATATAAGGGCAATAGATGGTATTTTATAAttttttt                                                    >  1:4579934/1‑100 (MQ=255)                                                      aaCGAGGCAAGGGTACATGATACTTTAGCCTCGTTTTTGATATGTATTTTTCTGAATATAAGGGCAATAGATGGTATTTTATAATTTTTTTAAGGTAGTg                                           >  1:4589004/1‑100 (MQ=255)                                                              caGGGTACATGATACTTTAGCCTCGTTTTTGATATGTATTTTTCTGAATATAAGGGCAATAGATGGTATTTTATAATTTTTTTAAGGTAGTGATTAACAt                                   >  1:3063587/2‑100 (MQ=255)                                                               aGGGTACATGATACTTTAGCCTCGTTTTTGATATGTATTTTTCTGAATATAAGGGCAATAGATGGTATTTTATAATTTTTTTAAGGTAGTGATTAACATa                                  >  1:2324743/1‑100 (MQ=255)                                                                gggTACATGATACTTTAGCCTCGTTTTTGCTATGTATTTTTCTGAATATAAGGGCACTAGATGGTATTTTATAATTTTTTTAAGGTAGTGATTAACATAg                                 >  1:3490811/1‑100 (MQ=255)                                                                    aCATGATACTTTAGCCTCGTTTTTGATATGTATTTTTCTGAATATAAGGGCAATAGATGGTATTTTATAATTTTTTTAAGGTAGTGATTAACATAGatat                             >  1:2925480/1‑100 (MQ=255)                                                                      aTGATACTTTAGCCTCGTTTTTGATATGTATTTTTCTGAATATAAGGGCAATAGATGGTATTTTATAATTTTTTTAAGGTAGTGATTAACATAGATAttt                           >  1:2070423/1‑100 (MQ=255)                                                                      aTGATACTTTAGCCTCGTTTTTGATATGTATTTTTCTGAATATAAGGGCAATAGATGGTATTTTATAATTTTTTTAAGGTAGTGATTAACATAGATAttt                           >  1:340409/1‑100 (MQ=255)                                                                             tttCGCCTCGTTTTTGATATGTATTTTTCTGATTCTAAGGGCCCTAGATGGTATTTTATAATTTTTTTAAGGTAGTGATTATCATAGATATTTATCAaca                    >  1:2555823/1‑100 (MQ=255)                                                                             tttAGCCTCGTTTTTGATATGTATTTTTCTGAATATAAGGGCAATAGATGGTATTTTATAATTTTTTTAAGGTAGTGATTAACATAGATATTTATCAacg                    >  1:4191448/1‑99 (MQ=255)                                                                             tttAGCCTCGTTTTTGATATGTATTTTTCTGAATATAAGGGCAATAGATGGTATTTTATAATTTTTTTAAGGTAGTGATTAACATAGATATTTATCAaca                    >  1:2611581/1‑100 (MQ=255)                                                                              ttAGCCTCGTTTTTGATATGTATTTTTCTGAATATAAGGGCAATAGATGGTATTTTATAATTTTTTTAAGGTAGTGATTAACATAGATATTTATCAacac                   >  1:5810052/1‑100 (MQ=255)                                                                              ttAGCCTCGTTTTTGATATGTATTTTTCTGAATATAAGGGCAATAGATGGTATTTTATAATTTTTTTAAGGTAGTGATTAACATAGATATTTATCAacac                   >  1:359928/1‑100 (MQ=255)                                                                              ttAGCCTCGTTTTTGATATGTATTTTTCTGAATATAAGGGCAATAGATGGTATTTTATAATTTTTTTAAGGTAGTGATTAACATAGATATTTATCAacac                   >  1:1200794/1‑100 (MQ=255)                                                                              ttAGCCTCGTTTTTGATATGTATTTTTCTGAATATAAGGGCAATAGATGGTATTTTATAATTTTTTTAAGGTAGTGATTAACATAGATATTTATCAacaa                   >  1:5164367/1‑99 (MQ=255)                                                                               tAGCCTCGTTTTTGATATGTATTTTTCTGAATATAAGGGCAATAGATGGTATTTTATAATTTTTTTAAGGTAGTGATTAACATAGATATTTATCAACCCt                  >  1:3616833/1‑100 (MQ=255)                                                                               tAGCCTCGTTTTTGATATGTATTTTTCTGAATATAAGGGCAATAGATGGTATTTTATAATTTTTTTAAGGTAGTGATTAACATAGATATTTATCAACACt                  >  1:218510/1‑100 (MQ=255)                                                                                aGCCTCGTTTTTGATATGTATTTTTCTGAATATAAGGGCAATAGATGGTATTTTATAATTTTTTTAAGGTAGTGATTAACATAGATATTTATCAACACtt                 >  1:3167621/1‑100 (MQ=255)                                                                                  ccTCGTTTTTGATATGTATTTTTCTGAATATAAGGGCAATAGATGGTATTTTATAATTTTTTTAAGGTAGTGATTAACATAGATATTTATCAACACCTTa               >  1:4116387/1‑100 (MQ=255)                                                                                     cGTTTTTGATATGTATTTTTCTGAATATAAGGGCAATAGATGGTATTTTATAATTTTTTTAAGGTAGTGATTAACATAGATATTTATCAacacccagaga            >  1:1688554/1‑93 (MQ=255)                                                                                         tttGATATGTATTTTTCTGAATATAAGGGCAATAGATGGTATTTTATAATTTTTTTAAGGTAGTGATTAACATAGATATTTATCAACACTTTAGatcgga        >  1:4073203/1‑95 (MQ=255)                                                                                         tttGATATGTATTTTTCTGAATATAAGGGCAATAGATGGTATTTTATAATTTTTTTAAGGTAGTGATTAACATAGATATTTATCAACACTTTAGACAGga        >  1:5773932/1‑100 (MQ=255)                                                                                         tttGATATGTATTTTTCTGAATATAAGGGCAATAGATGGTATTTTATAATTTTTTTAAGGTAGTGATTAACATAGATATTTATCAACACTTTAGACAGga        >  1:2475332/1‑100 (MQ=255)                                                                                               atGTATTTTTCTGAATATAAGGGCAATAGATGGTATTTTATAATTTTTTTAAGGTAGTGATTAACATAGATATTTATCAACACTTTAGACAGGAAGAATa  >  1:1107084/1‑100 (MQ=255)                                                                                                  |                                                                                                 TACTCCAAATCTTTAATTGGATTTTAATTCAATTACAATAAATTAAGCGACATAACGAGGCAAGGGTACATGATACTTTAGCCTCGTTTTTGATATGCATTTTTCTGAATATAAGGGCAATAGATGGTATTTTATAATTTTTTTAAGGTAGTGATTAACATAGATATTTATCAACACTTTAGACAGGAAGAATA  >  NC_007793/1185286‑1185479

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑