Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,170,956	C→T	A224V (GCA→GTA)	SAUSA300_RS05810 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,170,956	0	C	T	98.7%	203.2 / NA	76	A224V (GCA→GTA)	SAUSA300_RS05810	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (73/1);  total (75/1)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACACAAGGTCTACTGGAGATTTGTGACAGAATTATTGACCAATATGACTCGTGGACTGATATGTTTAAAGCACTACTGCATGAAACATTTAAAGCATATGGCGTTCTATTTATAGATGCGCAGTTTGAGCCGTTAAGAAAAATGGAAGCGCCTATGTTTAAAAAGATTTTGAAAAAACATC  >  NC_007793/1170862‑1171042                                                                                               |                                                                                       acacAAGGTCTACTGGAGATTTGTGACAGAATTATTGACCAATATGACTCGTGGACTGATATGTTTAAAGCACTACTGCATGAAACATTTAAAGTATATg                                                                                   >  1:5515146/1‑100 (MQ=255)            aCTGGAGATTTGTGACAGAATTATTGACCAATATGACTCGTGGACTGATATGTTTAAAGCACTACTGCATGAAACATTTAAAGTATATGGctagatcgga                                                                        >  1:1662822/1‑91 (MQ=255)            aCTGGAGATTTGTGACAGAATTATTGACCAATATGACTCGTGGACTGATATGTTTAAAGCACTACTGCATGAAACATTTAAAGTATATGGcagatcggaa                                                                        >  1:4039281/1‑91 (MQ=255)            aCTGGAGATTTGTGACAGAATTATTGACCAATATGACTCGTGGACTGATATGTTTAAAGCACTACTGCATGAAACATTTAAAGTATATGGCGTTCTAttt                                                                        >  1:4455436/1‑100 (MQ=255)            aCTGGAGATTTGTGACAGAATTATTGACCAATATGACTCGTGGACTGATATGTTTAAAGCACTACTGCATGAAACATTTAAAGTATATGGCGTTCTAttt                                                                        >  1:3436082/1‑100 (MQ=255)            aCTGGAGATTTGTGACAGAATTATTGACCAATATGACTCGTGGACTGATATGTTTAAAGCACTACTGCATGAAACATTTAAAGTATATGGCGTTCTAttt                                                                        >  1:4472256/1‑100 (MQ=255)            aCTGGAGATTTGTGACAGAATTATTGACCAATATGACTCGTGGACTGATATGTTTAAAGCACTACTGCATGAAACATTTAAAGTATATGGCGTTCTAttt                                                                        >  1:273915/1‑100 (MQ=255)            aCTGGAGATTTGTGACAGAATTATTGACCAATATGACTCGTGGACTGATATGTTTAAAGCACTACTGCATGAAACATTTAAAGTATATGGCGTTCTAttt                                                                        >  1:25361/1‑100 (MQ=255)            aCTGGAGATTTGTGACAGAATTATTGACCAATATGACTCGTGGACTGATATGTTTAAAGCACTACTGCATGAAACATTTAAAGTATATGGCGTTCTAttt                                                                        >  1:5395138/1‑100 (MQ=255)            aCTGGAGATTTGTGACAGAATTATTGACCAATATGACTCGTGGACTGATATGTTTAAAGCACTACTGCATGAAACATTTAAAGTATATGGCGTTCTAttt                                                                        >  1:3654691/1‑100 (MQ=255)            aCTGGAGATTTGTGACAGAATTATTGACCAATATGACTCGTGGACTGATATGTTTAAAGCACTACTGCATGAAACATTTAAAGTATATGGCGTTCTAttt                                                                        >  1:5454175/1‑100 (MQ=255)            aCTGGAGATTTGTGACAGAATTATTGACCAATATGACTCGTGGACTGATATGTTTAAAGCACTACTGCATGAAACATTTAAAGTATATGGCGTTCTAttt                                                                        >  1:5774092/1‑100 (MQ=255)            aCTGGAGATTTGTGACAGAATTATTGACCAATATGACTCGTGGACTGATATGTTTAAAGCACTACTGCATGAAACATTTAAAGTATATGGCGTTCTAttt                                                                        >  1:5832203/1‑100 (MQ=255)            aCTGGAGATTTGTGACAGAATTATTGACCAATATGACTCGTGGACTGATATGTTTAAAGCACTACTGCATGAAACATTTAAAGTATATGGCGTTCTAttt                                                                        >  1:4366799/1‑100 (MQ=255)            aCTGGAGATTTGTGACAGAATTATTGACCAATATGACTCGTGGACTGATATGTTTAAAGCACTACTGCATGAAACATTTAAAGTATATGGCGTTCTAttt                                                                        >  1:1554468/1‑100 (MQ=255)            aCTGGAGATTTGTGACAGAATTATTGACCAATATGACTCGTGGACTGATATGTTTAAAGCACTACTGCATGAAACATTTAAAGTATATGGCGTTCTAttt                                                                        >  1:6549977/1‑100 (MQ=255)            aCTGGAGATTTGTGACAGAATTATTGACCAATATGACTCGTGGACTGATATGTTTAAAGCACTACTGCATGAAACATTTAAAGTATATGGCGTTCTAttt                                                                        >  1:3997788/1‑100 (MQ=255)            aCTGGAGATTTGTGACAGAATTATTGACCAATATGACTCGTGGACTGATATGTTTAAAGCACTACTGCATGAAACATTTAAAGTATATGGCGTTCTAttt                                                                        >  1:106585/1‑100 (MQ=255)            aCTGGAGATTTGTGACAGAATTATTGACCAATATGACTCGTGGACTGATATGTTTAAAGCACTACTGCATGAAACATTTAAAGTATATGGCGTTCTAttt                                                                        >  1:6244291/1‑100 (MQ=255)            aCTGGAGATTTGTGACAGAATTATTGACCAATATGACTCGTGGACTGATATGTTTAAAGCACTACTGCATGAAACATTTAAAGTATATGGCGTTCTAttt                                                                        >  1:6478421/1‑100 (MQ=255)            aCTGGAGATTTGTGACAGAATTATTGACCAATATGACTCGTGGACTGATATGTTTAAAGCACTACTGCATGAAACATTTAAAGTATATGGCGTTCTAttt                                                                        >  1:1382108/1‑100 (MQ=255)            aCTGGAGATTTGTGACAGAATTATCGACCAATATGACACGTGGACTGATTTGTTTAAAGCACTACTGCATGAAACATTTAAAGTATATGTTGTTCTAttt                                                                        >  1:6534475/1‑100 (MQ=255)            aCTGGAGATTTGTGACAGAATTATAGACCAATATGACTCGTGGACTGATATGTTTAAAGCACTACTGCATGAAACATTTAAAGTATATGGCGTTCTAttt                                                                        >  1:6618133/1‑100 (MQ=255)             ttGGAGATTTGTGACAGAATTATTGACCAATATGACTCGTGGACTGATATGTTTAAAGCACTACTGCATGAAACATTTAAAGTATATGGCgagatcggaa                                                                       >  1:1673081/2‑91 (MQ=255)                     ttGTGACAGAATTATTGACCAATATGACTCGTGGACTGATATGTTTAAAGCACTACTGCATGAAACATTTAAAGTATATGGCGTTCTATTTATAGATgcg                                                               >  1:4364640/1‑100 (MQ=255)                     ttGTGACAGAATTATTGACCAATATGACTCGTGGACTGATATGTTTAAAGCACTACTGCATGAAACATTTAAAGTATATGGCGTTCTATTTATAGATgcg                                                               >  1:6526991/1‑100 (MQ=255)                     ttGTGACAGAATTATTGACCAATATGACTCGTGGACTGATATGTTTAAAGCACTACTGCATGAAACATTTAAAGTATATGGCGTTCTATTTATAGATgcg                                                               >  1:486446/1‑100 (MQ=255)                     ttGTGACAGAATTATTGACCAATATGACTCGTGGACTGATATGTTTAAAGCACTACTGCATGAAACATTTAAAGTATATGGCGTTCTATTTATAGATgcg                                                               >  1:6517350/1‑100 (MQ=255)                      tgtgACAGAATTATTGACCAATATGACTCGTGGCCTGATATGTTTAAAGCACTACTGCCTGAAACATTTAACGTATATGGCGTTCTATTTATAGATgcgc                                                              >  1:754567/1‑100 (MQ=255)                      tgtgACAGAATTATTGACCAATATGACTCGTGGACTGATATGTTTAAAGCACTACTGCATGAAACATTTAAAGTATATGGCGTTCTATTTATAGATgcgc                                                              >  1:3036260/1‑100 (MQ=255)                       gtgACAGAATTATTGACCAATATGACTCGTGGACTGATATGTTTAAAGCACTACTGCATGAAACATTTAAAGTATATGGCGTTCTATTTATAGATGCGCa                                                             >  1:6078399/1‑100 (MQ=255)                         gACAGAATTATTGACCAATATGACTCGTGGACTGATATGTTTAAAGCACTACTGCATGAAACATTTAAAGTATATGGCGTTCTATTTATAGATGCGCAGt                                                           >  1:5955973/1‑100 (MQ=255)                         gACAGAATTATTGACCAATATGACTCGTGGACTGATATGTTTAAAGCACTACTGCATGAAACATTTAAAGTATATGGCGTTCTATTTATAGATACGCAga                                                           >  1:1463779/1‑99 (MQ=255)                          caacccctattGACCAATATGACTCGTGGACTGATATGTTTAAAGCACTACTGCATGAAACATTTAAAGTATATGGCGTTCTATTTATAGATGCGCAGtt                                                          >  1:4219009/8‑100 (MQ=255)                                ttattGACCAATATGACTCGTGGTCTGATATGTTTAAAGCACTACTGCATGAAACATTTAAAGTATATGGCGTTCTATTTATAGATGCGCAGTTTGAGcc                                                    <  1:5315037/100‑1 (MQ=255)                                  tctGACCAATATGACTCGTGGACTGATATGTTTAAAGCACTACTGCATGAAACATTTAAAGTATATGGCGTTCTATTTATAGATGCGCAGTTTGAGCCGt                                                  >  1:5027631/3‑100 (MQ=255)                                  attGACCAATATGACTCGTGGACTGATATGTTTAAAGCACTACTGCATGAAACATTTAAAGTATATGGCGTTCTATTTATAGATGCGCAGTTTGAGCCGt                                                  >  1:595744/1‑100 (MQ=255)                                  attGACCAATATGACTCGCGGACTGATATGTTTAAAGCACTACTGCATGAAACATTTAAAGCAAATGGCGTTCTATTTATAGATGCGCAGTTTGAGCCGt                                                  >  1:4012320/1‑100 (MQ=255)                                    tGACCAATATGACTCGTGGACTGATATGTTTAAAGCACTACTGCATGAAACATTTAAAGTATATGGCGTTCTATTTATAGATGCGCAGTTTGAGCCGTTa                                                >  1:1805430/1‑100 (MQ=255)                                    tGACCAATATGACTCGTGGACTGATATGTTTAAAGCACTACTGCATGAAACATTTAAAGTATATGGCGTTCTATTTATAGATGCGCAGTTTGAGCCGTTa                                                >  1:6443244/1‑100 (MQ=255)                                    tGACCAATATGACTCGTGGACTGATATGTTTAAAGCACTACTGCATGAAACATTTAAAGTATATGGCGTTCTATTTATAGATGCGCAGTTTGAGCCGTTa                                                >  1:3489217/1‑100 (MQ=255)                                    tGACCAATATGACTCGTGGACTGATATGTTTAAAGCACTACTGCATGAAACATTTAAAGTATATGGCGTTCTATTTATAGATGCGCAGTTTGAGCCGTTa                                                >  1:6645738/1‑100 (MQ=255)                                     gACCAATATGACTCGTGGACTGATATGTTTAAAGCACTACTGCATGAAACATTTAAAGTATATGGCGTTCTATTTATAGATGCGCAGTTTGAGCCGTTaa                                               >  1:6300569/1‑100 (MQ=255)                                      aCCAATATGACTCGTGGACTGATATGTTTAAAGCACTACTGCATGAAACATTTAAAGTATATGGCGTTCTATTTATAGATGCGCAGTTTGAGCCGTTAAg                                              >  1:1508568/1‑100 (MQ=255)                                          atatGACTCGTGGACTGATATGTTTAAAGCACTACTGCATGAAACATTTAAAGTATATGGCGTTCTATTTATAGATGCGCAGTTTGAGCCGTTAAGaaaa                                          >  1:2028042/1‑100 (MQ=255)                                            atGACTCGTGGACTGATATGTTTAAAGCACTACTGCATGAAACATTTAAAGTATATGGCGTTCTATTTATAGATGCGCAGTTTGAGCCGTTAAGAAAAAt                                        >  1:46000/1‑100 (MQ=255)                                            atGACTCGTGGACTGATATGTTTAAAGCACTACTGCATGAAACATTTAAAGTATATGGCGTTCTATTTATAGATGCGCAGTTTGAGCCGTTAAGAAAAAt                                        >  1:6283170/1‑100 (MQ=255)                                            atGACTCGTGGAATAATATGTTTACAGCACTACTACATGAAACATTTACAGAATTTGGCGTTCTATTTATAGATGCGCAGTTTGAGCTGTTATGAAAAAt                                        >  1:5814308/1‑100 (MQ=255)                                              gACTCGTGGACTGATATGTTTAAAGCACTACTGCATGAAACATTTAAAGTATATGGCGTTCTATTTATAGATGCGCAGTTTGAGCCGTTAAGatcggaag                                      >  1:1184135/1‑93 (MQ=255)                                              gACTCGTGGACTGATATGTTTAAAGCACTACTGCATGAAACATTTAAAGTATATGGCGTTCTATTTATAGATGCGCAGTTTGAGCCGTTAAGagaagatc                                      >  1:5097123/1‑96 (MQ=255)                                              gACTCGTGGACTGATATGTTTAAAGCACTACTGCATGAAACATTTAAAGTATATGGCGTTCTATTTATAGATGCGCAGTTTGAGCCGTTAAGaaagagat                                      >  1:838185/1‑95 (MQ=255)                                              gACTCGTGGACTGATATGTTTAAAGCACTACTGCATGAAACATTTAAAGTATATGGCGTTCTATTTATAGATGCGCAGTTTGAGCCGTTAAGAAAAATgg                                      >  1:138559/1‑100 (MQ=255)                                              gACTCGTGGACTGATATGTTTAAAGCACTACTGCATGAAACATTTAAAGTATATGGCGTTCTATTTATAGATGCGCAGTTTGAGCCGTTAAGAAAAATgg                                      >  1:4230571/1‑100 (MQ=255)                                              gACTCGTGGACTGATATGTTTAAAGCACTACTGCATGAAACATTTAAAGTATATGGCGTTCTATTTATAGATGCGCAGTTTGAGCCGTTAAGAAAAATgg                                      >  1:5455325/1‑100 (MQ=255)                                              gACTCGTGGACTGATATGTTTAAAGCACTACTGCATGAAACATTTAAAGTATATGGCGTTCTATTTATAGATGCGCAGTTTGAGCCGTTAAGAAAAATgg                                      >  1:2487756/1‑100 (MQ=255)                                              gACTCGTGGACTGATATGTTTAAAGCACTACTGCATGAAACATTTAAAGTATATGGCGTTCTATTTATAGATGCGCAGTTTGAGCCGTTAAGAAAAATgg                                      >  1:3748106/1‑100 (MQ=255)                                               aCTCGTGGACTGATATGTTTAAAGCACTACTGCATGAAACATTTAAAGTATATGGCGTTCTATTTATAGATGCGCAGTTTGAGCCGTTAAGGAAGAtcgg                                     >  1:3510114/1‑97 (MQ=255)                                                 tagTGGGCTGATATGTTTAAAGCACTACTGCATGAAACATTTAAAGTATATGGCGTTCTATTTATAGATGCGCAGTTTGAGCCGTTAAGAAAAATGGAAg                                   >  1:1070951/3‑100 (MQ=255)                                                  cGTGGACTGATATGTTTAAAGCACTACTGCATGAAACATTTAAAGTATATGGCGTTCTATTTATAGATGCGCAGTTTGAGCCGTTAAGAAGATCGGAAga                                  >  1:2175619/1‑99 (MQ=255)                                                  cGTGGACTGATATGTTTAAAGCACTACTGCATGAAACATTTAAAGTATATGGCGTTCTATTTATAGATGCGCAGTTTGAGCCGTTAAGAAAAATGGAAgc                                  >  1:1434104/1‑100 (MQ=255)                                                  cGTGGACTGATATGTTTAAAGCACTACTGCATGAAACATTTAAAGTATATGGCGTTCTATTTATAGATGCGCAGTTTGAGCCGTTAAGAAAAATGGAAgc                                  >  1:6345156/1‑100 (MQ=255)                                                   ctGGACTGATATGTTTAAAGCACTACTGCATGAAACATTTAAAGTATATGGCGTTCTATTTATAGATGCGCAGTTTGAGCCGTTAAGAAAAATGGAAgcg                                 >  1:5553680/2‑100 (MQ=255)                                                    tGGACTGATATGTTTAAAGCACTACTGCATGAAACATTTAAAGTATATGGCGTTCTCTTTATAGATGCGCAGTTTGAGCCGTTAAGAAAAATGGAAgcgc                                >  1:1991137/1‑100 (MQ=255)                                                                      aagtaactGCATGAAACATTTAAAGTATATGGCGTTCTATTTATAGATGCGCAGTTTGAGCCGTTAAGAAAAATGGAAGCGCCTATGTTTAAAAAGAttt              >  1:5096797/6‑100 (MQ=255)                                                                       cACTACTGCATGAAACATTTAAAGTATATGGCGTTCTATTTATAGATGCGCAGTTTGAGCCGTTAAGAAAAATGGAAGCGCCTATGTTTAAAAAGAtttt             >  1:6277433/1‑100 (MQ=255)                                                                        actactGCATGAAACATTTAAAGTATATGGCGTTCTATTTATAGATGCGCAGTTTGAGCCGTTAAGAAAAATGGAAGCGCCTATGTTTAAAAAGATTTTg            >  1:4772218/1‑100 (MQ=255)                                                                          ccctGCATGAAACATTTAAAGTATATGGCGTTCTATTTATAGATGCGCAGTTTGAGCCGTTAAGAAAAATGGAAGCGCCTATGTTTAAAAAGATTTTGaa          >  1:1834897/3‑100 (MQ=255)                                                                          cactGCATGAAACATTTAAAGTATATGGCGTTCTATTTATAGATGCGCAGTTTGAGCCGTTAAGAAAAATGGAAGCGCCTATGTTTAAAAAGATTTTGaa          >  1:5230027/2‑100 (MQ=255)                                                                           actGCATGAAACATTTAAAGTATATGGCGTTCTATTTATAGATGCGCAGTTTGAGCCGTTAAGAAAAATGGAAGCGCCTATGTTTAAAAAGATTTTGaaa         >  1:948601/1‑100 (MQ=255)                                                                           actGCATGAAACATTTAAAGTATATGGCGTTCTATTTATAGATGCGCAGTTTGAGCCGTTAAGAAAAATGGAAGCGCCTATGTTTAAAAAGATTTTGaaa         >  1:3983033/1‑100 (MQ=255)                                                                           actGCATGAAACATTTAAAGTATATGGCGTTCTATTTATAGATGCGCAGTTTGAGCCGTTAAGAAAAATGGAAGCGCCTATGTTTAAAAAGATTTTGaaa         >  1:2131378/1‑100 (MQ=255)                                                                           actGCATGAAACATTTAAAGTATATGGCGTTCTATTTATAGATGCGCAGTTTGAGCCGTTAAGAAAAATGGAAGCGCCTATGTTTAAAAAGATTTTGaaa         >  1:1444651/1‑100 (MQ=255)                                                                             tGCATGAAACATTTAAAGTATATGGCGTTCTATTTATAGATGCGCAGTTTGAGCCGTTAAGAAAAATGGAAGCGCCTATGTTTAAAAAGATTTTGaaaaa       >  1:1779907/1‑100 (MQ=255)                                                                              gCATGAAACATTTAAAGTATATGGCGTTCTATTTATAGATGCGCAGTTTGAGCCGTTAAGAAAAATGGAAGCGCCTATGTTGAAAAAGAtcggaaaagca      >  1:2760664/1‑90 (MQ=255)                                                                                  gAAACATTTAAAGTATATGGCGTTCTATTTATAGATGCGCAGTTTGAGCCGTTAAGAAAAATGGAAGCGCCTATGTTTAAAAAGATTTTGAAAAAACATc  >  1:3633317/1‑100 (MQ=255)                                                                                  aaaaCATTTAAAGTATATGGCGTTCTATTTATAGATGCGCAGTTTGAGCCGTTAAGAAAAATGGAAGCGCCTATGTTTAAAAAGATTTTGAAAAAACATc  >  1:4452559/2‑100 (MQ=255)                                                                                               |                                                                                       ACACAAGGTCTACTGGAGATTTGTGACAGAATTATTGACCAATATGACTCGTGGACTGATATGTTTAAAGCACTACTGCATGAAACATTTAAAGCATATGGCGTTCTATTTATAGATGCGCAGTTTGAGCCGTTAAGAAAAATGGAAGCGCCTATGTTTAAAAAGATTTTGAAAAAACATC  >  NC_007793/1170862‑1171042

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑