Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,168,149	A→G	intergenic (‑115/‑249)	SAUSA300_RS05785 ← / → SAUSA300_RS05790	tRNA‑Arg/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,168,149	0	A	G	100.0%	12.5 / NA	7	intergenic (‑115/‑249)	SAUSA300_RS05785/SAUSA300_RS05790	tRNA‑Arg/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (2/5);  total (2/5)

TAAATTAGCAATACCTTGTAAATAAAAAATGTTTATATTTTTCACTATTATAGAGCTATTTATCTAAAAAGGTTCAATAAGACTTAAATACGAATTCAGGCAACTTAATTGTGTTAAATACAGTTTTGAATGCCTAACTGTATTTCTTTTCTCTTTAAAATACAG  >  NC_007793/1168074‑1168238                                                                            |                                                                                          tAAATTAGCAATACCTTGTAAATAAAAAATGTTTATATTTTTCACTATTATAGAGCTATTTATCTAAAAAGGTTCGATAAGACTTAAATACGAATTCAgg                                                                   <  1:3999866/100‑1 (MQ=255)  aaaTTAGCAATACCTTGTAAATAAAAAATGTTTATATTTTTCACTATTATAGAGCTATTTATCTAAAAAGGTTCGATAAGACTTAAATACGAATTCAGGc                                                                  <  1:133924/100‑1 (MQ=255)     ttAGCAATACCTTGTAAATAAAAAATGTTTATATTTTTCACTATTATAGAGCTATTTATCTAAAAAGGTTCGATAAGACTTAAATACGAATTCAGGCaag                                                               <  1:4246612/100‑2 (MQ=255)     ttAGCAATACCTTGTAAATAAAAAATGTTTATATTTTTCACTATTATAGAGCTATTTATCTAAAAAGGTTCGATAAGACTTAAATACGAATTCAGGCAAc                                                               <  1:3144472/100‑1 (MQ=255)                ttGTAAATAAAAAATGTTTATATTTTTCACTATTATAGAGCTATTTATCTAAAAAGGTTCGATAAGACTTAAATACGAATTCAGGCAACTTAATTGTGtt                                                    <  1:278428/100‑1 (MQ=255)                                                                  aaaaaGGTTCGATAAGACTTAAATACGAATTCAGGCAACTTAATTGTGTTAAATACAGTTTTGAATGCCTAACTGTATTTCTTTTCTCTTTAAAATACAg  >  1:2316250/1‑100 (MQ=255)                                                                  aaaaaGGTTCGATAAGACTTAAATACGAATTCAGGCAACTTAATTGTGTTAAATACAGTTTTGAATGCCTAACTGTATTTCTTTTCTCTTTAAAATACAg  >  1:5470233/1‑100 (MQ=255)                                                                            |                                                                                          TAAATTAGCAATACCTTGTAAATAAAAAATGTTTATATTTTTCACTATTATAGAGCTATTTATCTAAAAAGGTTCAATAAGACTTAAATACGAATTCAGGCAACTTAATTGTGTTAAATACAGTTTTGAATGCCTAACTGTATTTCTTTTCTCTTTAAAATACAG  >  NC_007793/1168074‑1168238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑