Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,167,775	G→A	intergenic (+289/+186)	SAUSA300_RS05780 → / ← SAUSA300_RS05785	DNA‑binding protein/tRNA‑Arg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,167,775	0	G	A	100.0%	150.7 / NA	53	intergenic (+289/+186)	SAUSA300_RS05780/SAUSA300_RS05785	DNA‑binding protein/tRNA‑Arg
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/45);  total (8/45)

AATCTTGGTGTAGTGTTGGTGTAGTTCAGGTGCAGTATAGATGAAATTTGAGCACAAAATTACTTAATTAAAAATGTATAAACAAGTGTCAGACGCGTGTCAATTGTGTGTCAAAAAATTCTATAACGAATAAGCATGCTTGTTTTAATTACTGTATTTTCGGGGCTTATGCGGGGGACGTTTTGGTG  >  NC_007793/1167679‑1167866                                                                                                 |                                                                                            gaTCTTGGTGTAGTGTTGGTGTAGTTCAGGTGCAGTATAGATGAAATTTGAGCACAAAATTACTTAATTAAAAATGTATAAACAAGTGTCAGACGCatgc                                                                                          <  1:4924157/99‑2 (MQ=255)  aTCTTGGTTTAGTGTTGGTGTAGTTCAGGTGCAGTATAGATGAAATTTGAGCACAAAATTACTTAATTAAAAATGTATAAACAAGTGTCAGACGCATGTc                                                                                         <  1:3097800/100‑1 (MQ=255)  aTCTTGGTGTAGTGTTGGTGTAGTTCAGGTGCAGTATAGATGAAATTTGAGCACAAAATTACTTAATTAAAAATGTATAAACAAGTGTCAGACGCatgca                                                                                         <  1:3328772/100‑3 (MQ=255)  aTCTTGGTGTAGTGTTGGTGTAGTTCAGGTGCAGTATAGATGAAATTTGAGCACAAAATTACTTAATTAAAAATGTATAAACAAGTGTCAGACGCATGTc                                                                                         <  1:1481510/100‑1 (MQ=255)  aTCTTGGTGTAGTGTTGGTGTAGTTCAGGTGCAGTATAGATGAAATTTGAGCACAAAATTACTTAATTAAAAATGTATAAACAAGTGTCAGACGCATGTc                                                                                         <  1:5206333/100‑1 (MQ=255)   tCTTGGTGTAGTGTTGGTGTAGTTCAGGTGCAGTATAGATGAAATTTGAGCACAAAATTACTTAATTAAAAATGTATAAACAAGTGTCAGACGCATGTCa                                                                                        <  1:6021783/100‑1 (MQ=255)   tCTTGGTGTAGTGTTGGTGTAGTTCAGGTGCAGTATAGATGAAATTTAGGCACAAAATTACTTAATTAAAAATGTATAAACAAGTGTCAGACGCATGTCa                                                                                        <  1:5655220/100‑1 (MQ=255)      tGGTGTAGTGTTGGTGTAGTTCAGGTGCAGTATAGATGAAATTTGAGCACAAAATTACTTAATTAAAAATGTATAAACAAGTGTCAGACGCATGTCAAtt                                                                                     >  1:518941/1‑100 (MQ=255)      tGGTGTAGTGTTGGTGTAGTTCAGGTGCAGTATAGATGAAATTTGAGCACAAAATTACTTAATTAAAAATGTATAAACAAGTGTCAGACGCATGTCAAtt                                                                                     >  1:2637503/1‑100 (MQ=255)      tGGTGTAGTGTTGGTGTAGTTCAGGTGCAGTATAGATGAAATTTGAGCACAAAATTACTTAATTAAAAATGTATAAACAAGTGTCAGACGCATGTCAAtt                                                                                     >  1:4065810/1‑100 (MQ=255)      tGGTGTAGTGTTGGTGTAGTTCAGGTGCAGTATAGATGAAATTTGAGCACAAAATTACTTAATTAAAAATGTATAAACAAGTGTCAGACGCATGTCAAtt                                                                                     >  1:3657879/1‑100 (MQ=255)      tGGTGCAGTGTTGGTGTAGTTCAGGTGCAGTATAGATGAAATTTGAGCACAAAATTACTTAATTAAAAATGTATAAACAAGTGTCAGACGCATGTCAAtt                                                                                     >  1:2339048/1‑100 (MQ=255)           tAGTGTTGGTGTAGTTCAGGTGCAGTATAGATGAAATTTGAGCACAAAATTACTTAATTAAAAATGTATAAACAAGTGTCAGACGCATGTCAATtgtgtg                                                                                <  1:5522831/100‑1 (MQ=255)            aGTGTTGGTGTAGTTCAGGTGCAGTATAGATGAAATTTGAGCACAAAATTACTTAATTAAAAATGTATAAACAAGTGTCAGACGCATGTCAATtgtgtgg                                                                               <  1:622433/100‑2 (MQ=255)             gtgtTGGTGTAGTTCAGGTGCAGTATAGATGAAATTTGAGCACAAAATTACTTAATTAAAAATGTATAAACAAGTGTCAGACGCATGTCAATTGTGTGTc                                                                              <  1:5031134/100‑1 (MQ=255)             gtgtTGGTGTAGTTCAGGTGCAGTATAGATGAAATTTGAGCACAAAATTACTTAATTAAAAATGTATAAACAAGTGTCAGACGCATGTCAATTGTGTGTc                                                                              <  1:228652/100‑1 (MQ=255)              tgtTGGTGTAGTTCAGGTGCAGTATAGATGAAATTTGAGCACAAAATTACTTAATTAAAAATGTATAACCAAGTGTCAGACGCATGTCAATTGTGTGTCa                                                                             <  1:2793231/100‑1 (MQ=255)               gtTGGTGTAGTTCAGGTGCAGTATAGATGAAATTTGAGCACAAAATTACTTAATTAAAAATGTATAAACAAGTGTCAGACGCATGTCAATTGTGTGTCaa                                                                            <  1:3822010/100‑1 (MQ=255)               gtTGGTGTAGTTCAGGTGCAGTATAGATGAAATTTGAGCACAAAATTACTTAATTAAAAATGTATAAACAAGTGTCAGACGCATGTCAATTGTGTGTCaa                                                                            <  1:2930581/100‑1 (MQ=255)               gtTGGTGTAGTTCAGGTGCAGTATAGATGAAATTTGAGCACAAAATTACTTAATTAAAAATGTATAAACAAGTGTCAGACGCATGTCAATTGTGTGTCaa                                                                            <  1:3066190/100‑1 (MQ=255)                     ttAGTTCAGGTGCAGTATAGATGAAATTTGAGCACAAAATTACTTAATTAAAAATGTATAAACAAGTGTCAGGCGCATGTCAATTGTGTGTCAAAAAAtt                                                                      >  1:2759658/2‑100 (MQ=255)                       aGTTCAGGTGCAGTATAGATGAAATTTGAGCACAAAATTACTTAATTAAAAATGTATAAGCAAGTGTCAGACGCATGTCAATTGTGTGTCAAAAAATTCt                                                                    <  1:3399618/100‑1 (MQ=255)                         ttCAGGTGCAGTATAGATGAAATTTGAGCACAAAATTACTTAATTAAAAATGTATAAACAAGTGTCAGACGCATGTCAATTGTGTGTCAAAAAATTCtat                                                                  <  1:823665/100‑1 (MQ=255)                           cAGGTGCAGTATAGATGAAATTTGAGCACAAAATTACTTAATTAAAAATGTATAAACAAGTGTCAGACGCATGTCAATTGTGTGTCAAAAAATTCTATaa                                                                >  1:5446051/1‑100 (MQ=255)                              ttGCAGTATAGATGAAATTTGAGCACAAAATTACTTAATTAAAAATGTATAAACAAGTGTCAGACGCATGTCAATTGTGTGTCAAAAAATTCTATAACGa                                                             >  1:2081403/2‑100 (MQ=255)                                gCAGTATAGATGAAATTTGAGCACAAAATTACTTAATTAAAAATGTATAAACAAGTGTCAGACGCATGTCAATTGTGTGTCAAAAAATTCTATAACGaag                                                           <  1:6289485/100‑2 (MQ=255)                                        gATGAAATTTGAGCACAAAATTACTTAATTAAAAGTGTATAAACAAGTGTCAGACGCATGTCAATTGTGTGTCAAAAAATTCTATAACGAATAAGCagag                                                   <  1:1338346/100‑4 (MQ=255)                                          tGAAATTTGAGCACAAAATTACTTAATTAAAAATGTATAAACAAGTGTCAGACGCATGTCAATTGTGTGTCAAAAAATTCTATAACGAATAAGCATGCtt                                                 <  1:3841686/100‑1 (MQ=255)                                                       caAAATTACTTAATCAAAAATGTATAAACAAGTGTCAGACGCATGTCAATTGTGTGTCAAAAAATTCTATAACGAATAAGCATGCTTGTTTTAATTACTg                                    <  1:4935933/100‑1 (MQ=255)                                                          aaTTACTTAATTAAAAATGTATAAACAGGTGTCAGACGCATGTCAATTGTGTGTCAAAAAATTCTATAACGAATAAGCATGCTTGTTTTAATTACTGTaa                                 <  1:584433/100‑2 (MQ=255)                                                                  aattaaAAATGTATAAACAAGTGTCAGACGCATGTCAATTGTGTGTCAAAAAATTCTATAACGAATAAGCATGCTTGTTTTAATTACTGTATTTTCgggg                         <  1:1811394/100‑1 (MQ=255)                                                                   attaaAAATGTATAAACAAGTGTCAGACGCATGTCAATTGTGTGTCAAAAAATTCTATAACGAATAAGCATGCTTGTTTTAATTACTGTATTTTCGGGGc                        <  1:4255119/100‑1 (MQ=255)                                                                    ttaaAAATGTATAAACAAGTGTCAGACGCATGTCAATTGTGTGTCAAAAAATTCTATAACGAATAAGCATGCTTGTTTTAATTACTGTATTTTCGGGGCt                       <  1:3460612/100‑1 (MQ=255)                                                                    ttaaAAATGTATAAACAAGTGTCAGACGCATGTCAATTGTGTGTCAAAAAATTCTATAACGAATAAGCATGCTTGTTTTAATTACTGTATTTTCGGGGCt                       <  1:526559/100‑1 (MQ=255)                                                                    ttaaAAATGTATAAACAAGTGTCAGACGCATGTCAAGTGTGTGTCAAAAAATTCTATAACGAATAAGCATGCTTGTTTTAATTACTGTATTTTCGGGGcg                       <  1:6429632/100‑2 (MQ=255)                                                                     taaAAGTGTATAAGCAAGTGTCAGACGCATGTCAATTGTGTGTCAAAAAATTCTATAACGAATAAGCATGCTTGTTTTAATTACTGTATTTTCGGGGCtt                      <  1:3572554/100‑1 (MQ=255)                                                                     taaAAATGTATAATCAAGTGTCAGACGCATGTCAATTGTGTGTCAAAAAATTCTATAACGAATAAGCATGCTTGTTTTAATTACTGTATTTTCGGGGCtg                      <  1:527423/100‑2 (MQ=255)                                                                     taaAAATGTATAAACAAGTGTCAGACGCATGTCAATTGTGTGTCAAAGAATTCTATAACGAATAAGCATGCTTGTTTTAATTACTGTATTTTCGGGGCtt                      <  1:2673601/100‑1 (MQ=255)                                                                     taaAAATGTATAAACAAGTGTCAGACGCATGTCAATTGTGTGTCAAAAAATTCTATAACGAATAAGCATGCTTGTTTTAATTACTGTATTTTCGGGGCtt                      <  1:6340910/100‑1 (MQ=255)                                                                     taaAAATGTATAAACAAGTGTCAGACGCATGTCAATTGTGTGTCAAAAAATTCTATAACGAATAAGCATGCTTGTTTTAATTACTGTATTTTCGGGGCtt                      <  1:6386464/100‑1 (MQ=255)                                                                     taaAAATGTATAAACAAGTGTCAGACGCATGTCAATTGTGTGTCAAAAAATTCTATAACGAATAAGCATGCTTGTTTTAATTACTGTATTTTCGGGGCtt                      <  1:6071146/100‑1 (MQ=255)                                                                     taaAAATGTATAAACAAGTGTCAGACGCATGTCAATTGTGTGTCAAAAAATTCTATAACGAATAAGCATGCTTGTTTTAATTACTGTATTTTCGGGGCtt                      <  1:2743118/100‑1 (MQ=255)                                                                     taaAAATGTATAAACAAGTGTCAGACGCATGTCAATTGTGTGTCAAAAAATTCTATAACGAATAAGCATGCTTGTTTTAATTACTGTATTTGCGGGGCtt                      <  1:3972915/100‑1 (MQ=255)                                                                     taaAAATGTATAAACAAGTGTCAGACGCATGCCAATTGTGTGTCAAAAAATTCTATAACGAATAAGCATGCTTGTTTTAATTACTGTATTTTCGGGGCtt                      <  1:3865183/100‑1 (MQ=255)                                                                      aaaaaTGTATAAACAAGTGTCAGACGCATGTCAATTGTGTGTCAAAAAATTCTATAACGAATAAGCATGCTTGTTTTAATTACTGTATTTTCGGGGCttg                     <  1:3056986/100‑2 (MQ=255)                                                                       aaaaTGTATAAACAAGTGTCAGACGCATGTCAATTGTGTGTCAAAAAATTCTATAACGAATAAGCATGCTTGTTTTAATTACTGTATTTTCGGGGCTTac                    <  1:1148196/100‑2 (MQ=255)                                                                            gTATAAACAAGTGTCAGACGCATGTCAATTGTGTGTCAAAAAATTCTATAACGAATAAGCATGCTTGTTTTAATTACTGTATTTTCGGGGCTTATGCggg               <  1:200549/100‑1 (MQ=255)                                                                            gTATAAACAAGTGTCAGACGCATGTCAATTGTGTGTCAAAAAATTCTATAACGAATAAGCATGCTTGTTTTAATTACTGTATTTTCGGGGCTTATGCggg               <  1:1386518/100‑1 (MQ=255)                                                                             tataAACAAGTGTCAGACGCATGTCAATTGTGTGTCAAAAAGTTCTATAACGAATAAGCATGCTTGTTTTAATTACTGTATTTTCGGGGCTTATGCgggc              <  1:2444295/100‑2 (MQ=255)                                                                              ataAACAAGTGTCAGACGCATGTCAATTGTGTGTCAAAAAATTCTATAACGAATAAGCATGCTTGTTTTAATTACTGTATTTTCGGGGCTTATGCggggg             <  1:2720428/100‑1 (MQ=255)                                                                              ataAACAAGTGTCAGACGCATGTCAATTGTGTGTCAAAAAATTCTATAACGAATAAGCATGCTTGTTTTAATTACTGTATTTTCGGGGCTTATGCggggg             <  1:104394/100‑1 (MQ=255)                                                                              ataAACAAGTGTCAGACGCATGTCAATTGTGTGTCAAAAAATTCTATAACGAATAAGCATGCTTGTTTTAATTACTGTATTTTCGGGGCTTATGCgggat             <  1:6463707/100‑3 (MQ=255)                                                                                  aCAAGTGTCAGACGCATGTCAATTGTGTGTCAAAAAATTCTATAACGAATAAGCATGCTTGTTTTAATTACTGTATTTTCGGGGCTTATGCGGGGGACGt         <  1:3750833/100‑1 (MQ=255)                                                                                        gtCAGACGCATGTCAATTGTGTGTCAAAAAATTCTATAACGAATAAGCATGCTTGTTTTAATTACTGTATTTTCGGGGCTTATGCGGGGGACGTTTTggg   <  1:819759/100‑2 (MQ=255)                                                                                         tCAGACGCATGTCAATTGTGTGTCAAAAAATTCTATAACGAATAAGCATGCTTGTTTTAATTACTGTATTTTCGGGGCTTATGCGGGGGACGTTTTGGTg  <  1:3018706/100‑1 (MQ=255)                                                                                                 |                                                                                            AATCTTGGTGTAGTGTTGGTGTAGTTCAGGTGCAGTATAGATGAAATTTGAGCACAAAATTACTTAATTAAAAATGTATAAACAAGTGTCAGACGCGTGTCAATTGTGTGTCAAAAAATTCTATAACGAATAAGCATGCTTGTTTTAATTACTGTATTTTCGGGGCTTATGCGGGGGACGTTTTGGTG  >  NC_007793/1167679‑1167866

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑