Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,167,649	T→G	intergenic (+163/+312)	SAUSA300_RS05780 → / ← SAUSA300_RS05785	DNA‑binding protein/tRNA‑Arg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,167,649	0	T	G	100.0%	66.1 / NA	27	intergenic (+163/+312)	SAUSA300_RS05780/SAUSA300_RS05785	DNA‑binding protein/tRNA‑Arg
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (8/19);  total (8/19)

TATAATTATAGGATTTCGGGCCCCTGAAAAGTCCCTAAAATTTTGAATAGTATCAAAATCTATAGGAATAATATAATAATAAAAATCTCTACGCATGCAAATGGTGAGTGTTAAAAATCTTGGTGTAGTGTTGGTGTAGTTCAGGTGCAGTATAGATGAAATTTGAGCACAAAATTACTT  >  NC_007793/1167564‑1167743                                                                                      |                                                                                               ttaccgcataGGATTTCGGGCCCCTGAAAAGTCCCTAAAATTTTGAATAGTATCAAAATCTATAGGAATAATATAATAATAAAAAGCTCTACGCATGCaa                                                                                  <  1:2241197/93‑1 (MQ=255)      ctataGGATTTCGGGCCCCTGAAAAGTCCCTAAAATTTTGAATAGTATCAAAATCTATAGGAATAATATAATAATAAAAAGCTCTACGCATGCAAATGGt                                                                             >  1:2396477/2‑100 (MQ=255)        ataGGATTTCGGGCCCCTGAAAAGTCCCTAAAATTTTGAATAGTATCAAAATCTATAGGAATAATATAATAATAAAAAGCTCTACGCATGCAAATGGTGa                                                                           >  1:5379704/1‑100 (MQ=255)                  gtgtcccTGAAAAGTCCCTAAAATTTTGAATAGTATCAAAATCTATAGGAATAATATAATAATAAAAAGCTCTACGCATGCAAATGGTGAGTGTTaaaaa                                                                 >  1:5584373/5‑100 (MQ=255)                  gggCCCCTGAAAAGTCCCTAAAATTTTGAATAGTATCAAAATCTATAGGAATAATATAATAATAAAAAGCTCTACGCATGCAAATGGTGAGTGTTaaaaa                                                                 >  1:1096462/1‑100 (MQ=255)                   ggCCCCTGAAAAGTCCCTAAAATTTTGAATAGTATCAAAATCTATAGGAATAATATAATAATAAAAAGCTCTACGCATGCAAATGGTGAGTGTTAAAAAt                                                                >  1:2306409/1‑100 (MQ=255)                           aaaaGTCCCTAAAATTTTGAATAGTATCAAAATCTATAGGAATAATATAATAATAAAAAGCTCTACGCATGCAAATGGTGAGTGTTAAAAATCTTGgtgt                                                        <  1:1673162/100‑1 (MQ=255)                           aaaaGTCCCTAAAATTTTGAATAGTATCAAAATCTATAGGAATAATATAATAATAAAAAGCTCTACGCATGCAAATGGTGAGTGTTAAAAATCTTGgtgt                                                        <  1:1529065/100‑1 (MQ=255)                           aaaaGTCCCTAAAATTTTGAATAGTATCAAAATCTATAGGAATAATATAATAATAAAAAGCTCTACGCATGCAAATGGTGAGTGTTAAAAATCTTGgtgt                                                        <  1:6483151/100‑1 (MQ=255)                           aaaaGTCCCTAAAATTTTGAATAGTATCAAAATCTATAGGAATAATATAATAATAAAAAGCTCTACGCATGCAAATGGTGAGTGTTAAAAATCTTGgtgt                                                        <  1:2252887/100‑1 (MQ=255)                           aaaaGTCCCTAAAATTTTGAATAGTATCAAAATCTATAGGAATAATATAATAATAAAAAGCTCTACGCATGCAAATGGTGAGTGTTAAAAATCTTGgtgt                                                        <  1:3141520/100‑1 (MQ=255)                           aaaaGTCCCTAAAATTTTGAATAGTATCAAAATCTATAGGAATAATATAATAATAAAAAGCTCTACGCATGCAAATGGTGAGTGTTAAAAATCTTGgtgt                                                        <  1:3892269/100‑1 (MQ=255)                           aaaaGTCCCTAAAATTTTGAATAGTATCAAAATCTATAGGAATAATAAAATAATAAAAAGCTCTACGCATGCAAATGGTGAGTGTTAAAAATCTTGgtgt                                                        <  1:6088384/100‑1 (MQ=255)                            aaaGTCCCTAAAATTTTGAATAGTATCAAAATCTATAGGAATAATATAATAATAAAAAGCTCTACGCATGCAAATGGTGAGTGTTAAAAATCTTGGTGTa                                                       <  1:1387515/100‑1 (MQ=255)                                tCCCTAAAATTTTGAATAGTATCAAAATCTATAGGAATAATATAATAATAAAAAGCTCTACGCATGCAAATGGTGAGTGTTAAAAATCTTGGTGTAgtgt                                                   <  1:1061925/100‑1 (MQ=255)                                      aaaTATTGAATAGTATCAAAATCTATAGGAATAATATAATAATAAAAAGCTCTGCGCATGCAAATGGTGAGTGTGAAAAATCTTGGTGTAGTGTTGgtgt                                             <  1:3077846/100‑1 (MQ=255)                                           ttGAATAGTATCAAAATCTATAGGAATAATATAATAATAAAAAGCTCTACGCATGCAAATGGTGAGTGTTAAAAATCTTGGTGTAGTGTTGGTGTAGTTc                                        <  1:2458059/100‑1 (MQ=255)                                                caGTATCAAAATCTATAGGAATAATATAATAATAAAAAGCTCTACGCATGCAAATGGTGAGTGTTAAAAATCTTGGTGTAGTGTTGGTGTAGTTCAggcg                                   >  1:4398491/2‑98 (MQ=255)                                                      tccgaTCTATAGGGATAATATAATAATAAAAAGCTCTACGCATGCAAATGGTGAGTGTTAAAAATCTTGGTGTAGTGTTGGTGTAGTTCAGGTGCAGtat                             <  1:5795415/96‑1 (MQ=255)                                                      cAAAATCTATAGGAATAATATAGTAATAAAAAGCTCTACGCATGCAAATGGTGAGTGTTAAAAATCTTGGTGTAGTGTTGGTGTAGTTCAGGTGCAGtat                             <  1:6349668/100‑1 (MQ=255)                                                      cAAAATCTATAGGAATAATATAATAATAAAAAGCTCTACGCATGCAAATGGTGAGTGTTAAAAATCTTGGTGTAGTGTTGGTGTAGTTCAGGTGCAGtat                             <  1:3203957/100‑1 (MQ=255)                                                      cAAAATCTATAGGAATAATATAATAATAAAAAGCTCTACGCATGCAAATGGTGAGTGTTAAAAATCTTGGTGTAGTGTTGGTGTAGTTCAGGTGCAGtat                             <  1:5313519/100‑1 (MQ=255)                                                      cAAAATCTATAGGAATAATATAATAATAAAAAGCTCTACGCATGCAAATGGTGAGTGTTAAAAATCTTGGGGTAGTGTTGGTGTAGTTCAGGTGCAGtat                             <  1:1452182/100‑1 (MQ=255)                                                              ataGGAATAATATAATAATAAAAAGCTCTACGCATGCAAATGGCGAGTGTTAAAAATCTTGGTGTAGTGTTGGTGTAGTTCAGGTGTAGTAGAGAtcgga                     >  1:6597569/1‑96 (MQ=255)                                                              ataGGAATAATATAATAATAAAAAGCTCTACGCAGGCAAATGGTGAGTGTTAAAAATCTTGGAGTAGTGTTGGTGTAGTTCAGGTGCAGtcagatcggaa                     >  1:1972629/1‑90 (MQ=255)                                                                     taatataataATAAAAAGCTCTACGCAAGCAAATGGTGAGTGTTAAAAATCATGGTGTAGTGTTGGTGTAGTTCAGGTGCAGTATAGATGAAATTTGAGc              <  1:1742291/100‑1 (MQ=255)                                                                                 aaaaaGCTCTACGCATGCAAATGGTGAGTGTTAAAAATCTTGGTGTAGTGTTGGTGTAGTTCAGGTGCAGTATAGATGAAATTTGAGCACAAAATTACtt  <  1:1943510/100‑1 (MQ=255)                                                                                      |                                                                                               TATAATTATAGGATTTCGGGCCCCTGAAAAGTCCCTAAAATTTTGAATAGTATCAAAATCTATAGGAATAATATAATAATAAAAATCTCTACGCATGCAAATGGTGAGTGTTAAAAATCTTGGTGTAGTGTTGGTGTAGTTCAGGTGCAGTATAGATGAAATTTGAGCACAAAATTACTT  >  NC_007793/1167564‑1167743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑