Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_007793 141,817 G→A Q212Q (CAG→CAA)  SAUSA300_RS00635 → siderophore biosynthesis protein SbnF

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_007793141,8170GA99.6% 782.1 / ‑5.8 278Q212Q (CAG→CAA) SAUSA300_RS00635siderophore biosynthesis protein SbnF
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (267/10);  total (268/10)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.64e-01

Only 100 of 296 total aligned reads displayed.

AGATAAAGTAGAAACGACGGTATCAAGAAATGTTGTAGTTAACGAAATGTTACGTCAACAAGTTGGCGATAAGACTTATGAACATTTTGTACAGCAGATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGAACATGTCATCCAAGTTGATTTGGCTGAAGAA  >  NC_007793/141721‑141913
                                                                                                |                                                                                                
    ttcccaGAAACGACGGTATCAAGAAATGTTGTATTTAACGAAATGTTACGTCAACAAGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAg                                                                                           >  1:6026855/6‑100 (MQ=255)
    aaaGTAGAAACGACGGTATCAAGAAATGTTGTAGTTAACGAAATGTTACGTCAACAAGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAg                                                                                           >  1:218091/1‑100 (MQ=255)
    aaaGTAGAAACGACGGTATCAAGAAATGTTGTAGTTAACGAAATGTTACGTCAACAAGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAg                                                                                           >  1:5292082/1‑100 (MQ=255)
    aaaGTAGAAACGACGGTATCAAGAAATGTTGTAGTTAACGAAATGTTACGTCAACAAGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAg                                                                                           >  1:1601352/1‑100 (MQ=255)
       gTAGAAACGACGGTATCAAGAAATGTTGTAGTTAACGAAATGTTACGTCAACAAGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCAt                                                                                        >  1:3278777/1‑100 (MQ=255)
       gTAGAAACGACGGTATCAAGAAATGTTGTAGTTAACGAAATGTTACGTCAACAAGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCAt                                                                                        >  1:5769749/1‑100 (MQ=255)
       ctAGAAACGACGGTATCAAGAAATGTTGTAGTTAACGAAATGTTACGTCAACAAGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCAt                                                                                        >  1:1260221/2‑100 (MQ=255)
        caGAAACGACGGTATCAAGAAATGTTGTAGTTAACGAAATGTTACGTCAACAAGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATc                                                                                       >  1:608811/2‑100 (MQ=255)
         aGAAACGACGGTATCAAGAAATGTTGTAGTTAACGAAATGTTACGTCAACAAGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCt                                                                                      >  1:6117264/1‑100 (MQ=255)
         aGAAACGACGGTATCAAGAAATGTTGTAGTTAACGAAATGTTACGTCAACAAGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCt                                                                                      >  1:3726419/1‑100 (MQ=255)
         aGAAACGACGGTATCAAGAAATGTTGTAGTTAACGAAATGTTACGTCAACAAGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCt                                                                                      >  1:5578846/1‑100 (MQ=255)
         aGAAACGACGGTATCAAGAAATGTTGTAGTTAACGAAATGTTACGTCAACAAGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCt                                                                                      >  1:4745148/1‑100 (MQ=255)
          tcctcctcgGTACCAAGAAATGTTGTAGTTAACGAAATGTTACGTCAACAAGTTGGCGATAAGACTTATGAACATTTTGTACAGCCAATTGAAGCATCTg                                                                                     >  1:4914842/8‑100 (MQ=255)
           aaaCGACGGTATCAAGAAATGTTGTAGTTAACGAAATGTTACGTCAACAAGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTgg                                                                                    >  1:4165296/1‑100 (MQ=255)
            aaCGACGGTATCAAGAAATGTTGTAGTTAACGAAATGTTACGTCAACAAGTTGGCGATAAGGCTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGc                                                                                   >  1:4604074/1‑100 (MQ=255)
            aaCGACGGTATCAAGAAATGTTGTAGTTAACGAAATGTTACGTCAACAAGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGc                                                                                   >  1:4706540/1‑100 (MQ=255)
            aaCGACGGTATCAAGAAATGTTGTAGTTAACGAAATGTTACGTCAACAAGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGc                                                                                   >  1:6152954/1‑100 (MQ=255)
             acgacgGTATCAAGAAATGTTGTAGTTAACGAAATGTTACGTCAACAAGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCa                                                                                  >  1:1552256/1‑100 (MQ=255)
                gcgGTATCAAGAAATGTTGTAGTTAACGAAATGTTACGTCAACAAGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAAc                                                                               >  1:3975159/2‑100 (MQ=255)
                acgGTATCAAGAAATGTTGTAGTTAACGAAATGTTACGTCAACAAGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAAc                                                                               >  1:535192/1‑100 (MQ=255)
                acgGTATCAAGAAATGTTGTAGTTAACGAAATGTTACGTCAACAAGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAAc                                                                               >  1:3442697/1‑100 (MQ=255)
                 tggTATCAAGAAATGTTGTAGTTAACGAAATGTTACGTCAACAAGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACa                                                                              >  1:1392749/2‑100 (MQ=255)
                 cgGTATCAAGAAATATTGTAGTTAACGAAATGTTACGTCAACAAGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCaaata                                                                              <  1:4140150/100‑3 (MQ=255)
                     aTCAAGAAATGTTGTAGTTAACGAAATGTTACGTCAACAAGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTa                                                                          >  1:913144/1‑100 (MQ=255)
                     aTCAAGAAATGTTGTAGTTAACGAAATGTTACGTCAACAAGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTa                                                                          >  1:4590748/1‑100 (MQ=255)
                       cAAGAAATGTTGTAGTTAACGAAATGTTACGTCAACAAGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTaaa                                                                        >  1:1418155/1‑100 (MQ=255)
                             ctgttgtAGTTAACGAAATGTTACGTCAACAAGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGt                                                                  >  1:2572412/2‑100 (MQ=255)
                               gttgtAGTTAACGAAATGTTACGTCAACAAGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTg                                                                <  1:5626806/100‑1 (MQ=255)
                                 tgtAGTTAACGAAATGTTACGTCAACAAGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTgag                                                              >  1:3495424/1‑100 (MQ=255)
                                 tgtAGTTAACGAAATGTTACGTCAACAAGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTgag                                                              >  1:188299/1‑100 (MQ=255)
                                  gtAGTTAACGAAATGTTACGTCAACAAGTTGGCGATAAGACTTATGACCATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTgaga                                                             >  1:4080114/1‑100 (MQ=255)
                                  gtAGTTAACGAAATGTTACGTCAACAAGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTgaga                                                             >  1:169766/1‑100 (MQ=255)
                                  ccggTTAACGAAATGTTACGTCAACAAGTTGGCGATAAGACTTATGAACATTTTGCACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTgaga                                                             >  1:4267226/4‑100 (MQ=255)
                                       tAACGGAATGTTACGTCAACAAGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATa                                                        >  1:5017489/1‑100 (MQ=255)
                                                   aCGTCAACAAGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCa                                            >  1:3143622/1‑100 (MQ=255)
                                                       gaacaaGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGc                                        >  1:1867512/2‑100 (MQ=255)
                                                       caacaaGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTCAATGATGTTGAGATGATACCTGTACACCCATgga                                        >  1:5453051/1‑99 (MQ=255)
                                                       caacaaGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGc                                        >  1:3262381/1‑100 (MQ=255)
                                                       caacaaGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGc                                        >  1:5039332/1‑100 (MQ=255)
                                                         acaaGTTGGCGCTAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCaagatcg                                      >  1:5654995/1‑94 (MQ=255)
                                                          caaGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAga                                     >  1:146663/1‑99 (MQ=255)
                                                           aaGTTGGCGAGAAGACTTATGAACATTTTGCAAAGCAAATTGAAGCATCGGGCAAACATGCAAATGATGTCGAGATGATACCAGTATACCCATGGCAGtt                                    >  1:6623984/1‑100 (MQ=255)
                                                            tgatGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGttt                                   >  1:6603843/4‑100 (MQ=255)
                                                            tcttGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGttt                                   >  1:6488474/3‑100 (MQ=255)
                                                            ctctGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGCTGAGATGATACCTGTACACCCATGGCAGttt                                   >  1:4036503/4‑100 (MQ=255)
                                                            aGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTATATGATGTTGAGATGATACCTGTACACCCCTGGCAgatc                                   >  1:3879463/1‑97 (MQ=255)
                                                            aGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGttt                                   >  1:1004203/1‑100 (MQ=255)
                                                            aGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGttt                                   >  1:2506809/1‑100 (MQ=255)
                                                            aGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGCAAATGATGTTGAGATGATACCTGTACACCCATGGCAGttt                                   >  1:4992681/1‑100 (MQ=255)
                                                             gctGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTg                                  >  1:2826452/3‑100 (MQ=255)
                                                             cttGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTg                                  >  1:2461601/2‑100 (MQ=255)
                                                             cttGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTg                                  >  1:1033352/2‑100 (MQ=255)
                                                             attGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTg                                  >  1:796750/2‑100 (MQ=255)
                                                              ttGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGa                                 >  1:5811210/1‑100 (MQ=255)
                                                               tGGCGATAAGACTTATGAACATTTTGTACAGTAAATTGAAGCACCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGaa                                >  1:2767088/1‑100 (MQ=255)
                                                               tGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATGCCTGTACACCCATGGCAGTTTGaa                                >  1:1131367/1‑100 (MQ=255)
                                                               tGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATgggcagatcgg                                >  1:2865043/1‑91 (MQ=255)
                                                               tGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGaa                                >  1:5703153/1‑100 (MQ=255)
                                                               tGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGaa                                >  1:4846070/1‑100 (MQ=255)
                                                               tGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGaa                                >  1:2938436/1‑100 (MQ=255)
                                                               tGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGaa                                >  1:4639218/1‑100 (MQ=255)
                                                               tGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGaa                                >  1:2368031/1‑100 (MQ=255)
                                                               tGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGaa                                >  1:3782687/1‑100 (MQ=255)
                                                                ggCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGTAGTTTGAAc                               >  1:6374152/1‑100 (MQ=255)
                                                                ggCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGAAc                               >  1:2156427/1‑100 (MQ=255)
                                                                ggCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGAAc                               >  1:5938003/1‑100 (MQ=255)
                                                                ggCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGAAc                               >  1:4878248/1‑100 (MQ=255)
                                                                ggCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGAAc                               >  1:1997722/1‑100 (MQ=255)
                                                                ggCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGAAc                               >  1:6270218/1‑100 (MQ=255)
                                                                 gCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGAACa                              >  1:2239362/1‑100 (MQ=255)
                                                                     tAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGAACATGTc                          >  1:6402745/1‑100 (MQ=255)
                                                                     ctaGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGAACATGTc                          >  1:3407548/3‑100 (MQ=255)
                                                                      aaGACTTATGAACATTTTGTACAGCAGATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGAACATGTCa                         >  1:2657100/1‑100 (MQ=255)
                                                                      aaGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGAACATGTCa                         >  1:1497363/1‑100 (MQ=255)
                                                                      aaGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGAACATGTCa                         >  1:4401024/1‑100 (MQ=255)
                                                                      aaGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGAACATGTCa                         >  1:4460016/1‑100 (MQ=255)
                                                                         gcTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAACCATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGAACATGTCAccc                      >  1:6557945/2‑100 (MQ=255)
                                                                          cTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGAACATGTCATCCa                     >  1:1316058/1‑100 (MQ=255)
                                                                            tATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGAACATGTCATCCAAg                   >  1:2714079/1‑100 (MQ=255)
                                                                            tATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGAACATGTCATCCAAg                   >  1:1229072/1‑100 (MQ=255)
                                                                             acgAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGAACATGTCATCCAAGt                  >  1:5319680/3‑100 (MQ=255)
                                                                              tGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGAACATGTCATCCAAGtt                 >  1:3326227/1‑100 (MQ=255)
                                                                               gAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGAACATGTCATCCAAGTTg                >  1:652231/1‑100 (MQ=255)
                                                                               gAACATTTTGTACAGCAAATTGAAGCATATGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGAACATGTCATCCAAGTTg                >  1:6312604/1‑100 (MQ=255)
                                                                                        gTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGAACATGTCATCCAAGTTGATTCGGcga       <  1:5241124/100‑3 (MQ=255)
                                                                                         tACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGAACATGTCATCCAAGTTGATTTGGCTga      <  1:846454/100‑1 (MQ=255)
                                                                                         tACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGAACATGTCATCCAAGTTGATTTGGCTga      >  1:5282067/1‑100 (MQ=255)
                                                                                         gaCAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGAACATGTCATCCAAGTTGATTTGGCTga      >  1:3704837/2‑100 (MQ=255)
                                                                                         caCAGCAAATTGAAGCAGCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGAACATGTCATCCAAGTTGATTTGGCTga      >  1:6206039/2‑100 (MQ=255)
                                                                                         aaCAGCAAATTGAAGCATCTAGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGAACATGTCATCCAAGTTGATTTGGCTga      >  1:2090275/2‑100 (MQ=255)
                                                                                          aCAGCAAATTGAAGCATCTGGCAAACATGTACATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGAACATGTCATCCAAGTTGATTTGGcaaaa     >  1:3074522/1‑96 (MQ=255)
                                                                                          aCAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGAACATGTCATCCAAGTTGATTTGGCTgaa     >  1:1204093/1‑100 (MQ=255)
                                                                                          aCAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGAACATGTCATCCAAGTTGATTTGGCTgaa     >  1:6444766/1‑100 (MQ=255)
                                                                                           cggcaaATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGAACATGTCATCCAAGTTGATTTGGCTgaag    >  1:691541/3‑100 (MQ=255)
                                                                                           cagcaaATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGAACATGTCATCCAAGTTGATTTGGCTgaag    >  1:3220213/1‑100 (MQ=255)
                                                                                           cagcaaATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGAACATGTCATCCAAGTTGATTTGGCTgaag    >  1:3005794/1‑100 (MQ=255)
                                                                                           cagcaaATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGAACATGTCATCCAAGTTGATTTGGCTgaag    >  1:1578875/1‑100 (MQ=255)
                                                                                            ggcaaATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGAACATGTCATCCAAGTTGATTTGGCTgaaga   >  1:5512319/2‑100 (MQ=255)
                                                                                            agcaaATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGAACATGTCATCCAAGTTGATTTGGCTgaaga   >  1:5912982/1‑100 (MQ=255)
                                                                                             gcaaATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGAACATGTCATCCAAGTTGATTTGGCTgaagaa  >  1:3646035/1‑100 (MQ=255)
                                                                                                |                                                                                                
AGATAAAGTAGAAACGACGGTATCAAGAAATGTTGTAGTTAACGAAATGTTACGTCAACAAGTTGGCGATAAGACTTATGAACATTTTGTACAGCAGATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGAACATGTCATCCAAGTTGATTTGGCTGAAGAA  >  NC_007793/141721‑141913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑