Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,158,763	T→C	intergenic (‑124/+8)	SAUSA300_RS05735 ← / ← SAUSA300_RS05740	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,158,763	0	T	C	100.0%	26.4 / NA	12	intergenic (‑124/+8)	SAUSA300_RS05735/SAUSA300_RS05740	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (2/10);  total (2/10)

TACCTATTATATACATCATACACTTTAAAAATTATCATTTAAATAATCTGATTTGGAATTAAAGTGCATGAAGTATAAGTCACCTTCATATACTAATCAAAGAGGACGTCAACAGTTATTTTATTAGGATTTTTAACATAAACATTTGCTAGATCTGAATGTAAT  >  NC_007793/1158679‑1158843                                                                                     |                                                                                 tACCTATTATATACATCATACACTTTAAAAATTATCATTTAAATAATCTGATTTGGAATTAAAGTGCATGAAGTATAAGTCACCCTCATATACTAATCaa                                                                   <  1:804770/100‑1 (MQ=255)                           aaaaaGTATCATTTAAGTAATCTGATTTGGAATTAAAGTGCATGAAGTATAAGTCGCCCTCATATACTAATCAAAGAGGACGTCAACAGTTATTttatta                                         <  1:2015037/100‑1 (MQ=255)                                                  gATTTGGAATTAAAGTGCATGAAGTATAAGTCACCCTCATATACTAATCAAAGAGGACGTCAACAGTTATTTTATTAGGATTTTTAACATAAACATATGc                  >  1:2857298/1‑100 (MQ=255)                                                   aTTTGGAATTAAAGTGCATGACGTATAAGTAAACCTCATATACTAATCAAAGAGGACGTCAACAGTTATTTTATTAGGATTTTTAACATAAACATTTGCt                 <  1:4696047/100‑1 (MQ=255)                                                   aTTTGGAATTAAAGTGCATGAAGTATAAGTCACCCTCATATACTAGTCAAAGAGGACGTCAACAGTTATTTTATTAGGATTTTTAACATAAACATTTGCt                 <  1:6611425/100‑1 (MQ=255)                                                   aTTTGGAATTAAAGTGCATGAAGTATAAGTCACCCTCATATACTAATCAAAGAGGACGTCAACAGTTATTTTATTAGGATTTTTAACATAAACATTTGCt                 <  1:1292436/100‑1 (MQ=255)                                                   aTTTGGAATTAAAGTGCATGAAGTATAAGTCACCCTCATATACTAATCAAAGAGGACGTCAACAGTTATTTTATTAGGATTTTTAACATAAACATTTGCt                 <  1:2734543/100‑1 (MQ=255)                                                   aTTTGGAATTAAAGTGCATGAAGTATAAGTCACCCTCATATACTAATCAAAGAGGACGTCAACAGTTATTTTATTAGGATTTTTAACATAAACATTTGCt                 <  1:4385673/100‑1 (MQ=255)                                                   aTTTGGAATTAAAGTGCATGAAGTATAAGTCACCCTCATATACTAATCAAAGAGGACGTCAACAGTTATTTTATTAGGATTTTTAACATAAACATTTGCt                 <  1:5123662/100‑1 (MQ=255)                                                   aTTTGGAATTAAAGTGCATGAAGTATAAGTCACCCTCATATACTAATCAAAGAGGACGTCAACAGTTATTTTATTAGGATTTTTAACATAAACATTTGCt                 <  1:5735498/100‑1 (MQ=255)                                                   aTTTGGAATTAAAGTGCATGAAGTATAAGTCACCCTCATATACTAATCAAAGAGGACGTCAACAGTTATTTTATTAGGATTTTTAACATAAACATTTGCt                 <  1:95895/100‑1 (MQ=255)                                                                  gCATGAAGTATAAGTCACCCTCATATACTAATCAAAGAGGACGTCAACAGTTATTTTATTAGGATTTTTAACATAAACATTTGCTAGATCTGACTGTAAt  >  1:3475929/1‑100 (MQ=255)                                                                                     |                                                                                 TACCTATTATATACATCATACACTTTAAAAATTATCATTTAAATAATCTGATTTGGAATTAAAGTGCATGAAGTATAAGTCACCTTCATATACTAATCAAAGAGGACGTCAACAGTTATTTTATTAGGATTTTTAACATAAACATTTGCTAGATCTGAATGTAAT  >  NC_007793/1158679‑1158843

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑