Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,154,522	A→C	intergenic (+325/‑229)	SAUSA300_RS05695 → / → SAUSA300_RS05700	fibrinogen‑binding protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,154,522	0	A	C	100.0%	150.9 / NA	61	intergenic (+325/‑229)	SAUSA300_RS05695/SAUSA300_RS05700	fibrinogen‑binding protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (1/60);  total (1/60)

ACAATATTAAATTATCATTTCCAGTTCTAGTGCTATATTGGTAGTAGTTGACTAAATGAAAATAAGCTTATAACAAGTTTTTTCAATACTCGTGGGGCCACAACAGAGAGAAATAGGATCACCAATTCCAACAAACAATGCAAGTTGGCGGGGGCCCCAATATAGAAGCTGGCGAAAAGTCAGCTTACAAAAATGTGCA  >  NC_007793/1154423‑1154621                                                                                                    |                                                                                                    aCAATATTAAATTATCATTTCCAGTTCTAGTGCTATATTGGTAGTAGTTGACTAAATGAAAATAAGCTTATAACAAGTTTTTTCAATACTCGTGGGGCca                                                                                                     <  1:4450415/100‑1 (MQ=255)       tcttccgaTCTTTTCCAGTTCTAGTGCTATATTGGTAGTAGTTGACTAAATGAAAATAAGCTTATAACAAGTTTTTTCAATACTTGTGGGGCCCCAACag                                                                                               <  1:1153557/93‑1 (MQ=38)         tcttccgatcttCCAGTTCTAGTGCGATATTGGTAGTAGTTGACTAAATGAAAATAAGCTTATAACAAGTTTTGTCAATGCTCGTGGGGCCCCAACagag                                                                                             <  1:772968/90‑1 (MQ=37)                tctttCCAGTTCTAGTGCTATATTGGTAGTAGTTGACTAAATGAAAATAAGCTTATAACAAGTTTTTTCAATACTCGTGGGGCCCCAACAGAGAGAAATa                                                                                      <  1:2767766/98‑1 (MQ=255)                gctcttccgatCTAGTGCTATATTGGTAGTAGTTGACTAAATGAAAATAAGCTTATAACAAGTTTTTTCAATACTCGTGGGGCCCCAACAGAGAGAAATa                                                                                      <  1:5485539/90‑1 (MQ=38)                gctcttccgatCTAGTGCTATATTGGTAGTAGTTGACTAAATGAAAATAAGCTTATAACAAGTTTTTTCAATACTCGTGGGGCCCCAACAGAGAGAAATa                                                                                      <  1:222375/90‑1 (MQ=38)                gatcttccgatCTAGTGCTATATTGGTAGTAGTTGACTAAATGAAAATAAGCTTATAACAAGTTTTTTCAATACTCGTGGGGCCCCAACAGAGAGAAATa                                                                                      <  1:6139932/90‑1 (MQ=38)                atcttCCAGTTCTAGTGCTATATTGGTAGTAGTTGACTAAATGAAAATAAGCTTATAACAAGTTTTTTCAATACTCGTGGGGCCCCAACAGAGAGAAATa                                                                                      <  1:3840714/97‑1 (MQ=255)                  tttCCAGTTCTAGTGCTATATTGGTAGTAGTTGACTAAATGAAAATAAGCTTATAACAAGTTTTTTCAATACTCGTGGGGCCCCAACAGAGAGAAATAgg                                                                                    <  1:4995422/100‑1 (MQ=255)                     tccgatCTAGTGCTATATTGGTAGTAGTTGACTAAATGAAAATAAGCTTATAACAAGTTTTTTCAATACTCGTGGGGCCCCAACAGAGAGAAATAGGATc                                                                                 <  1:4039331/95‑1 (MQ=255)                     tccgatCTAGTGCTATATTGGTAGTAGTTGACTAAATGAAAATAAGCTTATAACAAGTTTTTTCAATACTCGTGGGGCCCCAACAGAGAGAAATAGGATc                                                                                 <  1:5241249/95‑1 (MQ=255)                     ccAGTTCTAGTGCTATATTGGTAGTAGTTGACTAAACGAAAATAAGCTTATAACAAGTTTTTTCAATACTCGTGGGGCCCCAACAGAGAGAAATAGGATc                                                                                 <  1:6124700/100‑1 (MQ=255)                      ccgatCTGGTGCTATATTGGTAGTAGTTGACTAAATGAAAATAAGCTTATAACAAGTTTTTTCAATACTCGTGGGGCCCCAACAGAGAGAAATAGGATCa                                                                                <  1:2083924/96‑1 (MQ=255)                       cgatCTAGTGCTATATTGGTAGTAGTCGACTAAATGAAAATAAGCTTATAACAAGTTTTTTCAATCCTCGTGGGGCCCCAACAGAGAGAAATAGAATCAc                                                                               <  1:2809877/97‑1 (MQ=255)                             aGTGCTATATTGGTAGTAGTTGACTAAATGAAAATAAGCTTATAACAAGTTTTTTCAATACTCGTGGGGCCCCAACAGAGAGAAATAGGATCACCAAttt                                                                         >  1:4794128/1‑99 (MQ=255)                               tGCTATATTGGTAGTAGTTGACTAAATGAAAATAAGCTTATAACAAGTTTTTTCAATACTCGTGGGGCCCCAACAGAGAGAAATAGTATCACCAATTTca                                                                       <  1:4160993/100‑1 (MQ=255)                               tGCTATATTGGTAGTAGTTGACTAAATGAAAATAAGCTTATAACAAGTTTTTTCAATACTCGTGGGGCCCCAACAGAGAGAAATAGGATCACCAAtgata                                                                       <  1:5611250/100‑5 (MQ=255)                                 cTATATTGGTAGTAGTTGACTAAATGAAAATAAGCTTATAACAAGTTTTTTCAATACTCGTGGGGCCCCAACAGAGAGAAATAGGATCACCAATTTcaac                                                                     <  1:3761427/100‑1 (MQ=255)                                 cTATATTGGTAGTAGTTGACTAAATGAAAATAAGCTTATAACAAGTTTTTTCAATACTCGTGGGGCCCCAACAGAGAGAAATAGGATCACCAATTTcaac                                                                     <  1:5535714/100‑1 (MQ=255)                                   atatTGGTAGTAGTTGACTAAATGAAAATAAGCTTATAACAAGTTTTTTCAATACTCGTGGGGCCCCAACAGAGAGAAATAGGATCACCAATTTcaacag                                                                   <  1:2528931/100‑2 (MQ=255)                                     atTGGTAGTAGTTGACTAAATGAAAATAAGCTTATAACAAGTTTTTTCAATACTCGTGGGGCCCCAACAGAGAGAAATAGGATCACCAATTTCaacagac                                                                 <  1:1807045/100‑1 (MQ=255)                                      ttGGTAGTAGTTGACTAAATGAAAATAAGCTTATAACAAGTTTTTTCAATACTCGTGGGGCCCCAACAGAGAGAAATAGGATCACCAATTTCaacagaca                                                                <  1:311622/100‑1 (MQ=255)                                      ctGGTAGTAGTTGACTAAATGAAAATAAGCTTATAACAAGTTTTTCCAATACTCGTGGGGCCCCAACAGAGAGAAATAGGATCACCAATTTCaacagaca                                                                <  1:6093474/99‑1 (MQ=255)                                         gtagtaGTTGACTAAATGAAAATAAGCTTATAACAAGTTTTTTCAATACTCGTGGGGCCCCAACAGAGAGAAATAGGATCACCAATTTCAACAGACAATg                                                             <  1:440526/100‑1 (MQ=255)                                          tagtagTTGACTAAATGAAAATAAGCTTATAACAAGTTTTTTCAATACTCGTGGGGCCCCAACAGAGAGAAATAGGATCACCAATTTCAACAGACAATGc                                                            <  1:3057318/100‑1 (MQ=255)                                          tagtagTTGACTAAATGAAAATAAGCTTATAACAAGTTTTTTCAATACTCGTGGGGCCCCAACAGAGAGAAATAGGATCACCAATTTCAACAGACAATGc                                                            <  1:1842671/100‑1 (MQ=255)                                          tagtagTTGACTAAATGAAAATAAGCTTATAACAAGTTTTTTCAATACTCGTGGGGCCCCAACAGAGAGAAATAGGATCACCAATTTCAACAGACAATGc                                                            <  1:6099647/100‑1 (MQ=255)                                           tgtagtTGACTAAATGAAAATAAGCTTATAACAAGTTTTTTCAATACTCGTGGGGCCCCAACAGAGAGAAATAGGATCACCAATTTCAACAGACAATGCa                                                           <  1:3997374/99‑1 (MQ=255)                                           ctagtttGACTAAATGAAAATAAGCTTATAACAAGTTTTTTCAATACTCGTGGGGCCCCAACAGAGAGAAATAGGATCACCAATTTCAACAGACAATGCa                                                           <  1:2695421/95‑1 (MQ=255)                                           agtagtTGACTAAATGAAAATAAGCTTATAACAAGTTTTTTCAATGCTCGTGGGGCCCCAACAGAGAGAAATAGGATCACCAATTTCAACAGACAATGCa                                                           <  1:4246744/100‑1 (MQ=255)                                           agtagtTGACTAAATGAAAATAAGCTTATAACAAGTTTTTTCAATACTCGTGGGGCCCCAACAGAGAGAAATAGGATCACCAATTTCAACAGACAATGCa                                                           <  1:40730/100‑1 (MQ=255)                                           agtagtTGACTAAATGAAAATAAGCTTATAACAAGTTTTTTCAATACTCGTGGGGCCCCAACAGAGAGAAATAGGATCACCAATTTCAACAGACAATGCa                                                           <  1:5116645/100‑1 (MQ=255)                                           agtagtTGACTAAATGAAAATAAGCTTATAACAAGTTTTTTCAATACTCGTGGGGCCCCAACAGAGAGAAATAGGATCACCAATTTCAACAGACAATGCa                                                           <  1:5621919/100‑1 (MQ=255)                                           agtagtTGACTAAATGAAAATAAGCTTATAACAAGTTTTTTCAATACTCGTGGGGCCCCAACAGAGAGAAATAGGATCACCAATTTCAACAGACAATGCa                                                           <  1:6467936/100‑1 (MQ=255)                                           agtagtTGACTAAATGAAAATAAGCTTATAACAAGTTTTTTCAATACTCGTGGGGCCCCAACAGAGAGAAATAGGATCACCAATTTCAACAGACAATGCa                                                           <  1:3690928/100‑1 (MQ=255)                                           agtagtTGACTAAATGAAAATAAGCTTATAACAAGTTTTTTCAATACTCGTGGGGCCCCAACAGAGAGAAATAGGATCACCAATTTCAACAGACAATGCa                                                           <  1:2508687/100‑1 (MQ=255)                                           agtagtTGACTAAATGAAAATAAGCTTATAACAAGTTTTTTCAATACTCGTGGGGCCCCAACAGAGAGAAATAGGATCACCAATTTCAACAGACAATGCa                                                           <  1:2625313/100‑1 (MQ=255)                                           agtagtTGACTAAATGAAAATAAGCTTATAACAAGTTTTTTCAATACTCGTGGGGCCCCAACAGAGAGAAATAGGATCACCAATTTCAACAGACAATGCa                                                           <  1:2935350/100‑1 (MQ=255)                                           agtagtTGACTAAATGAAAATAAGCTTATAACAAGTTTTTTCAATACTCGTGGGGCCCCAACAGAGAGAAATAGGATCACCAATTTCAACAGACAATGCa                                                           <  1:4178294/100‑1 (MQ=255)                                            gtagtTGACTAAATGAAAATAAGCTTATAACAAGTTTTTTCAATACTCGTGGGGCCCCAACAGAGAGAAATAGGATCACCAATTTCAACAGACAATGCaa                                                          <  1:4611164/100‑1 (MQ=255)                                             tagtTGACTAAATGAAAATAAGCTTATAACAAGTTTTTTCAATACTCGTGGGGCCCCAACAGAGAGAAATAGGATCACCAATTTCAACAGACAATGCAAg                                                         <  1:6018914/100‑1 (MQ=255)                                             tagtTGACTAAATGAAAATAAGCTTATAACAAGTTTTTTCAATACTCGTGGGGCCCCAACAGAGAGAAATAGGATCACCAATTTCAACAGACAATGCAAg                                                         <  1:5961408/100‑1 (MQ=255)                                              agtTGACTAAATGAAAATAAGCTTATAACAAGTTTTTTCAATACTCGTGGGGCCCCAACAGAGAGAAATAGGATCACCAATTTCAACAGACAATGCAAGt                                                        <  1:1488702/100‑1 (MQ=255)                                               gtTGACTAAATGAAAATAAGCTTATAACAAGTTTTTTCAATACTCGTGGGGCCCCAACAGAGAGAAATAGGATCACCAATTTCAACAGACAATGCAAGtt                                                       <  1:1331525/100‑1 (MQ=255)                                                     tAAATGAAAATAAGCTTATAACAAGTTTTTTCAATACTCGTGGGGCCCCAACAGAGAGAAATAGGATCACCAATCTCAACAGACAATGCAAGTTggggtg                                                 <  1:6382030/100‑5 (MQ=21)                                                                   cTTATAACAAGTTTTTTCAATACTCGTGGGGCCCCAACAGAGAGAAATAGGATCACCAATTTCAACAGACAATGCAAGTTGGGGTGGGGCCCAACACAGa                                   <  1:4503484/100‑1 (MQ=18)                                                                   cTTATAACAAGTTTTTTCAATACTCGTGGGGCCCCAACAGAGAGAAATAGGATCACCAATTTCAACAGACAATGCAAGTTGGGGTGGGGCCCAACACAGa                                   <  1:5887995/100‑1 (MQ=18)                                                                         aCAAGTTTTTTCAATACTCGTGGGGCCCCAACAGAGAGAAATAGGATCCCCAATTTCAACAGACAATGCAAGTTGGGGTGGGGCCCAACACAGAAGCTgg                             <  1:1460394/100‑1 (MQ=17)                                                                         aCAAGTTTTTTCAATACTCGTGGGGCCCCAACAGAGAGAAATAGGATCACCAATTTCAACAGACAATGCAAGTTGGGGTGGGGCCCAACACAGAAGCTgg                             <  1:3076906/100‑1 (MQ=18)                                                                         aCAAGTTTTTTCAATACTCGTGGGGCCCCAACAGAGAGAAATAGGATCACCAATTTCAACAGACAATGCAAGTTGGGGTGGGGCCCAACACAGAAGCTgg                             <  1:3655574/100‑1 (MQ=18)                                                                         aCAAGTTTTTTCAATACTCGTGGGGCCCCAACAGAGAGAAATAGGATCACCAATTTCAACAGACAATGCAAGTTGGGGTGGGGCCCAACACAGAAGCTgg                             <  1:4743138/100‑1 (MQ=18)                                                                          cAAGTTTTTTCAATACTCGTGGGGCCCCAACAGAGAGAAATAGGATCACCAATTTCAACAGACAATGCAAGTTGGGGTGGGGCCCAACACAGAAGCTgag                            <  1:1363140/100‑3 (MQ=18)                                                                          cAAGTTTTTTCAATACTCGTGGGGCCCCAACAGAGAGAAATAGGATCACCAATTTCAACAGACAATGCAAGTTGGGGTGGGGCCCAACACAGAAGCTGGc                            <  1:4813823/100‑1 (MQ=18)                                                                          cAAGTTTTTTCAATACTCGTGGGGCCCCAACAGAGAGAAATAGGATCACCAATTTCAACAGACAATGCAAGTTGGGGTGGGGACCAACACAGAAGCTgga                            <  1:2948949/100‑2 (MQ=17)                                                                           aaGTTTTTTCAATACTCGTGGGGCCCCAACAGAGAGAAATAGGATCACCAATTTCAACAGACAATGCGAGTTGGGGTGGGGCCCAACACAGAAGCTGGcc                           <  1:1750006/100‑2 (MQ=18)                                                                           aaGTTTTTTCAATACTCGTGGGGCCCCAACAGAGAGAAATAGGATCACCAATTTCAACAGACAATGCAAGTTGGGGTGGGGCCCAACACAGAAGCTGGct                           <  1:4480504/100‑2 (MQ=18)                                                                           aaGTTTTTTCAATACTCGTGGGGCCCCAACAGAGAGAAATAGGATCACCAATTTCAACAGACAATGCAAGTTGGGGTGGGGCCCAACACAGAAGCTGGcc                           <  1:611775/100‑2 (MQ=18)                                                                           aaGTTTTTTCAATACTCGTGGGGCCCCAACAGAGAGAAATAGGATCACCAATTTCAACAGACAATGCAAGTTGGGGTGGGGCCCAACACAGAAGCTGGCg                           <  1:3479156/100‑1 (MQ=18)                                                                            aGTTTTTTCAATACTCGTGGGGCCCCAACAGAGAGAAATAGGATCACCAATTTCAACAGACAATGCAAGTTGGGGTGGGGCCCAACACAGAAGCTgggca                          <  1:340828/100‑4 (MQ=17)                                                                               tttttCAATACTCGTGGGGCCCCAACAGAGAGAAATAGGATCACCAATTTCAACAGACAATTCAAGTTGGGGTGGGGCCCAACACAGAAGCTGGCggtta                       <  1:3369648/100‑5 (MQ=18)                                                                               tttttCAATACTCGCGGGGCCCCAACAGAGAGAAATAGGATCACCAATTTCAACAGACAATGCAAGTTGGGGTGGGGCCCAACACAGAAGCTGGCgggta                       <  1:4994358/100‑5 (MQ=17)                                                                                  ttCAATACTCGTGGGGCCCCAACAGAGAGAAATAGGATCACCAATTTCAACAGACAATGCAAGTTGGGGTGGGGCCCAACACAGAAGCTGGCGAAAAGTc                    <  1:3769597/100‑1 (MQ=17)                                                                                                    acaacaGAGAGAAATAGGATCACCAATTTCAACAGACAATGCAAGTTGGGGTGGGGCCCAACACAGAAGCTGGCGAAAAGTCAGCATACAAAAATGTGCa  >  1:5112859/1‑100 (MQ=14)                                                                                                    |                                                                                                    ACAATATTAAATTATCATTTCCAGTTCTAGTGCTATATTGGTAGTAGTTGACTAAATGAAAATAAGCTTATAACAAGTTTTTTCAATACTCGTGGGGCCACAACAGAGAGAAATAGGATCACCAATTCCAACAAACAATGCAAGTTGGCGGGGGCCCCAATATAGAAGCTGGCGAAAAGTCAGCTTACAAAAATGTGCA  >  NC_007793/1154423‑1154621

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑