Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,143,651	T→C	S322P (TCT→CCT)	SAUSA300_RS05630 →	excinuclease ABC subunit C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,143,651	0	T	C	100.0%	39.8 / NA	17	S322P (TCT→CCT)	SAUSA300_RS05630	excinuclease ABC subunit C
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/0);  total (17/0)

AAACCAACATATTTTACCTAAGGAAGTTCATGTACCACGTAATTTGGATAAAGAAATGATTCAATCTGTTGTGGACACTAAAATCGTTCAACCCGCGCGAGGTCCCAAAAAAGATATGGTTGACCTAGCTGCACATAACGCTAAAGTATCCTTAAATAATA  >  NC_007793/1143587‑1143747                                                                 |                                                                                                 aaaCCAACATATTTTACCTAAGGAAGTTCATGTACCACGTAATTTGGATAAAGAAATGATTCAACCTGTTGTGGACACTAAAATCGTTCAACCCGCGCGa                                                               >  1:2237051/1‑100 (MQ=255)      aaCCTATTTTACCTAAGGAAGTTCATGTACCACGTAATTTGGATAAAGAAATGATTCAACCTGTTGTGGACACTAAAATCGTTCAACCcgctcgtgatcg                                                          >  1:5689278/1‑94 (MQ=255)               tACCTAAGGAAGTTCATGTACCACGTAATTTGGATAAAGAAATGATTCAACCTGTTGTGGACACTAAAATCGTTCAACCCGCGCGAGATcggaagagcac                                                 >  1:3135024/1‑90 (MQ=255)                aCCTAAGGAAGTTCATGTACCACGTAATTTGGATAAAGAAATGATTCAACCTGTTGTGGACACTAAAATCGTTCAACCCGCGCGAGGTCCCAAAAAAGat                                                >  1:1232643/1‑100 (MQ=255)                aCCTAAGGAAGTTCATGTACCACGTAATTTGGATAAAGAAATGATTCAACCTGTTGTGGACACTAAAATCGTTCAACCCGCGCGAGGTCCCAAAAAAGat                                                >  1:6083671/1‑100 (MQ=255)                   aaaGGAAGTTCATGTACCACGTAATTTGGATAAAGAAATGATTCAACCTGTTGTGGACACTAAAATCGTTCAACCCGCGCGAGGTCCCAAAAAAGATATg                                             >  1:5315390/2‑100 (MQ=255)                              aggTACCACGTAATTTGGATAAAGAAATGATTCAACCTGTTGTGGACACTAAAATCGTTCAACCCGCGCGAGGTCCCAAAAAAGATATGGTTGACCTAGc                                  >  1:5642360/3‑100 (MQ=255)                                gTACCACGTAATTTGGATAAAGAAATGATTCAACCTGTTGTGGACACTAAAATCGTTCAACCCGCGCGAGGTCCCAAAAAAGATATGGTTGACCTAGCTg                                >  1:3492720/1‑100 (MQ=255)                                gTACCACGTAATTTGGATAAAGAAATGATTCAACCTGTTGTGGACACTAAAATCGTTCAACCCGCGCGAGGTCCCAAAAAAGATATGGTTGACCTAGCTg                                >  1:4737573/1‑100 (MQ=255)                                gTACCACGTAATTTGGATAAAGAAATGATTCAACCTGTTGTGGACACTAAAATCGTTCAACCCGCGCGAGGTCCCAAAAAAGATATGGATGACCTAGCTg                                >  1:1974751/1‑100 (MQ=255)                                gTACCACGTAATTTGGATAAAGAAATGATTCAACCTGTTGTGGACACTAAAATCGTTCAACCCGCGCGAGGCCCCAAAAAAGATATGGTTGACCTAGCTg                                >  1:6601713/1‑100 (MQ=255)                                 ctccACGTAATTTGGATAAAGAAATGATTCAACCTGTTGTGGACACTAAAATCGTTCAACCCGCGCGAGGTCCCAAAAAAGATATGGTTGACCTAGCTGc                               >  1:6598834/3‑100 (MQ=255)                                     aCGTAATTTGGATAAAGAAATGATTCAACCTGTTGTGGACACTAAAATCGTTCAACCCGCGCGAGGTCCCAAAAAAGATATGGTTGACCTAGCTGCACAt                           >  1:5986517/1‑100 (MQ=255)                                                          gATTCAACCTGTTGTGGACACTAAAATCGTTCAACCCGCGCGAGGTCCCAAAAAAGATATGGTTGACCTAGCTGCACATAACGCTAAAGTATCCTTAaat      >  1:5099564/1‑100 (MQ=255)                                                            ctCAACCTGTTGTGGACACTAAAATCGTTCAACCCGCGCGAGGTCCCAAAAAAGATATGGTTGACCTAGCTGCACATAACGCTAAAGTATCCTTAaataa    >  1:1022731/2‑100 (MQ=255)                                                              cAACCTGTTGTGGACACTAAAATCGTTCAACCCGCGCGAGGTCCCAAAAAAGATATGGTTGACCTAGCTGCACATAACGCTAAAGTATCCTTAAataata  >  1:1420909/1‑100 (MQ=255)                                                              cAACCTGTTGTGGACACTAAAATCGTTCAACCCGCGCGAGGTCCCAAAAAAGACATGGTTGACCTAGCTGCACATAACGCTAAAGTATCCTTAAataata  >  1:5933331/1‑100 (MQ=255)                                                                 |                                                                                                 AAACCAACATATTTTACCTAAGGAAGTTCATGTACCACGTAATTTGGATAAAGAAATGATTCAATCTGTTGTGGACACTAAAATCGTTCAACCCGCGCGAGGTCCCAAAAAAGATATGGTTGACCTAGCTGCACATAACGCTAAAGTATCCTTAAATAATA  >  NC_007793/1143587‑1143747

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑