Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,136,229	A→G	A116A (GCT→GCC)	SAUSA300_RS05595 ←	ribonuclease HIII

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,136,229	0	A	G	100.0%	102.7 / NA	41	A116A (GCT→GCC)	SAUSA300_RS05595	ribonuclease HIII
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (5/36);  total (5/36)

CAGCAAGTTCTACAATTTTGGTATCGGTTAATTTTTTTGAATCATCTACACCCAAAGTTTTTAAAATAGGGACATGCTCTTTGGTAACGAAAGCAGCACACACAGTCAACGGACCAAAGTAATCGCCACTTCCAGCCTCATCACTACCAATACAGTTAAATTGATCATACATTAAAGTTTGTTCA  >  NC_007793/1136135‑1136319                                                                                               |                                                                                           cAGCAAGTTCTACAATTTTGGTATCGGTTAATTATTTTGAATCATCTACGCCCATAGTTTTTAATATAGGGACATGCTCTTTGGTAACGAAAGCGGcaca                                                                                       >  1:617958/1‑100 (MQ=255)         tCTACAATTTTGGTATCGGTTAATTTTTTTGAATCATCTACACCCAAAGTTTTTAAAATAGGGACATGCTCTTTGGTAACGAAAGCGGCACACACAGTCa                                                                               <  1:4585902/100‑1 (MQ=255)            aCAATTTTGGTATCGGTTAATTTTTTTGAATCATCTACACCCAAAGTTTTTAAAATAGGGACATGCTCTTTGGTAACGAAAGCGGCACACACAGTCAACg                                                                            >  1:3142111/1‑100 (MQ=255)            aCAATTTTGGTATCGGTTAA‑TTTTTTGAATCATCTACACCCAAAGTTTTTAAAATAGGGACATGCTCTTTGGTAACGAAAGCGGCACACACAGTCAACgg                                                                           <  1:1043785/100‑1 (MQ=255)                                  tgctcttccgatcTCCACCCAAAGTTTTTAAAATAGGGACATGCTCTTTGGTAACGAAAGCGGCACACACAGTCAACGGACCAAAGTAATCGCCACTTcc                                                      <  1:3111179/90‑1 (MQ=255)                                      cttccgatcTACACCCAAAGTTTTTAAAATAGGGACATGCTCTTTGGTAACGAAAGCGGCACACACAGTCAACGGACCAAAGTAATCGCCACTTCCAGcc                                                  <  1:5137617/94‑1 (MQ=255)                                        aaTCATCTACACCCAAAGTTTTTAAAATAGGGACATGCTCTTTGGTAACGAAAGCGGCACACACAGTCAACGGACCAAAGTAATCGCCACTTCCAGCCtg                                                <  1:683355/100‑2 (MQ=255)                                         ccgatcTACACCCAAAGTTTTTAAAATAGGGACATGCTCTTTGGTAACGAAAGCGGCACACACAGTCAACGGACCAAAGTAATCGCCACTTCCAGCCtca                                               <  1:5994243/97‑1 (MQ=255)                                         ccgatcTACACCCAAAGTTTTTAAAATAGGGACATGCTCTTTGGTAACGAAAGCGGCACACACAGTCAACGGACCAAAGTAATCGCCACTTCCAGCCtca                                               <  1:4930649/97‑1 (MQ=255)                                          cgatcTATTCCCAAAGTTTTTAAAATAGGGACGTGCTCTTTGGTAACGAAAGCGGCACACACAGTCAACGGACCAAAGTAATCGCCACTGCCAGCCtcat                                              <  1:4540778/98‑1 (MQ=255)                                          cgatcTACACCGAAAGTTTTTAAAATAGGGACATGCTCTTTGGTAACGAAAGCGGCACACACAGTCAACGGACCAAAGTAATCGCCACTTCCAGCCtcag                                              <  1:2395327/98‑2 (MQ=255)                                          cgatcTACACCCAAAGTTTTTAAAATAGGGACATGCTCTTTGGTAACGAAAGCGGCACACACAGTCAACGGACCAAAGTAATCGCCACTTCCAGCCtcag                                              <  1:600344/98‑2 (MQ=255)                                          cgatcTACACCCAAAGTGTTTAAAATAGGGACACGCTCTTTGGTAACGAAAGCGGCACACACAGGCAACGGACCAAAGTAATCGCCACTTCCAGCCtcag                                              <  1:5755225/98‑2 (MQ=255)                                           gatcTACACCCAAAGTTTTTAAAATAGGGACATGCTCTTTGGTAACGAAAGCGGCACACACAGTCAACGGACCAAAGTAATCGCCACTTCCAGCCtcact                                             <  1:1915664/99‑3 (MQ=255)                                           catcTACACCCAAAGTTTTTAAAATAGGGACATGCTCTTTGGTAACGAAAGCGGCACACACAGTCAACGGACCAAAGTAATCGCCACTTCCAGCCtcatc                                             <  1:3782258/100‑1 (MQ=255)                                             tcTACGCCCAAAGGTTTTAAAATAGGGACATGCTCTTTGGTAACGAAAGCGGCACACACAGTCAACGGACCAAAGTAATCGCCACTTCCAGCCTCATCAc                                           <  1:6130897/100‑1 (MQ=255)                                              gatctccccAAGTTTTTAAAATAGGGACATGCTCTTTGGTAACGAAAGCGGCACACACAGTCAACGGTCCAAAGTAATCGCCACTTCCAGCCTCATCACt                                          <  1:3478060/95‑1 (MQ=255)                                              cTACACCCAAAGTTTTTAAAATAGGGACATGCTCTTTGGTAACGAAAGCGGCACACACAGTCAACGGACCAAAGTAATCGCCACTTCCAGCCTCATCACt                                          <  1:3053824/100‑1 (MQ=255)                                              cTACACCCAAAGTTTTTAAAATAGGGACATGCTCTTTGGTAACGAAAGCGGCACACACAGTCAACGGACCAAAGTAATCGCCACTTCCAGCCTCATCACt                                          <  1:6420235/100‑1 (MQ=255)                                              cTACACCCAAAGTTTTTAAAATAGGGACATGCTCTTTGGTAACGAAAGCGGCACACACAGTCAACGGACCAAAGTAATCGCCACTTCCAGCCTCATCACt                                          <  1:2020846/100‑1 (MQ=255)                                                acgcccAAAGTTTTTAAAATAGGGACATGCTCTTTGGTAACGAAAGCGGCACACACAGTCAACGGACCAAAGTAATCGCCACTTCCAGCCTCATCACTAc                                        >  1:1868333/4‑100 (MQ=255)                                                acacCCAAAGTTTTTAAAATAGGGACATGCTCTTTGGTAACGAAAGCGGCACACACAGTCAACGGACCAAAGTAATCGCCACTTCCAGCCTCATCACTAc                                        >  1:2307381/1‑100 (MQ=255)                                                  gcccAAAGTTTTTAAAATAGGGACATGCTCTTTGGTAACGAAAGCGGCACACACAGTCAACGGACCAAAGTAATCGCCACTTCCAGCCTCATCACTACCa                                      <  1:5856419/99‑1 (MQ=255)                                                  acCCAAAGTTTTTAAAATAGGGACATGCTCTTTGGTAACGAAAGCGGCACACACAGTCAACGGACCAAAGTAATCGCCACTTCCAGCCTCATCACTACCa                                      <  1:1102648/100‑1 (MQ=255)                                                  acCCAAAGTTTTTAAAATAGGGACATGCTCTTTGGTAACGAAAGCGGCACACACAGTCAACGGACCAAAGTAATCGCCACTTCCAGCCTCATCACTACCa                                      <  1:3104436/100‑1 (MQ=255)                                                    ccAAAGTTTTTAAAGTAGGGACATGCTCTTTGGTAACGAAAGCGGCACACACAGTCAACGGACCAAAGTAATCGCCACTTCCAGCCTCATCACTACCAAt                                    <  1:4388318/100‑1 (MQ=255)                                                    ccAAAGTTTTTAAAATAGGGACATGCTCTTTGGTAACGAAAGCGGCACACACAGTCAACGGACCAAAGTAATCGCCACTTCCAGCCTCATCACTACCAAt                                    >  1:2660101/1‑100 (MQ=255)                                                     cAAAGTTTTTAAAATAGGGACATGCTCTTTGGTAACGAAAGCGGCACACACAGTCAACGGACCAAAGTAATCGCCACTTCCAGCCTCATCACTACCaagt                                   <  1:2815820/100‑3 (MQ=255)                                                      aaaGTTTTTAAAATAGGGACATGCTCTTTGGTAATGAAAGCGGCACACACAGTCAACGGACCAAAGTAATCGCCACTTCCAGCCTCATCACTACCAATAc                                  <  1:3820478/100‑1 (MQ=255)                                                      aaaGTTTTTAAAATAGGGACATGCTCTTTGGTAACGAAAGCGGCACACACAGTCAACGGACCAAAGTAATCGCCACTTCCAGCCTCATCACTACCAATAc                                  <  1:3836407/100‑1 (MQ=255)                                                      aaaGTTTTTAAAATAGGGACATGCTCTTTGGTAACGAAAGCGGCACACACAGTCAACGGACCAAAGTAATCGCCACTTCCAGCCTCATCACTACCAATAc                                  <  1:3657267/100‑1 (MQ=255)                                                      aaaGTGTTTAAAATAGGGACATGCTCTTTGGTAACGAAAGCGGCACACACAGTCAACGGACCAAAGTAATCGCCACTTCCAGCCTCATCACTACCAATAc                                  <  1:6610990/100‑1 (MQ=255)                                                       aaGTTTTTAAAATAGGGACATGCTCTTTGGTAACGAAAGCGGCACACACAGTCAACGGACCAAAGTAATCGCCACTTCCAGCCTCATCACTACCAATACa                                 <  1:2926035/100‑1 (MQ=255)                                                          tttttAAAATAGGGACATGCTCTTTGGTAACGAAAGCGGCACACACAGTCAACGGACCAAAGTAATCGCCACTTCCAGCCTCATCACTACCAATACAGtt                              <  1:2673535/100‑1 (MQ=255)                                                           ttttAAAATAGGGACATGCTCTTTGGTGACGAAAGCGGCACACACAGTCAACGGACCAAAGTAATCGCCGCTTCCAGCCTCATCACTACCAATACAGTTa                             <  1:2034695/100‑1 (MQ=255)                                                           ttttAAAATAGGGACATGCTCTTTGGTAACGAAAGCGGCACACACAGTCGACGGACCAAAGTAATCGCCACTTCCAGCCTCATCACTACCAATACAGTTa                             <  1:1206653/100‑1 (MQ=255)                                                           ttttAAAATAGGGACATGCTCTTTGGTAACGAAAGCGGCACACACAGTCAACGGACCAAAGTAATCGCCACTTCCAGCCTCATCACTACCAATACAGTTa                             <  1:3818135/100‑1 (MQ=255)                                                            tttAAAATAGGGACATGCTCTTTGGTAACGAAAGCGGCACACACAGTCAACGGACCAAAGTAATCGCCACTTCCAGCCTCATCACTACCAATACAGTTaa                            <  1:1325400/100‑1 (MQ=255)                                                                   tAGGGACATGCTCTTTGGTAACGAAAGCGGCACACACAGTCAACGGACCAAAGTAATCGCCACTTCCAGCCTCATCACTACCAATACAGTTAAATTGATc                     <  1:4179253/100‑1 (MQ=255)                                                                                   ggTAACGAAAGCGGCACACACAGTCAACGGACCAAAGTAATCGCCACTTCCAGCCTCATCACTACCAATACAGTTAAATTGATCATACATTAAAGTTTGt     <  1:414038/100‑1 (MQ=255)                                                                                      aaCGAAAGCGGCACACACAGTCAACGGACCAAAGTAATCGCCACTTCCAGCCTCATCACTACCAATACAGTTAAATTGATCATACATTAAAGTTTGTTCa  <  1:6265887/100‑1 (MQ=255)                                                                                               |                                                                                           CAGCAAGTTCTACAATTTTGGTATCGGTTAATTTTTTTGAATCATCTACACCCAAAGTTTTTAAAATAGGGACATGCTCTTTGGTAACGAAAGCAGCACACACAGTCAACGGACCAAAGTAATCGCCACTTCCAGCCTCATCACTACCAATACAGTTAAATTGATCATACATTAAAGTTTGTTCA  >  NC_007793/1136135‑1136319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑