Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,116,591	T→G	G330G (GGT→GGG)	SAUSA300_RS05480 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,116,591	0	T	G	100.0%	119.6 / NA	49	G330G (GGT→GGG)	SAUSA300_RS05480	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (27/22);  total (27/22)

GTTATGCGTTTAATGATGTACCAACATTAATCCATAGTTGGATGAATTCAGATATACATCGATCTCGTCTATTAAATTCAAAATACGATGAGATGGGTGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAGGGGTATTTATGATTAATGAAGCTTCACTAGCGATATTAGAT  >  NC_007793/1116494‑1116687                                                                                                  |                                                                                                 gTTATGCGTTTAATGATGTACCAACATTAATCCATAGTTGGATGAATTCAGATATACATCGATCTCGTCTATTAAATTCAAAATACGATGAGATgggggg                                                                                                <  1:88347/100‑1 (MQ=255) gTTATGCGTTTAATGATGTACCAACATTAATCCATAGTTGGATGAATTCAGATATACATCGATCTCGTCTATTAAATTCAAAATACGATGAGATCGgggg                                                                                                <  1:981870/100‑1 (MQ=255)        gccgaaTGATGTACCAACATTAATCCATAGTTGGATGAATTCAGATATACATCGATCTCGTCTATTAAATTCAAAATACGATGAGATGGGGGGAGATGTa                                                                                         >  1:3155017/5‑100 (MQ=255)                 tgTACCAACATTAATCCATAGTTGGATGAATTCAGATATACATCGATCTCGTCTATTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGAGat                                                                                >  1:579882/1‑100 (MQ=255)                 ggTACCAACATTAATCCATAGTTGGATGAATTCAGATATACATCGATCTCGTCTATTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGAGat                                                                                >  1:4765688/2‑100 (MQ=255)                             aaTCCATAGTTGGATGAATTCAGATATACATCGATCTCGTCTATTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTa                                                                    >  1:1499745/1‑100 (MQ=255)                                ccATAGTTGGATGAATTCAGATATACATCGATCTCGTCTATTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAAtt                                                                 >  1:5754757/1‑100 (MQ=255)                                agctAGTTGGATGAATTCAGATATACATCGATCTCGTCTATTAAATTCAACATACGATGAGATGGGGGGAGATGTACTGAGAGATTATTATTCACTAAtt                                                                 >  1:6535018/4‑100 (MQ=255)                                    aGTTGGATGAATTCAGATATACATCGATCTCGTCTATTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCt                                                             >  1:2178032/1‑100 (MQ=255)                                    aGTTGGATGAATTCAGATATACATCGATCTCGTCTATTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCt                                                             >  1:243566/1‑100 (MQ=255)                                    aGTTGGATGAATTCAGATATACATCGATCTCGTCTATTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCt                                                             >  1:2522614/1‑100 (MQ=255)                                    aGTTGGATGAATTCAGATATACATCGATCTCGTCTATTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCt                                                             >  1:2916209/1‑100 (MQ=255)                                        ggATGAATTCAGATATACATCGATCTCGTCTATTAAATTCAAAATACGTTGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGa                                                         >  1:645067/1‑100 (MQ=255)                                           tGAATTCAGATATACATCGATCTCGTCTATTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGaaaa                                                      >  1:1810873/1‑100 (MQ=255)                                                    tgctcttccGATCTCGTCTGTTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATg                                             <  1:5615315/92‑1 (MQ=255)                                                    atatACATCGATCTCGTCTATTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAGTg                                             >  1:4777096/1‑100 (MQ=255)                                                     tataCATCGATCTCGTCTATTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGa                                            >  1:1075596/1‑100 (MQ=255)                                                      ctcttccGATCTCGTCTATTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGATATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAg                                           <  1:1218066/94‑1 (MQ=255)                                                      ataCATCGATCTCGTCTATTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGaa                                           >  1:147435/1‑99 (MQ=255)                                                       tcttccGATCTCGTCTATTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAaggagg                                          <  1:5777028/95‑1 (MQ=255)                                                       tcttccGATCTCGTCTATTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAGTGAgg                                          <  1:2384110/95‑1 (MQ=255)                                                       tcttccGATCTCGTCTATTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAgg                                          <  1:4279952/95‑1 (MQ=255)                                                       tcttccGATCTCGTCTATTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAgg                                          <  1:849805/95‑1 (MQ=255)                                                       tcttccGATCTCGTCTATTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATAAgg                                          <  1:3094652/95‑1 (MQ=255)                                                       tcttccGATCTCGCCTATTAAATTCAAAAGACGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAACAATAACAACGAgg                                          <  1:4784979/95‑1 (MQ=255)                                                        cttccGATCTCGTCTATTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAACGAggg                                         <  1:182270/96‑1 (MQ=255)                                                        cttccGATCTCGGCTATTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAggc                                         <  1:3687542/96‑2 (MQ=255)                                                        cttccGATCACGTCTGTTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAggg                                         <  1:5310317/96‑1 (MQ=255)                                                         ttccGATCTCGTCTATTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGGGATTATTATTCCCTAATTTTCTTAGAAAAATAACAATGAgggg                                        <  1:1658582/97‑1 (MQ=255)                                                         ttccGATCTCGTCTATTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAgggg                                        <  1:2551425/97‑1 (MQ=255)                                                         ttccGATCTCGGCTATTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAgggt                                        <  1:3650896/97‑2 (MQ=255)                                                          tccGATCTCGTCTGTTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAgggag                                       <  1:1893493/98‑3 (MQ=255)                                                          tccGATCTCGTCTATTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAggggc                                       <  1:4128194/98‑2 (MQ=255)                                                          tccGATCTCGTCTATTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAGGGGt                                       <  1:4216356/98‑1 (MQ=255)                                                          tccGATCACGTCTATTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAGGGGt                                       <  1:738445/98‑1 (MQ=255)                                                           ccGATCTCGTCTATTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAGGGGtt                                      <  1:458125/99‑2 (MQ=255)                                                           ccGATCTCGTCTATTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAGGGGTa                                      <  1:4198120/99‑1 (MQ=255)                                                            cGATCTCGTCTATTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAGGGGTag                                     <  1:926391/100‑2 (MQ=255)                                                                ctcGTCTATTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAGGGGTagatcg                                 >  1:107607/1‑95 (MQ=255)                                                                  tgTCTATTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAggggacagatcgg                               >  1:6484983/2‑91 (MQ=255)                                                                  cGTCTATTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAACTAACAATAAggggagatcggca                               >  1:4802791/1‑91 (MQ=255)                                                                  cGTCTATTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAgggaagatcggaa                               >  1:3325225/1‑90 (MQ=255)                                                                  cGTCTATTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAGGGGTaagatcgg                               >  1:4023464/1‑93 (MQ=255)                                                                  cGTCTATTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAG‑AAAATAACAATGAggggagatcggaag                              >  1:3222030/1‑90 (MQ=255)                                                                  cGTCTATTAAATTCAAAATACGATGAGATGGGGGGAGATGCAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAACGAgggagatcggaag                               >  1:3146346/1‑90 (MQ=255)                                                                     cTATTAAATTCAAAATACGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAGGGGTATTTATGATTa                            <  1:173618/100‑1 (MQ=255)                                                                                     aCGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAGGGGTATTTATGATTAATGAAGCTTCACTAGc            >  1:5637180/1‑100 (MQ=255)                                                                                     aCGATGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAGGGGTATTTATGATTAATGAAGCTTCACTAGc            >  1:4576926/1‑100 (MQ=255)                                                                                       actGAGATGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAGGGGTATTTATGATTAATGAAGCTTCACTAGCGa          >  1:815951/3‑100 (MQ=255)                                                                                         agcaagggggggAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAGGGGTATTTATGATTAATGAAGCTTCACTAGCGata        >  1:3980434/7‑100 (MQ=255)                                                                                           tcctGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAGGGGTATTTATGATTAATGAAGCTTCACTAGCGATAtt      >  1:5062052/4‑100 (MQ=255)                                                                                           agaTGGGGGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAGGGGTATTTATGATTAATGAAGCTTCACTAGCGATAtt      >  1:4884951/1‑100 (MQ=255)                                                                                               ggggggAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAGGGGTATTTATGATTAATGAAGCTTCACTAGCGATATTAgat  >  1:1726706/1‑100 (MQ=255)                                                                                                  |                                                                                                 GTTATGCGTTTAATGATGTACCAACATTAATCCATAGTTGGATGAATTCAGATATACATCGATCTCGTCTATTAAATTCAAAATACGATGAGATGGGTGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAACAATGAGGGGTATTTATGATTAATGAAGCTTCACTAGCGATATTAGAT  >  NC_007793/1116494‑1116687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑