Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_007793 1,101,328 T→A S77S (TCA→TCT)  SAUSA300_RS05410 ← divalent metal cation transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0077931,101,3280TA86.2% 1300.3 / 147.4 543S77S (TCA→TCT) SAUSA300_RS05410divalent metal cation transporter
Reads supporting (aligned to +/- strand):  ref base T (21/54);  new base A (3/465);  total (24/519)
Fisher's exact test for biased strand distribution p-value = 8.73e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.55e-04

Only 100 of 544 total aligned reads displayed.

TAGGTCTTGATAAATAATGTCGTGTCATTTGAGCTAAGTCCATACCTGTTGCTATTCCTAATCTCACTGTCATGCTTTGAAGTAACATTGCTGATAAACTTGAAATAAGAATTACGAATAGCAAAGTATAGCCATATTGGGCGCCACCTTGCATTGATGTTATCCAGTTTCCGGGATCCATGTAACCAAC  >  NC_007793/1101237‑1101426
                                                                                           |                                                                                                  
caggcttatttaaaaaaTGACGTGTCATTTGTGCTAAATCCATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCt                                                                                            <  1:1660205/90‑1 (MQ=255)
caggcttatttaaaaaaTGACGTGTCATTTGTGCTAAATCCATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCt                                                                                            <  1:4369157/90‑1 (MQ=255)
caggcttatttaaaaaaTGACGTGTCATTTGTGCTAAATCCATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCt                                                                                            <  1:2265853/90‑1 (MQ=255)
caggcttatttaaaaaaTGACGTGTCATTTGTGCTAAATCCATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCt                                                                                            <  1:26552/90‑1 (MQ=255)
caggcttatttaaaaaaTGACGTGTCATTTGTGCTAAATCCATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCt                                                                                            <  1:4248730/90‑1 (MQ=255)
caggcttatttaaaaaaTGACGTGTCATTTGTGCTAAATCCATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCt                                                                                            <  1:4667878/90‑1 (MQ=255)
caggcttatttaaaaaaTGACGTGTCATTTGTGCTAAATCCATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCt                                                                                            <  1:1801970/90‑1 (MQ=255)
caggcttatttaaaaaaTGACGTGTCATTTGTGCTAAATCCATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCt                                                                                            <  1:4222963/90‑1 (MQ=255)
caggcttatttaaaaaaTGACGTGTCATTTGTGCTAAATCCATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCt                                                                                            <  1:5226366/90‑1 (MQ=255)
caggcttatttaaaaaaTGACGTGTCATTTGTGCTAAATCCATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCt                                                                                            <  1:4098020/90‑1 (MQ=255)
caggcttatttaaaaaaTGACGTGTCATTTGTGCTAAATCCATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCt                                                                                            <  1:1183446/90‑1 (MQ=255)
caggcttatttaaaaaaTGACGTGTCATTTGTGCTAAATCCATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCt                                                                                            <  1:3833604/90‑1 (MQ=255)
  ggcttatttaaaaaaTGACGTGTCATTTGTGCTAAATCCATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCtag                                                                                          <  1:314333/92‑3 (MQ=255)
  ggcttatttaaaaaaTGACGTGTCATTTGTGCTAAATCCATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCtag                                                                                          <  1:4494569/92‑3 (MQ=255)
  ggcttatttaaaaaaTGACGTGTCATTTGTGCTAAATCCATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCtag                                                                                          <  1:3296052/92‑3 (MQ=255)
  ggcttatttaaaaaaTGACGTGTCATTTGTGCTAAATCCATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCtag                                                                                          <  1:5655233/92‑3 (MQ=255)
  ggcttatttaaaaaaTGACGTGTCATTTGTGCTAAATCCATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCtag                                                                                          <  1:3957797/92‑3 (MQ=255)
  ggcttatttaaaaaaTGACGTGTCATTTGTGCTAAATCCATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCtag                                                                                          >  1:297687/9‑98 (MQ=255)
  ggcttatttaaaaaaTGACGTGTCATTTGTGCTAAATCCATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCtag                                                                                          <  1:2937949/92‑3 (MQ=255)
  ggcttatttaaaaaaTGACGTGTCATTTGTGCTAAATCCATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCtag                                                                                          <  1:5895614/92‑3 (MQ=255)
  ggcttatttaaaaaaTGACGTGTCATTTGTGCTAAATCCATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCtag                                                                                          <  1:2766919/92‑3 (MQ=255)
  ggcttatttaaaaaaTGACGTGTCATTTGTGCTAAATCCATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCtag                                                                                          <  1:3599086/92‑3 (MQ=255)
  ggcttatttaaaaaaTGACGTGTCATTTGTGCTAAATCCATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCtag                                                                                          <  1:3622381/92‑3 (MQ=255)
  ggcttatttaaaaaaTGACGTGTCATTTGTGCTAAATCCATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCtag                                                                                          <  1:4573560/92‑3 (MQ=255)
  ggcttatttaaaaaaTGACGTGTCATTTGTGCTAAATCCATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCtag                                                                                          <  1:1072418/92‑3 (MQ=255)
  ggcttatttaaaaaaTGACGTGTCATTTGTGCTAAATCCATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCtag                                                                                          <  1:6190998/92‑3 (MQ=255)
  ggcttatttaaaaaaTGACGTGTCATTTGTGCTAAATCCATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCtag                                                                                          <  1:4794963/92‑3 (MQ=255)
  ggcttatttaaaaaaTGACGTGTCATTTGTGCTAAATCCATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCtag                                                                                          <  1:1477128/92‑3 (MQ=255)
  ggcttatttaaaaaaTGACGTGTCATTTGTGCTAAATCCATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCtag                                                                                          <  1:5431341/92‑3 (MQ=255)
  ggcttatttaaaaaaTGACGTGTCATTTGTGCTAAATCCATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCtag                                                                                          <  1:2059999/92‑3 (MQ=255)
    cttatttaaaaaaTGACGTGTCATTTGTGCTAAATCCATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCtaggt                                                                                        <  1:508584/94‑5 (MQ=255)
    cttatttaaaaaaTGACGTGTCATTTGTGCTAAATCCATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCTAGaa                                                                                        <  1:156643/94‑1 (MQ=255)
        tttaaaaaaTGACGTGTCATTTGTGCTAAGTCCATACCAGTTGCTATTCCTAATCTTCCAGTCATACTTTGTAACAGCATAGCAGATAAGCTAGAGAtta                                                                                    <  1:5598134/98‑3 (MQ=255)
        tttaaaaaaTGACGTGTCATTTGTGCTAAATCCATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCTAGAGAtta                                                                                    <  1:211099/98‑3 (MQ=255)
        tttaaaaaaTGACGTGTCATTTGTGCTAAATCCATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCTAGAGAtta                                                                                    <  1:1716980/98‑3 (MQ=255)
        tttaaaaaaTGACGTGTCATTTGTGCTAAATCCATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCTAGAGAtta                                                                                    <  1:4144486/98‑3 (MQ=255)
        tttaaaaaaTGACGTGTCATTTGTGCTAAATCCATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCTAGAGAtta                                                                                    <  1:2820443/98‑3 (MQ=255)
        tttaaaaaaTGACGTGTCATTTGTGCTAAATCCATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCTAGAGAtta                                                                                    <  1:4048606/98‑3 (MQ=255)
        tttaaaaaaTGACGTGTCATTTGTGCTAAATCCATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCTAGAGAtta                                                                                    <  1:3496140/98‑3 (MQ=255)
        tttaaaaaaTGACGTGTCATTTGTGCTAAATCCATACCAGTTGCTATTCCTAATCTTACAGTCATACGTTGTAACAGCATAGCAGATAAGCTAGAGAtta                                                                                    <  1:6532285/98‑3 (MQ=255)
                atGACGTGTCATTTGTGCTAAATCCATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCTAGAGATTAAGATTACg                                                                            <  1:4838100/100‑1 (MQ=255)
                 tgacGTGTCATTTGTGCTAAATCCATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCTAGAGATTAAGATTACgg                                                                           <  1:1130824/97‑2 (MQ=255)
                              gtgccaaatCCATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCTAGAGATTAAGATTACGAATAACAAGGtata                                                              >  1:6322559/9‑100 (MQ=255)
                                 ttAAATCCATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCTAGAGATTAAGATTACGAATAACAAGGTATAGcc                                                           <  1:6370366/99‑1 (MQ=255)
                                 cTAAATCCATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCTAGAGATTAAGATTACGAATAACAAGGTATAGcc                                                           <  1:4326315/100‑1 (MQ=255)
                                 cTAAATCCATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCTAGAGATTAAGATTACGAATAACAAGGTATAGcc                                                           <  1:3875748/100‑1 (MQ=255)
                                 cTAAATCCATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCTAGAGATTAAGATTACGAATAACAAGGTATAGcc                                                           <  1:1019785/100‑1 (MQ=255)
                                 cTAAATCCATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCTAGAGATTAAGATTACGAATAACAAGGTATAGcc                                                           <  1:3437372/100‑1 (MQ=255)
                                 cTAAATCCATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCTAGAGATTAAGATTACGAATAACAAGGTATAGcc                                                           <  1:130597/100‑1 (MQ=255)
                                 cTAAATCCATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCTAGAGATTAAGATTACGAATAACAAGGTATAGcc                                                           <  1:1627972/100‑1 (MQ=255)
                                 cTAAATCCATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCTAGAGATTAAGATTACGAATAACAAGGTATAGcc                                                           <  1:642346/100‑1 (MQ=255)
                                 cTAAATCCATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCTAGAGATTAAGATTACGAATAACAAGGTATAGcc                                                           <  1:6049483/100‑1 (MQ=255)
                                 cTAAATCCATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCTAGAGATTAAGATTACGAATAACAAGGTATAGcc                                                           <  1:3416972/100‑1 (MQ=255)
                                   aaatCCATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCTAGAGATTAAGATTACGAATAACAAGGTATAGCCat                                                         <  1:3068458/97‑1 (MQ=255)
                                   aaatCCATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCTAGAGATTAAGATTACGAATAACAAGGTATAGCCat                                                         <  1:6091393/97‑1 (MQ=255)
                                   aaatCCATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCTAGAGATTAAGATTACGAATAACAAGGTATAGCCat                                                         <  1:321531/97‑1 (MQ=255)
                                   aaatCCATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCTAGAGATTAAGATTACGAATAACAAGGTATAGCCat                                                         <  1:6500136/97‑1 (MQ=255)
                                   aaatCCATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCTAGAGATTAAGATTACGAATAACAAGGTATAGCCat                                                         <  1:5486109/97‑1 (MQ=255)
                                   aaatCCATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCTAGAGATTAAGATTACGAATAACAAGGTATAGCCat                                                         <  1:824628/97‑1 (MQ=255)
                                    aatCCATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCTAGAGATTAAGATTACGAATAACAAGGTATAGCCata                                                        <  1:5946143/98‑1 (MQ=255)
                                    aGTCCATACCTGTTGCTATTCCTAATCTCACTGTCATGCTTTGAAGTAACATTGCTGATAAACTTGAAATAAGAATTACGAATAGCAAAGTATAGCCatg                                                        <  1:570839/100‑2 (MQ=255)
                                     atCCATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCTAGGGATTAAGATTACGAATAACAAGGTATAGCCatag                                                       <  1:6112021/99‑2 (MQ=255)
                                     atCCATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCTAGAGATTAAGATTACGAATAACAAGGTATAGCCatat                                                       <  1:5445037/99‑1 (MQ=255)
                                     atCCATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCTAGAGATTAAGATTACGAATAACAAGGTATAGCCatat                                                       <  1:5852156/99‑1 (MQ=255)
                                     atCCATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCTAGAGATTAAGATTACGAATAACAAGGTATAGCCatat                                                       <  1:900215/99‑1 (MQ=255)
                                     atCCATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCTAGAGATTAAGATTACGAATAACAAGGTATAGCCatat                                                       <  1:2498437/99‑1 (MQ=255)
                                     atCCATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCTAGAGATTAAGATTACGAATAACAAGGTATAGCCatat                                                       <  1:2404184/99‑1 (MQ=255)
                                     atCCATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCTAGAGATTAAGATTACGAATAACAAGGTATAGCCatag                                                       <  1:1049190/99‑2 (MQ=255)
                                     atCCATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCTAGAGATTAAGATTACGAATAACAAGGTATAGCCatag                                                       <  1:700843/99‑2 (MQ=255)
                                      tCCATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCTAGAGATTAAGATTACGAATAACAAGGTATAGCCATAtt                                                      <  1:1856705/100‑1 (MQ=255)
                                       ccATACCTGTTGCTATTCCTAATCTCACTGTCATGCTTTGAAGTAACATTGCTGATAAACTTGAAATAAGAATTACGAATAGCAAAGTATAGCCATATTg                                                     >  1:5788037/1‑100 (MQ=255)
                                       ccATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCTAGAGATTAAGATTACGAATAACAAGGTATAGCCATATTg                                                     <  1:6583611/100‑1 (MQ=255)
                                       ccATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCTAGAGATTAAGATTACGAATAACAAGGTATAGCCATATTg                                                     <  1:5182503/100‑1 (MQ=255)
                                       ccATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCTAGAGATTAAGATTACGAATAACAAGGTATAGCCATATTg                                                     <  1:6136139/100‑1 (MQ=255)
                                       ccATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCTAGAGATTAAGATTACGAATAACAAGGTATAGCCATATTg                                                     <  1:3042677/100‑1 (MQ=255)
                                       ccATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCTAGAGATTAAGATTACGAATAACAAGGTATAGCCATATTg                                                     <  1:2172047/100‑1 (MQ=255)
                                       ccATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCTAGAGATTAAGATTACGAATAACAAGGTATAGCCATATTg                                                     <  1:3363060/100‑1 (MQ=255)
                                       ccATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCTAGAGATTAAGATTACGAATAACAAGGTATAGCCATATTg                                                     <  1:3747221/100‑1 (MQ=255)
                                       ccATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCTAGAGATTAAGATTACGAATAACAAGGTATAGCCATATTg                                                     <  1:1591951/100‑1 (MQ=255)
                                       ccATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCTAGAGATTAAGATTACGAATAACAAGGTATAGCCATATTg                                                     <  1:4629306/100‑1 (MQ=255)
                                       ccATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCTAGAGATTAAGATTACGAATAACAAGGTATAGCCATATTg                                                     <  1:5394440/100‑1 (MQ=255)
                                       ccATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCTAGAGATTAAGATTACGAATAACAAGGTATAGCCATATTg                                                     <  1:4692843/100‑1 (MQ=255)
                                        cATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCTAGAGATTAAGATTACGAATAACAAGGTATAGCCATATTgg                                                    <  1:4161585/100‑1 (MQ=255)
                                        cATACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCTAGAGATTAAGATTACGAATAACAAGGTATAGCCATATTgg                                                    <  1:2221707/100‑1 (MQ=255)
                                         aTACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCTAGAGATTAAGATTACGAATAACAAGGTATAGCCATATTgct                                                   <  1:6253137/100‑3 (MQ=255)
                                         aTACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCTAGAGATTAAGATTACGAATAACAAGGTATAGCCATATTgcc                                                   <  1:2864383/100‑3 (MQ=255)
                                         aTACCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCTAGAGATTAAGATTACGAATAACAAGGTATAGCCATATTgag                                                   <  1:1994588/100‑3 (MQ=255)
                                           aCCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCTAGAGATTAAGATTACGAATAACAAGGTATAGCCATATTGTgct                                                 <  1:5020361/100‑2 (MQ=255)
                                           aCCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCTAGAGATTAAGATTACGAATAACAAGGTATAGCCATATTGTgcc                                                 <  1:5310511/100‑2 (MQ=255)
                                           aCCAGTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCTAGAGATTAAGATTACGAATAACAAGGTATAGCCATATTGTgca                                                 <  1:2601796/100‑2 (MQ=255)
                                            ccagTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCTAGAGATTAAGATTACGAATAACAAGGTATAGCCATATTGTgctt                                                <  1:5571666/97‑3 (MQ=255)
                                             cagTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCTAGAGATTAAGATTACGAATAACAAGGTATAGCCATATTgcggtgt                                               <  1:2474427/98‑7 (MQ=255)
                                              agTTGCTATTCCTAATCTTACAGTCATACTTTGTAACAGCATAGCAGATAAGCTAGAGATTAAGATTACGAATAACAAGGTATAGCCATATTGTgctagt                                              <  1:123319/99‑5 (MQ=255)
                                                         cTAATCTCACTGTCATGCTTTGAAGTAACATTGCTGATAAACTTGAAATAAGAATTACGAATAGCAAAGTATAGCCATATTGGGCGCCACCTTGCATTgg                                   <  1:1887713/100‑2 (MQ=255)
                                                                cACTGTCATGCTTTGAAGTAACATTGCTGATAAACTTGAAATAAGAATTACGAATAGCAAAGTATAGCCATATTGGGCGCCACCTTGCATTGATGTTATc                            >  1:3774498/1‑100 (MQ=255)
                                                                 catGTCATGCTTTGAAGTAACATTGCTGATAAACTTGAAATAAGAATTACGAATAGCAAAGTATAGCCATATTGGGCGCCACCTTGCATTGATGTTATcc                           >  1:3174111/3‑100 (MQ=255)
                                                                         gCTTTGAAGTAACATTGCTGGTAAACTTGAAATAAGAATTACGAATAGCAAAGTATAGCCATATTGGGCGCCACCTTGCATTGATGTTATCCAGTTTCCg                   <  1:5755486/100‑1 (MQ=255)
                                                                            ttGAAGTAACATTGCTGATAAACTTGAAATAAGAATTACGAATAGCAAAGTATAGCCATATTGGGCGCCGCCTTGCATTGATGTTATCCAGTTTCCGGGa                <  1:5983858/100‑1 (MQ=255)
                                                                              gAAGTAACATTGCTGATAAACTTGAAATAAGAATTACGAATAGCAAAGTATAGCCATATTGGGCGCCACCTTGCATTGATGTTATCCAGTTTCCGGGagc              <  1:5253280/100‑3 (MQ=255)
                                                                                         gCTGATAAACTTGAAATAAGAATTACGAATAGCAAAGTATAGCCATATTGGGCGCCACCTTGCATTGATGTTATCCAGTTTCCGGGATCCATGTAACCag   <  1:4946836/100‑2 (MQ=255)
                                                                                           |                                                                                                  
TAGGTCTTGATAAATAATGTCGTGTCATTTGAGCTAAGTCCATACCTGTTGCTATTCCTAATCTCACTGTCATGCTTTGAAGTAACATTGCTGATAAACTTGAAATAAGAATTACGAATAGCAAAGTATAGCCATATTGGGCGCCACCTTGCATTGATGTTATCCAGTTTCCGGGATCCATGTAACCAAC  >  NC_007793/1101237‑1101426

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑