Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	115,248	T→G	G565G (GGT→GGG)	SAUSA300_RS00540 →	AraC family transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	115,248	0	T	G	100.0%	44.4 / NA	19	G565G (GGT→GGG)	SAUSA300_RS00540	AraC family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (19/0);  total (19/0)

TACAAACGGCGAATATTTTAGAGGTGGTATCATCATTGAGCAATTATTAAAATTAAGTTCTAAAGTAGAGGGTATCGGTTATTGGTTGAATTATGATTTGCACGTCAGTCATTGTAAAAATGAACGGGATTATATGAATTCTATTGAACTATTTCATCAATATAATGGAAAACGTC  >  NC_007793/115170‑115345                                                                               |                                                                                                  taCAAACGGCGAATATTTTAGAGGTGGTATCATCATTGAGCAATTATTAAAATTAAGTTCTAAAGTAGAGGGTATCGGGTATTGGTTGAATTATGATTTg                                                                              >  1:1580129/1‑100 (MQ=255)                        gtggtATCATCATTGAGCAATTATTAAAATTAAGTTCTAAAGTAGAGGGTATCGGGTATTGGTTGAATTATGATTTGCACGTCAGTCATTGTAAAAATGa                                                       >  1:1900072/1‑100 (MQ=255)                                 tcatTGAGCAATTATTAAAATTAAGTTCTAAAGTAGAGGGTATCGGGTATTGGTTGAATTATGATTTGCACGTCAGTCATTGTAAAAATGAACGGGATta                                              >  1:2197108/1‑100 (MQ=255)                                  catTGAGCAATTATTAAAATTAAGTTCTAAAGTAGAGGGTATCGGGTATTGGTTGAATTATGATTTGCACGTCAGTCATTGTAAAAATGAACGGGaccag                                             >  1:5493637/1‑96 (MQ=255)                                  catTGAGCAATTATTAAAATTAAGTTCTAAAGTAGAGGGTATCGGGGATTGGTTGAATTCTGATTTGCACGTCAGTCATTGTAAAAATGAACGGGATtat                                             >  1:2875455/1‑100 (MQ=255)                                                            cTAAAGTAGAGGGTATCGGGTATTGGTTGAATTATGATTTGCACGTCAGTCATTGTAAAAATGAACGGGATTATATGAATTCTATTGAACTATTtcatca                   >  1:5610266/1‑100 (MQ=255)                                                              aaaGTAGAGGGTCTCGGGTATTGGTTGAATTATGATTTGCACGTCAGTCATTGTAAAAATGAACGGGATTATATGAATTCTATTGAACTATTTCATCAat                 >  1:6123231/1‑100 (MQ=255)                                                              aaaGTAGAGGGTATCGGGTATTGGTTGAATTATGATTTGCACGTCAGTCATTGTAAAAATGAACGGGATTATATGACTTCTATTGAACTATTTCATCAat                 >  1:640571/1‑100 (MQ=255)                                                              aaaGTAGAGGGTATCGGGTATTGGTTGAATTATGATTTGCACGTCAGTCATTGTAAAAATGAACGGGATTATATGAATTCTATTGGACTATTTCATCAat                 >  1:4994205/1‑100 (MQ=255)                                                              aaaGTAGAGGGTATCGGGTATTGGTTGAATTATGATTTGCACGTCAGTCATTGTAAAAATGAACGGGATTATATGAATTCTATTGAACTATTTCATCAat                 >  1:5691686/1‑100 (MQ=255)                                                                         tATCGGGTATTGGTTGAATTATGATTTGCACGTCAGTCATTGTAAAAATGAACGGGATTATATGAATTCTATTGAACTATTTCATCAATATAATGGgaaa      >  1:1022909/1‑100 (MQ=255)                                                                          ctCGGGTATTGGTTGAATTATGATTTGCACGTCAGTCATTGTAAAAATGAACGGGATTATATGAATTCTATTGAACTATTTCATCAATATAATGGAAAAc     >  1:2372295/2‑100 (MQ=255)                                                                          aTCGGGTATTGGTTGAATTATGATTTGCACGTCAGTCATTGTAAAAATGTACGGGATTATATGAATTCTATTGAACTATTTCATCAATATAATGGAAAAc     >  1:2027959/1‑100 (MQ=255)                                                                          aTCGGGTATTGGTTGAATTATGATTTGCACGTCAGTCATTGTAAAAATGAACGGGATTATATGAATTCTATTGAACTATTTCATCCATATAATGGAAAAc     >  1:4808890/1‑100 (MQ=255)                                                                           gcGGGTATTGGTTGAATTATGATTTGCACGTCAGTCATTGTAAAAATGAACGGGATTATATGAATTCTATTGAACTATTTCATCAATATAATGGAAAACg    >  1:4406579/2‑100 (MQ=255)                                                                            cGGGTATTGGTTGAATTATGATTTGCACGTCAGTCATTGTAAAAATGAACGGGATTATATGAATTCTATTGAACTATTTCATCAATATAATGGAAAACGt   >  1:3657525/1‑100 (MQ=255)                                                                            cGGGTATTGGTTGAATTATGATTTGCACGTCAGTCATTGTAAAAATGAACGGGATTATATGAATTCTATTGAACTATTTCATCAATATAATGGAAAACGt   >  1:680332/1‑100 (MQ=255)                                                                            cGGGTATTGGATGAATTATGATTTGCACGTCAGTCATTGTAAAAATGAACGGGATTATATGAATTCTATTGAACTATTTCATCAATATAATGGAAAACGt   >  1:3739345/1‑100 (MQ=255)                                                                            cGGGTATT‑GTTGAATTATGATTTGCACGTCAGTCATTGTAAAAATGAACGGGATTATATGAATTCTATTGAACTATTTCATCAATATAATGGAAAACGTc  >  1:714395/1‑100 (MQ=255)                                                                               |                                                                                                  TACAAACGGCGAATATTTTAGAGGTGGTATCATCATTGAGCAATTATTAAAATTAAGTTCTAAAGTAGAGGGTATCGGTTATTGGTTGAATTATGATTTGCACGTCAGTCATTGTAAAAATGAACGGGATTATATGAATTCTATTGAACTATTTCATCAATATAATGGAAAACGTC  >  NC_007793/115170‑115345

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑