Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,074,815	G→A	D71N (GAT→AAT)	SAUSA300_RS05275 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,074,815	0	G	A	100.0%	82.5 / NA	31	D71N (GAT→AAT)	SAUSA300_RS05275	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/3);  total (28/3)

GGTGGTAAAAAAGTTGATACACATGTTCATAATGTGTGGGAATTTCTGAATGCATTTTTCAGTGAAATTATTAAAGTAATGAGTCGATTTATAGGTGATTTCCCTATAGTTAGTGCAATTGTGATAATTATATTCGGTATTTTAGTTATGTTGATTGGTCATACATTACTTAGAACTATTAAGTATGACT  >  NC_007793/1074719‑1074908                                                                                                 |                                                                                              ggtggtAAAAAAGTTGATACACATGTTCATAATGTGTGGGAATTTCTGAATGCATTTTTCAGTGAAATTATTAAAGTAATGAGTCGATTTATAGGTAAtt                                                                                            >  1:2240680/1‑100 (MQ=255)  gtggtAAAAAAGTTGATACACATGTTCATAATGTGTGGGAATTTCTGAATGCATTTTTCAGTGAAATTATTAAAGTAATGAGTCGATTTATAGGTAAttt                                                                                           >  1:5202753/1‑100 (MQ=255)    ggtAAAAAAGTTGATACACATGTTCATAATGTGTGGGAATTTCTGAATGCATTTTTCAGTGAAATTATTAAAGTAATGAGTCGATTTATAGGTAATTTcc                                                                                         >  1:814855/1‑100 (MQ=255)    ggtAAAAAAGTTGATACACATGTTCATAATGTGTGGGAATTTCTGAATGCATTTTTCAGTGAAATTATTAAAGTAATGAGTCGATTTATAGGTAATTTcc                                                                                         >  1:6404362/1‑100 (MQ=255)                 aTACACATGTTCATAATGTGTGGGAATTTCTGAATGCATTTTTCAGTGAAATTATTAAAGTAATGAGTCGATTTATAGGTAATTTCCCTATAGTTAGTGc                                                                            <  1:114159/100‑1 (MQ=255)                  tACACATGTTCATAATGTGTGGGAATTTCTGAATGCATTTTTCAGTGAAATTATTAAAGTAATGAGTCGATTTATAGGTAATTTCCCTATAGTTAGTGCa                                                                           <  1:20752/100‑1 (MQ=255)                           tCATAATGTGTGGGAATTTCTGAATGCATTTTTCAGTGAAATTATTAAAGTAATGAGTCGATTTATAGGTAATTTCCCTATAGTTAGTGCAATTGTGATa                                                                  >  1:4237044/1‑100 (MQ=255)                            cATAATGTGTGGGAATTTCTGAATGCATTTTTCAGTGAAATTATTAAAGTAATGAGTCGATTTATAGGTAATTTCCCTATAGTTAGTGCAATTGTGATaa                                                                 >  1:5273291/1‑100 (MQ=255)                               aaTGTGTGGGAATTTCTGAATGCATTTTTCAGTGAAATTATTAAAGTAATGAGTCGATTTATAGGTAATTTCCCTATAGTTAGTGCAATTGTGATAATta                                                              >  1:2169143/1‑100 (MQ=255)                                ctgtgtgGGAATTTCTGAATGCATTTTTCAGTGAAATTATTAAAGTAATGAGTCGATTTATAGGTAATTTCCCTATAGTTAGTGCAATTGTGATAATtat                                                             >  1:1841317/2‑100 (MQ=255)                                  ctgtgGGAATTTCTGAATGCATTTTTCAGTGAAATTATTAAAGTAATGAGTCGATTTATAGGTAATTTCCCTATAGTTAGTGCAATTGTGATAATTatat                                                           >  1:5378874/2‑100 (MQ=255)                                   tgtgGGAATTTCTGAATGCATTTTTCAGTGAAATTATTAAAGTAATGAGTCGATTTATAGGTAATTTCCCTATAGTTAGTGCGATTGTGATAATTATAtt                                                          >  1:4634227/1‑100 (MQ=255)                                   tgtgGGAATTTCTGAATGCATTTTTCAGTGAAATTATTAAAGTAATGAGTCGATTTATAGGTAATTTCCCTATAGTTAGTGCAATTGTGATAATTATAtt                                                          >  1:1565281/1‑100 (MQ=255)                                   ctggggAATTTCTGAATGCATTTTTCAGTGAAATTATTAAAGTAATGAGTCGATTTATAGGTAATTTCCCTATAGTTAGTGCAATTGTGATAATTATAtt                                                          >  1:6643711/4‑100 (MQ=255)                                    gtgGGAATTTCTGAATGCATTTTTCAGTGAAATTATTAAAGTAATGAGTCGATTTATAGGTAATTTTCCTATAGTTAGTGCAATTGTGATAATTATATTc                                                         >  1:5841853/1‑100 (MQ=255)                                         aaTTTCTGAATGCATTTTTCAGTGAAATTATTAAAGTAATGAGTCGATTTATAGGTAATTTCCCTATAGTTAGTGCAATTGTGATAATTATATTCGGTAt                                                    <  1:956611/100‑1 (MQ=255)                                              cTGAATGCATTTTTCAGTGAAATTATTAAAGTAATGAGACGATTTATAGGTAATTTCCCTATAGTTAGTGCAATTGTGATAATTATATTCGGTATTTTAg                                               >  1:6598334/1‑100 (MQ=255)                                                taaTGCATTTTTCAGTGAAATTATTAAAGTAATGAGTCGATTTATAGGTAATTTCCCTATAGTTAGTGCAATTGTGATAATTATATTCGGTATTTTAGtt                                             >  1:84889/2‑100 (MQ=255)                                                gAATGCATTTTTCAGTGAAATTATTCAAGTAATGAGTCGATTTATAGGTAATTTCCCTATAGTTAGTGCAATTGTGATAATTATATTCGGTATTTTAGtt                                             >  1:6257100/1‑100 (MQ=255)                                                gAATGCATTTTTCAGTGAAATTATTAAAGTAATGAGTCGATTTATAGGTAATTTCCCTATAGTTAGTGCAATTGTGATAATTATATTCGGTATTTTAGtt                                             >  1:473791/1‑100 (MQ=255)                                                gAATGCATTTTTCAGTGAAATTATTAAAGTAATGAGTCGATTTATAGGTAATTTCCCTATAGTTAGTGCAATTGTGATAATTATATTCGGTATTTTAGtt                                             >  1:5348162/1‑100 (MQ=255)                                                gAATGCATTTTTCAGTGAAATTATTAAAGTAATGAGTCGATTTATAGGTAATTTCCCTATAGTTAGTGCAATTGTGATAATTATATTCGGTATTTTAGtt                                             >  1:6321992/1‑100 (MQ=255)                                                                          aaGTAATGAGTCGATTTATAGGTAATTTCCCTATAGTTAGTGCAATTGTGATAATTATATTCGGTATTTTAGTTATGTTGATTGGTCATACATTACTTAg                   >  1:944005/1‑100 (MQ=255)                                                                                 caGTCGATTTATAGGTAATTTCCCTATAGTTAGTGCAATTGTGATAATTATATTCGGTATTTTAGTTATGTTGATTGGTCATACATTACTTAGAACTAtt            >  1:4060252/2‑100 (MQ=255)                                                                                           ataGGTAATTTCCCTATAGTTGGTGCAATTGTGATAATTATATTCGGTATTTTAGTTATGTTGATTGGTCATACATTACTTAGAACTATTAAGTATGACt  >  1:4053848/1‑100 (MQ=255)                                                                                           ataGGTAATTTCCCTATAGTTAGTGCAATTGTGATAATTATATTCGGTATTTTAGTTATGTTGATTGGTCATACATTACTTAGAACTATTAAGTATGACt  >  1:432230/1‑100 (MQ=255)                                                                                           ataGGTAATTTCCCTATAGTTAGTGCAATTGTGATAATTATATTCGGTATTTTAGTTATGTTGATTGGTCATACATTACTTAGAACTATTAAGTATGACt  >  1:3989288/1‑100 (MQ=255)                                                                                           ataGGTAATTTCCCTATAGTTAGTGCAATTGTGATAATTATATTCGGTATTTTAGTTATGTTGATTGGTCATACATTACTTAGAACTATTAAGTATGACt  >  1:2245962/1‑100 (MQ=255)                                                                                           ataGGTAATTTCCCTATAGTTAGTGCAATTGTGATAATTATATTCGGTATTTTAGTTATGTTGATTGGTCATACATTACTTAGAACTATTAAGTATGACt  >  1:2156303/1‑100 (MQ=255)                                                                                           ataGGTAATTTCCCTATAGTTAGTGCAATTGTGATAATTATATTCGGTATTTTAGTTATGTTGATTGGTCATACATTACTTAGAACTATTAAGTATGACt  >  1:1712541/1‑100 (MQ=255)                                                                                           ataGGTAATTTCCCTATAGTTAGTGCAATTGTGATAATTATATTCGGTATTTTAGTTATGTTGATTGGTCATACATTACTTAGAACTATTAAGTATGACt  >  1:1343043/1‑100 (MQ=255)                                                                                                 |                                                                                              GGTGGTAAAAAAGTTGATACACATGTTCATAATGTGTGGGAATTTCTGAATGCATTTTTCAGTGAAATTATTAAAGTAATGAGTCGATTTATAGGTGATTTCCCTATAGTTAGTGCAATTGTGATAATTATATTCGGTATTTTAGTTATGTTGATTGGTCATACATTACTTAGAACTATTAAGTATGACT  >  NC_007793/1074719‑1074908

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑